Research Site Research Articles

Spatial Characteristics of Mithila Architecture

Mithila, a region steeped in history and culture, nestled within the Indian subcontinent, emerges as a captivating tapestry of geographical and architectural heritage. Bounded by significant natural landmarks, Mithila's rich history dates back to 6000 BCE, enduring diverse epochs under various rulers. In the face of relentless urbanization, the study of Mithila's architecture becomes a crucial endeavor to conserve the essence of this community. This research explores the intricate layers of Mithila's architectural legacy, delving into the living history of its people and providing a roadmap for integrating tradition into contemporary developments. Through a comprehensive literature review, the study navigates cultural and historical transitions, architectural intricacies, and traditional residential structures in Mithila. The research methodology employs qualitative methods, including participant observation and semi-structured interviews, focusing on Janakpur as a research site. Analyzing spatial characteristics reveals intentional orientations, meticulous planning, and the ingenious use of bamboo, mud plaster, and vibrant paintings in building materials and technology. The elevation treatment of Mithila houses emerges as a dynamic manifestation of cultural identity seamlessly blended with architectural innovation. This research contributes to the discourse on cultural and heritage conservation, emphasizing the urgency of empowering local communities to preserve and celebrate Mithila's unique architectural legacy.

The Effectiveness of Birthing Ball Gymnastics on Primigravida Maternity Gestational Age

One of the riskiest pregnancy conditions is post-term pregnancy, which is defined as a gestational age greater than the estimated day of delivery (HPL). This is because difficulties may arise for both the mother and the fetus, particularly when birthing is imminent. Birthing ball gymnastics is one complementary therapy that can help labor commence at full gestational age (term). The aim of the research was to evaluate the impact of birthing ball gymnastics on the gestational age of moms who were primigravidae. The study's quasi-experimental design, which included a treatment group and a control group, was randomized post-test only. The Midwife Independent Practice Place (TPMB) Sidoarjo served as the research site for five months, starting in February 2023 and ending in June 2023. Purposive sampling procedures were used in the study to collect samples from participants who satisfied the inclusion criteria. This resulted in the collection of 30 samples, 15 of which were treatment groups and 15 of which were control groups. Birthing ball exercises and birthing ball gymnastics are provided twice a week for 20 minutes to the treatment group starting at 32 weeks gestation. The control group, on the other hand, had no birthing ball gymnastics instruction and was assessed right away. Utilizing the chi square statistical test to analyze the data, the study's results (p value (0.000) < 0.05) indicated that birthing ball gymnastics was effective in reducing primigravida maternity mothers' gestational age at the Midwife Independent Practice Place (TPMB) in Sidoarjo. In order to prevent the delivery process with post-term gestational age, it is envisaged that the installation of birthing ball gymnastics can be carried out in healthcare facilities such as hospitals, clinics, or Midwife Independent Practice Places (TPMD) for primigravida pregnant women.

Genomic and Spatial Analysis on the Recent Transmission of Mycobacterium tuberculosis in Eastern China: A 10-Year Retrospective Population-Based Study.

Understanding the mode of Mycobacterium tuberculosis (M. tuberculosis) transmission is crucial for disease prevention and control. Compared to traditional genotyping methods, whole genome sequencing (WGS) provides higher resolution and comprehensive genetic information, enabling the tracing of infection sources and determining of transmission routes to resolve extensive tuberculosis (TB) outbreaks. We conducted a ten-year study on the transmission of M. tuberculosis in a population in eastern China. We selected Lianyungang, an eastern city in China, as the study site. Patients diagnosed with active pulmonary TB from 2011 to 2020 were enrolled as the study subjects. We isolated and sequenced 2252 M. tuberculosis. Strains with pairwise genetic distances of less than 12 single nucleotide polymorphisms were defined as genomic clusters and which were considered recent transmissions. Kernel density estimation and K-function analysis were applied to explore the spatial distribution of recently transmitted strains. After excluding non-tuberculous mycobacteria and duplicated samples, 2114 strains were included in the final analysis. These strains comprised lineage 2 (1593, 75.35%) and 4 (521, 24.65%). There were 672 clustered strains, with a recent transmission rate of 31.79%. The logistic regression model showed that the risk of recent transmission was high in students [adjusted odds ratio (aOR): 2.68, 95% confidence interval (CI): 1.63-4.49, P<0.001] and people infected with L2.2.1 strains (aOR: 1.59, 95% CI: 1.20-2.12). Higher spatial aggregation of TB transmission has been concentrated in Haizhou, Donghai, and Guanyun for the past 10 years. Three outbreaks affecting 46 patients were spatially spaced, with 11 to 23 persons each. Different groups exhibited varying geographic distances between the initial and later cases. There are areas with a high risk of transmission for M. tuberculosis in the research site, and the risk varies among different populations. Accurate prevention strategies targeted at specific regions and key populations can help curb the prevalence of TB.

