Single Water Research Articles

Novel Method on Mixing Degree Quantification of Mine Water Sources: A Case Study

After a mine water inrush occurs, it is crucial to quickly identify the source of the water inrush and the key control area, and to formulate accurately efficient water control measures. According to the differences in water chemical characteristics of four aquifers in the Fenyuan coal mine, the concentrations of K+~Na+, Ca2+, Mg2+, Cl−, SO42−, and HCO3− were taken as water source identification indexes. A decision tree classification model based on the C4.5 algorithm was adopted to visualize the chemical characteristics of a single water source and extract rules, and intuitively obtained the discrimination conditions of a single water source with Mg2+, Ca2+, and Cl− as important variables in the decision tree: Mg2+ < 39.585 mg/L, Cl− < 516.338 mg/L and Mg2+ ≥ 39.585 mg/L, Ca2+ < 160.860 mg/L. Factor analysis and Fisher discriminant theory were used to eliminate the redundant ion variables, and the discriminant function equations of the two, three, and four types of mixed water sources were obtained successively in turn. This paper puts forward MSE, RMSE, and MAE as the evaluation indexes of the water source mixing degree calculation models and obtains the ranking of the pros and cons of the mixed water source mixing degree calculation models. The results show that the minimum inscribed circle analytical method is the optimal model for the calculation of the mixing degree of two types of water sources, and the MSE, RMSE, and MAE are 0.17%, 4.13%, and 4.13%, respectively. The minimum inscribed circle clustering method is the optimal model for the calculation of the mixing degree of three types of water sources, and the minimum distance method is the optimal model for the calculation of the mixing degree of four types of water sources. The method of mine water source identification based on the decision tree C4.5 algorithm and mixing degree calculation has the characteristics of a simple calculation process, high efficiency, objective accuracy, and low cost, which can provide a scientific basis for the development of stope water control measures.

Experimental study and model optimization of thermodynamic performance of a single screw water vapor compressor

Single screw water vapor compressor has greater advantages under conditions of low suction temperature and high compression ratio, however, the current study has the following limitations: (1) the volumetric and isentropic efficiency were substituted as a fixed value into the models of compressor, (2) The suction temperature is concentrated in the medium-high region, (3) The compression ratio is concentrated in the medium-low range, which failed to accurately describe the water vapor compression process. Therefore, the relationship between the volumetric efficiency, isentropic efficiency and key characteristic parameters of compressor is studied experimentally, the mathematical models of the volume flow and compression power are established and verified by experiments, the changes of the mass flow, compression power, COP and SMER with suction temperature, discharge pressure and compression ratio are analyzed, the operation process parameters of the single-screw water vapor compressor are optimized.

Exploring the influence of interfacial solvation on electrochemical CO2 reduction using plasmon‐enhanced vibrational sum frequency generation spectroscopy

AbstractAlthough interfacial solvation plays an important role in determining carbon dioxide reduction (CO2R) kinetics, present understanding of the potential dependent properties of the electrochemical double layer under conditions relevant for CO2R remains limited. This article summarizes the development and recent applications of plasmon‐enhanced vibrational sum frequency generation (VSFG) spectroscopy to study the effects of cation hydration and interfacial solvation on CO2R using CO as a vibrational Stark reporter. Results show that electrolyte cations retain their entire solvation shell upon adsorption to inactive sites, while active sites retain only a single water layer between the gold surface and the cation. Measurements also show that the total interfacial electric field can be separated into two contributions: one from the electrochemical double layer (Stern field) and another from the polar solvation environment (Onsager field). Surprisingly, correlating VSFG spectra with reaction kinetics reveals that it is the solvation‐mediated Onsager field that governs the chemical reactivity at the electrode/electrolyte interface. Measuring the interfacial water spectra during electrocatalysis also provides evidence for the proton source during H2 evolution, which competes with CO2R in aqueous electrolyte. These findings highlight the importance of directly probing cation hydration and interfacial solvation, which mediates reaction kinetics at electrochemical interfaces.

