Single Organism Research Articles

ПРЕИМУЩЕСТВА ИСПОЛЬЗОВАНИЯ ПАРТЕНОГЕНЕТИЧЕСКИХ КЛОНОВ ТУТОВОГО ШЕЛКОПРЯДА (BOMBYX MORI L.) В ГИБРИДИЗАЦИИ

The fierce competition in the global silk market encourages the development and application of new biotechnologies in sericulture, such as cloning. The term “clone” in Greek means “branch”. A clone is an offspring of a single organism propagated without fertilization. All cloned individuals are genetically identical and are copies of each other. Clones are obtained in different ways. In case of the silkworm, this is a parthenogenetic development, which is an unnatural way of reproduction for the silkworm. Thermoactivation of an unfertilized greene at t0=46 0C during 18, leads to inhibition of the reduction division of meiosis in the silkworm germ cells. As a result, the eggs remain with a diploid set of chromosomes and develop as zygotes. Since the female cells in the sex chromosomes of the silkworm are heterogametic, only female parthenogenetic clones develop from thermally activated eggs. This feature makes silkworm clones extremely attractive for creating 100% pure hybrids. As the sericulture globally is based on the production of hybrids of the first generation only, in order to use maximum heterosis, the accuracy of preparation of hybrids is becoming particularly important.

PERAN TOKOH AGAMA DALAM MEMBENTUK PERILAKU KEAGAMAAN PADA MASYARAKAT YANG GEMAR TERHADAP ORGEN TUNGGAL DI KELURAHAN 15 ULU KECAMATAN JAKABARING PALEMBANG

The purpose of this study is to explain how the contribution of religious figures in shaping religious behavior in people who are fond of single organism and what are the supporting factors and constraints of religious figures in shaping religious behavior in people who like single organism in Kelurahan 15 Ulu, Jakabaring District, Palembang. The data collection method uses the interview method, documentation observation, the analysis used is a qualitative descriptive approach. The research argues that the role of religious figures in Kelurahan 15 Ulu, Jakabaring Palembang Subdistrict has been good in its implementation, but ineffective in its approach to the community, because religious figures do not really provide counseling or warning advice to people who like single organizations in broadcasting religious knowledge. Sometimes only during the lecture at the wedding reception there is a little question of public discipline in the entertainment of the single organization itself. And it just so happens that most people in Kelurahan 15 Ulu, Jakabaring Subdistrict, Palembang, are less aware of the knowledge of Religion and religious behavior. So that awareness of the knowledge of Religion in Kelurahan 15 Ulu Sub-district of Jakabaring Palembang is lacking in terms of entertainment for the single organization itself.

Multiple mitochondrial haplotypes within individual specimens may interfere with species identification and biodiversity estimation by DNA barcoding and metabarcoding in fig wasps

AbstractAccurate assessment of biodiversity is important for scientists, conservationists and policy makers. With the development of DNA barcoding and metabarcoding, many cryptic species have been identified based on the sequence diversity of a ‘barcode’ region in the mitochondrial genome. These molecular identifications have increased the estimated number of species on Earth ten‐fold. Mitochondrial DNA heteroplasmy, in which multiple mitochondrial haplotypes coexist in a single organism, can interfere with the accuracy of these estimates. However, this phenomenon is still infrequently documented. The main reason is that it is difficult to distinguish heteroplasmic sequences from nuclear mitochondrial segments (Numts). We investigated the prevalence of multiple haplotypes in DNA barcoding sequences in fig wasps (Insect: Hymenoptera) by performing high‐throughput sequencing on individual specimens. DNA metabarcoding was artificially simulated by combining all the haplotype sequences. Using conventional DNA barcoding by Sanger sequencing, the 28 morphological species we studied were divided into 38 taxa, suggesting the existence of ten cryptic species. However, the haplotypes originating from a single specimen clustered into different cryptic species clades, which conflicted with the conventional barcoding results. Furthermore, when we artificially simulated DNA metabarcoding by mixing all haplotype sequences, the number of molecular operational taxonomic units (or putative species) was 3.2× the number of morphological species. These results indicate that the prevalence of multiple haplotypes within individual specimens may have an important impact on DNA barcode‐based species identification and biodiversity assessment more broadly.

Ecophysiological differentiation between life stages in filmy ferns (Hymenophyllaceae).

