Size Fractions Research Articles

Polyethylene Terephthalate Microplastics Generated from Disposable Water Bottles Induce Interferon Signaling Pathways in Mouse Lung Epithelial Cells.

Microplastics (MPs) are present in ambient air in a respirable size fraction; however, their potential impact on human health via inhalation routes is not well documented. In the present study, methods for a lab-scale generation of MPs from regularly used and littered plastic articles were optimized. The toxicity of 11 different types of MPs, both commercially purchased and in-lab prepared MPs, was investigated in lung epithelial cells using cell viability, immune and inflammatory response, and genotoxicity endpoints. The underlying mechanisms were identified by microarray analysis. Although laborious, the laboratory-scale methods generated a sufficient quantity of well characterized MPs for toxicity testing. Of the 11 MPs tested, the small sized polyethylene terephthalate (PETE) MPs prepared from disposable water bottles induced the maximum toxicity. Specifically, the smaller size PETE MPs induced a robust activation of the interferon signaling pathway, implying that PETE MPs are perceived by cells by similar mechanisms as those employed to recognize pathogens. The PETE MPs of heterogenous size and shapes induced cell injury, triggering cell death, inflammatory cascade, and DNA damage, hallmark in vitro events indicative of potential in vivo tissue injury. The study establishes toxicity of specific types of plastic materials in micron and nano size.

Vein of Marshall ethanol infusion for recurrent atrial fibrillation in patients with durably isolated pulmonary veins.

Vein of Marshall (VoM) ethanol ablation has a proven benefit in patients with persistent atrial fibrillation (AF) undergoing index procedure; however, its role in repeat ablation is unknown. We sought to evaluate the benefit of empiric VoM ethanol ablation in addition to posterior wall isolation (PWI) during the repeat procedure in patients with durable pulmonary vein (PV) isolation from prior ablation. Twenty-three patients (age 67.1 + / - 7.4, 74% males) who received empiric VoM ethanol infusion in addition to PWI were matched for age, gender, ejection fraction, and left atrial size with forty-six patients receiving empiric PWI alone. All patients in the study group underwent additional ablation on mitral isthmus to complete the lateral mitral isthmus line. Additional ablation was based on program and trigger stimulation. Primary outcome was freedom from AF after a blanking period of 3months by qualification of symptoms, EKG, wearable, or implantable monitor or device. The study group had a higher average BMI (35.07 + / - 8.98 vs. 30.85 + / - 5.65, p = 0.033) and rate of persistent AF (83.0% vs. 54.3%, p = 0.029) versus the control. The 1-year AF-free survival for the study and control groups was 20 (86.96%) and 28 (60.1%) patients (p = 0.027). Cox proportional hazard regression analysis showed a significant reduction in AF recurrence in the study group (HR 0.25, 95% CI 0.073-0.843, p = 0.026). Among patients undergoing repeat catheter ablation for recurrent AF with durably isolated PVs, the addition of VoM ethanol infusion increased the likelihood of remaining free from AF at 12months.

Quantifying the biogenic silica production of picoplankton in the oligotrophic ocean: A case study in the South China Sea

Silicon (Si) utilization is not limited to eukaryotes. Recent research has suggested that the pattern of a large contribution of picocyanobacteria to biogenic silica (bSi) stocks might be widespread in the oligotrophic open ocean. We are the first to measure the size-fractionated bSi standing stocks and production rates in the oligotrophic South China Sea (SCS), which has obvious characteristics of oligotrophic waters. The 150 m integrated bSi standing stocks in the pico-sized fractions averaged 23 % of the total; the contribution of picoplankton to the total bSi production rate was 44 %. Interestingly, our estimated contributions of Synechococcus alone to the <2 μm bSi standing stock and < 2 μm bSi production rates averaged 14 % and 66 %, respectively, indicating that the significant and persistent contribution of bSi was strongly associated with marine picocyanobacteria. Furthermore, the dynamic changes in nutrient concentrations, especially in DIN and DIP, also potentially affected the variability in picoplankton bSi stocks and production rates, while the effects of temperature and salinity were not obvious. In this study, we have provided new information on measurable bSi in the picoplankton size fraction and its production rate in the SCS. We have demonstrated that picoplankton contributes a measurable, and at times significant, proportion to both the total bSi standing stock and its production rate in the SCS. A high silicon content within picocyanobacteria has important implications for understanding both their ecology and their contribution to biogeochemistry.

