Single-step Process Research Articles

Selective recovery of Co(II), Mn(II), Cu(II), and Ni(II) by multiple step batch treatments with nanocellulose products.

The recovery of Co(II), Mn(II), Ni(II), and Cu(II) from black mass e-waste solutions through cellulose nanofibers (CNFs) and nanocrystals (CNCs) was investigated. These materials were synthetized by TEMPO-oxidation followed by high-pressure homogenization, and acid hydrolysis, respectively. The NC characterization included the measurement of consistency, cationic demand, carboxylic content, dissolved amorphous cellulose, and transmittance at λ = 600nm. These parameters revealed a high transmittance of the NC solutions and a large presence of anionic groups on the surface. The high surface area and charge of the NC justify their high interaction with the cationic metals. Results indicate that short contact times (even 1min) and low sorbent doses (10mg/L) at acidic pHs (2 to 4) implied remarkable sorption capacities in most cases with more than 1g/g of sorption capacity of Co(II), Mn(II), and Cu(II) in single-step sorption tests. Such levels of sorption capacities exceed by at least one order of magnitude most of the literature values of metal recovery applying cellulosic materials. Isotherm modeling through a combination of Langmuir and Freundlich models suggested that both sorption and surface precipitation occurred. A novel procedure following multiple-step batch operation was applied for Mn(II) sorption. This new method was applied as a five-step process, leading to a fourfold and 18-fold increase of sorption capacity onto CNCs and CNFs, respectively, compared to the single-step process. Therefore, this process shows an innovative way to implement the multiple-step batch sorption with NC as an efficient and environmentally friendly solution for critical metal recovery from e-waste leachates.

One step 4× and 12× 3D-ExM enables robust super-resolution microscopy of nanoscale cellular structures.

Super-resolution microscopy has become an indispensable tool across diverse research fields, offering unprecedented insights into biological architectures with nanometer scale resolution. Compared with traditional nanometer-scale imaging methods such as electron microscopy, super-resolution microscopy offers several advantages, including the simultaneous labeling of multiple target biomolecules with high specificity and simpler sample preparation, making it accessible to most researchers. In this study, we introduce two optimized methods of super-resolution imaging: 4-fold and 12-fold 3D-isotropic and preserved Expansion Microscopy (4× and 12× 3D-ExM). 3D-ExM is a straightforward expansion microscopy technique featuring a single-step process, providing robust and reproducible 3D isotropic expansion for both 2D and 3D cell culture models. With standard confocal microscopy, 12× 3D-ExM achieves a lateral resolution of <30 nm, enabling the visualization of nanoscale structures, including chromosomes, kinetochores, nuclear pore complexes, and Epstein-Barr virus particles. These results demonstrate that 3D-ExM provides cost-effective and user-friendly super-resolution microscopy, making it highly suitable for a wide range of cell biology research, including studies on cellular and chromatin architectures.

Flatness constraints in the estimation of GNSS satellite antenna phase center offsets and variations

Accurate information on satellite antenna phase center offsets (PCOs) and phase variations (PVs) is indispensable for high-precision geodetic applications. In the absence of consistent pre-flight calibrations, satellite antenna PCOs and PVs of global navigation satellite systems are commonly estimated based on observations from a global network, constraining the scale to a given reference frame. As part of this estimation, flatness and zero-mean conditions need to be applied to unambiguously separate PCOs, PVs, and constant phase ambiguities. Within this study, we analytically investigate the impact of different boresight-angle-dependent weighting functions for PV minimization, and we compare antenna models generated with different observation-based weighting schemes with those based on uniform weighting. For the case of the GPS IIR/-M and III satellites, systematic differences of 10 mm in the PVs and 65 cm in the corresponding PCOs are identified. In addition, new antenna models for the different blocks of BeiDou-3 satellites in medium Earth orbit are derived using different processing schemes. As a drawback of traditional approaches estimating PCOs and PVs consecutively in distinct steps, it is shown that different, albeit self-consistent, PCO/PV pairs may result depending on whether PCOs or PVs are estimated first. This apparent discrepancy can be attributed to potentially inconsistent weighting functions in the individual processing steps. Use of a single-step process is therefore proposed, in which a dedicated constraint for PCO-PV separation is applied in the solution of the normal equations. Finally, the impact of neglecting phase patterns in precise point positioning applications is investigated. In addition to an overall increase of the position scatter, the occurrence of systematic height biases is illustrated. While observation-based weighting in the pattern estimation can help to avoid such biases, the possible benefit depends critically on the specific elevation-dependent weighting applied in the user’s positioning model. As such, the practical advantage of such antenna models would remain limited, and uniform weighting is recommended as a lean and transparent approach for the pattern estimation of satellite antenna models from observations.

