Size-dependent Effects Research Articles

تأثيرات التكنولوجيا النانوية على الخواص الميكانيكية والفيزيائية للمعادن في أنظمة التبريد

Nanotechnology has revolutionized various scientific and engineering fields, including materials science. In this investigation, the focus is on examining the influence of titanium nanoparticle size on the mechanical and physical properties of metal. Incorporating nanoparticles into metal matrices presents an exciting opportunity to enhance material performance through size-dependent effects. Titanium nanoparticles have been chosen as the primary focus due to their unique properties and extensive applications. To assess the impact of nanoparticle size on mechanical properties, a series of experiments were conducted involving the production of metal composites with varying sizes of titanium nanoparticles. Mechanical properties, including yield strength and hardness, were measured using standard testing methods such as tensile testing and nanoindentation. The results indicate that smaller titanium nanoparticles contributed to improve mechanical properties, increased yield strength and hardness compared to the bulk metal. Additionally, the investigation explored the effects of titanium nanoparticle size on the physical properties of the metal composite. Thermal conductivity and coefficient of thermal expansion were measured to evaluate the thermal behavior of the composites. The findings demonstrate that the incorporation of titanium nanoparticles influences thermal conductivity and coefficient of thermal expansion, with variations that depend on nanoparticle size. Keywords: Nanotechnology, Titanium Nanoparticles, Mechanical Properties, Physical Properties, Metal Composites.

BYORP and Dissipation in Binary Asteroids: Lessons from DART

The near-Earth binary asteroid Didymos was the target of the planetary defense demonstration mission DART in 2022 September. The smaller binary component, Dimorphos, was impacted by the spacecraft in order to measure momentum transfer in kinetic impacts into rubble piles. DART and associated Earth-based observation campaigns have provided a wealth of scientific data on the Didymos–Dimorphos binary. DART revealed the largely oblate and ellipsoidal shape of Dimorphos before the impact, while the postimpact observations suggest that Dimorphos now has a prolate shape. Here we add those data points to the known properties of small binary asteroids and propose new paradigms of the radiative binary Yarkovsky–O’Keefe–Radzievskii–Paddack (BYORP) effect as well as tidal dissipation in small binaries. We find that relatively spheroidal bodies like Dimorphos made of small debris may experience a weaker and more size-dependent BYORP effect than previously thought. This could explain the observed values of period drift in several well-characterized binaries. We also propose that energy dissipation in small binaries is dominated by relatively brief episodes of large-scale movement of (likely surface) materials, rather than long-term steady-state tidal dissipation. We propose that one such episode was triggered on Dimorphos by the DART impact. Depending on the longevity of this high-dissipation regime, it is possible that Dimorphos will be more dynamically relaxed in time for the Hera mission than it was in the weeks following the impact.

Silicon dioxide nanoparticles induce anxiety-like behavior in a size-specific manner via the microbiota-gut-brain axis

The impacts of silicon dioxide nanoparticles (SiO2-NPs) on human health have attracted increasing interest due to their widespread utilization in medicine and food additives. However, the size-dependent effects of SiO2-NPs on brain health remain sparse. Herein we investigated alterations in behavioral patterns, the gut microbiota, inflammation and oxidative stress of mice after a 12-week exposure to SiO2-NPs with either small size (NP-S) or large size (NP-L). A more pronounced deleterious effect of NP-S was found on anxiety-like behavior in mice relative to NP-L. We also found that SiO2-NPs exposure induced inflammation and oxidative stress in the colon, hippocampus and cortex of mice in a size-specific manner. Correlation network analysis revealed potential links between anxiety-like behavior and SiO2-NPs-induced shifts in the gut microbiota including Parvibacter, Faecalibaculum, Gordonibacter and Ileibacterium. Furthermore, anxiety-like behavior caused by SiO2-NPs exposure exhibited correlations with decreased levels of hippocampal IL-10 and cortex Nqo1 as well as increased levels of intestinal Acox1 and hippocampal TNF-α. Therefore, our findings suggest that exposure to SiO2-NPs promoted anxiety-like behavior through the mediation of interplay between the gut and the brain, and SiO2-NPs of smaller size may generate a more adverse effect on brain health.