Desiccation tolerance was a key trait that allowed plants to colonize land. However, little is known about the transition from desiccation tolerant non-vascular plants to desiccation sensitive vascular ones. Filmy ferns (Hymenophyllaceae) represent a useful system to investigate how water-stress strategies differ between non-vascular and vascular stages within a single organism because they have vascularized sporophytes and nonvascular gametophytes that are each capable of varying degrees of desiccation tolerance. To explore this, we surveyed sporophytes and gametophytes of 19 species (22 taxa including varieties) of filmy ferns on Moorea (French Polynesia) and used chlorophyll fluorescence to measure desiccation tolerance and light responses. We conducted phylogenetically informed analyses to identify differences in physiology between life stages and growth habits. Gametophytes had similar or less desiccation tolerance (ability to recover from 2 days desiccation at - 86MPa) and lower photosynthetic optima (maximum electron transport rate of photosystem II and light level at 95% of that rate) than sporophytes. Epiphytes were more tolerant of desiccation than terrestrial species in both life stages. Despite their lack of greater physiological tolerances, gametophytes of several species occurred over a wider elevational range than conspecific sporophytes. Our results demonstrate that filmy fern gametophytes and sporophytes differ in their physiology and niche requirements, and point to the importance of microhabitat in shaping the evolution of water-use strategies in vascular plants.

Light Harvesting in Fluctuating Environments: Evolution and Function of Antenna Proteins across Photosynthetic Lineage.

Photosynthesis is the major natural process that can harvest and harness solar energy into chemical energy. Photosynthesis is performed by a vast number of organisms from single cellular bacteria to higher plants and to make the process efficient, all photosynthetic organisms possess a special type of pigment protein complex(es) that is (are) capable of trapping light energy, known as photosynthetic light-harvesting antennae. From an evolutionary point of view, simpler (unicellular) organisms typically have a simple antenna, whereas higher plants possess complex antenna systems. The higher complexity of the antenna systems provides efficient fine tuning of photosynthesis. This relationship between the complexity of the antenna and the increasing complexity of the organism is mainly related to the remarkable acclimation capability of complex organisms under fluctuating environmental conditions. These antenna complexes not only harvest light, but also provide photoprotection under fluctuating light conditions. In this review, the evolution, structure, and function of different antenna complexes, from single cellular organisms to higher plants, are discussed in the context of the ability to acclimate and adapt to cope under fluctuating environmental conditions.

Mantis: flexible and consensus-driven genome annotation.

BackgroundThe rapid development of the (meta-)omics fields has produced an unprecedented amount of high-resolution and high-fidelity data. Through the use of these datasets we can infer the role of previously functionally unannotated proteins from single organisms and consortia. In this context, protein function annotation can be described as the identification of regions of interest (i.e., domains) in protein sequences and the assignment of biological functions. Despite the existence of numerous tools, challenges remain in terms of speed, flexibility, and reproducibility. In the big data era, it is also increasingly important to cease limiting our findings to a single reference, coalescing knowledge from different data sources, and thus overcoming some limitations in overly relying on computationally generated data from single sources.ResultsWe implemented a protein annotation tool, Mantis, which uses database identifiers intersection and text mining to integrate knowledge from multiple reference data sources into a single consensus-driven output. Mantis is flexible, allowing for the customization of reference data and execution parameters, and is reproducible across different research goals and user environments. We implemented a depth-first search algorithm for domain-specific annotation, which significantly improved annotation performance compared to sequence-wide annotation. The parallelized implementation of Mantis results in short runtimes while also outputting high coverage and high-quality protein function annotations.ConclusionsMantis is a protein function annotation tool that produces high-quality consensus-driven protein annotations. It is easy to set up, customize, and use, scaling from single genomes to large metagenomes. Mantis is available under the MIT license at https://github.com/PedroMTQ/mantis.