Fabrication of Oil-Absorbing Porous Sponges via 3D Electrospinning of Recycled Expanded Polystyrene with Functional Additive

In this study, a three-dimensional (3D) porous sponge capable of oil–water separation was fabricated using recycled expanded polystyrene (EPS) through 3D electrospinning, by adding phosphoric acid to the electrospinning solution. The fabrication process was a rapid and efficient single-step process to produce the 3D sponge. In addition, the additive’s concentration was also optimized for oil absorption. The fabricated EPS sponge was highly effective in oil–water separation due to its excellent hydrophobic and oleophilic properties. This demonstrates its potential as a sustainable and efficient absorbent to address ongoing oil pollution issues. Moreover, the performance of the recycled EPS sponge was found to be comparable to that of sponges made from virgin polystyrene, suggesting the feasibility of using recycled materials for the production of high-value products. This research presents an efficient method for fabricating 3D sponges from recycled materials, contributing to environmental protection and resource recycling.

Flat-Knitted Double-Tube Structure Capacitive Pressure Sensors Integrated into Fingertips of Fully Fashioned Glove Intended for Therapeutic Use

A therapeutic glove, which enables medical non-professionals to perform physiotherapeutic gripping and holding movements on patients, would significantly improve the healthcare situation in physiotherapy. The glove aims to detect the orthogonal pressure load and provide feedback to the user. The use of textile materials for the glove assures comfort and a good fit for the user. This, in turn, implies a textile realization of the sensor system in order to manufacture both the glove and the sensor system in as few process steps as possible, using only one textile manufacturing technique. The flat knitting technology is an obvious choice here. The aim of the study is to develop a textile capacitive pressure sensor that can be integrated into the fingertips of a glove using flat knitting technology and to evaluate its sensor properties with regard to transmission behavior, hysteresis and drift. It was shown that the proposed method of a flat knitting sensor fabrication is suitable for producing both the sensors and the glove in one single process step. In addition, the implementation of an entire glove with integrated pressure sensors, including the necessary electrical connection of the sensor electrodes via knitted conductive paths in three fingers, was successfully demonstrated.

Development of a single-unit SMB process for clean production of 2’-fluoro nucleoside phosphoramidites to be used as the key building blocks of antisense oligonucleotide drugs and mRNA-vaccine capping primers

Due to the recent increase in incurable diseases and the pandemic of COVID-19, there have been global interests in antisense 2’-fluoro modified oligonucleotide drugs and mRNA-vaccine capping primers. To ensure stable and economical production of these oligonucleotides and primers, it is essential to establish a reliable process for securing a large amount of their corresponding monomer (2’-fluoro nucleoside phosphoramidite, abbreviated as “2’F-NP” hereafter) at high purity (> 99%) from the output of the relevant reaction. Currently, this task has been handled using two-stage separation processes (extraction and chromatography in series) based on batch (non-continuous) modes, which, however, had the problems of excessive solvent consumption and low yield. For cleaner production of 2’F-NP, these problems should be resolved. To address such issue, this study aimed to develop a new single-step process that can recover 2’F-NP at once in a continuous mode at high purity. For this purpose, we introduced a simulated-moving-bed (SMB) method into the development phase of the targeted 2’F-NP recovery process, and further established the appropriate SMB configuration and solvent-gradient strategy to continuously remove all the impurities at once. The SMB mode process determined based on such considerations was then optimized using the intrinsic parameters estimated, the regeneration method explored, and the optimization tool prepared. The relevant SMB experiment was carried out, which confirmed that the developed process in this study was quite effective in continuous-mode recovery of 2’F-NP at high purity (99.5%) and high yield (96.5%) without the aid of a further process, thereby enabling a significant reduction in solvent consumption. The results in this paper will thus contribute to the clean and eco-friendly production of 2’F-NP, which will also be of benefit to antisense oligonucleotide drug and mRNA-vaccine capping primer production processes.

