Position Of Site Research Articles

Karyotype Description and Comparative Chromosomal Mapping of 5S rDNA in 42 Species.

This study was conducted to evaluate the 5S rDNA site number, position, and origin of signal pattern diversity in 42 plant species using fluorescence in situ hybridization. The species were selected based on the discovery of karyotype rearrangement, or because 5S rDNA had not yet been explored the species. The chromosome number varied from 14 to 160, and the chromosome length ranged from 0.63 to 6.88 μm, with 21 species having small chromosomes (<3 μm). The chromosome numbers of three species and the 5S rDNA loci of nineteen species are reported for the first time. Six 5S rDNA signal pattern types were identified. The 5S rDNA varied and was abundant in signal site numbers (2-18), positions (distal, proximal, outside of chromosome arms), and even in signal intensity. Variation in the numbers and locations of 5S rDNA was observed in 20 species, whereas an extensive stable number and location of 5S rDNA was found in 22 species. The potential origin of the signal pattern diversity was proposed and discussed. These data characterized the variability of 5S rDNA within the karyotypes of the 42 species that exhibited chromosomal rearrangements and provided anchor points for genetic physical maps.

Southbound transmission of metallurgy: new excavations at Jicha in the Hengduan Mountains, Yunnan

AbstractJicha is a Bronze Age settlement located next to the upper Mekong River in the Hengduan Mountains of Yunnan, south-west China. Recent excavations have revealed details of successive occupation and copper-base industrial activity. The site's position and chronology provide evidence of north–south demographic movement and technological transmission along the eastern Qinghai-Tibet Plateau corridor.

Reactivity of amino acids and short peptide sequences: identifying bioactive compounds via DFT calculations.

Bioactive peptides are short amino acid sequences that play important roles in various physiological processes, including antioxidant and protective effects. These compounds can be obtained through protein hydrolysis and have a wide range of potential applications in a variety of areas. However, despite the potential of these compounds, more in-depth knowledge is still necessary to better understand details regarding their chemical reactivity and electronic properties. In this study, we used molecular modeling techniques to investigate the electronic structure of isolated amino acids (AA) and short peptide sequences. Details on the relative alignments between the frontier electronic levels, local chemical reactivity and donor-acceptor properties of the 20 primary amino acids and some di- and tripeptides were evaluated in the framework of the density functional theory (DFT). Our results suggest that the electronic properties of isolated amino acids can be used to interpret the reactivity of short sequences. We found that aromatic and charged amino acids, as well as Methionine, play a key role in determining the local reactivity of peptides, in agreement with experimental data. Our analyses also allowed us to identify the influence of the relative position of AA and terminations on the local reactivity of the sequences, which can guide experimental studies and help to propose/evaluate possible mechanisms of action. In summary, our data indicate that the position of active sites of polypeptides can be predicted from short sequences, providing a promising strategy for the synthesis and bioprospection of new optimized compounds.

Language models can identify enzymatic binding sites in protein sequences

Recent advances in language modeling have had a tremendous impact on how we handle sequential data in science. Language architectures have emerged as a hotbed of innovation and creativity in natural language processing over the last decade, and have since gained prominence in modeling proteins and chemical processes, elucidating structural relationships from textual/sequential data. Surprisingly, some of these relationships refer to three-dimensional structural features, raising important questions on the dimensionality of the information encoded within sequential data. Here, we demonstrate that the unsupervised use of a language model architecture to a language representation of bio-catalyzed chemical reactions can capture the signal at the base of the substrate-binding site atomic interactions. This allows us to identify the three-dimensional binding site position in unknown protein sequences. The language representation comprises a reaction-simplified molecular-input line-entry system (SMILES) for substrate and products, and amino acid sequence information for the enzyme. This approach can recover, with no supervision, 52.13% of the binding site when considering co-crystallized substrate-enzyme structures as ground truth, vastly outperforming other attention-based models.

