Sites In France Research Articles

Rethinking partnerships between primary schools and child psychiatry public health services in times of COVID-19

On a global scale, bringing together schools and public child psychiatry services is favored to promote the development of early interventions that could improve health trajectories, from prevention to treatment, for children exposed to psychological and psychiatric difficulties. In a public health perspective, contextualizing these practices is essential in order to ensure their sustainability and efficiency. This article sheds light on the stakes of these partnerships and their advantages in responding to the health, social, and economic mark that has been left behind by thecrisis accompanying the brutal onset of the Covid-19 epidemic. Improving the health care management for children with mental health problems or problems of a psychological nature implies taking into account their environment beyond thetherapeutic framework. Interventional research, currently underway, is taking place in several sites in France: primary schools, Medical-psychological centers (CMP), and Local Committees on Mental Health (CLSM). The goal is to provide an inventory and an analysis of the partnership and interventional structures that are most efficient, based on the needs and available resources at each site. This research envisages a diversification and a contextualization of the offer of care, with great concern for equity and therapeutic efficacy, starting from school. More than simple results, our aim is to make suggestions as to how to better accompany the end of confinement and the months to come. The development of partnerships in regions that are strongly marked by social and economic inequalities is a priority in terms of public health and the direction of local policies. These partnerships would contribute to a global strategy of evaluating the needs and the personalized accompaniment of children. Formalizing the intervention with the interface being the school sector will support the school staff in overcoming the health crisis that is affecting their institution. The steady rise of CLSMs will enhance local coordination and collaboration to help the most psychologically vulnerable children and aid their parents, given their situation, to support the development of their children. Restoring human and material resources to existing structures, notably in the sector of child psychiatry so that it can accomplish its public service mission seems to be a priority today. Establishing school-CMP-CLSM partnerships can contribute to providing local policy direction in the interest of elaborating individual and collective strategies that can ensure needs-adapted care that is accessible to as many children as possible.

What do blind people "see" with retinal prostheses? Observations and qualitative reports of epiretinal implant users.

Retinal implants have now been approved and commercially available for certain clinical populations for over 5 years, with hundreds of individuals implanted, scores of them closely followed in research trials. Despite these numbers, however, few data are available that would help us answer basic questions regarding the nature and outcomes of artificial vision: what do recipients see when the device is turned on for the first time, and how does that change over time? Semi-structured interviews and observations were undertaken at two sites in France and the UK with 16 recipients who had received either the Argus II or IRIS II devices. Data were collected at various time points in the process that implant recipients went through in receiving and learning to use the device, including initial evaluation, implantation, initial activation and systems fitting, re-education and finally post-education. These data were supplemented with data from interviews conducted with vision rehabilitation specialists at the clinical sites and clinical researchers at the device manufacturers (Second Sight and Pixium Vision). Observational and interview data were transcribed, coded and analyzed using an approach guided by Interpretative Phenomenological Analysis (IPA). Implant recipients described the perceptual experience produced by their epiretinal implants as fundamentally, qualitatively different than natural vision. All used terms that invoked electrical stimuli to describe the appearance of their percepts, yet the characteristics used to describe the percepts varied significantly between recipients. Artificial vision for these recipients was a highly specific, learned skill-set that combined particular bodily techniques, associative learning and deductive reasoning in order to build a "lexicon of flashes"-a distinct perceptual vocabulary that they then used to decompose, recompose and interpret their surroundings. The percept did not transform over time; rather, the recipient became better at interpreting the signals they received, using cognitive techniques. The process of using the device never ceased to be cognitively fatiguing, and did not come without risk or cost to the recipient. In exchange, recipients received hope and purpose through participation, as well as a new kind of sensory signal that may not have afforded practical or functional use in daily life but, for some, provided a kind of "contemplative perception" that recipients tailored to individualized activities. Attending to the qualitative reports of implant recipients regarding the experience of artificial vision provides valuable information not captured by extant clinical outcome measures.