5Es Learning Model of Constructivism and The Classroom Environment for Critical Thinking in Nigeria

Constructivism is a concept that has been widely embraced by many teachers over the years, in many countries of the world. However, the meanings that are attached to this term and the understanding and compliance with the principles and requirements for appropriate classroom learning environment are varied and often inadequately understood. Teachers need to have a sound understanding of what constructivism learning environment means and its requirements for the appropriate classroom learning environment particularly as prescribed by the 5Es model of the theory to prepare their classroom settings; lesson objectives and to appropriately teach and evaluate their learners by employing its tenets and principles knowledgeably and effectively. This paper explicates some of the theoretical background of constructivism in accordance with the principles of 5Es model and then presents a detailed study and description of classroom learning environment in Nigeria in which a traditional classroom lesson and a constructivist environment are described and analyzed. The study was descriptive qualitive design using observation as the instrument of data collection from five purposefully selected schools (research sites). Fieldnotes were developed from the observation and thematically analysed. The learning environments were found to have grossly fall short of the requirements of 5Es model of constructivism learning environment particularly for promoting critical thinking.

Disparate trajectories of cognitive aging among American Indian and Alaskan Native people with and without HIV.

This study describes trajectories of cognitive aging among American Indian/Alaskan Native (AI/AN) adults with and without HIV and the role of immunosenescence longitudinally. We characterized trajectories of cognitive aging in a sample of 333 AI/AN and 309 non-Hispanic White (NHW) adults who were followed longitudinally for up to 20 years by the HIV Neurobehavioral Research Program (HNRP) across six U.S. research sites. We used growth curve modeling with autoregressive Lag-1 structures and heterogeneous residual variances to assess the role of ethnoracial identity and HIV grouping upon decline in trajectories of cognitive aging. HIV- AI/AN adults demonstrated earlier and steeper decline in normative trajectories of cognitive aging on tasks of processing speed, timed tasks of attention/working memory, executive function, and psychomotor speed in comparison to HIV- NHW adults. Accentuated trajectories of cognitive aging were evident in both HIV+ and HIV+ immunosuppressed groups in comparison to HIV- peers and were primarily driven by the role of immunosenescence. AI/AN disparities in trajectories of cognitive aging are evident and are likely explained by the interplay of biopsychosociocultural factors, including immunosenescence. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Effects of Rainfall Intensity, Kinetic Energy and Slope Angle to the Upslope, Downslope, and Lateral Slope Components of Splash Erosion in Hillslope Agriculture: A Case in Badiangan, Ajuy, Iloilo

This study was conducted in Barangay Badiangan, Ajuy, Iloilo City, Philippines (11°10’N, 122°58’E) to determine the effects of rainfall intensity and other rainfall-derived parameters on the directional components of splash erosion in hillslopes. There are five experimental set-ups with slope angles ranging from 0% to 48% were tested under natural rainfall conditions using a modified splash collector. The data collected shows that kinetic energy, slope, and rainfall intensity have shown significant effects on splash erosion. The models obtained using regression analysis are 𝑄𝑄𝑑𝑑𝑑𝑑𝑑𝑑=0.0093(𝐾𝐾𝐾𝐾0.80) and 𝑄𝑄𝑡 𝑡𝑡𝑡𝑡𝑡𝑡𝑡𝑡=0.060(𝐾𝐾𝐾𝐾0.107)(𝑆𝑆0.700)(𝐼𝐼200.700) . The model equation performance has been validated using the Standard Error of Estimates with values of 12 and 9.4 for splash detachment and splash transport, respectively. The constants used for kinetic energy in detachment and slope in transport align with the research by Quansah (1981) for sandy soil, which is similar (the characteristics) to the soil at our research site. Additionally, rainfall intensity, especially with a 20-min duration, generated the best model as it yielded the lowest SEE value for all cases.