Stable isotope insights into arthropod food chains and nitrogen cycling in a rehabilitated tailings chronosequence

Field studies in post-mining sites on epigeic invertebrate groups are scarce despite their importance in pedogenesis and ecosystem multifunctionality. This research investigated the diversity, abundance and succession of aboveground invertebrates in a rehabilitated Pb/Zn tailings chronosequence of 5, 20 and 35 years. The study also explored the trophic relationship of selected arthropods and characterized the nitrogen (N) cycle using stable isotope measurements. The abundance and species richness in most investigated groups and the dominance index Berger–Parker (BP) of aboveground invertebrates increased with rehabilitation age from 0.17 BP in early to 0.31 BP in late stage. Elemental and stable isotope ratio analysis showed that N and C soil content increased and the C/N ratio decreased with age, yet despite this increased N availability in the system, the maturing N-cycle used N efficiently. The lack of large N losses from the system despite substantial atmospheric deposition inputs was indicated by the fact that N isotope ratios (δ15N) in plants and animals became significantly more negative with rehabilitation age, −6.0 δ15N for plants, −5.0 δ15N for herbivores and 3.0 δ15N for carnivores. The length of the invertebrate food chain expanded by more than half a trophic level (2.7‰ δ15N) for top predator Coleoptera from early to late stage, probably reflecting more complex food webs including intra-guild predation in older communities. In conclusion, δ15N measurements in plants and animals provided novel insights into the N-cycle, accumulative N flows and the trophic position in post-mining sites. It is proposed that isotope ratio measurements could be used as easy-to-measure, integrating indicators of nutrient cycling and the soil food web complexity of rehabilitated mine tailings and similar soil ecosystems.

Optimal placement of distributed generation in power distribution system and evaluating the losses and voltage using machine learning algorithms

As the modern power system continues to grow in size, complexity, and uncertainty, traditional methods may occasionally prove insufficient in addressing the associated challenges. The improper location of distributed generation varies the voltage profile, increases losses and compromises network capacity. Machine learning algorithms predict accurate site positions, and network reconfiguration improves the capacity of the power system. The proposed algorithm is a hybrid of machine learning and deep learning algorithms. It cascades Support Vector Machine as the main model and uses Random Forest and Radial Neural Networks as classification algorithms for accurately predicting DG position. The non-linearity characteristics of the DG problem are directly mapped to the proposed algorithms. The proposed algorithm is employed on familiar test setups like the IEEE 33-bus and 69-bus distribution systems using MATLAB R2017 as simulation software. The R-squared (R2) values for all parameters yield a value of 1, while the MAPE values are minimal for the proposed cascaded algorithm in contrast to other algorithms of LSTM, CNN, RNN and DQL.

Multi-scale palaeolandscape reconstruction at the Upper Paleolithic Byki sites, central East European Plain

The Byki complex on the left bank of the Seim River (Desna basin) comprises eight sites, including multilayer ones, differing chronologically and culturally. The dates of key cultural layers are from 21 to 18 thousand BP. Questioning the use of local landscapes by prehistoric people and the reasons for repeated occupation of the area required a paleolandscape reconstruction. For this purpose, a set of methods were applied on a research scale from local (archaeological excavation) to regional (within a radius of several kilometres). The site's position in the regional topography has been studied using GIS methods, for the nearest encirclement of Byki a detailed digital terrain model was constructed. The near-surface stratigraphy was studied by ground penetrating radar (GPR) and magnetic survey, coring and archaeological excavations. This resulted in the creation of a map of natural depressions layout and their three-dimensional visualisation. It was found that by the time people first arrived, the natural relief was a combination of sand dunes and rounded thermokarst sinkholes. The sediments filling the sinkholes indicate the over-watered conditions made them unsuitable for human habitation (the cultural layers do not extend into the sinkholes), but they could provide a source of water. Most of the sites are found on top of an aeolian barkhan. The distance maps showed that the sites’ location provided the best visibility in all directions. Thus, this atypical location of Byki sites at significant distances from the nearest rivers can be explained by a combination of several advantages: location on the edge of a valley in terms of distance vision, local topographic diversity that provided comfortable living conditions and possibly a water supply.