Tectonic and erosional controls of sediment residence time in small catchments draining an active mountain range: Evidence from uranium comminution age

Sediment residence time (SRT) is a key parameter of the Earth’s surface system, bridging the realms of sediment transport, biogeochemical cycles, sedimentary archives, and watershed management. SRT in mountainous catchments includes the time particles have spent in the subsurface weathering zone, which has been challenging to measure thus limiting our understanding of the magnitude and controlling factors of SRT in steep terrains. In this study, we apply the 234U/238U activity ratio, (234U/238U), which reveals particle comminution age, to estimate SRTs in 32 catchments (drainage area of 6.79–143.67 km2) draining an active mountain range at the eastern margin of the Tibetan Plateau. The uranium comminution age allows us to account for the time that particles have spent in the weathering zone. The sampled catchments span a range of erosional, topographic, climatic and lithologic conditions, providing a unique opportunity to explore the controlling factors on SRTs. We measured the (234U/238U) of the 20–25 µm size fraction of the collected riverbed sediment samples and used the results to calculate particle comminution ages, which are used to approximate SRTs of fine sediment in the catchment. We then investigated the relationships between estimated SRTs and denudation rates, topography, climate, and lithology to assess controls on SRTs. The estimated SRTs range from 5.0 to 149.2 ka, and exhibit strong negative correlations with the 10Be-derived denudation rates and metrics for landscape steepness (relief, gradient, and channel steepness), with weak-to-moderate correlations with climatic and lithologic conditions. By combining the SRTs and denudation rates and assuming a steady-state weathering zone, we estimate a catchment-average comminution depth of 1.98 to 28.7 m, comparable to the depth of weathering zone estimated from modeling studies. Considering a topographic steady-state where denudation rates are in equilibrium with uplift rates, our results suggest that tectonic uplift exerts a primary control on the SRT in active mountain ranges, such that faster uplift rates shorten SRTs. Our findings suggest that tectonic uplift could regulate the temporal duration of chemical weathering by limiting the SRTs of fresh minerals in mountainous regions, sustaining a ‘kinetically-limited’ weathering scenario.

Impact of power input in pulp conditioning on coal flotation and real-time characterization by focused beam reflectance measurement (FBRM)

ABSTRACT Pulp conditioning is a necessary procedure in the coal flotation industry, and optimizing the power input of such processes is in favor of improving flotation efficiency and reducing costs. This paper applied a pulp conditioning system combined with the focused beam reflectance measurement (FBRM) to investigate the impact of power input on coal flotation. Under the same energy input, the highest flotation efficiency of 50.09% was achieved in a pulp conditioning pattern of 1800 rpm-3 min, but the flotation efficiency reached only 36.13% in 1200 rpm-7.2 min. The kerosene collector can disperse into large numbers of droplets that exceed 2000 counts/s at size fractions of −10 μm and 20–50 μm in high power input and achieve better adsorption on coal. In the pulp conditioning process, the coal particle numbers of −50 μm size fraction decreased significantly from 15,000 counts/s to nearly 8000 counts/s at 32.3 W power input and coarse aggregates of 50–1000 μm size were detected. Under high power input conditions caused by high agitation speed, extra energy contributed validly to the kinetic energy of coal particles/collector droplets and the generation of oil-water and oil–coal interfaces. The shear flocculation behavior induced by the high power inputs in pulp conditioning is favorable for the flotation recovery of fine and ultra-fine particles.