High dispersal capacity of Culicoides obsoletus (Diptera: Ceratopogonidae), vector of bluetongue and Schmallenberg viruses, revealed by landscape genetic analyses

BackgroundIn the last two decades, recurrent epizootics of bluetongue virus and Schmallenberg virus have been reported in the western Palearctic region. These viruses affect domestic cattle, sheep, goats and wild ruminants and are transmitted by native hematophagous midges of the genus Culicoides (Diptera: Ceratopogonidae). Culicoides dispersal is known to be stratified, i.e. due to a combination of dispersal processes occurring actively at short distances and passively or semi-actively at long distances, allowing individuals to jump hundreds of kilometers.MethodsHere, we aim to identify the environmental factors that promote or limit gene flow of Culicoides obsoletus, an abundant and widespread vector species in Europe, using an innovative framework integrating spatial, population genetics and statistical approaches. A total of 348 individuals were sampled in 46 sites in France and were genotyped using 13 newly designed microsatellite markers.ResultsWe found low genetic differentiation and a weak population structure for C. obsoletus across the country. Using three complementary inter-individual genetic distances, we did not detect any significant isolation by distance, but did detect significant anisotropic isolation by distance on a north-south axis. We employed a multiple regression on distance matrices approach to investigate the correlation between genetic and environmental distances. Among all the environmental factors that were tested, only cattle density seems to have an impact on C. obsoletus gene flow.ConclusionsThe high dispersal capacity of C. obsoletus over land found in the present study calls for a re-evaluation of the impact of Culicoides on virus dispersal, and highlights the urgent need to better integrate molecular, spatial and statistical information to guide vector-borne disease control.Graphical

Implementation of sequential cropping into JULESvn5.2 land-surface model

Abstract. Land-surface models (LSMs) typically simulate a single crop per year in a field or location. However, actual cropping systems are characterized by a succession of distinct crop cycles that are sometimes interspersed with long periods of bare soil. Sequential cropping (also known as multiple or double cropping) is particularly common in tropical regions, where the crop seasons are largely dictated by the main wet season. In this paper, we implement sequential cropping in a branch of the Joint UK Land Environment Simulator (JULES) and demonstrate its use at sites in France and India. We simulate all the crops grown within a year in a field or location in a seamless way to understand how sequential cropping influences the surface fluxes of a land-surface model. We evaluate JULES with sequential cropping in Avignon, France, providing over 15 years of continuous flux observations (a point simulation). We apply JULES with sequential cropping to simulate the rice–wheat rotation in a regional 25 km resolution gridded simulation for the northern Indian states of Uttar Pradesh and Bihar and four single-grid-box simulations across these states, where each simulation is a 25 km grid box. The inclusion of a secondary crop in JULES using the sequential cropping method presented does not change the crop growth or development of the primary crop. During the secondary crop growing period, the carbon and energy fluxes for Avignon and India are modified; they are largely unchanged for the primary crop growing period. For India, the inclusion of a secondary crop using this sequential cropping method affects the available soil moisture in the top 1.0 m throughout the year, with larger fluctuations in sequential crops compared with single-crop simulations even outside the secondary crop growing period. JULES simulates sequential cropping in Avignon, the four India locations and the regional run, representing both crops within one growing season in each of the crop rotations presented. This development is a step forward in the ability of JULES to simulate crops in tropical regions where this cropping system is already prevalent. It also provides the opportunity to assess the potential for other regions to implement sequential cropping as an adaptation to climate change.