Penstock pipe’s hydraulic design for the mini hydropower plant at Besai Kemu, Bukit Kemuning, Lampung, Indonesia

Abstract Hydropower, as a renewable energy source, holds significant potential for electricity generation. However, optimizing the design of hydropower penstocks to ensure efficient energy conversion remains a complex challenge. This study aims to address this gap by investigating the optimal hydraulic calculations for designing a penstock system in Lampung, Indonesia, thereby contributing to the understanding of micro hydropower engineering. The research adopts an explanatory methodology, utilizing a case study approach in Bukit Kemuning, Lampung, Indonesia. Design data collection at the research site and relevant hydraulic design indicators from literature serve as the foundation for conducting comprehensive hydraulic calculations. The study focuses on key parameters such as penstock dimensions, power generation capacity, and energy loss assessments to inform the design process. The analysis reveals a flow rate of 11 m3/s and a flow velocity of 2.43 m/s, resulting in a targeted power output of 3234 Kilowatts. The findings underscore the critical role of key elevation parameters and design considerations in optimizing the penstock system for efficient electricity generation. Detailed calculations elucidate the determination of penstock dimensions, the evaluation of energy losses, and the consideration of surge pressure velocity to ensure system resilience. To withstand high water pressure, the research advocates for penstock pipes with specific dimensions: two pipes, each 116 m in length, with a diameter of 2.4 m and a cross-sectional area of 4.52 m2 requiring a thickness of 10 mm. The study identifies pressure reduction factors, including head loss due to penstock friction (0.169 m), friction losses (0.1635 m), and water hammer reaching 2 bar with an acceleration of 88.03 m/s2. The net head after turbulent and friction losses is determined to be 30 m. This study highlights the importance of tailored design strategies in effectively harnessing hydropower resources, offering valuable insights for micro hydropower projects globally.

O31 VALIDATION ASSESSMENT OF A CUFF-BASED CENTRAL BLOOD PRESSURE DEVICE ACCORDING TO ARTERY SOCIETY RECOMMENDATIONS

Background and objective. Uscom BP+ is a cuff-based blood pressure (BP) device designed to non-invasively estimate central BP as distinct from conventional brachial BP. This study aimed to assess the accuracy of the Uscom BP+ device compared to invasively measured BP. Methods. Automated non-invasive cuff central BP (using the Uscom BP+ device) and invasive central aortic BP (using fluid-filled catheters) were recorded simultaneously in 191 participants (65% male, aged 66±11 years) receiving coronary angiography at three independent research sites (Hobart, Australia, Perugia, Italy and Wroclaw, Poland). Validation assessment procedures were undertaken according to the Artery Society recommendations and with a minimal acceptable error of ≤5±≤8 mmHg as pass criteria. Results. Using the device default calibration technique (brachial cuff systolic [SBP] and diastolic BP [DBP]), cuff central SBP underestimated invasive central SBP [mean(SD) difference: -10.2(11.2) mmHg] and cuff central DBP overestimated invasive central DBP [mean(SD) difference: 9.7(8.6) mmHg]. When calibrating by cuff mean arterial pressure and DBP in a sub-group (n=140), SBP accuracy was improved, but variability remained high [mean(SD) difference: -6.4(13.8) mmHg, p=0.002 vs default calibration], whereas DBP accuracy and variability remained similar [mean(SD) difference: 9.0(8.4) mmHg, p=0.23 vs default calibration]. Conclusions. The Uscom BP+ cuff device does not pass the Artery Society accuracy criteria compared with invasively measured central BP.

Analysis of Quality Control for Furniture Production using Pareto Diagram and Fishbone Diagram at PT. SKM Jati

This research aims to describe Quality Control in Improving the Production Efficiency of Tables and Chairs Using Pareto and Fishbone Diagrams at PK. SKM JATI. The type of research used is qualitative descriptive with secondary and primary data, which is intended to collect data and information about a problem and facts related to the object and the research site as they are during the study. This research was conducted in Madiun Regency by interviewing the owner of PK. SKM Jati. Data collection techniques used in this study include in-depth interviews, observations, and documentation techniques to obtain accurate data for creating Pareto and fishbone diagrams. The research results show that PK. SKM Jati still faces obstacles that hinder production efficiency, with defects such as holes in the wood, moist wood, and varnish failures. The highest production failure rate is varnish failure, followed by holes in the wood, and lastly, moist wood. Through fishbone diagram analysis, four main problems are identified: people, materials, machines, and methods.