Tellurium Enrichment in Copper Tailings: A Mineralogical and Processing Study

As the global demand for tellurium (Te) increases, it is crucial to develop efficient recovery methods that consider existing supply streams. This research combines gravity separation and froth flotation processes to enhance the recovery of Te minerals from tailings produced during the beneficiation of copper porphyry ores. Prior to processing, a systematic and comprehensive characterization study of copper tailing (CT) samples was conducted to examine the deportment of Te minerals in different mineral phases and to understand their locking and liberation behavior. Characterization techniques included inductively coupled plasma mass spectrometry (ICP-MS) and TESCAN’s integrated mineral analysis (TIMA). Copper tailing characterization showed that minerals with gold (Au), silver (Ag), bismuth (Bi), and Te were present in various forms, including native Au, electrum, tellurides, and sulfosalts. TIMA revealed that &gt;90% of these minerals were primarily hosted in pyrite as less than 10 µm inclusions in the CT. TIMA also revealed that Te minerals exhibited fine-grained liberation of less than 20 μm. Moreover, TIMA results showed that &gt;80% of mica and other silicate minerals were concentrated in size fractions &lt; 38 μm, suggesting that desliming processes would positively impact Te enrichment. The results from the processing tests showed a Te recovery rate of ~77% and a Te enrichment ratio of 13 when using the combination of gravity separation and froth flotation at 90 g/t xanthate collector and 50 g/t glycol frother. The findings from this study show a significant potential for Te recovery from unconventional sources if appropriate physical beneficiation approaches are adopted.

A new investigation on graphene-reinforced 304L nanocomposite made by additive manufacturing process: Microstructure and tribology study

In this study, selective laser melting (SLM), as an additive manufacturing method, was applied to produce graphene/304L nanocomposites with various graphene particle sizes and contents. The effect of the graphene particle size (1 μm and 50 nm) and volume fraction on the microstructure behaviors, constitutional phases, mechanical performance, and wear characteristics of the SLM-processed nanocomposite specimens was studied. It was concluded that the reinforcing particle content and size play a remarkable impact on the densification features and the densification level improvs when the fine starting reinforcing particles are used, due to the development of the reinforcement–matrix wettability. In addition, the microstructure refinement and increased hardness values occurred in the SLM-processed nanocomposite system with the addition of reinforcing particles (both sizes). The wear examination indicated that the coefficient of friction (COF), wear rate, and mass loss decreased due to the reinforcement prominent effect, namely grain refinement and grain-boundary strengthening. According to surface topography analysis, the depth of the groove in the processed sample made by fine graphene reinforcement is lower than the other sample.

Turbulent pipe flow with spherical particles: Drag as a function of particle size and volume fraction

Suspensions of finite-size solid particles in a turbulent pipe flow are found in many industrial and technical flows. Due to the ample parameter space consisting of particle size, concentration, density and Reynolds number, a complete picture of the particle–fluid interaction is still lacking. Pressure drop predictions are often made using viscosity models only considering the bulk solid volume fraction. For the case of turbulent pipe flow laden with neutrally buoyant spherical particles, we investigate the pressure drop and overall drag (friction factor), fluid velocity and particle distribution in the pipe. We use a combination of experimental (MRV) and numerical (DNS) techniques and a continuum flow model. We find that the particle size and the bulk flow rate influence the mean fluid velocity, velocity fluctuations and the particle distribution in the pipe for low flow rates. However, the effects of the added solid particles diminish as the flow rate increases. We created a master curve for drag change compared to single-phase flow for the particle-laden cases. This curve can be used to achieve more accurate friction factor predictions than the traditional modified viscosity approach that does not account for particle size.

Myocardial strain combined with clinical risk factors in the prediction of in-hospital heart failure among patients with ST-segment elevation myocardial infarction