Effects of polystyrene microspheres on the swimming behavior and metabolism of grass carp (Ctenopharyngodon idella)

Microplastics (MPs) are a heterogeneous class of pollutants fouling aquatic environments and they are hazardous to aquatic organisms. This study investigated the size-dependent effects of polystyrene microspheres (PSMPs) on the swimming ability, metabolism, and oxidative stress of juvenile grass carp (Ctenopharyngodon idella). Test fish were exposed to four sizes of PSMPs (0.07, 0.5, 5, and 20-μm), and swimming ability was tested after different exposure times (2, 7, and 15 days). To measure the effect on swimming ability, critical swimming speed (Ucrit) was determined, and to assess metabolic effects, oxygen consumption (MO2), routine metabolic rate (RMR), maximum oxygen consumption (MMR), and excess post-exercise oxygen consumption (EPOC) were determined. To assess the effects on oxidative stress, the activities of two antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT) were determined in the liver and gills of test fish. After exposure to 20 μm PSMPs, there was a significant drop in Ucrit compared to the control group (P<0.05), with decreases of 22 % on Day 2 and Day 7, and 21 % on Day 15. The RMR and MMR increased significantly (P<0.05), the RMR by 23.9 % on Day 2 and the MMR by 17.2 % on Day 2 and on Day 15, 44.7 % and 20.0 % respectively. The EPOC decreased with exposure time, by 31 % (0.07-μm), 45 %-(0.5-μm), 49 % (5-μm), and 57 % (20-μm) after 15 days. Exposure to the larger PSMPs increased CAT and SOD activity more than the smaller PSMPs and the increases began with SOD activity in the gills. The larger PSMPs were consistently more harmful to juvenile grass carp than the smaller PSMPs. Our results clearly show that PSMPs have detrimental effects on juvenile grass carp and provide additional scientific evidence that environmental monitoring and regulation of microplastic pollution is necessary.

Generalized thermoelastic damping in micro/nano-ring resonators undergoing out-of-plane vibration

Micro/nano-ring or ring-based resonators are popular core components of sensors and actuators. Accurately predicting the quality factor (Q) based on thermoelastic damping (TED) is intensively crucial for designing high-performance micro/nano-ring resonators. In this work, adopting the modified-couple-stress (MCS) and nonlocal-dual-phase-lagging (NDPL) models, analytical TED expressions are developed for circular cross-section micro/nano-rings undergoing out-of-plane vibration for the first time. Multiple effects are considered in the modeling procedure, which are size-dependence effects in the mechanical and thermal fields, the non-Fourier effect of heat conduction, and three-dimensional temperature gradients in the circumferential, radial, and tangential directions. Therefore, the proposed TED models are more comprehensive compared to existing TED models. The coefficients (CC and CM) associated with the out-of-plane vibration mode are examined from an energy-ratio perspective. Moreover, the investigation is focused on the impacts of the MCS and NDPL non-Fourier effects on the fluctuation temperature and TED spectra. The results indicate as CC and CM approach one, the bending energy emerges as the predominant component of the stored energy. Notably, the NDPL non-Fourier effect significantly affects the fluctuation temperature within the ring operated in high-order modes. In special, the distributions of fluctuation temperature in the circumferential direction exhibit periodic patterns that are closely correlated with the modal order. Furthermore, TED can be suppressed by the MCS size-dependence effect. Meanwhile, the behavior of TED at high frequencies is simultaneously determined by the dimension of heat conduction, phase-lagging times, and nonlocal thermal-length parameter.

Size-Dependent Effect of Titania Nanotubes on Endoplasmic Reticulum Stress to Re-establish Diabetic Macrophages Homeostasis.