Numerical simulation of water wave propagation using DualSPHysics

In this study, the study of water waves was carried out using one of major the mesh-free CFD i.e. Smoothed Particle Hydrodynamics (SPH). DualSPHysics ver 5.0 which is an open-source SPH solver based on Weakly Compressible Smoothed Particle Hydrodynamics (WCSPH) was used to reproduce three dimension of water waves in large and medium numerical wave tanks (NWT). This study analyses water wave profiles and the accuracy of DualSPHysics for regular waves with period 1.15 second and two NWT were used, i.e., 35 and 15 m. In addition, advanced post-processing using VisualSPHysics was used to mimic water waves in real conditions. Single water depth was used, i.e. 0.4 meters for large and medium NWT. The results showed SPH has limitations for reproducing large NWT. However medium NWT successfully reproduce water wave with advanced post-processing using VisualSPHysics.

Bubble entrainment and underwater noise caused by a single water drop falling on the surface of freshwater and saltwater

Bubble entrainment caused by and underwater sound produced by a single water drop falling on the surface of freshwater and saltwater were experimentally investigated in this study. A charge-coupled device camera was employed to photograph the drop's impact and the process of bubble formation. Underwater sound was received using a hydrophone connected to a charge amplifier and a data-acquisition system. To capture the temporal and spectral characteristics of the sound, the time series of the underwater sound was transformed into the time–frequency domain by using the Gabor transform. The diameter of the drops was varied from 2.6 to 5.66 mm, and the release heights investigated were 10–150 cm. The experimental results revealed three distinct bubble formation processes following the impact of a water drop. Type-I bubbling was the formation of a singular bubble at the apex of the contracting crater produced by the initial drop impact. Type-II bubbling was the formation of one or multiple bubbles entrained by the downward motion of the water column produced during the initial drop impact. Type-III bubbling was the formation of a singular bubble generated due to the impact of a secondary water drop above the water column. The effects of salinity on bubble entrainment and underwater noise were also studied. For the same experimental parameters, bubbles entrained in saltwater were discovered to be larger than those entrapped in freshwater; moreover, the saltwater bubbles collapsed into two bubbles.

(+)-Cedrol hemihydrate: a natural product derived from drying eastern red cedar (Juniperus virginiana) wood.

Cedrol-like compounds are of pharmacological interest due to their diverse range of medicinal effects and are used globally in traditional medicines and cosmetics. Many cedrol tautomers are known from molecular studies but few have been studied in crystalline form by X-ray diffraction. Acicular white crystals collected from the wood of eastern red cedar (Juniperus virginiana) are determined to be (+)-cedrol hemihydrate, namely, (1S,2R,5S,7R,8R)-2,6,6,8-tetramethyltricyclo[5.3.1.01,5]undecan-8-ol hemihydrate, C15H26O·0.5H2O, a novel packing of two unique cedrol molecules (Z' = 2) with a single water molecule [space group P212121; a = 6.1956 (1), b = 14.5363 (1), and c = 30.9294 (4) Å]. The hydrogen bonding forms a one-dimensional spiral chain running along the a axis, following the chirality of the cedrol molecule, through hydrogen-bonding interactions with a right-handed helical configuration in graph-set notation Δ-C33(6) > a > c > b. The crystal packing and symmetry are different from crystalline isocedrol due to the different hydrogen-bonding geometry.

Designing a Single Slope Solar Water Still and Testing it in Omani Climate

Oman has a limited resources of fresh water mainly underground water. To preserve those resources and to meet the increase in the fresh water demand, the country turned to water desalination which is known to be very costly and harmful to the environment. Water desalination using solar energy is an attractive alternative for countries like Oman which has a sunny weather all year round and where the length of the day time ranges between 11 to 14 hours. Furthermore, solar energy is a clean source of energy. The aim of the present study is to (1) design and test the solar water still for Omani climate, and (2) to investigate the effect of different design parameters on the still thermal performance.

Analysis of particle dispersion and cavity formation during bulk particle water entry using fully coupled CFD-DEM-VOF approach