SUSTAINABLE DEVELOPMENT OF INTERACTION OF INDUSTRIAL AND TRADE POLICY IN SOCIALLY SIGNIFICANT INDUSTRIES

The development of socially oriented industries is a fundamental component of the socio-economic development of the country. The co-dependence of industries and the state of industry in it gives the research topic relevance, since consumer satisfaction can be achieved both with the help of their own industrial development and with cooperation with foreign industrial companies. The problem of the issue is precisely that most branches of the domestic processing industry, including socially significant ones, are import-dependent, and the level at which the interaction of industrial and trade policy (hereinafter referred to as the IITP) in them determines the future of their further development. The purpose of the study is to determine the essence of the IITP, the sustainability (and) development in socially oriented industries by considering the development both within the interaction itself (setting priorities and distributing roles among the main participants in the interaction of industrial and trade policy), and within its phased implementation as a single organism. The achievement of the author's goal was facilitated by strategic, system and process approaches using general scientific research methods: comparative comparison, abstraction, logic, analysis, synthesis, etc. As a result of the conducted research, the scheme of interaction between the spheres of material and non-material production is presented; the definitions of the IITP are formulated; a cycle-model of the development of the IITP in socially oriented sectors of the national economy is developed; a comprehensive analysis of the set of certain indicators is recommended to assess the sustainability of the IITP development; the definition of the sustainable IITP development from the position of system formation and economic activity is presented.

Influence of full-scale climatic tests on the properties of aluminum alloys in zones of river and marine water mixture

Within nine months, comprehensive studies were conducted on the effect of full-scale climatic tests on the properties of samples of aluminum alloy AMg6 when fully submerged in the estuary region of the Don River (the basin of the Sea of Azov) at two stations. The features of the formation of micro- and macro-fouling communities on experimental plates at one, three, six and nine months after the start of the experiment are shown. It is shown that microfouling communities on the experimental plates begin to form within a month of exposure. Diatoms were the background-forming species during the entire practical period of the study. Single macrozoobenthos organisms begin to appear on the samples after three months and only at second station. After six months, macro-fouling organisms are fixed on plates at both stations. As the exposure period increases, the number and biomass of macro-foulers increases. The corrosion resistance assessment of the samples revealed the absence of intercrystalline corrosion during the entire test period in water, at both stations, as well as pitting corrosion during the first six months of exposure.

Eco-Evolutionary Perspectives on Mixoplankton

Mixotrophy, i.e., the capability of both phototrophy and phagotrophy within a single organism, is a prominent trophic mode in aquatic ecosystems. Mixotrophic strategies can be highly advantageous when feeding or photosynthesis alone does not sustain metabolic needs. In the current review, we discuss the functional types of mixotrophic marine protists (herein mixoplankton) within the context of evolution. Permanent plastids have been established in large due to gene transfer from prey and/or endosymbionts to the host cell. In some kleptoplastidic mixoplankton, prior gene transfers and active transcription of plastid related genes in the host can help maintain and extend retention of the current kleptoplast. In addition to kleptoplasts, the prey nucleus is also sometimes retained and actively transcribed to help maintain and even replicate the kleptoplasts. Endosymbiotic relations vary considerably in the extent to which hosts affect symbionts. For example, some endosymbionts are heavily modified to increase photosynthetic efficiency, or are controlled in their cell division. It can be proposed that many kleptoplasts and endosymbionts are in fact en route to becoming permanent plastids. Conditions such as increased temperature and limiting nutrients seem to favor phagotrophy in mixoplankton. However, responses of mixoplankton to changing environmental conditions like light irradiance, temperature, nutrient, and prey availability are variable and species-specific. Studying mixotrophs with temporary plastids could elucidate past and future evolutionary mechanisms and dynamics of processes such as phagotrophy and the establishment of (secondary) permanent plastids.

Host exposure history and priority effects impact the development and reproduction of a dominant parasite

Within a single organism, numerous parasites often compete for space and resources. This competition, together with a parasite’s ability to locate and successfully establish in a host, can contribute to the distribution and prevalence of parasites. Coinfection with trematodes in snail intermediate hosts is rarely observed in nature, partly due to varying competitive abilities among parasite taxa. Using a freshwater snail host (Biomphalaria glabrata), we studied the ability of a competitively dominant trematode, Echinostoma caproni, to establish and reproduce in a host previously infected with a less competitive trematode species, Schistosoma mansoni. Snails were exposed to S. mansoni and co-exposed to E. caproni either simultaneously or 1 week, 4 weeks, or 6 weeks post S. mansoni exposure. Over the course of infection, we monitored the competitive success of the dominant trematode through infection prevalence, parasite development time, and parasite reproductive output. Infection prevalence of E. caproni did not differ among co-exposed groups or between co-exposed and single exposed groups. However, E. caproni infections in co-exposed hosts took longer to reach maturity when the timing between co-exposures increased. All co-exposed groups had higher E. caproni reproductive output than single exposures. We show that although timing of co-exposure affects the development time of parasite transmission stages, it is not important for successful establishment. Additionally, co-exposure, but not priority effects, increases the reproductive output of the dominant parasite.