In patients with diabetes, endoplasmic reticulum stress (ERS) is a crucial disrupting factor of macrophage homeostasis surrounding implants, which remains an obstacle to oral implantation success. Notably, the ERS might be modulated by the implant surface morphology. Titania nanotubes (TNTs) may enhance diabetic osseointegration. However, a consensus has not been achieved regarding the tube-size-dependent effect and the underlying mechanism of TNTs on diabetic macrophage ERS. We manufactured TNTs with small (30 nm) and large diameters (100 nm). Next, we assessed how the different titanium surfaces affected diabetic macrophages and regulated ERS and Ca2+ homeostasis. TNTs alleviated the inflammatory response, oxidative stress, and ERS in diabetic macrophages. Furthermore, TNT30 was superior to TNT100. Inhibiting ERS abolished the positive effect of TNT30. Mechanistically, topography-induced extracellular Ca2+ influx might mitigate excessive ERS in macrophages by alleviating ER Ca2+ depletion and IP3R activation. Furthermore, TNT30 attenuated the peri-implant inflammatory response and promoted osseointegration in diabetic rats. TNTs with small nanodiameters attenuated ERS and re-established diabetic macrophage hemostasis by inhibiting IP3R-induced ER Ca2+ depletion.

Computational Linear and Nonlinear Free Vibration Analyses of Micro/Nanoscale Composite Plate-Type Structures With/Without Considering Size Dependency Effect: A Comprehensive Review

Abstract Recently, the mechanical performance of various mechanical, electrical, and civil structures, including static and dynamic analysis, has been widely studied. Due to the neuroma's advanced technology in various engineering fields and applications, developing small-size structures has become highly demanded for several structural geometries. One of the most important is the nano/micro-plate structure. However, the essential nature of highly lightweight material with extraordinary mechanical, electrical, physical, and material characterizations makes researchers more interested in developing composite/laminated-composite-plate structures. To comprehend the dynamical behavior, precisely the linear/nonlinear-free vibrational responses, and to represent the enhancement of several parameters such as nonlocal, geometry, boundary condition parameters, etc., on the free vibrational performance at nano/micro scale size, it is revealed that to employ all various parameters into various mathematical equations and to solve the defined governing equations by analytical, numerical, high order, and mixed solutions. Thus, the presented literature review is considered the first work focused on investigating the linear/nonlinear free vibrational behavior of plates on a small scale and the impact of various parameters on both dimensional/dimensionless natural/fundamental frequency and Eigen-value. The literature is classified based on solution type and with/without considering the size dependency effect. As a key finding, most research in the literature implemented analytical or numerical solutions. The drawback of classical plate theory can be overcome by utilizing and developing the elasticity theories. The nonlocality, weight fraction of porosity, or the reinforcements, and its distribution type of elastic foundation significantly influence the frequencies.

Body-size-dependent effects of landscape-level resource energetics on pollinator abundance in woodland remnants.

Land use change alters floral resource availability, thereby contributing to declines in important pollinators. However, the severity of land use impact varies by species, influenced by factors such as dispersal ability and resource specialization, both of which can correlate with body size. Here. we test whether floral resource availability in the surrounding landscape (the 'matrix') influences bee species' abundance in isolated remnant woodlands, and whether this effect varies with body size. We sampled quantitative flower-visitation networks within woodland remnants and quantified floral energy resources (nectar and pollen calories) available to each bee species both within the woodland and the matrix. Bee abundance in woodland increased with floral energy resources in the surrounding matrix, with strongest effects on larger-bodied species. Our findings suggest important but size-dependent effects of declining matrix floral resources on the persistence of bees in remnant woodlands, highlighting the need to incorporate landscape-level floral resources in conservation planning for pollinators in threatened natural habitats.