This paper presents the development and validation of a fully coupled computational fluid dynamics—discrete element method—volume of fluid (CFD-DEM-VOF) model to simulate the complex behavior of particle-laden flows with free surfaces. The coupling between the fluid and particle phases is established through the implemented continuity, momentum, and alpha transport equation. The coupled particle forces such as drag, pressure gradient, dense virtual mass, viscous, and interface forces are also integrated, with drag and dense virtual mass forces being dependent on local porosity. The integrated conservative alpha transport equation ensures phase volume conservation during interactions between particles and water. Additionally, we have implemented a trilinear interpolation method designed to operate on unstructured hexahedral meshes. This method has been tested for its ability to properly resolve the coupling effects in the numerical simulations, particularly in cases with a relatively low cell-size ratio. The model is validated through three distinct test cases: single particle water entry, dam break with particles, and water entry of a group of particles case. The experimental setup is built to study the dynamics of the water entry of a group of particles, where three key flow features are analyzed: the evolution of average particle velocity, cavity shape, and particle dispersion cloud profiles in water. The tests involve four different scenarios, including two different water levels (16.1 and 20.1 cm) and two different particle densities (2650 and 4000 kg/m3). High-speed videometry and particle tracking velocimetry (using ImageJ/TrackMate) methods are employed for experimental data acquisition. It is demonstrated that numerical results are in excellent agreement with theoretical predictions and experimental data. The study highlights the significance of vortices in cavity shaping and particle dispersion. The validated CFD-DEM-VOF model constitutes a robust tool for simulating particle-laden flows, contributing valuable insights into the complex interplay between particles and fluids.

Optimization with genetic algorythms of PVT system global efficiency

Photovoltaic (PV) solar panels generally produce electricity in the 6% to 12% efficiency range, the rest being dissipated in thermal losses. To recover this amount, hybrid photovoltaic thermal systems (PV/T) have been devised. These are devices that simultaneously convert solar energy into electricity and heat. A significant amount of research on PV/T collectors has been carried out over the last decade and water PV/T glazed flat plate collector systems turned out to be the most promising to develop (Zondag[1]). It is thus interesting to study the PV/T system as part of a closed loop single phase water CDU (coolant distribution unit) in laminar forced convection. In particular, the analysis was conducted on the optimal cooling performance of the thermal part, testing polynomial channel profiles of varying order (from zero to fourth) for channels of a real industrial module heat sink, under the following conditions: ideal flux of 1000 W/m² on one side, insulation on the opposite side, periodic conditions on the remaining sides, fully developed thermal and velocity profile in laminar flow of water. Through the use of a genetic algorithm, we have optimized the shape of the channel’s sidewalls in terms of heat transfer maximization. In terms of Nusselt number, results show that fourth order profiles are the most efficient. When limits to allowable pressure loss and module weight are introduced, these bring generally to a lower efficiency of the system than the unconstrained case.

Land use characteristics affect the sub-basinal scale urban fish community identified by environmental DNA metabarcoding

The heterogeneity of urban landscapes has effects on the environmental characteristics and fish composition of individual urban streams, even within a single water system. It is, therefore, imperative to assess the influence of physiochemical properties on urban streams by analyzing the spatial distribution of fish communities at the local scale. However, conventional fish surveys encounter time and labor constraints when selecting and surveying dense sampling points under 2 km in stream networks. In this study, environmental DNA (eDNA) metabarcoding was used as an innovative survey methodology to identify the effects of land use and stream order on fish composition and tolerance guild in an urban area. The eDNA sampling was conducted in 31 sites of the Anyang stream network in Korea, including part of the stream undergoing ecological restoration. The eDNA survey detected 12 of 17 species (70.6%) that appeared in the historical data, and 12 of 18 species (66.7%) identified in a conventional field survey with kick nets and casting nets. The proportions of urban area, forest and grassland were positively correlated with abundance (p < 0.05) and richness (p < 0.05) in multiple regression analyses, while the proportion of agricultural area showed a negative correlation (p < 0.05). For abundance, richness, and diversity within the fish community from first- to third-order streams, there was a significant decrease in sensitive species (p < 0.05) alongside a significant increase in tolerant species (p < 0.01) across all three indices. The results of this study highlight variations in fish composition across sites within the local scale of the urban stream network, underscoring the need for detailed monitoring to understand the ecological function of urban streams.

Increased precipitation alters the effects of nitrogen deposition on soil bacterial and fungal communities in a temperate forest