Treatment of positive urine cultures in the neonatal intensive care unit: a guideline to reduce antibiotic utilization

The pediatric definition of bacterial urinary tract infection (UTI) is >50,000 colony forming units (CFU) of a single organism on catheterized culture or 10,000-50,000 CFU with pyuria on urinalysis. The diagnosis of UTI in our NICU is clinician-dependent and not based on the accepted pediatric definition. A retrospective review of positive urine cultures between 2015 and 2017 was performed. A treatment guideline for positive urine cultures was adopted and PDSA methodology utilized for incremental improvements. For 909 pre-intervention neonates, 26 of 38 positive urine cultures were treated for UTI but only 23% (6/26) met the pediatric definition. For 644 post-guideline neonates, only 7 of 25 positive urine cultures were treated and 86% met guideline criteria with no increase in urosepsis. A guideline to treat positive urine cultures resulted in a decreased rate of UTI diagnosis and thus prevented unnecessary antibiotic exposure.

Bacterial Cell, Classification and Required Essential Contents for Growth

The bacterial cell is generally single cellular micro organism that spread easily around us and infect host cell. In this review we discuss about the types and nutrient requirements for growth and spreading of bacteria. The bacteria are various types like as Phototrophs, Autotrophs, Psychrophiles, Mycoplasma etc.

Design processes and multi-regulation of biomimetic building skins: A comparative analysis

Biomimetics is an opportunity for the development of energy efficient building systems. Several biomimetic building skins (Bio-BS) have been built over the past decade, however few addressed multi-regulation although the biological systems they are inspired by have multi-functional properties. Recent studies have suggested that despite numerous tools and methods described in the literature for the development of biomimetic systems, their use for designing Bio-BS is scarce. To assess the main challenges of biomimetic design processes and their influence on the final design, this paper presents a comparative analysis of several existing Bio-BS. The analyses were carried out with univariable and multivariate descriptive tools in order to highlight the main trends, similarities and differences between the projects. The authors evaluated the design process of thirty existing Bio-BS, including a focus on the steps related to the understanding of the biological models. Data was collected throughout interviews. The univariate analysis revealed that very little Bio-BS followed a biomimetic design framework (5%). None of the Bio-BS was as multi-functional as their biological model(s) of inspiration. A further conclusion drawn that Bio-BS are mostly inspired by single biological organisms (82%), which mostly belong to the kingdom of animals (53%) and plants (37%). The multivariate analysis outlined that the Bio-BS were distributed into two main groups: (1) academic projects which present a strong correlation with the inputs in biology in their design processes and resulted in radical innovation; (2) public building projects which used conventional design and construction methods for incremental innovation by improving existing building systems. These projects did not involve biologists neither a thorough understanding of biological models during their design process. Since some biomimetic tools are available and Bio-BS have shown limitations in terms of multifunctionality, there is a need to promote the use of multidisciplinary tools in the design process of Bio-BS, and address the needs of the designers to enhance the application of multi-regulation capabilities for improved performances.

3D mechanical characterization of single cells and small organisms using acoustic manipulation and force microscopy

Quantitative micromechanical characterization of single cells and multicellular tissues or organisms is of fundamental importance to the study of cellular growth, morphogenesis, and cell-cell interactions. However, due to limited manipulation capabilities at the microscale, systems used for mechanical characterizations struggle to provide complete three-dimensional coverage of individual specimens. Here, we combine an acoustically driven manipulation device with a micro-force sensor to freely rotate biological samples and quantify mechanical properties at multiple regions of interest within a specimen. The versatility of this tool is demonstrated through the analysis of single Lilium longiflorum pollen grains, in combination with numerical simulations, and individual Caenorhabditis elegans nematodes. It reveals local variations in apparent stiffness for single specimens, providing previously inaccessible information and datasets on mechanical properties that serve as the basis for biophysical modelling and allow deeper insights into the biomechanics of these living systems.

A New Approach to Produce Succinic Acid Through a Co-Culture System.