Hygrothermomechanical loading-induced vibration study of multilayer piezoelectric nanoplates with functionally graded porous cores resting on a variable viscoelastic substrate

Harnessing vibrations in multifunctional nanostructured plates is pivotal to next-gen microsystems but necessitates understanding scale effects under multifield loading. Investigated in this work are the forced and unforced vibrations of multilayered piezoelectric nanoplates supported on a variable viscoelastic medium and loaded hygrothermo-electromechanically with functionally graded porous (FGP) cores. The viscoelastic foundation is supposed to demonstrate non-linear changes in both stiffness and damping characteristics in the x-coordinate direction. The goal is to improve the accuracy of the results by using the nonlocal strain gradient theory (NSGT) and the third-order shear deformation assumption (TSDA), which include the effects of hardening and softening materials. The FGP core layer considers four states of porosity distribution patterns. These porosity distributions are expected to change in both the in-plane and thickness directions. The governing partial differential equations resulting from Hamilton's principle can be reduced to a system of algebraic equations by applying the Galerkin method. The parametric studies evaluate the effects of several factors, such as the initial electric voltage, viscoelastic medium parameters, moisture rise, temperature changes, porosity distributions, FG index, nonlocal and strain gradient features, and boundary conditions, on the vibration response. The novelty lies in the incorporation of advanced theories, such as the NSGT and TSDT, which capture size-dependent effects and material hardening/softening phenomena. Additionally, the consideration of four distinct porosity distribution patterns in the FGP core layer provides insights into the influence of porosity gradients on the dynamic response. The proposed model can guide the design and optimization of multifunctional nanostructured plates for applications in next-generation microsystems, energy harvesting devices, and smart structures.

The size-dependent effects of nanoplastics in mouse primary hepatocytes from cells to molecules

Nanoplastics (NPs) are easily ingested by organisms and their major accumulation organ was determined to be liver. To date, the size-dependent cytotoxicity of NPs on mammalian hepatocytes remains unclear. This study utilized mouse primary hepatocytes and catalase (CAT) as specific receptors to investigate the toxicity of NPs from cells to molecules, focusing on size-dependent effects. Results showed that the larger the particle size of NP at low doses (≤50 mg/L), the most pronounced inhibitory effect on hepatocyte viability. 20 nm NPs significantly inhibit cell viability only at high doses (100 mg/L). Larger NP particles (500 nm and 1000 nm) resulted in a massive release of lactate dehydrogenase (LDH) from the cell (cell membrane damage). Reactive oxygen species (ROS), superoxide dismutase (SOD) and CAT tests suggest that NPs disturbed the cellular antioxidant system. 20 nm NPs show great strength in oxidizing lipids and disrupting mitochondrial function compared to NPs of other particle sizes. The degree of inhibition of CAT activity by different sized NPs was coherent at the cellular and molecular levels, and NP-500 had the most impact. This suggests that the structure and microenvironment of the polypeptide chain in the vicinity of the CAT active site is more susceptible to proximity and alteration by NP-500. In addition, the smaller NPs are capable of inducing relaxation of CAT backbone, disruption of H-bonding and reduction of α-helix content, whereas the larger NPs cause contraction of CAT backbone and increase in α-helix content. All NPs induce CAT fluorescence sensitization and make the chromophore microenvironment hydrophobic. This study provides new insights for NP risk assessment and applications.

Nonlinear Energy Harvesting From a Base Excited Size-Dependent Flexoelectric Nanostructure with Off-Center Rigid Tip Mass Subjected to Axial Pulsating Excitation