To investigate the predictive value of myocardial strain derived from cardiac magnetic resonance (CMR) combined with clinical indicators for in-hospital heart failure (HF) in STEMI patients. In all, 139 STEMI patients were included, with 28 in the heart failure group and 111 in the non-HF group, and clinical and laboratory data were collected. Left ventricular (LV) global radial strain (GRS), global longitudinal strain (GLS), global circumferential strain (GCS), left ventricular ejection fraction (LVEF), stroke volume (SV), and infarct size (IS) were assessed by CMR. The HF group had worse GRS, GLS, GCS, LVEF, SV, larger IS, longer symptom to balloon time (SBT) and higher levels of high-sensitivity C-reactive protein (hs-CRP) and neutrophil percentage (N%) than the non-HF group (P<0.05). There was a strong correlation between GRS and LVEF (r=0.741, P<0.001). After adjustment for CMR and clinical risk factors, GRS<15.6%, LVEF<37.7%, SBT>350 min, hs-CRP>11.45 mg/L, and N%>74% were independently associated with HF. Clinical model (SBT>350 min+hs-CRP>11.45 mg/L+N%>74%) were associated with a lower diagnostic accuracy for predicting in-hospital HF than GRS+clinical co-model and LVEF+clinical co-model (P<0.05), respectively. There was no significant difference in the area under the curve (AUC) between GRS+clinical co-model and LVEF+clinical co-model (P=0.620): AUC for clinical model=0.824, AUC for GRS+clinical co-model=0.895, and AUC for LVEF+clinical co-model=0.907. GRS may be effective in predicting in-hospital heart failure after STEMI compared to LVEF, a classical cardiac function parameter, and its combination with clinical risk factors, especially SBT, hs-CRP, and N%, may provide further evidence for early prognostic assessment.

Microorganisms Involved in Methylmercury Demethylation and Mercury Reduction are Widely Distributed and Active in the Bathypelagic Deep Ocean Waters.

The ocean's mercury (Hg) content has tripled due to anthropogenic activities, and although the dark ocean (>200 m) has become an important Hg reservoir, concentrations of the toxic and bioaccumulative methylmercury (MeHg) are low and therefore very difficult to measure. As a consequence, the current understanding of the Hg cycle in the deep ocean is severely data-limited, and the factors controlling MeHg, as well as its transformation rates, remain largely unknown. By analyzing 52 globally distributed bathypelagic deep-ocean metagenomes and 26 new metatranscriptomes from the Malaspina Expedition, our study reveals the widespread distribution and expression of bacterial-coding genes merA and merB in the global bathypelagic ocean (∼4000 m depth). These genes, associated with HgII reduction and MeHg demethylation, respectively, are particularly prevalent within the particle-attached fraction. Moreover, our results indicate that water mass age and the organic matter composition shaped the structure of the communities harboring merA and merB genes living in different particle size fractions, their abundance, and their expression levels. Members of the orders Corynebacteriales, Rhodobacterales, Alteromonadales, Oceanospirillales, Moraxellales, and Flavobacteriales were the main taxonomic players containing merA and merB genes in the deep ocean. These findings, together with our previous results of pure culture isolates of the deep bathypelagic ocean possessing the metabolic capacity to degrade MeHg, indicated that both methylmercury demethylation and HgII reduction likely occur in the global dark ocean, the largest biome in the biosphere.

Grain Size in Landscapes

Earth's terrestrial topography evolves in response to the interaction of tectonics, climate, and lithology. Recent discoveries suggest that the grain size of sediments produced on hillslopes and transported through river networks is key to understanding these interactions. Hillslope grain size varies systematically with erosion rate and residence time, the degree of chemical and physical weathering, and the fracture density and susceptibility to weathering of rock. Variations in initial grain size strongly influence the spatial evolution of grain size distributions as particles mix and wear during downstream transport through channel networks. In rivers, the size and flux of the coarse fraction of the sediment load control the rate of incision into bedrock and thus govern channel slope and ultimately the relief of actively eroding landscapes. These relationships suggest that a primary way that tectonics, climate, and lithology influence landscape evolution is through their controls on sediment grain size. ▪Recent research reveals the central role of sediment grain size in controlling bedrock river morphodynamics, linking grain size to channel slope and topographic relief.▪Tectonics, climate, and lithology govern the size of sediments produced on hillslopes; hence, grain size mediates their influence on landscape evolution.▪Feedbacks linking sediment grain size, topography, weathering, erosion, and sediment transport provide new opportunities for advances in Earth surface science.