A Lower Cretaceous Lagerstätte from France: a taphonomic overview of the Angeac‐Charente vertebrate assemblage

Terrestrial ecosystems from the Lower Cretaceous of Europe and bonebeds formed in swampy environments are poorly known. The Berriasian-early Valanginian Angeac-Charente site in France represents an example of both. Nine field campaigns have yielded thousands of fossils of over a hundred taxa, including 16 taxa from vertebrate macroremains with numerous trample and crocodile bite marks; 22 taxa from the abundant vertebrate microremains; >10 vertebrate coprolite morphotypes with plant and vertebrate inclusions; abundant sauropod and stegosaur tracks including some preserved in ‘4-D’; termite coprolites; mollusc moulds; ostracods and plants, including coniferous wood, cones, leaves and cuticle fragments, charophytes and pollen. The richness, diversity and preservation of the fossils qualify the site as a fossil-Lagerstätte. The site represents a ‘snapshot’ into a Lower Cretaceous ecosystem. This is supported by REE analyses of biogenic apatite and sediment samples, the fossils being found in a single stratigraphical interval and the record of sedimentological and taphonomic ‘frozen scenes’. The Angeac-Charente bonebed is highly diverse, dominated by an ornithomimosaur taxon, and contains both macro- and microfossils. This indicates a complex formation, likely primarily influenced by ecological and biologic processes, but also significant physical processes. These include crocodyliform predation and/or scavenging on turtles, ornithomimosaurs and fishes; probable mass mortality occurrence of an ornithomimosaur herd; possible social behaviour of stegosaurs; limited hydraulic transport of most sauropod bones and intense dinoturbation.

Novasep equipment unit goes to Sartorius

Novasep, a manufacturer of active pharmaceutical ingredients (APIs), will sell its chromatography equipment division to Sartorius. The deal involves operations at Novasep sites in France, the US, C...

Use of voluntary environmental contracts for wetland governance in the European Mediterranean region

Ramsar sites are internationally protected wetlands designated to maintain an international network for biodiversity conservation and sustain human life. There are often conflicting interests for site management that lead to decisions based on sectoral policies or local approaches that have poor outcomes in terms of effectiveness and trade-offs. The European Water Framework Directive (2000/60/EC) requires European countries to foster an integrated approach for wetland management through collaborative governance processes. The environmental contract is a tool that has been developed and implemented to set the ground for voluntary-based commitments for the sustainable governance of water systems. This paper reports on the results of the first study investigating the implementation of voluntary environmental contracts at the Mediterranean European level. Our results indicate that Ramsar sites in the region have made great progress with the development of management plans, yet many sites still lack governance schemes involving stakeholders. Voluntary environmental contracts have been widely used in Ramsar sites in France and Italy, but only a few other European countries have started implementing them. We have identified the need to further adapt the tool to local and national contexts, as well as the need for legislation, to promote upscaling at the regional scale.

Adaptation of recombinant activated factor VII in the treatment of acquired haemophilia A: Results from a prospective study (ACQUI-7) in France

BackgroundThe efficacy and safety of recombinant activated factor VII (rFVIIa; NovoSeven®) in patients with acquired haemophilia A (AHA) are established; however, data on daily use in clinical practice for bleeding episodes are limited. The ACQUI-7 study aimed to provide additional data on managing bleeding episodes with rFVIIa in patients with AHA. MethodsACQUI-7, a prospective, observational, multicentre study in 20 sites in France, recruited patients from 2010 to 2013. Treatment was by physician’s judgement and therapeutic practice at each site. Inclusion criteria: anti-factor VIII (FVIII) auto-antibodies ​> ​1 Bethesda Unit, FVIII activity ​< ​50% and bleeding episodes treated with rFVIIa. Data collected included description of patient characteristics, bleeding episodes leading to haemostatic treatment and rFVIIa therapy. ResultsA total of 27 patients with 27 bleeding episodes (24 [88.9%] severe) were treated with first-line rFVIIa. Most (81.5%) were affected in more than 1 site (44.4% in muscle). Nineteen patients (70.4%) were treated for up to 5 days; 24 (88.9%) had their bleeding episode controlled (including 21 severe bleeds). The maximum daily dose for controlling severe bleeds decreased from 900 ​μg/kg/24 ​h on day 1–630 ​μg/kg/24 ​h on day 5, with a corresponding reduction in maximum number of daily injections from 10 to 7. The use of rFVIIa in this population did not raise any safety concerns. ConclusionsThese real-world data confirm the effectiveness and safety of rFVIIa and provide additional information on the daily use of rFVIIa to support the management of patients with AHA.