This study explores the incorporation of flexoelectricity and size-dependence effects in electricity harvesting from a novel nanostructure. This nanostructure, featuring a cylindrical-shaped rigid tip mass, is stimulated at the base with axial pulsating excitation. Designed with a nanobeam material, the energy harvester accounts for structural nonlinearity, flexoelectricity, and the presence of an off-center rigid mass, operating under multiple excitations. The eigenanalysis is employed to delve into the dynamic characteristics of the system, providing engineers with insights to identify safe operating regions and operational constraints. Additionally, perturbation techniques are utilized to estimate steady-state voltage and power characteristics, with a focus on maximizing voltage and power generation. The study underscores the impact of size dependence on nanoscale design, flexoelectricity, asymmetric tip mass, and various excitation parameters within a constrained operating range. Analysis reveals how saddle-node and pitchfork bifurcations can disrupt energy harvesting performance and suggests strategies for mitigating these issues by adjusting operational parameters. Moreover, the study emphasizes the significant role of the off-center rigid tip mass in energy estimation compared to conventional microstructure-based energy harvesters. Analytical findings are rigorously validated against numerical results under different resonant scenarios, showcasing the accuracy of the analytical approach in capturing nonlinear sources and optimizing energy harvesting from nanostructures.

Size-dependent effects of microplastics on intestinal microbiome for Perna viridis

Microplastics pollution threatens to marine organisms, particularly bivalves that actively ingest and accumulate microplastics of certain sizes, potentially disrupting intestinal homeostasis. This study investigated the microplastic abundance in wild and farmed mussels around Singapore, and examined the size-dependent effects of nano- to micro-scale polystyrene (0.5 µm/5 µm/50 µm) on the mussel intestinal microbiome in the laboratory. The field investigation revealed higher microplastic abundance in farmed mussels compared to wild ones. Experimentally, mussels exposed to 0.6 mg/L of microplastics for 7 days, followed by a 7-day depuration period, showed substantial impacts on Spirochaetes and Proteobacteria, facilitating the proliferation of pathogenic species and differentially affecting their pathogenic contributions. Metagenomics analysis revealed that microplastic exposure reduced Spirochaeta's contribution to virulence and pathogenicity loss, did not affect Vibrio and Oceanispirochaeta's pathogenicity, and increased Treponema and Oceanispirochaeta's contributions to pathogenicity loss. Moreover, microplastics increased transmembrane transporters and impacted oxidative phosphorylation enzymes, impairing energy metabolism. These effects persisted after depuration, indicating lack of resilience in the microbiome. Nano- and micro-scale plastics perturbed the mussel microbiome composition and functions in a size-dependent manner, with nano-plastics being the most disruptive. The increasing use and sale of aquaculture equipment of plastic may exacerbate the intestinal dysbiosis in bivalves, which threatens consumers' health.

Size-dependent effect on the interaction of surface waves in micropolar thermoelastic medium with dual pore connectivity

This research tackles a critical knowledge gap in Rayleigh surface wave propagation. It offers a comprehensive analysis that surpasses previous limitations. A size-dependent micropolar medium with unique void distributions and thermal effects is considered in this work. The constitutive relations and equations of motion for a nonlocal micropolar thermoelastic medium with double voids (MTMWDV) have been established by using Eringen’s nonlocal elasticity theory. Employing the three-phase-lag thermoelasticity theory (TPLTE), the study utilizes a wave-mode method to derive analytical solutions for Rayleigh waves in a nonlocal MTMWDV. To gain a comprehensive understanding of wave behavior, we solve the characteristic equation and analyze its roots, applying a filter based on the surface wave decay condition. A medium with stress-free and isothermal boundaries is explored through computational simulations to determine the attenuation coefficient and phase velocity. Furthermore, particle motion analysis is conducted to complement the analytical and computational approaches. Moreover, the influence of the nonlocal parameter and various thermoelastic models on these wave phenomena is investigated. The validity of the current mathematical model is confirmed through the derivation of particular scenarios.