High-efficient white blood cell separation from whole blood using cascaded inertial microfluidics

White blood cells (WBCs) are a crucial component of the human immune system. WBCs contain invaluable information about the health status of the human body. Therefore, separating WBCs is indispensable for the diagnosis of many diseases in clinical setting. The low ratio of WBCs to red blood cells in whole blood has made the isolation of WBCs challenging. As the conventional single-stage microfluidic technology cannot provide sufficient separation purity. We used a cascaded inertial microfluidic chip by consecutively connecting two sinusoidal channels to enhance the purity of WBCs after single processing. The improvement was in part due to the diversion of the sample at the end of the first stage separation, resulting in a lower flow rate in the second stage of processing within the cascaded device. We embedded concave micro-obstacles in sinusoidal channels to adjust their effective working flow rate range and enable the proper operation of both channels simultaneously. Using polystyrene beads mixture (5 and 10 μm) with a primary ratio of 1000 to 1, a single processing step through our cascaded chip improved the purity of 10-μm particles with more than three orders of magnitude of enrichment (from 0.08 % to 99.83 %) with a flow rate of 560 μL/min (Re = 77). Using diluted whole blood ( × 1/10), we achieved 307-fold enrichment of WBCs (0.14 %–43.017 %) in a single process which was accompanied with ∼3 orders of magnitude background removal of RBCs (from 4.8 × 108 to 5.7 × 105 counts/mL). This cascaded manner chip has the capacity to achieve high-efficiency separation of blood cells for clinical diagnosis.

Optimizing Carbon Structures in Laser-Induced Graphene Electrodes Using Design of Experiments for Enhanced Electrochemical Sensing Characteristics.

In this study, we explored the morphological and electrochemical properties of carbon-based electrodes derived from laser-induced graphene (LIG) and compared them to commercially available graphene-sheet screen-printed electrodes (GS-SPEs). By optimizing the laser parameters (average laser power, speed, and focus) using a design of experiments response surface (DoE-RS) approach, binder-free LIG electrodes were achieved in a single-step process. Traditional trial-and-error methods can be time-consuming and may not capture the interactions between all variables effectively. To address this, we focused on linear resistance and substrate delamination to streamline the DoE-RS optimization process. Two LIGs, designated LIG A and LIG B, were fabricated using distinct and optimized laser settings, which resulted in a sheet resistance of 25 ± 2 Ω/sq and 21 ± 1 Ω/sq, respectively. These LIGs, characterized by scanning electron microscopy, Raman spectroscopy, and contact angle analysis, exhibited a highly porous morphology with 13% pore coverage and a contact angle <50°, which significantly increased their hydrophilicity when compared to the GS-SPE. For the electrochemical studies, the oxidation of NO2- ion by the graphene-based working electrodes was investigated, as it allowed for the direct comparison of the LIGs to the GS-SPE. These included cyclic voltammetry, electrochemical impedance spectroscopy, and differential pulsed voltammetry studies, which revealed that LIG electrodes displayed a remarkable 500% increase in peak current during NO2- oxidation compared to the GS-SPE. The LIGs also demonstrated improved stability and sensitivity (420 ± 30 and 570 ± 10 nAμM-1 cm-2) compared to the GS-SPE (73 ± 4 nAμM-1 cm-2) in the oxidation of NO2- ions; however, LIG B was more susceptible to ionic interference than LIG A. These findings highlight the value of applying statistical approaches such as DoE-RS to systematically improve the LIG fabrication process, enabling the rapid production of optimized LIGs that outperform conventional carbon-based electrodes.

In Situ Fluorescent Visualization of the Interfacial Layer of Induced Crystallization in Polyvinyl Chloride.