Efficient Sodium Transmembrane Permeation through Helically Folded Nanopores with Natural Channel-Like Ion Selectivity.

The selective transmembrane permeation of sodium ions achieved by biomimetic chemistry shows great potential to solve the problem of sodium ion transport blockade in diseases, but its implementation faces enormous difficulties. Herein, we design and synthesize a series of helically folded nanopores by employing a quinoline-oxadiazole structural sequence to finely replicate the pentahydrate structure of sodium ions. Surprisingly, these nanopores are capable of achieving sodium transmembrane permeation with ion selectivity at the level of natural sodium channels, as observed in rationally designed nanopores (M1-M5) with Na+/K+ ion selectivity ratio of up to 20.4. Moreover, slight structural variations in nanopore structures can switch ion transport modes between the channel and carrier. We found that, compared to the carrier mode, the channel mode not only transports ions faster but also has higher ion selectivity during transmembrane conduction, clearly illustrating that the trade-off phenomenon between ion selectivity and transport activity does not occur between the two transport modes of channel and carrier. At the same time, we also found that the spatial position and numbers of coordination sites are crucial for the sodium ion selectivity of the nanopores. Moreover, carrier M1 reported in this work is totally superior to the commercial Na+ carrier ETH2120, especially in terms of Na+/K+ ion selectivity, thus being a potentially practical Na+ carrier. Our study provides a new paradigm on the rational design of sodium-specific synthetic nanopores, which will open up the possibility for the application of artificial sodium-specific transmembrane permeation in biomedicine and disease treatment.

Network of epistatic interactions in an enzyme active site revealed by large-scale deep mutational scanning

Cooperative interactions between amino acids are critical for protein function. A genetic reflection of cooperativity is epistasis, which is when a change in the amino acid at one position changes the sequence requirements at another position. To assess epistasis within an enzyme active site, we utilized CTX-M β-lactamase as a model system. CTX-M hydrolyzes β-lactam antibiotics to provide antibiotic resistance, allowing a simple functional selection for rapid sorting of modified enzymes. We created all pairwise mutations across 17 active site positions in the β-lactamase enzyme and quantitated the function of variants against two β-lactam antibiotics using next-generation sequencing. Context-dependent sequence requirements were determined by comparing the antibiotic resistance function of double mutations across the CTX-M active site to their predicted function based on the constituent single mutations, revealing both positive epistasis (synergistic interactions) and negative epistasis (antagonistic interactions) between amino acid substitutions. The resulting trends demonstrate that positive epistasis is present throughout the active site, that epistasis between residues is mediated through substrate interactions, and that residues more tolerant to substitutions serve as generic compensators which are responsible for many cases of positive epistasis. Additionally, we show that a key catalytic residue (Glu166) is amenable to compensatory mutations, and we characterize one such double mutant (E166Y/N170G) that acts by an altered catalytic mechanism. These findings shed light on the unique biochemical factors that drive epistasis within an enzyme active site and will inform enzyme engineering efforts by bridging the gap between amino acid sequence and catalytic function.

Microplastics in the Water Column of the Rhine River Near Basel: 22 Months of Sampling.

Measured microplastic concentrations in river surface waters fluctuate greatly. This variability is affected by season and is codriven by factors, such as sampling methodologies, sampling site, or sampling position within site. Unfortunately, most studies comprise single-instance measurements, whereas extended sampling periods are better suited to assessing the relevance of such factors. Moreover, microplastic concentrations in riverine water column remain underexplored. Similar to the oceans, however, this compartment likely holds significant amounts of microplastics. By representatively sampling the entire Rhine River cross-section near Basel through five sampling points over 22 months, we found a median microplastic (50-3000 μm) concentration of 4.48 n m-3, and estimated a widely ranging load between 4.04 × 102 n s-1 and 3.57 × 105 n s-1. We also show that the microplastic concentration in the water column was not well explained by river discharge. This suggests that although high discharge events as observed here can over short time periods lead to peak microplastic concentrations (e.g., 1.23 × 102 n m-3), microplastic load variance was not dominated by discharge in the study area.