Unveiling the molecular mechanisms of size-dependent effect of polystyrene micro/nano-plastics on Chlamydomonas reinhardtii through proteomic profiling

Microplastics have become a prevalent environmental pollutant due to widespread release and production. Algae, as primary producers, play a crucial role in maintaining the ecological balance of freshwater environments. Despite reports on the inhibition of microalgae by microplastics, the size-dependent effects on microalgae and associated molecular mechanism remain poorly understood. This study investigates the impacts of three polystyrene micro/nano-plastics (PS-MNPs) with different sizes (100 nm, 350 nm, and 6 μm) and concentrations (25–200 mg/L) on Chlamydomonas reinhardtii (C. reinhardtii) throughout its growth period. Results reveal size- and concentration-dependent growth inhibition and induction of oxidative stress by PS-MNPs, with microalgae exhibiting increased vulnerability to smaller-sized and higher-concentration PS-MNPs. Proteomics analysis elucidates the size-dependent suppression of proteins involved in the photosynthesis process by PS-MNPs. Photosynthetic activity assays demonstrate that smaller PS-MNPs more significantly reduce chlorophyll content and the maximal photochemical efficiency of photosystem II. Finally, electron microscope and Western blot assays collectively confirm the size effect of PS-MNPs on microalgae growth is attributable to suppressed protein expression rather than shading effects. This study contributes to advancing our understanding of the intricate interactions between micro/nano-plastics and algae at the molecular level, emphasizing the efficacy of proteomics in dissecting the mechanistic aspects of microplastics-induced biological effects on environmental indicator organisms.

Functional gradation of material coefficients and size –effects in heat conduction: Numerical simulations

It is well known that the classical theory of heat conduction is scale invariant. On the other hand, it is experimentally evident that size-dependent effects are observable in small samples of micro/nano-scale dimensions. Incorporation of higher-order gradients of primary field variables into constitutive relationships yields a qualitative explanation of size-effects. Form the mathematical point of view, the governing equations are given by the partial differential equations (PDE) with higher-order derivatives in higher-grade continuum theories. As compared with classical theory of continua, the other complication of governing equations occurs in case of continuous media with functional gradation of material coefficients, when the problem is described by the PDE with variable coefficients. The traditional weak formulations are considered in global sense, hence the whole analysed domain/boundary is to be discretized into finite size elements. On the other hand, the strong formulations bring better computational efficiency because of elimination of integrations, but the price which should be paid is the need to approximate higher order derivatives of field variables. One of the most criticized shortcoming of the finite element (FE) approximation is its limited continuity on element intersections. The C0 continuity is insufficient for calculation of gradients of field variables on element intersections as well as for numerical solution of problems with governing equations of higher than 2nd order partial differential equations (PDE). Recently widely spread and elaborated mesh-free approximations utilize the higher order continuous shape functions. Another advantage is elimination of discretization of the analysed domain into finite elements, since only the nodes are scattered on the domain and its boundary. Both the strong and weak formulations are applicable in combination with mesh-free approximations. The Moving Finite Element Approximation (MFEA) and its utilization in mesh-free formulations for heat conduction problems is presented in this paper.

Size-dependent deleterious effects of nano- and microplastics on sperm motility

IntroductionGrowing concerns regarding the reproductive toxicity associated with daily life exposure to micro-/nano-plastics (abbreviated as MNPs) have become increasingly prevalent. In reality, MNPs exposure involves a heterogeneous mixture of MNPs of different sizes rather than a single size. MethodsIn this study, an oral exposure mouse model was used to evaluate the effects of MNPs of four size ranges: 25–30 nm, 1–5 µm, 20–27 µm, and 125–150 µm. Adult male C57BL/6 J mice were administered environmentally relevant concentrations of 0.1 mg MNPs/day for 21 days. After that, open field test and computer assisted sperm assessment (CASA) were conducted. Immunohistochemical analyses of organ and cell type localization of MNPs were evaluated. Testicular transcriptome analysis was carried out to understand the molecular mechanisms. ResultsOur result showed that MNPs of different size ranges all impaired sperm motility, with a decrease in progressive sperm motility, linearity and straight-line velocity of sperm movement. Alterations did not manifest in animal locomotion, body weight, or sperm count. Noteworthy effects were most pronounced in the smaller MNPs size ranges (25–30 nm and 1–5 µm). Linear regression analysis substantiated a negative correlation between the size of MNPs and sperm curvilinear activity. Immunohistochemical analysis unveiled the intrusions of 1–5 µm MNPs, but not 20–27 µm and 125–150 µm MNPs, into Leydig cells and testicular macrophages. Further testicular transcriptomic analysis revealed perturbations in pathways related to spermatogenesis, oxidative stress, and inflammation. Particularly within the 1–5 µm MNPs group, a heightened perturbation in pathways linked to spermatogenesis and oxidative stress was observed. ConclusionsOur data support the size-dependent impairment of MNPs on sperm functionality, underscoring the pressing need for apprehensions about and interventions against the escalation of environmental micro-/nano-plastics contamination. This urgency is especially pertinent to small-sized MNPs.