Despite the remarkable progress in the modification and application of polyvinyl chloride (PVC), developing processing aids for the induced crystallization of PVC and characterizing its interfacial layer remain challenges. Herein, we propose a new polymeric nucleating agent, polyamidea12-graft-styrene-maleic anhydride copolymer (PA12-g-SMA), which possesses high compatibility and crystallinity, effectively improving the crystallinity to 15.1%, the impact strength to 61.03 kJ/m2, and the degradation temperature of PVC to 267 °C through a single and straightforward processing step. Additionally, after the introduction of two different fluorescent sensors in PA12-g-SMA and PVC, the interfacial layer of the induced crystallization can be monitored in situ via a confocal laser scanning microscope (CLSM). This study highlights a rare strategy for significantly enhancing the physical properties of rigid PVC through simply adding a polymeric nucleating agent during processing, while also emphasizing the importance of visualizing the interfacial layer to understand various polymer crystallization processes.

Sustainable Removal of Chloroquine from Aqueous Solutions Using Microwave-Activated Cassava Biochar Derived from Agricultural Waste

This study presents a sustainable solution for the removal of the emerging contaminant chloroquine from aqueous solutions, utilizing biochar synthesized from cassava waste through a rapid, single-step microwave activation process. By repurposing cassava waste, a prevalent agricultural by-product, this method aligns with circular economy principles, promoting the sustainable reuse of waste materials. Characterization of the biochar demonstrated a highly porous, crystalline structure optimized for adsorption applications. Adsorption studies demonstrated optimal performance at 45 °C, with a maximum adsorption capacity of 39 mg g−1 in the Langmuir model. Thermodynamic analysis confirmed that the process was spontaneous, endothermic, and consistent with physisorption. Kinetic experiments revealed that 200 rpm agitation provided the most favorable conditions. Notably, the biochar demonstrated substantial reusability, maintaining up to 70% of its adsorption capacity over five desorption cycles. This sustainable adsorbent stands out as a practical, eco-friendly option for removing pharmaceutical contaminants while also corroborating with the beneficial reuse of agricultural by-products.

Single-Step Process for Isolation of Pure Quercetin from Aqueous Extract of Waste Onion Peels

Single-Step Process for Isolation of Pure Quercetin from Aqueous Extract of Waste Onion Peels

Enhancing the performance of unitized regenerative proton exchange membrane fuel cells through microwave-synthesized chitosan based nanocomposites

The membrane electrode assembly (MEA) is a core component of unitized regenerative proton exchange membrane fuel cells (UR-PEMFCs). The studies aimed to improve the cell performance and reduce the cost of the MEAs for the widespread adoption of UR-PEMFCs. The present study focuses on modifications of MEA. For this purpose, an innovative nanocomposite electrocatalyst was developed by using a carbon-based support material containing platinum nanoparticles with a diameter of approximately 20–30 nm via microwave synthesis technique. The electrocatalyst was developed by a single-step process, consist of multi-walled carbon nanotubes (MWCNT), graphitic carbon nitride (g-C3N4), chitosan (Chi), and platinum nanoparticles (MWCNT/g-C3N4/Chi/Pt nanocomposite). With the development of this support material, a relatively economical and effective electrocatalyst was obtained by large surface area and using the platinum on this surface at the nano level. The prepared catalyst was applied to commercially available membrane electrode assemblies with an active area of 100 cm2. Single-cell and triple-stack performance tests were conducted, and an increase of 17.13 % in the electrolyzer mode and 16.98 % in the fuel cell mode was achieved in single-cell performance with this applied electrocatalyst. Furthermore, an enhancement of 16.96 % in the electrolyzer mode and 16.81 % in the fuel cell mode was discerned in the UR-PEMFC stack. Beside the experimental studies, a numerical model of the modified membrane properties has been developed and validated through experimental data.