Multi‐species occupancy models: an effective and flexible framework for studies of insect communities

Entomological studies often aim to estimate species distribution, community composition, or species‐richness patterns. False absences can, however, bias these estimates and should consequently not be overlooked in insect studies. Multi‐species occupancy models (MSOMs) afford a flexible solution to cover the main topics in ecological entomology while dealing with detectability issues. We sampled Orthoptera communities at 81 mountain grasslands sites in France, using three sampling techniques: sighting, listening, and sweep netting. Five plots were sampled per site. This sampling design allowed MSOMs to be used to estimate richness, occupancy, and detection probabilities while accounting for the effect of covariates. We also used MSOMs to evaluate the efficiency of the survey design and to assess the effects of sampling optimisation. The estimates obtained for altitudinal distribution were reliable, with known species distributions confirming the relevance of MSOMs to model the effects of covariates on Orthoptera communities. The species‐specific detection probability was often less than one and varied with the detection technique used and the grass height, confirming the need to deal with detection issues in orthopteran studies. We estimated an inventory completeness superior to 0.80 for 93% of the sites, and an overall detection probability superior to 0.95 for 52% of the species, suggesting the sampling design was suitable for studying occupancy in Orthoptera communities. We also found that the sweep netting step may be omitted or the number of plots reduced without affecting species detectability or inventory completeness. Those recommendations may help to optimise future sampling strategies.

Abundance and seasonal growth of epiphytic ferns at three sites along a rainfall gradient in Western Europe

Vascular epiphytes in temperate zones are allegedly rare due to climatic constraints, such as drought and freezing temperatures during winter. Here, we investigate how abundance and seasonal growth of epiphytic polypod ferns in Western Europe are influenced by annual rainfall and temperature. We recorded abundances and growth of epiphytic polypod ferns over two years at three sites along a rainfall gradient from Southwest Ireland to Northwest Germany, spanning a two-fold difference in annual rainfall. We also investigated plant maturation and recorded key climate characteristics (rainfall and temperature). Abundance of epiphytic polypod ferns strongly decreased from the wetter end of the rainfall gradient in Ireland towards the drier end in Germany. Yet, frond turnover was equally high at all study sites during summer months, and equally low during winter. Plants showed only little to no expansive growth during the study period, except for the study site in France in the middle of the gradient, where the total number of fronds strongly increased. Similarly, we found an increase in the proportion of spore-bearing fronds at the French study site, whereas there was hardly any change between years at the other two sites. The observed growth patterns are inconsistent with the current differences in abundance of epiphytic ferns. We suggest that these differences are due to poor germination of spores under drier conditions and that small differences in annual growth have contributed to the current abundances over an extended time period. Surprisingly, seasonal frond productivity appeared to be unaffected by the amount of annual rainfall and average temperatures, although it seems likely that these factors do play a role during gametophyte establishment.

Atmospheric iodine, selenium and caesium depositions in France: I. Spatial and seasonal variations

The spatial distribution and seasonal variations of atmospheric iodine (I), selenium (Se) and caesium (Cs) depositions remain unclear and this precludes adequate inputs for biogeochemical models. We quantified total concentrations and fluxes of these elements in rainfalls from 27 monitoring sites in France with contrasted climatic conditions; monthly measurements were taken over one year (starting in 2016/09). Since speciation of I and Se can impact their behaviour in the environment, analysis of their inorganic compounds was also conducted. Our results showed that annual I concentrations in rainfall were much higher than those of Se and Cs (annual means = 1.56, 0.044 and 0.005 μg L−1, respectively). The annual iodine concentrations were highly positively correlated with those of marine elements (i.e. Na, Cl and Mg), involving higher I concentrations under oceanic climate than for transition, continental and mountainous ones. Furthermore, common patterns were found between Se concentrations and both marine and terrestrial components consistent with the various sources of Se in atmosphere. The association of Cs with two anthropogenic components (i.e. NH4+ and NO3−) used in agriculture supports the hypothesis of its terrestrial origin (i.e. from atmospheric dusts) in rainfall. We found higher rainfall concentrations of I during the warmest months for all climates. However, no specific seasonal trend occurred for Se and Cs. On annual average, rainfall contained mostly unidentified selenium compounds (inorganic Se proportions = 25–54%) and equal proportions of inorganic and unidentified I compounds. Concentrations of iodate were higher under oceanic climate consistent with an iodine marine-origin.