Nonlinear dynamics of contact interaction porous size-dependent Euler-Bernoulli beams resonators with clearance: Numerical analysis of the stability problem

In this study, a new mathematical model is developed to study the contact interactions of nano- and micro-electro-mechanical (NEMS/MEMS) beam resonators. The structural elements are considered as porous, size-dependent Euler-Bernoulli beams subjected to a variable transverse load. The beams resonators are located one above the other with minimal clearance. The analysis includes interactions between beams of both linear elastic and physically nonlinear materials, following the approach of Professor B. Ya. Kantor. Modified Coupled Stress Theory (MCST) is used to account for size-dependent effects. Hamilton's principle is applied to derive new size-dependent equations of motion together with the corresponding boundary and initial conditions. This study presents a new approach to the analysis of the chaotic dynamics of the contact interaction of porous, size-dependent Euler-Bernoulli beams. They are considered as systems with an "almost" infinite number of degrees of freedom. This innovative approach uses Principal Component Analysis (PCA) and Wavelet Analysis to filter out noise from signals. In addition, different beam interaction scenarios are classified including static Euler and Rayleigh instabilities as well as interactions between Koning-Taub and Richtmeyer-Meshkov instabilities. A significant discovery of this investigation is the chaotic behaviour of nonlinear vibrations during the contact interaction of these closely positioned beams. This demonstrates the complex dynamics involved in these high technology mechanical systems.

Size-dependent Effect on Foliar Utilization and Biocontrol Efficacy of Emamectin Benzoate Delivery Systems.

The development of nanopesticides provides new avenues for pesticide reduction and efficiency improvement. However, the size effect of nanopesticides remains unclear, and its underlying mechanisms of influence have become a major obstacle in the design and application of pesticide nanoformulations. In this research, the noncarrier-coated emamectin benzoate (EB) solid dispersions (Micro-EB and Nano-EB) were produced under a constant surfactant-to-active ingredient ratio by a self-emulsifying-carrier solidification technique. The particle size of Micro-EB was 162 times that of spherical Nano-EB. The small size and large specific surface area of Nano-EB facilitated the adsorption of surfactants on the surface of the particles, thereby improving its dispersibility, suspensibility, and stability. The pinning effect of nanoparticles significantly suppressed droplet retraction and rebounding. Moreover, Nano-EB exhibited a 25% higher retention of the active ingredient on cabbage leaves and a 70% higher washing resistance than Micro-EB, and both were significantly different. The improvement of abilities in wetting, spreading, and retention of Nano-EB on crop leaves contributed to the increase in foliar utilization, which further resulted in a 1.6-fold enhancement of bioactivity against target Spodoptera exigua compared to Micro-EB. Especially, Nano-EB did not exacerbate the safety risk to the nontarget organism zebrafish with no significant difference. This study elaborates the size effect on the effectiveness and safety of pesticide formulations and lays a theoretical foundation for the development and rational utilization of efficient and environmentally friendly nanopesticides.

