This study was conducted to investigate the effect of metronome guidance on the performance of infant cardiopulmonary resuscitation (CPR). A total of 36 medical doctors conducted a 2-min single rescuer CPR with the two-finger technique (TFT) or two-thumb encircling hands technique (TT) on an infant manikin without metronome guidance (baseline test). After completing the baseline test, the participants were assigned to either a "guidance group" or "non-guidance group." The guidance group performed CPR with a high-pitched sound at 110 beats/min from a metronome (test 2), while the non-guidance group performed 2-min CPR without metronome guidance (test 1). Comparison between the results of tests 1 and 2 showed that the ratio of adequate chest compression rate was significantly different in both the TFT (73% [34-93] vs. 98% [95-99], P < 0.001) and the TT (53% [32-79] vs. 99% [98-100], P = 0.010). Other parameters including average depth and the ratio of adequate depth were not significantly different between tests 1 and 2 in both the TFT and TT.Conclusion: Metronome guidance improves the adequacy of chest compression rate during infant CPR without affecting chest compression depth in both the TFT and TT.Trial registration: Clinical Research Information Service, KCT0002735 What is Known: • The rate of chest compressions can be optimized by the use of metronome guidance in pediatric cardiopulmonary resuscitation (CPR). • An adverse effect of deteriorating chest compression depth was found while using a metronome guidance during adult CPR simulations. What is New: • The metronome guidance improved the adequacy of the chest compression rate during infant CPR without affecting other parameters including average depth and the ratio of adequate depth in both the two-finger chest compression technique and two-thumb encircling hand technique.