A core seed endophytic bacterial community in the hyperaccumulator Noccaea caerulescens across 14 sites in France

Seed endophytic bacterial (SEB) communities have been little studied to date, although these microorganisms may assist seeds and seedlings to cope with environmental stresses. Hyperaccumulators have become models for studying plant adaptation to extreme environments, such as metalliferous soils. This work aims to characterize the endophytic bacterial communities associated with hyperaccumulating seeds collected from their native ecosystems (nonmetalliferous or calamine soils). Using 16S rRNA metabarcoding, endophytic bacterial communities were characterized for seeds from 14 Noccaea caerulescens populations. Plant genomes and environmental properties of the sites had previously been described. In all plant populations the metabarcoding revealed a large SEB core microbiome composed of the main phyla and sub-phyla: ɣ-Proteobacteria (56.56%) > α-Proteobacteria (32.23%) > Actinobacteria (7.93%) > Firmicutes (3.78%). According to the literature, some operational taxonomic units (OTUs) found in the core microbiome may be related to “plant growth promoting endophytes” (PGPE). The changes in the relative abundance of phyla/subphyla were correlated to altitude, pH, and soil exchangeable elements (Al, Mg, Cd, Mn, Zn), yet the main parameters observed to drive SEB diversity were the plants’ genetics. The distinction between populations based on calamine soils and nonmetalliferous soils failed to explain the SEB communities’ structural dissimilarities. In fact, the factor that best explained these dissimilarities was the plant’s genetic subunit. Future research, based on transcriptomic approaches, should study the ecological roles of the SEB communities and their involvement in the adaptation of hyperaccumulators to metalliferous soils.

Desperately seeking stress: A pilot study of cortisol in archaeological tooth structures.

Cortisol is a glucocorticoid hormone produced through activation of the hypothalamic pituitary adrenal axis. It is known as the "stress hormone" for its primary role in the body's stress response and has been the focus of much modern clinical research. Within archaeology, only a few studies have analyzed cortisol in human remains and these have been restricted to hair (Webb et al., 2010; Webb, White, van Uum, & Longstaffe, 2015a; Webb, White, van Uum, & Longstaffe, 2015b). This study examines the utility of dentine and enamel, which survive well archaeologically, as possible reservoirs for detectable levels of cortisol. Then, 69 teeth from 65 individuals from five Roman and Post-Roman sites in France were tested via competitive enzyme-linked immunosorbent assay (ELISA) to assess and quantify the cortisol concentrations present within tooth dentine and enamel. In both tooth dentine and enamel, detectable concentrations of cortisol were identified in multiple teeth. However, concentrations were low and not all teeth yielded results that were measurable through cortisol ELISA. Differences in cortisol values between dentine and enamel could suggest different uptake mechanisms or timing. These results suggest that cortisol is incorporated within tooth structures and merits further investigation in both modern and archaeological contexts. Analysis of the results through liquid chromatographic-mass spectrometry would verify current results and might yield values that could be better integrated with published cortisol studies. Future studies of cortisol in tooth structures would greatly expand the research potential of cortisol in the past and could have implications for studies of human stress across deep time.

The incidence of psychotic disorders among migrants and minority ethnic groups in Europe: findings from the multinational EU-GEI study.