A molecular switch in RCK2 triggers sodium-dependent activation of KNa1.1 (KCNT1) potassium channels

The Na+-activated K+ channel KNa1.1, encoded by the KCNT1 gene, is an important regulator of neuronal excitability. How intracellular Na+ ions bind and increase channel activity is not well understood. Analysis of KNa1.1 channel structures indicate that there is a large twisting of the βN-αQ loop in the intracellular RCK2 domain between the inactive and Na+-activated conformations, with a lysine (K885, human subunit numbering) close enough to potentially form a salt bridge with an aspartate (D839) in βL in the Na+-activated state. Concurrently, an aspartate (D884) adjacent in the same loop adopts a position within a pocket formed by the βO strand. In carrying out mutagenesis and electrophysiology with human KNa1.1, we found that alanine substitution of selected residues in these regions resulted in almost negligible currents in the presence of up to 40 mM intracellular Na+. The exception was D884A, which resulted in constitutively active channels in both the presence and absence of intracellular Na+. Further mutagenesis of this site revealed an amino acid size-dependent effect. Substitutions at this site by an amino acid smaller than aspartate (D884V) also yielded constitutively active KNa1.1, and D884I had Na+ dependence similar to wild-type KNa1.1, while increasing the side-chain size larger than aspartate (D884E or D884F) yielded channels that could not be activated by up to 40 mM intracellular Na+. We conclude that Na+ binding results in a conformational change that accommodates D884 in the βO pocket, which triggers further conformational changes in the RCK domains and channel activation.

Size dependent ingestion and effects of microplastics on survivability, hematology and intestinal histopathology of juvenile striped catfish (Pangasianodon hypophthalmus)

Microplastic pollution is drastically increasing in aquatic ecosystems and it is assumed that different sizes of microplastics have diverse impacts on the physiology of aquatic organisms. Therefore, this study was intended to examine the ingestion and size specific effects of polyamide microplastic (PA-MP) on different physiological aspects such as growth, feed utilization, survivability, blood parameters and intestinal histopathology of juvenile striped catfish (Pangasianodon hypophthalmus). In a 28-day exposure, the fish were fed with different sized PA-MP with a concentration of 500 mg per kg of feed in order to simulate highly microplastic contaminated environment. Three different treatments were set for this experiment i.e. T1, 25–50 μm (smaller microplastic); T2, 300 μm-2 mm (larger microplastic); T3, (mixed) including a control (C); each had three replicates. The highest ingestion was recorded in the gastrointestinal tract (GIT) of fish exposed to smaller PA-MP treatments (T1 followed by T3). The results also showed compromised weight gain (WG; g), specific growth rate (SGR; %/day) and feed conversion ratio (FCR) with the exposure of PA-MP. Besides, survivability significantly reduced among treatments with the ingestion of smaller sized microplastic and found lowest in T1 (65.0 ± 5.0). In addition, the presence of PA-MP in feed negatively affected the concentration of hemoglobin and blood glucose. Similarly, smaller PA-MP caused most erythrocytic cellular and nuclear abnormalities; found highest in T1 that significantly different from other treatments (p < 0.05). Various histopathological deformities were observed in fish fed with PA-MP incorporated feed. The principal component analysis (PCA) showed that the toxicity and stress imparted by smaller PA-MP affected the survivability and blood parameters where larger PA-MP caused mild to severe abnormalities. Based on eigenvector values, the major abnormalities in intestine included occurrence of epithelium columnar degeneration (ECD: 0.402; PC1), hyperplasia of internal mucosa (HISM: 0.411; PC1), beheading of villi (BV: 0.323; PC1), atrophy of mucosa (AM: 0.322; PC1), tiny vacuoles in apical villi (TV: 0.438. PC2), crypt degeneration (CD: 0.375: PC2) and atrophy of goblet cell (AGC: 0.375; PC2). Therefore, it has been speculated that the size based PA-MP ingestion in the GIT interfered with the digestion and absorption as well as caused deformities that reflected negatively in survivability and hemato-biochemical parameters of juvenile striped catfish.