The Effect of Block Play on Fine Motor Development Among Children in Pre-School

Aims: to find out the influence of block games on the development of fine motor skills in preschool children aged 4-5 years at TKAT ARRIDLO. Methods: this study used an experimental type of research with a pre- experimental design using a pre-test and post-test design. The population in this study were all preschool children at TKAT ARRIDLO who experienced fine motor development disorders. The sampling technique is to use the entire total population so that the total sample is 17 people. The results of the study were then analyzed using the Wilcoxon test. Results: The research revealed that 11.8% of children had untested fine motor development before a block game, while 82.2% had suspect development. After the game, 29.4% had suspect development, and 70.6% had normal development. The Wilcoxon test indicated H1 as accepted. Conclusions: this shows that there is an effect of playing with blocks on the fine motor development of preschoolers at TKAT ARRIDLO. It is hoped that research sites can routinely apply games that train children's motor development, especially fine motor skills.

Geotechnical characterization of tropical soil via laboratory testing

This manuscript presents the evaluation of relevant geotechnical properties of the soil profile in the northern region of Campinas, which are important for geotechnical engineering practice and development in São Paulo State. Disturbed and undisturbed samples from the subsoil were collected through the excavation of inspection shafts at the experimental site for Soil Mechanics and Foundations research at the State University of Campinas, located at the Faculty of Civil Engineering, Architecture and Urbanism (FEC). Laboratory tests were conducted, including soil characterization, soil shear strength, soil compressibility, soil permeability, and soil suction tests. The characterization tests indicated that the upper soil layer is subject to surface effects and more direct weathering action, with noticeable variations in the liquid and plasticity limit values. The permeability test results showed no significant variation between the values obtained in the vertical and horizontal directions. The soil-water characteristic curves for the studied profile were bimodal, typical of tropical soils with macro and microaggregated structures.

The implementation of learner integration and its challenges in a selected former Model C school: Learners’ perspectives

The purpose of the study reported on here was to explore the implementation of learner integration and its challenges in a selected former Model C school. This is an expansion of the findings of a larger study, “Understanding and enhancing of learner integration in a selected ex-model C school” by Theresia Joakim Kanyopa. Based on South Africa’s history, the term “former Model C” (also ex Model C) is still commonly used to describe those schools that were formerly Whites-only but are now multiracial schools. Learner integration is a major issue in global contemporary education practice. Contemporary literature on learner integration in former Model C schools reveals complex impediments. Some of the impediments are essentially beyond the capabilities and competencies of learners, teachers and principals. In this article we argue that the lack of an effectively designed practice is central to the impediments to learner integration. This was a qualitative case study grounded within the critical research paradigm, hence the critical emancipatory research (CER) theoretical framework was adopted. We used purposive and convenience sampling to select the research site and participants. Four Grade 11 learners (2 boys and 2 girls) were purposively selected because of their availability and willingness to participate in the study. Their ages were between 16 and 19 years. Data were generated through interviews and reflective writing. Importantly, to achieve successful learner integration in former Model C schools, we affirm that these schools must revise their practices, programmes and policies to create an integrative learning environment that ensures learner safety and an environment that supports, develops end enhances their academic goals. The findings from the study indicate that learner integration in former Model C schools can be successfully done by valuing the learners’ diversities regardless of their backgrounds, ethnic groups, status or races.

Optimising Clinical Epidemiology in Disease Outbreaks: Analysis of ISARIC-WHO COVID-19 Case Report Form Utilisation.

Standardised forms for capturing clinical data promote consistency in data collection and analysis across research sites, enabling faster, higher-quality evidence generation. ISARIC and the World Health Organization have developed case report forms (CRFs) for the clinical characterisation of several infectious disease outbreaks. To improve the design and quality of future forms, we analysed the inclusion and completion rates of the 243 fields on the ISARIC-WHO COVID-19 CRF. Data from 42 diverse collaborations, covering 1886 hospitals and 950,064 patients, were analysed. A mean of 129.6 fields (53%) were included in the adapted CRFs implemented across the sites. Consistent patterns of field inclusion and completion aligned with globally recognised research priorities in outbreaks of novel infectious diseases. Outcome status was the most highly included (95.2%) and completed (89.8%) field, followed by admission demographics (79.1% and 91.6%), comorbidities (77.9% and 79.0%), signs and symptoms (68.9% and 78.4%), and vitals (70.3% and 69.1%). Mean field completion was higher in severe patients (70.2%) than in all patients (61.6%). The results reveal how clinical characterisation CRFs can be streamlined to reduce data collection time, including the modularisation of CRFs, to offer a choice of data volume collection and the separation of critical care interventions. This data-driven approach to designing CRFs enhances the efficiency of data collection to inform patient care and public health response.