In Europe, the incidence of psychotic disorder is high in certain migrant and minority ethnic groups (hence: 'minorities'). However, it is unknown how the incidence pattern for these groups varies within this continent. Our objective was to compare, across sites in France, Italy, Spain, the UK and the Netherlands, the incidence rates for minorities and the incidence rate ratios (IRRs, minorities v. the local reference population). The European Network of National Schizophrenia Networks Studying Gene-Environment Interactions (EU-GEI) study was conducted between 2010 and 2015. We analyzed data on incident cases of non-organic psychosis (International Classification of Diseases, 10th edition, codes F20-F33) from 13 sites. The standardized incidence rates for minorities, combined into one category, varied from 12.2 in Valencia to 82.5 per 100 000 in Paris. These rates were generally high at sites with high rates for the reference population, and low at sites with low rates for the reference population. IRRs for minorities (combined into one category) varied from 0.70 (95% CI 0.32-1.53) in Valencia to 2.47 (95% CI 1.66-3.69) in Paris (test for interaction: p = 0.031). At most sites, IRRs were higher for persons from non-Western countries than for those from Western countries, with the highest IRRs for individuals from sub-Saharan Africa (adjusted IRR = 3.23, 95% CI 2.66-3.93). Incidence rates vary by region of origin, region of destination and their combination. This suggests that they are strongly influenced by the social context.

Becoming a transcultural psychotherapist: Qualitative study of the experience of professionals in training in a transcultural psychotherapy group

Transcultural psychotherapy is an original therapeutic technique designed to respond to difficulties encountered in psychiatric treatment for migrants. Today, this psychotherapy is formalized and it is in use at numerous sites in France and internationally. An increasing number of professionals are seeking training in this method. We sought to explore the experiences of these trainees, at their entry in the group and during their training. This qualitative study used focus groups to interview trainees participating in a transcultural psychotherapy training group. The thematic analysis generated two domains of experience: the emotional and personal experience within the transcultural group, including the private feelings of the trainee-participants, their initial difficulties, and the changes in these feelings; and their perception of this specific type of care, that is, their perspectives on transcultural psychotherapy and its most original aspects. Based on the narratives of trainees in this program, we conclude that becoming a transcultural psychotherapist involves a process not only of cultural decentering but also of professional decentering. This decentering cannot be learned theoretically: it must be experienced, for a long enough time to become imbued with it and to allow oneself to modify one's practices. After sufficient time in the group, the trainees succeed in extricating themselves, little by little, from their ethnocentric vision of psychotherapy, and come to tolerate and then integrate new ways of doing and thinking.

Micromegas chambers for the ATLAS New Small Wheel upgrade

The ATLAS collaboration at LHC has chosen the resistive Micromegas technology, along with the small-strip Thin Gap Chambers (sTGC), for the high luminosity upgrade of the first muon station in the high-rapidity region, the so called New Small Wheel (NSW) project. After the R&D, design and prototyping phase, the series production Micromegas quadruplets are being constructed at the involved construction sites in France, Germany, Italy, Russia and Greece. At CERN, the final validation and the integration of the modules in Sectors are in progress. The installation of the first NSW, the NSW-A is foreseen for the LHC long shutdown in 2020. The construction of the four types of large size quadruplets, all having trapezoidal shapes with surface areas between 2 and 3 m2, will be reviewed. The achievement of the requirements for these detectors revealed to be even more challenging than expected, when scaling from the small prototypes to the large dimensions. The encountered problems will be described, to a large extent common to other micro-pattern gaseous detectors, along with the adopted solutions. Final quality assessment and validation results on the achieved mechanical precision, on the High-Voltage stability during operation with and without irradiation will be reviewed together with the most relevant steps and results of the modules integration into sectors.