Anthropogenic nitrogen (N) deposition and increased precipitation are known to alter soil microbial communities. However, the combined effects of elevated N deposition and increased precipitation on soil microbial community dynamics and co-occurrence networks in temperate forests remain elusive. In this study, we conducted a field manipulation experiment by applying N solution and water to the forest canopy to simulate natural N deposition and increased precipitation in a temperate forest. We collected samples in the litter layer, organic soil layer, and mineral soil layer in 2018–2019 after 6–7 years of N and water treatments, and explored how elevated N deposition and increased precipitation regulate soil microbial diversity, community composition, and co-occurrence networks in different soil layers and at different sampling times. We found that the effects of N deposition and increased precipitation on soil microbial communities varied with soil layers and sampling times. Compared to the ambient environment, single canopy N addition (CN) or single canopy water addition (CW) did not affect bacterial Shannon diversity in the mineral soil layer in 2018, but the combined canopy N and water additions (CNW) decreased it in this layer at this time. CN increased fungal OTU richness in the organic and mineral soil layers in 2018; however, CW and CNW did not have an effect on it in the same layer at the same time. CW and CNW, but not CN, significantly affected bacterial and fungal community compositions in the litter layer in 2018 and in the organic soil layer in 2019. In contrast, CN, but not CW or CNW, significantly affected fungal community composition in the litter layer in 2019. CNW exhibited higher complexities of bacterial and fungal co-occurrence networks than CN and the ambient environment, indicating increased precipitation can strengthen the effect of N deposition on the complexity of bacterial and fungal co-occurrence networks. Our findings suggest that increased precipitation alters the effects of atmospheric N deposition on soil bacterial and fungal communities in this temperate forest, depending on soil layer and sampling time. Moreover, both bacterial and fungal community compositions are sensitive to increased precipitation, but the bacterial community composition is more sensitive to N deposition than the fungal community composition in the organic and mineral soil layers in this forest.

Prospective Health Outcomes of Sugar-Sweetened Beverage Consumption Patterns Associated with Sociodemographic and Ethnic Factors among Chinese Adults

Background: The consumption rate of artificially sweetened beverages, 100% fruit juices, and soft drink products has been proliferating since the 1980s among the Chinese population. Sugar-sweetened beverages (SSBs) prevalence and high intake of SSBs are associated with several noncommunicable chronic diseases (NCDs). This trend presents a serious public health threat to the Chinese population. Purpose: The present cross-sectional study examined the association between added sugar-sweetened beverage consumption patterns and prospective adverse health outcomes among Chinese sociodemographic and ethnic groups. Methods: The study population of 662 male and female adults partook in the study. The study cohort was randomly drawn from four Chinese cities in three provinces. Participants responded to an adapted cross-sectional self-administered paper-and-pencil 24-hour dietary intake recall protocol questionnaire, including 23 closed-ended questions on the type and frequency of the beverage consumed, sociodemographic characteristics, and ethnic group affiliation. A t-test was conducted to analyze the interactions between the parametric variables and Chi-square analyses were performed to determine interactions between the nonparametric variables. Results: The consumption frequency of sugar-sweetened beverages at least once/day was examined. For all respondents, water consumption was employed as a benchmark for a healthy beverage compared to all other sugar-sweetened beverage consumption. Findings suggest that water was the most frequently consumed beverage with the highest rate of intake (53.17%) compared to sugar-sweetened beverages consumption Regarding ethnicity, Han's water consumption was reported at (67.94%) compared to sugar-sweetened beverage consumption combined at (32.06%). Concerning Huis, water consumption was (60.56%) contrasted with sugar-sweetened beverage consumption of (39.44%). Hans were more likely to consume both water and sugar-sweetened beverages compared to Hui participants. Males' water consumption rate was at (66.74%) juxtaposed with sugar-sweetened beverage consumption of (36.26%). Females' water consumption rate was (66.51%) contrasted with a sugar-sweetened beverage consumption rate of (39.44%). There were no significant differences in the water and sugar-sweetened beverages between men and women. Married respondents' water consumption was (65.44%) compared to sugar-sweetened beverage consumption rate (34.55%). Regarding marital status, the single respondents' water consumption was (64.89%) compared to sugar-sweetened beverage consumption of (33.49%). There were no significant differences in water and sugar-sweetened beverage consumption between the marital status groups. Conclusions: There is a need to introduce short-term and long-term strategies to curtail sugar-sweetened beverage consumption through excise taxes, making water more readily accessible in public schools, and launching a public health education campaign aimed at the health risks associated with added sugar-sweetened beverage consumption.