Thinning relationships of woody encroachers in a US southwestern shrubland

Woody plant encroachment degrades the economic and environmental potential of drylands by altering processes such as nutrient fluxes, ecohydrology, and ecosystem services. Though past research has investigated the encroachment process, relatively little is known about post-encroachment shrub community ecology. To better quantify dynamics within post-woody encroachment shrub communities, we combined USGS lidar height data and multispectral imagery to estimate shrub density, shrub height, shrub cover, and shrub volume across the Jornada Basin Long Term Ecological Research (JRN-LTER) site in southern New Mexico, USA. Structural estimates were analyzed in search of telltale signs of community competition, specifically, the presence of thinning relationships. Results demonstrated density-dependent thinning relationships in shrub communities of creosote and mesquite, indicating a large role for competition in arid shrub communities even at relatively low shrub densities and cover (∼35% cover). In addition, shrub volume estimates better modeled the expected thinning dynamics of shrub communities than shrub canopy cover measurements. Overall, our results indicate the utility of lidar data in extending two-dimensional descriptions of woody vegetation structure (i.e., woody canopy cover) into the critical third dimension (i.e., woody plant volume), as well as the relative importance of competition and demographic bottlenecks to vegetation structure in drylands.

Diversity of Orchid species in the Tilu Mountains Region of Indonesia and the Potential for Phytochemistry

Orchids are one of the largest and globally distributed plant families. Indonesia has the most types of orchids, estimated around 20% from the total species across the world. Mainly orchids used as an ornamental plant. This research aimed to elucidate another potential of orchids as possibly for herbal medicine plant. The potential of orchids as herbal medicine has been known for a long time, but there is lack of well-documented research. The research method used in this research is exploration on predetermined research sites that were conducted in Mount Tilu, West Java on 2022. Observation data of orchids were collected on the sites and the analysis was carried out in a qualitative descriptive approach by describing the data from the research results and comparisons were made through a literature review. Based on the research results, we found about 31 species from 28 genera consisting of 24 epiphytic orchids and 7 terrestrial orchids in the Mount Tilu Kuningan area, West Java. There are 4 endemic species categorised in Java; Chilochista javanica, Crepidium koordesii, Crepidium junghuhnii, and Taeniophyllum biocellatum.

Research on Plant Landscape in Urban Wilderness Space: A Case Study of Jiangyangfan Ecological Park in Hangzhou

In recent years, with the acceleration of urbanization, the construction and management of urban wilderness space has become an important research field. The study of plantscape in urban wilderness space is one of the key points. Plant landscape is the core element of urban wilderness space, which plays an important role in improving urban environment and ecosystem service function. In this study, we chose Hangzhou Jiangyangfan Ecological Park as an example, and identified two main areas, the habitat island and the lake area, to be used as the research sites for plant species. The distribution of plant species, community structure, seasonal variation and spatial characteristics of these areas were studied and evaluated in detail. Based on the actual data collection, we will propose the following plant construction strategies: introduction of native species, creation of communities close to nature, use of temporal characteristics of plants, and the use of plants to shape the aesthetics of horticultural spaces.

Considering Race in Teacher Well-Being

Despite evidence that teachers’ professional experiences are racialized, few studies have quantitatively examined possible racial disparities in teachers’ job satisfaction. Using data from a nationally representative survey of teachers in the United States, we find that Black teachers report significantly lower job satisfaction than their White colleagues. Moreover, we find that teacher perceptions of school safety are the primary predictor of this gap in job satisfaction. We argue that centralizing race and racialized experiences in consideration of teacher job satisfaction will open new sites of inquiry in educational research and can guide educational policy to promote equity in K–12 schools.