Micromechanism insights and constitutive modeling for deformation in a novel high manganese austenitic steel

We explore the mechanical behavior and microstructural evolution of a novel high manganese austenitic steel (HMAS), extensively applied in underground engineering and structural construction. Our investigation focuses on two variants of HMAS, namely HMAS-1.9 and HMAS-10, which exhibit distinct yield strengths and elongation behaviors. Electron backscatter diffraction (EBSD), transmission electron microscopy (TEM) and X-ray diffraction (XRD) were employed to elucidate the evolution of microstructures during plastic deformation. Based on these experimental results, we developed a new phenomenological multiscale constitutive model that captures the mechanical behavior of HMAS, integrating micromechanisms and microstructural evolutions. Optimized with the MATLAB genetic algorithm toolbox, this model was successfully implemented in a uniaxial tensile finite element model (FEM) within ABAQUS, proving its efficacy in predicting both macroscopic flow stress and microstructural evolutions. Our findings highlight the important roles of initial grain size, dislocation density, and twin fraction in determining the mechanical properties of HMAS. Throughout the deformation process, both HMAS variants demonstrate similar plastic deformation behaviors, characterized by comparable rates of increase in dislocation density and twinning fraction, accompanied by a consistent trend of grain refinement. These findings not only deepen our understanding of the complex relationship between microstructure and mechanical properties of the HMAS steel, but also provide an effective multiscale constitutive modeling approach for predicting its deformation behavior.

Size and polarity fractions of mobile organic matter from manure affect the sorption of sulfadiazine, caffeine and atenolol in soil

Waste-derived organics introduced to soils along with pharmaceutical active compounds (PhAC) are a crude mixture of compounds occurring in various size and polarity fractions. They affect the sorption of PhACs to soil; however, the relevant knowledge is still insufficient. The effects of different size and polarity fractions of manure-derived mobile organic matter (<63 µm) on the sorption of sulfadiazine, caffeine and atenolol to five topsoils were investigated. Mobilization of the PhACs was strongest in the presence of dissolved organic matter (mDOM, <0.45 µm), with a reduction of Kd of sulfadiazine, caffeine and atenolol by mean factors of 0.66, 0.57 and 0.41, respectively. The mobilizing effects of colloidal organic matter (0.45–10 µm) were slightly smaller. Fine particulate organic matter (10–63 µm) reduced the sorption of the PhACs in slightly acidic soils (pH 6.0), but increased it in strongly acidic soil (pH 4.3). Furthermore, hydrophobic (HO-mDOM) and hydrophilic (HI-mDOM) fractions of mDOM reduced the sorption capacity but increased the sorption nonlinearity of PhACs in soils. Effects of HO-mDOM and HI-mDOM were PhAC specific. It is suggested to consider the varying impacts of mobile fractions in animal manure and/or treated wastewater in evaluating the fate and environmental relevance of associated PhACs.

Elemental and particle size fractionation during the transport of Eu(III)-silicate colloids in water-saturated porous media

Actinides (An)-bearing colloids could facilitate An migration in the environment. However, little is known about the transport behavior of An(III)-silicate colloids, which are readily formed by the reaction of An3+ with silicic acid under environmental conditions. Column experiments were conducted to investigate the transport of Eu(III)-silicate colloids (chemical analog of An(III)-silicate colloids) in water-saturated porous media as a function of pH, ionic strength (IS) and the presence of fulvic acid (FA). The results showed that colloid transport was more favorable at relatively low IS (≤ 50 mM) and high pH levels (pH ≥ 7). The presence of FA (5−10 mg/L) significantly enhanced the colloid transport. Under high IS (≥ 100 mM), the transport feature of colloids was turned from blocking to ripening due to the on-going aggregation of colloids. Additionally, an interesting elemental fractionation, i.e., a discrepancy in the breakthrough curves (BTCs) with respect to the C/C0 values of Si and Eu, was observed in the IS of 100−500 mM. A detailed investigation indicated that the elemental fractionation could be attributed to the partial Si dissolution of the colloids, the heterogeneity of the colloid size and element composition, and particle size fractionation during colloid transport. Extended Derjaguin-Landau-Verwey-Overbeek interaction energy calculations and convective-dispersive equation modeling were performed to illustrate variations in the colloid transport profiles. These findings illustrate the importance of Si dissolution in the migration of metal-silicate colloids and highlight the significant influence of the heterogeneity of colloid size and composition on the transport/migration behavior of colloids in the environment.