SYNERGISTIC USE OF SENTINEL-1 AND SENTINEL-2 TIME SERIES FOR POPLAR PLANTATIONS MONITORING AT LARGE SCALE

Abstract. The current context of availability of Earth Observation satellite data at high spatial and temporal resolutions makes it possible to map large areas. Although supervised classification is the most widely adopted approach, its performance is highly dependent on the availability and the quality of training data. However, gathering samples from field surveys or through photo interpretation is often expensive and time-consuming especially when the area to be classified is large. In this paper we propose the use of an active learning-based technique to address this issue by reducing the labelling effort required for supervised classification while increasing the generalisation capabilities of the classifier across space. Experiments were conducted to identify poplar plantations in three different sites in France using Sentinel-2 time series. In order to characterise the age of the identified poplar stands, temporal means of Sentinel-1 backscatter coefficients were computed. The results are promising and show the good capacities of the active learning-based approach to achieve similar performance (Poplar F-score &amp;amp;geq; 90%) to traditional passive learning (i.e. with random selection of samples) with up to 50% fewer training samples. Sentinel-1 annual means have demonstrated their potential to differentiate two stand ages with an overall accuracy of 83% regardless of the cultivar considered.

A new multiplex real-time PCR assay to improve the diagnosis of shellfish regulated parasites of the genus Marteilia and Bonamia

Aquaculture including shellfish production is an important food resource worldwide which is particularly vulnerable to infectious diseases. Marteilia refringens, Bonamia ostreae and Bonamia exitiosa are regulated protozoan parasites infecting flat oysters Ostrea edulis that are endemic in Europe. Although some PCR assays have been already developed for their detection, a formal validation to assess the performances of those tools is often lacking. In order to facilitate the diagnosis of flat oyster regulated diseases, we have developed and evaluated a new multiplex Taqman® PCR allowing the detection of both M. refringens and Bonamia sp. parasites in one step.First part of this work consisted in assessing analytical sensitivity and specificity of the new PCR assay. Then, diagnostic performances were assessed by testing a panel of field samples with the new real-time PCR and currently recommended conventional PCR methods for the detection of M. refringens and Bonamia sp. Samples were collected from the main flat oyster production sites in France (N = 386 for M. refringens and N = 349 for B. ostreae). In the absence of gold standard, diagnostic sensitivity and specificity of the new PCR were estimated through Bayesian latent class analysis (DSe 87,2% and DSp 98,4% for the detection M. refringens, DSe 77,5% and DSp 98,4% for the detection of Bonamia sp.). Those results suggest equivalent performances for the detection of Bonamia sp. and an improved sensitivity for the detection of M. refringens compared to commonly used conventional protocols. Finally, the new PCR was evaluated in the context of an inter-laboratory comparison study including 17 European laboratories. Results revealed a very good reproducibility with a global accordance (intra-laboratory precision) >96% and a global concordance (inter-laboratory precision) >93% for both targets, demonstrating that this new tool is easily transferable to different laboratory settings.This is the first assay designed to detect both Marteilia refringens and Bonamia sp. in a single step and it should allow reducing the number of analysis to monitor both diseases, and where relevant to demonstrate freedom from infection.

Avadomide plus obinutuzumab in patients with relapsed or refractory B-cell non-Hodgkin lymphoma (CC-122-NHL-001): a multicentre, dose escalation and expansion phase 1 study