Cytomolecular diversity among Vigna Savi (Leguminosae) subgenera.

The genus Vigna (Leguminosae) comprises about 150 species grouped into five subgenera. The present study aimed to improve the understanding of karyotype diversity and evolution in Vigna, using new and previously published data through different cytogenetic and DNA content approaches. In the Vigna subgenera, we observed a random distribution of rDNA patterns. The 35S rDNA varied in position, from terminal to proximal, and in number, ranging from one (V. aconitifolia, V. subg. Ceratotropis) to seven pairs (V. unguiculata subsp. unguiculata, V. subg. Vigna). On the other hand, the number of 5S rDNA was conserved (one or two pairs), except for V. radiata (V. subg. Ceratotropis), which had three pairs. Genome size was relatively conserved within the genus, ranging from 1C = 0.43 to 0.70pg in V. oblongifolia and V. unguiculata subsp. unguiculata, respectively, both belonging to V. subg. Vigna. However, we observed a positive correlation between DNA content and the number of 35S rDNA sites. In addition, data from chromosome-specific BAC-FISH suggest that the ancestral 35S rDNA locus is conserved on chromosome 6 within Vigna. Considering the rapid diversification in the number and position of rDNA sites, such conservation is surprising and suggests that additional sites may have spread out from this ancestral locus.

Optimization method of water-quality observation positions in offshore CCS sites using the adjoint marginal sensitivity method

Carbon dioxide (CO2) Capture and Storge (CCS) is recognized as a promising method to mitigate the global warming. In Japan, sub-seabed CO2 storage is considered to be commercialized in a decade. However, offshore CO2 storage has a risk of leakage. Since CO2 seepage into seawater may affect the marine environment, CO2 concentration must be monitored in CO2 storage sea sites under a Japanese law. However, there is no scientifically logical base to determine observation positions. In this study, we therefore developed a method of determining optimal observation positions in CO2 storage sea sites. First, we defined optimal observation positions as those that can estimate seepage positions with high accuracy with the minimal number using the adjoint marginal sensitivity method, even if the seepage positions locate anywhere in a target sea site. Second, non-dimensional parameters were proposed to determine whether a datum at a candidate observation position can be successfully used to estimate a possible seepage position using the adjoint method. Then, we defined a coverage index that indicates how much of a target sea site is “covered” in total by observation points and proposed an algorithm to optimally place observation positions. Finally, we validated the developed method numerically and indicated that, to perform highly accurate estimation, it is desirable that a seepage position be covered by at least three observation points.

An investigation into the flexural properties of sawn timber and poles of South African grown Pinus maximinoi (H.E.Moore)

Pinus maximinoi is a species that can potentially be deployed commercially to provide structural timber and poles for the South African market. The objective of this study was to investigate the flexural properties of sawn timber and poles of P. maximinoi from young plantations of the Southern Cape. A total of 60 trees and 150 logs for the sawmill study were obtained from two research trials on the Longmore estate in the Humansdorp area (13.7 years old), and on the Kruisfontein estate close to Knysna (9.0 years old). Some 74 poles from the same compartments were removed and tested in bending. The P. maximinoi sawn timber had excellent bending properties, superior to sawn timber from other South African Pinus species, tested at similar radial positions in previous studies. Analysis of variation showed that site, log height and board radial position had a significant influence on the modulus of elasticity (MOE) of sawn timber. Multiple regression analysis showed that board MOE can be predicted using a model including tree taper, time-of-flight measured with Treesonic and wood density (model R 2 = 0.87). The P. maximinoi poles complied with the mean MOE national standard requirement SANS 457-2 of 9 000 MPa but not with the fifth percentile (bending strength) modulus of rupture (MOR) requirement of 34 MPa. This study and other research, however, showed that the national standard requirement for MOR of poles might be too high for current log resources in South Africa. The investigation into the bending and other properties of P. maximinoi sawn boards and poles showed extremely positive results. Based on this wood quality study of P. maximinoi, it is certainly a very promising species for deployment in the Southern Cape and potentially other regions in South Africa.