Zooplankton grazing reduces the persistence of an anthropogenic pollution marker in lake water

AbstractWastewater treatment plant effluents release microbiological pollutants, including the intI1 gene (integrases of class 1 integron), which has been proposed as a target for monitoring anthropogenic pollution in surface waters. This gene correlates with antibiotic resistance genes, making it an important proxy for genetic contamination in aquatic environments. It is currently unclear whether intI1 found in lake water is mainly present due to continuous seeding or if autochthonous bacteria harbor this gene. To better understand the fate and dynamics of class 1 integrons in aquatic systems, we resorted to classical limnological monitoring of intI1 (qPCR) over multiple years in three different size fractions: free‐living bacteria, particle‐attached bacteria, and zooplankton‐attached bacteria. We also conducted experiments to elucidate the impact of grazers on the abundance of intI1. The monitoring of different size fractions of the Lake Maggiore microbial community showed a particle‐bound lifestyle for intI1‐hosting bacteria. Most of these bacteria originated from both a wastewater effluent that discharges into Lake Maggiore and the lake water itself (amplicon sequencing). We hypothesize that these bacteria grow on particles in open waters, making them particularly vulnerable to grazing by large filter feeders such as Daphnia. Therefore, the presence of Daphnia reduced the abundance of intI1 in lake water, whereas this was not true for other grazers such as Rotaria macrura or Poterioochromonas sp. Our study shows that the food web structure and temporal changes in the lake influence the abundance of intI1 and consequently the assessment of anthropogenic pollution.

Reclamation of coastal marshes to croplands shifts molecular composition of soil organic matter in the Bohai Rim

AbstractReclamation of coastal marshes to croplands lost massive reserves of soil organic matter (SOM). However, it is uncertain how reclamation shifts chemical composition of SOM and its size fractions. Here, we investigated this issue at two coastal marshes (Qilihai, degradation with seasonal waterlog; Beidagang, annual long‐term waterlog) along with adjacent long‐term reclaimed croplands on the coast of the Bohai Bay, China. Three SOM fractions: coarse particulate (cPOM, &gt;250 μm), fine particulate (fPOM, 53–250 μm), and mineral‐associated organic matter (&lt;53 μm) were analyzed by pyrolysis‐gas chromatography–mass spectrometry. Compared with the Qilihai marsh, the Beidagang marsh had higher SOM contents and lower proportions of microbial‐derived N‐containing compounds, which was attributed to the waterlogging‐induced weaker decomposition. Reclamation decreased the SOM contents (−53.6% to −63.3%) and proportions of lignin in cPOM (−44% to −52%) at both sites. However, reclamation‐induced shifts of molecular composition of SOM between the two sites diverged. At the Qilihai site, reclamation decreased the relative abundances of N‐containing compounds (−28%) but increased the proportions of lipids (+17%) and phenolics (+40.7%) in mineral‐associated organic matter. At the Beidagang site, reclamation decreased the proportions of monocyclic aromatics (−60.3% to −70.2%), but increased the relative abundances of N‐containing compounds (+175% to +407%) in all fractions. By contrast, the molecular composition of SOM at the two croplands had covergent pattern. In conclusion, despite of marsh locations with different waterlogging conditions, long‐term reclamation of wetlands to croplands generated convergent patterns in storage and molecular composition of SOM, which was attributed to the similar cropland cultivation.

A novel method to fabricate curved piezoelectric composites with high piezoelectric phase volume fraction

Underwater acoustic transducer based on high piezoelectric phase volume fraction, large thickness, large area and curved piezoelectric composites (PC) possess wider beam width, higher source level and better directivity. However, the curved PC with large size is difficult to fabricate. The preparation of curved PC with large size and high piezoelectric phase volume fraction is even more tricky. The reported methods have risks of depolarization, deformation and poor periodicity and are not suitable for preparing PC with high piezoelectric phase volume fraction and large thickness. In this work, we proposed a novel fabrication method of the shaped-mold-assisted dice and fill technology. The 1–3 PC with high piezoelectric ceramic volume fraction of 80 %, thickness of 4.3 mm and area of 340 mm × 77 mm and the 2-2 PC with high piezoelectric ceramic volume fraction of 89.5 %, thickness of 5 mm and area of 262 mm × 20 mm were fabricated. The piezoelectric coefficient d33 and relative constant stress permittivity ε33T/ε0 of 1–3 and 2-2 PCs were comparable to piezoelectric phase itself. In addition, both 1–3 and 2-2 PCs exhibited a favorable performance consistency. This method solved the problem of limited cutting depth of thin blade, realizing PC with the structure of high piezoelectric phase volume fraction (＞ 70 %) and large thickness (＞ 3 mm) and eliminates the risk of depolarization, deformation and poor periodicity. This work enabled the potential for achieving large area, high piezoelectric phase volume and special-shaped PCs, which offers new ideas for designing innovative acoustic transducers and facilitates the progress of acoustic sensing technology.