Microorganisms can produce a wide range of bio-based chemicals that can be used in various industrial applications as molecules of interest. In the present work, an analysis of the power production by pure culture, co-culture, and sequential culture was performed. In this study, both the mono-culture and the co-culture strategies of Actinobacillus succinogenes with Saccharomyces cerevisiae as carbon sources to produce succinic acid using glucose and fructose were examined. The cultures were performed in batch mode and a great attention was paid to the co-culture system to improve the biosynthetic pathway between A. succinogenes and S. cerevisiae by combining these two strains in a single fermentation process. Under microaerobic and anaerobic conditions, the process was characterized in terms of sugars concentration, cell density, metabolites, yield (mol-C products/ mol-C sugars), the temperature conditions for productivity, and pH. The results showed that the process could consume glucose and fructose and could adapt to different concentrations of the two sugars more quickly than by a single organism and the best results were obtained in a sequential co-culture recording 0.27 mol L-1 of succinic acid concentration and a volumetric productivity of 0.3 g L-1 h-1. Under the investigated operating conditions, the combination of these two strains in a single reactor produced a significant amount of succinic acid (0.70 mol-C SA/mol-C substrates). A simultaneous and sequential co-culture strategy can be a powerful new approach in the field of bio-based chemical production.

ASSOCIATION OF ASYMPTOMATIC BACTERIURIA AND PRE ECLAMPSIA IN EL SHATBY UNIVERSITY HOSPITAL

Background: Asymptomatic bacteriuria is defined as thepresence of significant bacteriuria>105 colony formingunits (cfu)/mL without the symptoms of an acute urinarytract infection. Pre-eclampsia is defined by blood pressureof greater than 140/90 mmHg after the 20th week of pregnancy and proteinuria of equal to or higher than 300 mg per24 hours.Objective: Detecting the incidence of asymptomatic bacteriuria among study group at El shatby antenatal care clinic and investigate the association between development ofPE and presence of ASB.Subjective: The study included 80 pregnant women.Quantitative culture remains the gold standard for diagnosis of urinary tract infection in pregnancy,Microscopic examination of a wet film of uncentrifuged urinewas carried out to detect the presence of pyuria, whichis considered when pus cells are >5 pus cells / HPF.Amount of urine (1 μl) is inoculated on MacConkeyagar,blood agar and sabouraud dextrose agar plates. Theplates are incubated for 24-48 hours at 37°C. After incubation, the colony count of a pure single organism is performed a significant bacteriuria is considered when the colony count is >105 (cfu/ml).Results: The net result of our study showed that the patients were screened for asymptomatic bacteriuria and theincidence was 30%. Asymptomatic bacteruria was morecommon in preeclamptic group (37%) than control group (22%) but with no significant difference.Conclusion: Asymptomatic bacteriuria was a commonfinding among pregnant women in our study ( 30% ofwhole cases).

Человек и степь в раннем железном веке. Итоги междисциплинарных исследований

Introduction. Recent archaeological studies deal with the integration of natural science disciplines. Such scientific interaction includes the reconstruction of climatic changes, human adaptation to the changing conditions of nature, study of sociocultural specifics in nomadic groups as well as rising archaeological cultures, with emphasis on the interconnection between the fluctuations of steppe environmental conditions and steppe populations. Analysis. The article presents the results of the carried out interdisciplinary analysis of classic and modern archaeological studies and natural science disciplines. This allows evaluating the specifics of different factors (paleoclimatic, sociocultural, etc.) influencing the “steppe-human” system in a new way. Such factors as registered climatic changes, spreading areas of archaeological cultures, mortuary funeral rites, results of paleoanthropological examination, written records and ethnographic data provide evidence to reconstructing different time-span events of early nomads’ history in one context. The reconstruction of historical reality shows quite strong correlation between the environment and specific features in the development of ancient steppe societies. Human has high adaptive abilities to changing factors. However, the steppe population mode of life is extremely conservative and it has practically never changed during the Sarmatian or Sauromatian history. Climate fluctuations over the steppe area influenced the demographic and social structure of nomadic society. During auspicious periods, nomadic communities became populous and active politically and military. If negative factors dominated, the population tended to decrease and the social structure tended to simplify. Critical indicators of aridization and humidization in Eurasian steppes are followed with the population outflow, which is evidenced by small amount of archaeological sites or even by vanishing of cultures. Results. Thus, the authors conclude that when studying archaeological sites of the Early Iron Age nomadic cultures, it is necessary to consider the steppe and human as a single organism responsive to changing and a strong impact of environmental and socio-cultural factors.