Effect of intra-seasonal variation in precipitation on seed germination and seedling growth in Iris atrofusca: implications for conservation

Abstract Understanding the modulating effect of environmental conditions on the germination of seeds constituting a persistent soil seed bank has both theoretical and practical merits. I studied the role of precipitation in the germination of seeds of the imperiled geophyte Iris atrofusca, which exhibits strong seed dormancy and intermittant germination over years. I wanted to know how a single significant rain event in one of the first several seasons after seed maturation and dispersal can affect seed germination and seedling growth in I. atrofusca. This is important because knowing the amount and time of such a rainfall event, one can imitate it by a single watering applied to an artificial soil seed bank, thus initiating or substantially increasing seed germination and improving seedling growth and therefore survival. Overall, the results imply that the most efficient in terms of both seed germination and seedling survival is supplementary irrigation equivalent to 40 mm rainfall applied as a single rain event to the artificial soil seed bank early in the first and second season, plus watering equivalent to 20–40 mm in the months when the amount of precipitation is low (below 20 mm) not only in the first and second, but also in subsequent seasons.

The reaction mechanism of SO3 with the multifunctional compound ethanolamine and its atmospheric implications.

SO3 is an important reactive species in sulfur cycle and sulfuric acid formation processes and its reactions with some functional group substances, such as H2O, NH3, CH3OH, and organic and inorganic acids, have been extensively studied. However, its loss mechanism with multifunctional species is still lacking in detail. Herein, the reaction mechanism between SO3 and monoethanolamide (MEA) was investigated in the gas phase and on water droplets. The quantum chemical calculations indicate that the gas-phase reactions of SO3 with the OH and NH2 moieties of MEA hardly occur as their reaction energy barriers are up to 21.9-29.4 kcal mol-1. When a single water molecule is added into the SO3 + MEA reaction, it not only decreases the reaction barrier by at least 15.0 kcal mol-1 and thus enhances the rate obviously, but can also lead to the main product changing from HOCH2CH2NHSO3H to NH2CH2CH2OSO3H. The Born Oppenheimer molecular dynamics simulations on a water droplet show that the routes of the NH2CH2CH2OSO3-⋯H3O+ ion pair, HSO4- and HOCH2CH2NH3+ ions and zwitterionic formations of HOCH2CH2NH2+-SO3- and SO3--OCH2CH2NH3+ occur through a loop-structure route or chain reaction process, and can be finished within several picoseconds. Interestingly, the nucleation simulations show that the products of HOCH2CH2NHSO3H and NH2CH2CH2OSO3H have a potential ability to participate in the formation of new particles as they can form larger clusters with H2SO4, NH3 and H2O molecules within 20 ns. Thus, the present study will not only give new insight into the reaction between SO3 and multifunctional compounds, but also provide a new potential formation mechanism for particles resulting from the loss of SO3.

Experimental analysis of water-droplet–fiber interaction on a mechanically excited hydrophobic fiber

In this study, the dynamics of a single water droplet on a mechanically excited single fiber are investigated fundamentally. By utilizing state-of-the-art high-speed camera technology, the droplet's motion is captured with exceptional temporal resolution, enabling a detailed analysis of its position, size, and kinetics. We can identify distinct motion patterns of a droplet adhering to the fiber, which can exhibit either a static, a tilting, or swinging motion. The swinging and tilting motion can be overlaid with a higher-frequency deformation in response to the fiber excitation. Additionally, we examine the detachment of the droplet from the fiber as well as for the first time the (periodic) reattachment resulting from the mechanical excitation. The used droplet volumes are smaller, and the excitation shown here is greater than the excitation acceleration previously investigated in single fiber studies. Insights into droplet–fiber interactions can provide a better understanding of the mechanisms occurring in coalescence filters in harsh environments, which cannot be observed in situ with high temporal and spacial resolution in a full-scale filter due to the lack of optical access.

Water is a radiation protection agent for ionised pyrrole.

Radiation-induced damage of biological matter is an ubiquitous problem in nature. The influence of the hydration environment is widely discussed, but its exact role remains elusive. Utilising well defined solvated-molecule aggregates, we experimentally observed a hydrogen-bonded water molecule acting as a radiation protection agent for ionised pyrrole, a prototypical aromatic biomolecule. Pure samples of pyrrole and pyrrole(H2O) were outer-valence ionised and the subsequent damage and relaxation processes were studied. Bare pyrrole ions fragmented through the breaking of C-C or N-C covalent bonds. However, for pyrrole(H2O)+, we observed a strong protection of the pyrrole ring through the dissociative release of neutral water or by transferring an electron or proton across the hydrogen bond. Overall, a single water molecule strongly reduces the fragmentation probability and thus the persistent radiation damage of singly-ionised pyrrole.