Particle-assisted formation of oil-in-liquid metal emulsions.

Gallium-based liquid metals (LM) have surface tension an order of magnitude higher than water and break up into micro-droplets when mixed with other liquids. In contrast, silicone oil readily mixes into LM foams to create oil-in-LM emulsions with oil inclusions. Previously, the LM was foamed through rapid mixing in air for an extended duration (over 2 hours). This process first results in the internalization of oxide flakes that form at the air-liquid interface. Once a critical fraction of these randomly shaped solid flakes is reached, air bubbles internalize into the LM to create foams that can internalize secondary liquids. Here, we introduce an alternative oil-in-LM emulsion fabrication method that relies on the prior addition of SiO2 micro-particles into the LM before mixing it with the silicone oil. This particle-assisted emulsion formation process provides a higher control over the composition of the LM-particle mixture before oil addition, which we employ to systematically study the impact of particle characteristics and content on the emulsions' composition and properties. We demonstrate that the solid particle size (0.8 µm to 5 µm) and volume fraction (1% to 10%) have a negligible impact on the internalization of the oil inclusions. The inclusions are mostly spherical with diameters of 20 to 100 µm diameter and are internalized by forming new, rather than filling old, geometrical features. We also study the impact of the particle characteristics on the two key properties related to the functional application of the LM emulsions in the thermal management of microelectronics. In particular, we measure the impact of particles and silicone oil on the emulsion's thermal conductivity and its ability to prevent deleterious gallium-induced corrosion and embrittlement of contacting metal substrates.&#xD.

Precision imaging of cardiac function and scar size in acute and chronic porcine myocardial infarction using ultrahigh-field MRI

Background7 T cardiac magnetic resonance imaging (MRI) studies may enable higher precision in clinical metrics like cardiac function, ventricular mass, and more. Higher precision may allow early detection of functional impairment and early evaluation of treatment responses in clinical practice and pre-clinical studies. Methods: Seven female German Landrace pigs were scanned prior to and at three time points (3–4 days, 7–10 days, and ~60 days) post myocardial infarction using a whole body 7 T system and three radiofrequency (RF) coils developed and built in-house to accompany animal growth. Results: The combination of dedicated RF hardware and 7 T MRI enables a longitudinal study in a pig model of acute and chronic infarction, providing consistent blood tissue contrast and high signal-to-noise ratio (SNR) in measurements of cardiac function, as well as low coefficients of variation (CoV) for ejection fraction (CoVintra-observer: 2%, CoVinter-observer: 3.8%) and infarct size (CoVintra-observer: 8.4%, CoVinter-observer: 3.8%), despite drastic animal growth. Conclusions: Best results are achieved via manual segmentation. We define state-of-the-art procedures for large animal studies at 7 T.

A size-dependent division strategy accounts for leukemia cell size heterogeneity

Heterogeneity in the size distribution of cancer cell populations is linked to drug resistance and invasiveness. However, understanding how such heterogeneity arises is still damped by the difficulties of monitoring the proliferation at the typical timescales of mammalian cells. Here, we show how to infer the growth regime and division strategy of leukemia cell populations using live cell fluorescence labeling and flow cytometry in combination with an analytical model where cell growth and division rates depend on powers of the size. We found that the dynamics of the size distribution of Jurkat T-cells is reproduced by (i) a sizer-like division strategy, with (ii) division times following an Erlang distribution and (iii) fluctuations up to ten percent of the inherited fraction of size at division. Overall, our apparatus can be extended to other cell types and environmental conditions allowing for a comprehensive characterization of the growth and division model different cells adopt.