Synergistic interactions of sperm impairing bacteria: Impact on pregnancy outcome in mouse model

Earlier in our laboratory, the role of various individual sperm impairing microorganisms on sperm parameters and female infertility has been elucidated at higher doses. As, multiple bacterial species tend to exert more pathogenic effect in comparison to single organism hence, present study was carried out to evaluate that if consortia of these sperm impairing organisms can lead to infertility in female mice at sub fertility dose. For this, impact of individual bacterial strains of Escherichia coli, Pseudomonas aeruginosa, Pseudomonas aeruginosa, Klebsiella pneumoniae and consortia of Escherichia coli and Pseudomonas aeruginosa, Escherichia coli and Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae was examined on the motility, viability of mouse spermatozoa and fertility outcome. The results showed that the individual bacterial strains of E. coli, S. marcescens and K. pneumoniae could led to immobilization of spermatozoa by agglutination and P. aeruginosa led to immobilization of spermatozoa without agglutination. Also, all of them led to 100% sperm death in 45 min of incubation. In case of consortia of bacterial strains, the results showed sperm agglutination in all the cases and they were able to induce 100% sperm death at 30 min of incubation time. Further, in vivo studies were carried out to evaluate the impact of individual bacterial strains and consortia of bacterial strains on the fertility outcome in female Balb/c mice. For this, female mice were administered intravaginally with 101 cfu/20µl of individual bacterial strains or consortia of strains for 10 consecutive days or PBS. The results showed that both individual bacterial strains and consortia of bacterial strains were able to efficiently colonize the mouse vagina. Further, control group receiving phosphate buffer saline and groups receiving individual bacterial strains showed all the pregnancy related changes viz. abdominal distension, string of pearls on palpation as well as delivery of pups on completion of gestation period and delivery of pups. The histological examination of reproductive organs viz. uterus and ovary, of the female mice receiving PBS or individual bacterial strains showed the formation of corpus luteum in the ovary and the formation of decidua’s in the uterus, indicative of pregnancy. However, mice receiving consortia of bacterial strains did not show any pregnancy related changes throughout the experiment. Thus, these results indicate that the presence of consortia of sperm impairing microorganisms in vaginal milieu is efficient in provoking infertility even at subfertility doses.

Profile of aerobic bacteria and their antibiotic sensitivity in chronic suppurative otitis media in Al-Ramadi Teaching Hospital, Ramadi City, Iraq.

Chronic suppurative otitis media (CSOM) is a common otological problem in daily clinical practice. It is crucial to know the bacterial pathogens and their antimicrobial susceptibilities in patients with CSOM to achieve a good clinical outcome. To identify the aerobic bacterial pathogens and their antibiotic sensitivities in subjects with CSOM in Al-Ramadi Teaching Hospital. A cross-sectional, descriptive study included 102 subjects with a clinical diagnosis of CSOM (aural discharge >2 weeks, eardrum perforation, and conductive deafness). Purulent discharge was obtained from the middle ear with sterile swabs and cultured for bacterial microorganisms. The sensitivity of the isolated agents to antimicrobials was evaluated by a culture and sensitivity method. SPSS version 22 was used for statistical analysis of the data. Differences were considered statistically significant at p<0.05. Out of 3634 outpatient subjects, 102 (2.8%) presented with active CSOM. The age range of the patients was 1-70 years (mean 28.90±19.8). There were 58 females (56.9%). Out of 117 ear swab specimens, 107 (91.5%) yielded positive cultures. The majority (101, 94.4%) of the specimens yielded a single organism. There was a high statistically significant difference (p<0.001) between gram-positive (n=77, 68.1%) and gram-negative pathogens (n=36, 31.9%). Pseudomonas aeruginosa in 65 (57.5%) cases and Staphylococcus aureus in 19 (16.8%) cases were the two most commonly isolated organisms. The drugs imipenem (93.8%), amikacin (86.1%), azteronam (83.1%), and ciprofloxacin (81.5%) were effective against P. aeruginosa (p<0.001). Amikacin (100%), imipenem (94.7%), ciprofloxacin (68.4%), and gentamicin (63.1%) were the most effective antibiotics against Staph. aureus (p<0.001). The prevalence rate of active CSOM was 2.8%. Ciprofloxacin showed high effectiveness against the two most common isolated pathogens (P. aeruginosa and Staph. aureus); therefore, it could be used as empirical therapy for active CSOM cases.

Цифрова бізнес-модель промислового підприємства

The formation of a single digital space at the enterprise greatly simplifies its management. Digitalization significantly improves productivity and has already become a top priority for business leaders and organizations around the world. Intelligent production systems fundamentally change the existing image of the industry, there is a qualitative transition to a new industrial order, in which production is managed as a single organism, in which all technological and organizational elements are interconnected.