Synthesis of Copper-Substituted Polyoxovanadate and Oxidation of 1-Phenyl Ethanol

Dicopper-substituted polyoxovanadate [Cu2V16O44(NO3)]5− (Cu2V16) was synthesized through the reaction of [Cu2V8O24]4− and [V4O12]4− in the presence of nitrate salt. From single crystal X-ray analysis, Cu2V16 exhibited the same helical structure as that of nitrate-incorporated polyoxovanadate, [V18O46(NO3)]5− (V18). Both complexes had the same framework with the same guest anion and are considered to be substituted isomers for each other by replacing two Cu2+ ions and two [VO]2+ ions. The incorporated nitrate showed short and long N–O bond lengths (1.14, 1.26 and 1.30 Å) as in the case of V18 (1.09, 1.16 and 1.28 Å). Reflecting the inequivalent bond lengths of the nitrate, the IR spectrum of V18 shows split peaks at 1359 and 1342 cm−1. But the Cu2V16 spectrum showed a single peak due to the presence of nitrate at 1353 cm−1. When the temperature was lowered, the nitrate peak in Cu2V16 was split into two positions at 1354 and 1345 cm−1 when the temperature reached −140 °C. These results indicate that the nitrate incorporated in Cu2V16 rotates relatively easily in the Cu2V16 cavity at room temperature compared to V18. In addition, the oxidation of 1-phenyl ethanol to acetophenone with Cu2V16 smoothly proceeded in comparison with V18. By taking advantage of the same framework in both catalysts, we can deduce the position of potential active sites in the oxidation reaction. We have concluded that the most active site is not on the peripheral of the vanadate framework, but it is reasonable to suggest that the active site is on the substituted copper atoms rather than the polyoxovanadate framework.

Inspection of reinforced concrete structures using ground penetrating radar: Experimental approach

This study aims to the practical detection of sub-meter scale voids located inside reinforced concrete structures with different diameters and depths using different frequency antennas of 800, 1000, 1200, and 1600 MHz. The experiment was performed on two concrete blocks with medium-sized voids between 70 and 100 mm diameter, and small-sized voids between 10 and 25 mm diameter. A GPR survey was conducted by dividing each block into two grids of profiles with 50- and 100-mm profile spacing. Then, GPR data was collected along both the horizontal and vertical directions of each block with multi-frequency antennas. The gathered data was processed in 2D and 3D to detect the exact location, dimension, and brightness of these voids. The results showed that GPR has the potential to be quite effective in automating the identification and location of embedded voids within concrete blocks. The accuracy with which the system was able to identify void locations depended mainly on the frequency of the antenna used and the diameter of the void, while the depth of penetration was inversely proportional to the frequency of the used antenna, with estimated depths ranging from 25 cm (using a 1600 MHz antenna) to 1.5 m (using 800 MHz antenna). Moreover, a GPR survey was conducted to evaluate two sites inside residential buildings before and after the rehabilitation process. The radar scan exhibited a notable proficiency in identifying the positions of rebar sites, whether they were situated at a single level or two levels, inside certain areas of construction. The study revealed the ability of GPR to identify the depth of the reinforcing steel within the cement material, particularly in areas where the soil has experienced subsidence.

