Natural fibers has recently become one of the most crucial material available in the environment. Natural fiber has gained popularity due to its light weight, greater specific stiffness, low cost, easily available, and longer fatigue life. It has high-specific properties such as impact resistance, low density, flexibility, biodegradability, and economy are exhibited by these natural fibers. The other material used in robot include issues like temperature sensitivity in plastic, weight, and corrosion in iron metal, and composites. The main aim of this paper is to test how natural fiber material is best suitable to use for building a chassis of pesticide spraying robot. This work focuses on test specimens made by hand layup technique from a mixture of natural fibers from sisal wood and epoxy resin production procedure. Tests conducted on a 50/50 fraction of these materials show encouraging results 40.13 Mpa for tensile strength, 24.26 N/mm2 for compression strength, and 0.0384 for the Izod Impact test result. Additionally, a 30–70 percentage shows an Izod Impact test result of 0.0239, a tensile strength of 65.66 Mpa, and a compression strength of 21.86 N/mm2. The result showed that natural fiber is the most appropriate material used in pesticide spraying robot structure. Sisal natural fiber material has potential for use in a variety of robotic applications, especially in fields that require materials that are ecologically benign, biodegradable, and lightweight. By including more possible natural fibers, altering the fiber's composition and orientation, and analyzing its mechanical and machining properties, the experiments can be expanded.