Single-step process for creating nitrogen and oxygen-enriched carbon using organic polymers for supercapacitor applications

Single-step process for creating nitrogen and oxygen-enriched carbon using organic polymers for supercapacitor applications

Design of Lignin-Based TiO2 Composite for Enhanced Photocatalytic Activity and its Applications

Lignin with TiO2 necessitates careful consideration of the organic-to-inorganic ratio in order to achieve the desired functional characteristics. This study synthesized a Lignin-TiO2 hybrid material through a single-step process utilizing the Taguchi design, which significantly enhanced photocatalytic efficiency under visible light. The Lignin-TiO2 composite demonstrated improved dispersion, negative surface charge, lower bandgap energy, and reduced charge carrier recombination compared to the controls, resulting in a 2.2-fold increase in photocatalytic efficiency for the removal of methylene blue (MB) dye relative to pure TiO2. Additionally, lignin was found to be an effective inhibitor of photo-corrosion, significantly enhancing the stability of Lignin-TiO2 during 690minutes of irradiation. The role of OH radicals in MB photodegradation was confirmed by the degradation of coumarin to 7-hydroxycoumarin (7-OHC). Moreover, Lignin-TiO2 displayed antimicrobial activity against S. aureus and showed potential as a filler in polyvinyl alcohol (PVA), extending the shelf life of cherry tomatoes by up to 7 days. This study presents a promising approach to enhancing visible light-active photocatalysts using renewable materials while highlighting the practical applications of the composite in environmental remediation and the preservation of agricultural products.

FastProtein-an automated software for in silico proteomic analysis.

Although various tools provide proteomic information, each tool has limitations related to execution platforms, libraries, versions, and data output format. Integrating data generated from different software is a laborious process that can prolong analysis time. Here, we present FastProtein, a protein analysis pipeline that is user-friendly, easily installable, and outputs important information about subcellular location, transmembrane domains, signal peptide, molecular weight, isoelectric point, hydropathy, aromaticity, gene ontology, endoplasmic reticulum retention domains, and N-glycosylation domains. It also helps determine the presence of glycosylphosphatidylinositol and obtain functional information from InterProScan, PANTHER, Pfam, and alignment-based annotation searches. FastProtein provides the scientific community with an easy-to-use computational tool for proteomic data analysis. It is applicable to both small datasets and proteome-wide studies. It can be used through the command line interface mode or a web interface installed on a local server. FastProtein significantly enhances proteomics analysis workflows by producing multiple results in a single-step process, thereby streamlining and accelerating the overall analysis. The software is open-source and freely available. Installation and execution instructions, as well as the source code and test files generated for tool validation, are available at https://github.com/bioinformatics-ufsc/FastProtein.

Extrusion-Based Three-Dimensional Printing of Metronidazole Immediate Release Tablets: Impact of Processing Parameters and in Vitro Evaluation

PurposeThe current study assessed the potential of a pneumatic 3D printer in developing a taste-masked tablet in a single step. Metronidazole (MTZ) was chosen as the model drug, and Eudragit® E PO was used as a taste-masking polymer to produce taste-masked tablets.MethodsThe study focused on optimizing processing parameters, such as the nozzle's printing speed, the printhead's heating temperature, and the pressure. Oval-shaped tablets were printed with a rectilinear printing pattern of 30% and 100% infill and evaluated for in vitro drug release and taste masking. The 3D-printed tablets are also characterized using Differential Scanning Calorimetry (DSC), Fourier-transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM).ResultsThe infill density impacts the drug release profile of the tablets. F9, F10, and F11 displayed desired printability among the formulations, with F9 and F10 exhibiting over 85% drug release within 60 min in the in vitro dissolution study. The F9 formulation, with 30% infill, effectively masked the bitter taste of MTZ in the in vitro dissolution study carried out in a pH 6.8 artificial salivary medium. The observed release was below the tasting threshold concentration of the model drug.ConclusionIn summary, 3-dimensional extrusion-based printing combines the effects of hot-melt extrusion and fused deposition modeling techniques in a single-step process, demonstrating potential as an alternative to the fused-deposition model 3D printing technique and warranting further exploration.Graphical

Efficient production of activated carbons from PET for organic supercapacitor applications: A single-step approach

This study introduces a novel approach for synthesizing supercapacitor electrode materials using polyethylene terephthalate (PET), a plastic facing recycling challenges. Unlike conventional methods that involve separate carbonization and activation steps, we employ a single-step method. In this process, activated carbons are synthesized using potassium hydroxide (KOH) as an activating agent. Remarkably, the yield via the single-step method (S_PACXs) exceeds that of the two-step method (T_PACYs) by at least 1.7 times. During the single-step process, KOH forms a layer on the PET surface before activation, which leads to increased yields, higher specific surface areas, and more developed mesopores. Furthermore, S_PACXs exhibit superior specific surface areas compared to commercial activated carbon. These enhanced properties significantly improve electrochemical performance, with S_PACXs demonstrating superior performance compared to T_PACYs. Ultimately, this study validates the efficiency of the single-step method in producing high-quality activated carbon from PET, saving time and energy, and outperforming the two-step method.