Place and avoiding the race to the bottom of the fractured well

PurposeThis paper aims to consider the potential implications of the layering of regulation in relation to hydraulic fracturing (fracking) at the borders between the nations of the UK.Design/methodology/approachThis paper uses a qualitative research method grounded in particular in legal geography to examine the existing approaches to regulating hydraulic fracturing and identify the places and their features that are constructed as a result of their intersection at the borders of the nations comprising the UK.FindingsThe current regulatory framework concerning hydraulic fracturing risks restricts the places in which the practice can occur in such a manner as to potentially cause greater environmental harm should the process be used. The regulations governing the process are not aligned in relation to the surface and subsurface aspects of the process to enable their management, once operational, as a singularly constructed place of extraction. Strong regulation at the surface can have the effect of influencing placement of the site only in relation to the place at which the resource sought reaches the surface, whilst having little to no impact on the environmental harms, which will result at the subsurface or relative to other potential surface site positions, and potentially even increasing them.Research limitations/implicationsThis paper is limited by uncertainty as to the future use of hydraulic fracturing to extract oil and gas within the UK. The issues raised within it would also be applicable to other extractive industries where a surface site might be placed within a radius of the subsurface point of extraction, rather than having to be located at a fixed point relative to that in the subsurface. This paper therefore raises concerns that might be explored more generally in relation to the regulation of the place of resource extraction, particularly at legal borders between jurisdictions, and the impact of regulation, which does not account for the misalignment of regulation of spaces above and below the surface that form a single place at which extraction occurs.Social implicationsThis paper considers the potential impacts of misaligned positions held by nations in the UK in relation to environmentally harmful practices undertaken by extractive industries, which are highlighted by an analysis of the extant regulatory framework for hydraulic fracturing.Originality/valueWhilst the potential for cross internal border extraction of gas within the UK via hydraulic fracturing and the regulatory consequences of this has been highlighted in academic literature, this paper examines the implications of regulation for the least environmentally harmful placement of the process.

Eucalyptus productive matrix influences the soil seed bank in southern Brazil

&lt;i&gt;Eucalyptus &lt;/i&gt;species are cultivated for various purposes around the world, mainly because they adapt well and grow quickly. These productive matrices of planted forests are common in Brazil, added to which, areas of native vegetation are requirements of environmental legislation. Although eucalyptus plantations are widely distributed in Brazil, little is known about the soil seed bank (SSB) in the eucalyptus productive matrix (EPM). We aim to understand how the EPM made up of eucalyptus plantations and native forest remnants affect the SSB in the Brazilian Pampa. Samples of the SSB of EPM were collected and monitored for six months in a shade house. We evaluated seedling emergence, richness, composition, ecological characteristics of the species, diversity, and floristic similarity. We recorded a high rate of seedling emergence, species richness, and different life forms and native species in all EPM treatments, however the similarity between native remnants and eucalyptus plantations was low. The size and level of conservation of native remnants and the position and management of eucalyptus sites in the EPM influenced the diversity and composition of species. Eucalyptus plantations contain SSBs with potential for natural regeneration when they are in a landscape that maintains conserved native forest remnants. These results expand the knowledge of the SSB in EPM and can support actions in ecological restoration projects.

Application of ultrasound-guided medical thoracoscopy in patients with small amounts or without pleural effusion

BackgroundPleural disease is a common clinical condition, and some patients present with a small amount of pleural effusion or no pleural effusion. It is difficult to diagnose such patients in clinical practice. Medical thoracoscopy is the gold standard for the diagnosis of pleural effusion with unknown origin, and guidelines recommend that pneumothorax should be induced in such patients before medical thoracoscopy examination. However, the process of inducing pneumothorax is tedious and has many complications. Our study was conducted to clarify the value of thoracic ultrasound combined with medical thoracoscopy in patients with small amounts or without pleural effusion to simplify the process of medical thoracoscopy examination.MethodsIn this retrospective study, we included patients who were assigned to complete medical thoracoscopy. Successful completion of medical thoracoscopy in patients was regarded as letting the endoscope get into the pleural cavity and completion of the biopsy. Finally, we analyzed the value of preoperative ultrasound in patients without or with small amounts of pleural effusion.ResultsSeventy-two patients were finally included in the study. Among them, 68 patients who underwent ultrasound positioning of the access site successfully completed the examination and four patients failed the examination. Fifty-one cases showed no fluid sonolucent area at the access site, of which 48 cases had pleural sliding signs at the access site, and 47 patients successfully completed the examination; 3 cases without pleural sliding signs at the access site failed to complete thoracoscopy. In 21 cases, the fluid sonolucent area was selected as the access site, and all of them successfully completed thoracoscopy.ConclusionMedical thoracoscopy is one of the methods to confirm the diagnosis in patients with pleural disease with small amounts or without pleural effusion. The application of thoracic ultrasound before medical thoracoscopy can be used for the selection of the access site. It is possible to replace pneumothorax induction before medical thoracoscopy.