ALLin4IPE- an international research study on interprofessional health professions education: a protocol for an ethnographic multiple-case study of practice architectures in sites of students’ interprofessional clinical placements across four universities

BackgroundThe global discourse on future health care emphasises that learning to collaborate across professions is crucial to assure patient safety and meet the changing demands of health care. The research on interprofessional education (IPE) is diverse but with gaps in curricula design and how IPE is enacted in practice.Purpose and aimsThis research project will identify. 1) how IPE in clinical placements emerges, evolves, and is enacted by students when embedded in local health care practices, 2) factors critical for the design of IPE for students at clinical placements across the four countries.MethodsA study involving four countries (Sweden, Norway, Australia and New Zealand) using the theory of practice architectures will be undertaken between 2023 and 2027. The project is designed as an international, collaborative multiple-case ethnographic study, using the theoretical framework of practice architectures (TPA). It will include four ethnographic case studies of IPE, one in each country. Data will be collected in the following sequence: (1) participant observation of students during interprofessional placements, (2) interviews with students at clinical placement and stakeholders/professionals, (3) Non-clinical documents may be used to support the analysis, and collection of photos may be use as memory aids for documenting context. An analysis of “sayings, doings and relatings” will address features of the cultural- discursive, material-economic, social-political elements making up the three key dimensions of TPA. Each of the four international cases will be analysed separately. A cross case analysis will be undertaken to establish common learning and critical IPE design elements across the four collaborating universities.DiscussionThe use of TPA framework and methodology in the analysis of data will make it possible to identify comparable dimensions across the four research sites, enabling core questions to be addressed critical for the design of IPE. The ethnographic field studies will generate detailed descriptions that take account of country-specific cultural and practice contexts. The study will also generate new knowledge as to how IPE can be collaboratively researched.

Employing popular heritage in the Iraqi theatrical actor’s performance of the play

In the history of Iraqi theater, popular heritage has always been a rich and abundant source of inspiration for artists. Heritage is considered an integral part of the cultural and social identity of the Iraqi people, as it carries within it values, traditions, and stories that constitute a large part of the collective consciousness. In this research, we will discuss how to employ popular heritage in the Iraqi theatrical actor’s performance of the play “The Thread and the Bird” as a model, as the actor aims to revive the heritage and present it in a way that is consistent with modern times, which helps to enhance cultural identity and connection to the roots, as popular heritage was used in the play The thread and the bird are touched in many ways, such as language, fashion, music, and dances. And motor performance. Through the above, the researcher will attempt to study this research in four chapters. The first chapter represents the methodological framework of the research problem around the following question: Was the Iraqi actor able to employ the popular heritage according to his motor and vocal tools in presenting the play The Thread and the Bird? Then the importance of the research and its goal through revealing and identifying the most important skills. The performance techniques that are compatible with the characters carrying the popular heritage in presenting the play The Thread and the Sparrow 0, then the limits of the research and definition of terminology. The second chapter, the theoretical framework, includes two sections: the first: the popular heritage (a historical view). The second section: the popular heritage and the performance of the theatrical actor, then what resulted from the theoretical framework. Of indicators, the third chapter includes procedures that include the research community, its sample, the research site, and sample analysis, then the fourth chapter includes results, conclusions, recommendations, and proposals, and the research concludes with sources Keywords: employment, popular heritage, actor’s performance.

Isolation, Identification and Molecular Characterization of Lumpy Skin Disease Virus for Development of Vaccine

The highly contagious Lumpy skin disease (LSD) virus has been rapidly spreading across South and East Asia, posing a grave threat to cattle populations in the region. LSD initially emerged in Bangladesh during July 2019, starting in the Chattogram district and rapidly spreading across the entire country. To investigate cases of LSD in cattle, a comprehensive study was conducted in various districts of Bangladesh, including Natore, Kurigram, Dinajpur, Dhaka, and Mymensingh. A total of 58 samples comprising blood, skin scraping, buccal swabs, and nasal swabs were collected for analysis. In order to identify LSDV, the DNA was isolated from the processed samples, and a conentional PCR method was employed utilizing the specific primer designed for the 'P-32 Gene' gene. Out of the blood samples tested, only 29.17 percent yielded a positive result for LSDV, whereas a substantial 80 percent of the skin samples tested positive. This suggests that skin scrapings are a viable specimen for LSD diagnosis using PCR. Five positive samples were partially sequenced utilizing the "ANK Gene" for molecular characterization. Bangladeshi isolates have a strong association with LSDV Kenyan (KSGP 0240, NI-2490) strains and Indian isolates (Ranchi, 2019) strains, based on multiple sequence alignment and phylogenetic analysis. Additionally, the sequencing of all the samples from the five research sites were almost identical, suggesting that a single strain is probably spreading throughout the nation. These findings emphasize the need for continuous monitoring of the genetic makeup and molecular epidemiology of LSDV, which will be useful in the future for the development of vaccines specific to the strains that are then in circulation. J. of Sci. and Tech. Res. 5(1): 31-36, 2023