Avadomide (CC-122) is a novel oral cereblon-modulating agent with promising activity in non-Hodgkin lymphoma. We aimed to examine the safety and preliminary activity of avadomide plus obinutuzumab in patients with relapsed or refractory non-Hodgkin lymphoma. CC-122-NHL-001 was a phase 1b dose escalation and expansion study at eight sites in France, Italy, and the Netherlands. Eligible patients (aged ≥18 years) had histologically confirmed CD20-positive relapsed or refractory non-Hodgkin lymphoma, had an Eastern Cooperative Oncology Group performance status of 0 or 1, and had received previous treatment. In the dose expansion phase, only patients with previously treated relapsed or refractory follicular lymphoma (grade 1, 2, or 3a) were included. Avadomide was administered in escalating doses and two formulations: active pharmaceutical ingredient in capsule in 1·0 mg, 2·0 mg, 3·0 mg, and 4·0 mg doses and as formulated capsules in 3·0 mg and 4·0 mg doses orally once daily on days 1-5 followed by 2 days off (5-7-day schedule) every week of each 28-day cycle. Obinutuzumab 1000 mg was administered intravenously on days 2, 8, and 15 of cycle 1 and day 1 of cycles 2-8. Primary objectives were to determine the safety and tolerability, the non-tolerated dose, maximum tolerated dose, and recommended phase 2 dose (RP2D). All patients who received treatment were included in the safety analyses. Efficacy-evaluable patients completed at least one cycle of treatment and had baseline and at least one post-baseline assessment. The study is registered with ClinicalTrials.gov, NCT02417285 and EudraCT 2014-003333-26, and is ongoing. Between June 24, 2015, and Dec 5, 2018, 73 patients were enrolled and treated; 19 had diffuse large B-cell lymphoma, 53 follicular lymphoma, and one marginal zone lymphoma. Median follow-up was 253 days (IQR 127-448). The median number of previous anticancer regimens was three (IQR 2-4). The maximum tolerated dose and non-tolerated dose were not reached in the dose escalation phase. On the basis of safety and pharmacokinetic-pharmacodynamic data, the avadomide RP2D was established as 3·0 mg as formulated capsules on a 5-7-day schedule in combination with 1000 mg of obinutuzumab. Patients enrolled in the expansion cohort received the established RP2D of avadomide. Across all doses, three patients had dose-limiting toxicities; one patient treated at the RP2D had dose-limiting toxicity (grade 3 sepsis). The most common adverse events of grade 3 and above were neutropenia (41 [56%] of 73) and thrombocytopenia (17 [23%] of 73). 34 (47%) patients had serious adverse events, which were considered to be avadomide-related in 23 (32%) of 73 patients and obinutuzumab-related in 20 (27%) of 73 patients. Two treatment-related deaths occurred, one owing to tumour flare and one from acute myeloid leukaemia after study discontinuation. Avadomide plus obinutuzumab has a manageable toxicity, being a tolerable treatment option for most patients. Although the prespecified threshold for activity was not met in the trial, we believe that the preliminary antitumour activity of cereblon modulators plus next-generation anti-CD20 antibodies in heavily pretreated relapsed or refractory non-Hodgkin lymphoma warrants further investigation as a chemotherapy-free option in this setting. Celgene Corporation.

Evaluation of Nearshore and Offshore Water Quality Assessment Using UAV Multispectral Imagery

A cost-effective technology has emerged which combines multispectral sensors mounted on Unmanned Aerial Vehicles (UAVs). This technology has a promising potential for monitoring water quality in coastal environments. Our study aimed at evaluating this technology to infer the spatial distribution of chlorophyll a concentration [Chl-a] (in µg·L−1) and turbidity (FNU) in surface waters. The multispectral sensor measured reflectance at 4 distinct wavelength bands centered on 448 nm, 494 nm, 550 nm and 675 nm, hence providing 4 datasets {R(448), R(494), R(550), R(675)}. We investigated the potential of estimating [Chl-a] and turbidity based on reflectance ratios and indexes calculated from two different wavelength bands. The calibration functions were formulated based on the property that any of the reflectance measurements was linearly correlated to any other one. The calibration was performed from 35 measurements of reflectance, [Chl-a] and turbidity collected in seven sites in the U.K. between May and August 2017. Two calibration functions derived from the index δ=(R(550) − R(448))/(R(550) + R(448)) presented the best fit and explained 78% of the total variance for [Chl-a] and 74% for turbidity measurements, respectively. Calibration functions were then inversed to estimate [Chl-a] and turbidity from reflectance measurements. Finally, we performed a validation test using independent measurements from three sites in France, in July 2017. The resulting maps show a pattern with higher [Chl-a] in lower turbidity areas. However, discrepancies between the observed and re-calculated values and difficulties in validating low turbidity values suggest that site-specific calibrations should be performed at each investigated location.