M(II) effect on encapsulation of guests into a series of M3L2 chiral cages: enantio-recognition.

Self-assembly of M(ClO4)2 (M2+ = Ni2+, Cu2+, and Zn2+) with (1S,1'S,1''S,2R,2'R,2''R)-(benzenetricarbonyltris(azanediyl))tris(2,3-dihydro-1H-indene-2,1-diyl) trinicotinate (s,r-L) and the corresponding enantiomer (r,s-L) as a pair of chiral tridentate donors gives rise to the chiral cage pairs [M3(s,r- and r,s-L)2](ClO4)6. For the two pairs of [(Me2CO)(H2O)@M3(r,-s and s,r-L)2](ClO4)6 (M2+ = Ni2+ and Zn2+), the inner cavity is occupied by both an acetone and a single water molecule, whereas for the copper(II) pair of [Me2CO@Cu3(r,s- and s,r-L)2](ClO4)6 under the same conditions, the cavity is filled by only one acetone molecule. Thus, the encapsulation of guest molecules into the cages during self-assembly shows significant metal(II) ion effects. These chiral cages are effective for the enantio-recognition of chiral (S)-2-butanol and (R)-2-butanol via the shifts of the electrochemical oxidation potentials obtained by the linear sweep voltammetry (LSV) technique, density functional theory (DFT) calculations, and the chiral 2-butanol adsorption in the single-crystal-to-single-crystal (SCSC) mode.

Adsorption kinetics of H2O on graphene surface based on a new potential energy surface

The interaction between water and graphene is important for understanding the thermodynamic and kinetic properties of water on hydrophobic surfaces. In this study, we constructed a high-dimensional potential energy surface (PES) for the water-graphene system using the many-body expansion scheme and neural network fitting. By analyzing the landscape of the PES, we found that the water molecule exhibits a weak physisorption behavior with a binding energy of about − 1000 cm−1 and a very low diffusion barrier. Furthermore, extensive molecular dynamics were performed to investigate the adsorption and diffusion dynamics of a single water on a graphene surface at temperatures ranging from 50 to 300 K. Potential-of-mean-forces were computed from the trajectories, providing a comprehensive and accurate description of the water-graphene interaction kinetics.

Vibrational circular dichroism spectra of proline in water at different pH values.

Recording VCD spectra of aqueous solution poses a particular challenge as water is a strong infrared absorber. Likewise, the computational analysis of VCD spectra by means of DFT-based spectral calculations requires the consideration of explicit solvent molecules, thus posing an even greater challenge. Several studies suggested that by modeling the solvent environment with a few water molecules in a micro-solvation approach would be sufficient to describe experimental spectra. For example, using proline at different pH values, we herein show that a change in the relative spatial orientation of a single water molecule in five-fold solvated structures strongly affects the computed VCD spectral signatures and that Boltzmann-weighted spectra do not correctly reproduce the experiment. We thus explored an approach based on molecular dynamics and subsequent DFT-calculations, in which we considered 30 water molecules (about 1.5 solvation shells). Once again, it was found that the Boltzmann-weighted spectra obtained on the basis of several hundred structures did not correctly reproduce experimental signatures, and a simple averaging scheme resulted in well-matching spectra with comparable bandwidths. The rationale behind the procedure was that sampling the configurational space of the solvent molecules is as equally important as the conformational sampling of the solute. For conformationally more flexible molecules, it is assumed that a much larger set of structures will have to be computed in order to properly sample the conformational space of both solute and solvent.

Remote focusing optical tweezers for 3D imaging.

We present a remote focusing optical tweezer utilizing a 4f symmetrical optical system to compensate the high-order aberration during annular light refocusing. The position of the optical trap can be adjusted beyond the range of one hundred micrometers in the axial direction by means of tuning the position of the mirror placed in the focal region of the illumination objective lens. This optical tweezer can be combined with a sectioning microscope to realize three-dimensional (3D) imaging, e.g., a confocal microscope using a single water immersion objective lens. All optical elements are placed in one side of the sample, which is very useful for application in fields such as radiation biology, where radiation or magnetism disturbance must be introduced on the other side of the sample. In the experiment, a 10 µm diameter silicon dioxide microsphere and pollen cells immersed in the water are translated along the axis using the optical tweezer and, meanwhile, the sectioning images are obtained using the confocal microscope.