Investigation on precipitate behavior and mechanical properties of Al-Zn-Mg-Cu alloys with various Zn/Mg ratios

The effect of Zn/Mg ratio on the microstructure and properties of Al-Zn-Mg-Cu alloys under single-step and double-step aging processes were investigated by using scanning electron microscopy (SEM), transmission electron microscopy (TEM), electron backscatter diffraction (EBSD), as well as hardness, room temperature tensile, and fracture toughness testing. During single-step aging, as the Zn/Mg ratio increases, the aging response of the alloys accelerates. However, the peak hardness and strength gradually decreased while the average size of precipitates increased. During double-step aging, as the secondary aging time increases, the strength and hardness of the alloys gradually decrease. Additionally, the higher the Zn/Mg ratio, the faster the strength and hardness decline. Under the T74 temper, the alloy with higher Zn/Mg ratios exhibits lower hardness and strength but better fracture toughness and larger precipitate sizes. A simple method was proposed to statistically determine the critical diameter size of GPII zone and η′ phase, which was approximately 4.47 nm. A strength model was developed to predict the yield strength of alloys, considering factors such as solid solution strengthening, grain boundary strengthening, and precipitation strengthening. The model demonstrates good predictive accuracy for both peak-aged and T74 temper alloys, providing valuable guidance for alloy design and microstructure regulation.

Microfluidic capillary platform with hydrophilic PDMS for point-of-care immunoassays

Point-of-care (PoC) devices offer the possibility of fast and easy to use testing platforms. Self-driven capillary devices are particularly interesting because they can function without external pumps. Microfluidic devices often rely on polydimethylsiloxane (PDMS) prototyping; however, for self-driven microfluidics, its hydrophobic nature makes its use challenging. Bulk modification of PDMS through copolymers with hydrophilic moieties could be a practical solution because the copolymer is added prior to PDMS baking in a single-step process. In this work, a PDMS bulk modification with dimethylsiloxane-(60–70 % ethylene oxide) block copolymer was used to render PDMS hydrophilic. The addition of 1 % (w/w) copolymer resulted in a hydrophilic PDMS which was then used to fabricate the capillary devices. The microfluidic design included a serpentine channel with reagent entry points connected to a capillary pump and was closed by a membrane layer with conical shaped reservoirs. The reservoirs were designed to allow the sequential entry of the solutions and the device sealing and robustness were improved through PMMA frames with integrated magnets. A proof-of-concept was performed with food colouring demonstrating the capability of the device to flow the solutions sequentially. Finally, an immunoassay to detect the presence of Infliximab, a therapeutic antibody for chronic inflammatory diseases, was used to demonstrate the functionality of the capillary device. The versatility of the device was also shown by performing a fluorescent indirect immunoassay and a colorimetric indirect-ELISA for the detection of Infliximab with both approaches detecting Infliximab in the clinical range of interest (between 3 and 7 μg/mL).

Scalable fabrication approach and fill factor optimization for single pixel microlens arrays

Microlenses are a suitable approach to improve the performance of optical systems. An important optical efficiency determining parameter is the fill factor, which describes the relation of the lens area to the total optical active area. In this work, an optimization of the fill factor by optimizing the fabrication process steps is presented. The approach here is the use of i-line waferstepper lithography in combination with thermal reflow of photoresist and subsequent 1:1 pattern transfer in the lens material by reactive ion etching. For this method, the fill factor is determined by the minimum lens gap and, thus, the optical efficiency is strongly limited by the resolution limit of the i-line waferstepper lithography (350 nm). The goal of this investigation is to achieve the lowest possible lens gap even below the stepper-based resolution limit by optimizing each single process step without developing a new approach. The final result of the optimization was a fill factor improvement of 15%.