Influence of Magnesium Source on the Mechanochemical Synthesis of Magnesium-Substituted Hydroxyapatite.

Magnesium, as one of the most abundant cations in the human body, plays an important role in both physiological and pathological processes. In this study, it was shown that a promising biomedical material, Mg-substituted hydroxyapatite (Mg-HA), can be synthesized via a fast mechanochemical method. For this method, the nature of magnesium-containing carriers was shown to be important. When using magnesium oxide as a source of magnesium, the partial insertion of magnesium cations into the apatite structure occurs. In contrast, when magnesium hydroxide or monomagnesium phosphate is used, single-phase Mg-HA is formed. Both experimental and theoretical investigations showed that an increase in the Mg content leads to a decrease in the lattice parameters and unit cell volume of Mg-HA. Density functional theory calculations showed the high sensitivity of the lattice parameters to the crystallographic position of the calcium site substituted by magnesium. It was shown experimentally that the insertion of magnesium cations decreases the thermal stability of hydroxyapatite. The thermal decomposition of Mg-HA leads to the formation of a mixture of stoichiometric HA, magnesium oxide, and Mg-substituted tricalcium phosphate phases.

Risk indicators for gingival recession in the esthetic zone: A cross-sectional clinical, tomographic, and ultrasonographic study.

To evaluate the risk indicators associated with midfacial gingival recessions (GR) in the natural dentition esthetic regions. Cone-beam computed tomography (CBCT) results of thirty-seven subjects presenting with 268 eligible teeth were included in the cross-sectional study. Clinical measurements included presence/absence of midfacial GR; the depth of the midfacial, mesial, and distal gingival recession; the recession type (RT); keratinized tissue width (KT); and attached gingiva width (AG). Questionnaires were utilized to capture patient-reported esthetics and dental hypersensitivity for each study tooth. Buccal bone dehiscence (cBBD) and buccal bone thickness (cBBT) were measured on the CBCT scans. High-frequency ultrasonography was performed to assess gingival thickness (GT) and buccal bone dehiscence (uBBD). Intraoral optical scanning was obtained to quantify the buccolingual position of each study site (3D profile analysis). Multilevel logistic regression analyses with generalized estimation equations were performed to assess the factors associated with the conditions of interest. The presence of midfacial GR was significantly associated with the history of periodontal treatment for pocket reduction (OR 7.99, p=0.006), KT (OR 0.62, p<0.001), cBBD (OR 2.30, p=0.015), GT 1.5mm from the gingival margin (OR 0.18, p=0.04) and 3D profile 1mm from the gingival margin (OR 1.04, p=0.001). The depth of midfacial GR was significantly correlated to previous history of periodontal treatment (OR 0.96, p=0.001), KT (OR -0.18, p<0.001), presence of bone fenestration (OR 0.24, p=0.044), and cBBD (OR 0.43, p<0.001). The depth of midfacial GR was also the only factor associated with patient-reported esthetics (OR -3.38, p=0.022), while KT (OR 0.77, p=0.018) and AG (OR 0.82, p=0.047) were significantly correlated with patient-reported dental hypersensitivity. Several risk indicators of midfacial and interproximal GR in the esthetic region were identified. The use of imaging technologies allowed for detection of parameters associated with the conditions of interest, and, therefore, their incorporation in future clinical studies is advocated. Ultrasonography could be preferred over CBCT for a noninvasive assessment of periodontal phenotype.