Single Motor Units Research Articles

Microendoscopy detects altered muscular contractile dynamics in a mouse model of amyotrophic lateral sclerosis

Amyotrophic lateral sclerosis (ALS) is a fatal disease involving motor neuron degeneration. Effective diagnosis of ALS and quantitative monitoring of its progression are crucial to the success of clinical trials. Second harmonic generation (SHG) microendoscopy is an emerging technology for imaging single motor unit contractions. To assess the potential value of microendoscopy for diagnosing and tracking ALS, we monitored motor unit dynamics in a B6.SOD1G93A mouse model of ALS for several weeks. Prior to overt symptoms, muscle twitch rise and relaxation time constants both increased, consistent with a loss of fast-fatigable motor units. These effects became more pronounced with disease progression, consistent with the death of fast fatigue-resistant motor units and superior survival of slow motor units. From these measurements we constructed a physiological metric that reflects the changing distributions of measured motor unit time constants and effectively diagnoses mice before symptomatic onset and tracks disease state. These results indicate that SHG microendoscopy provides a means for developing a quantitative, physiologic characterization of ALS progression.

Genioglossus motor unit activity in supine and upright postures in obstructive sleep apnea.

This study investigated whether a change in posture affected the activity of the upper-airway dilator muscle genioglossus in participants with and without obstructive sleep apnea (OSA). During wakefulness, a monopolar needle electrode was used to record single motor unit activity in genioglossus in supine and upright positions to alter the gravitational load that causes narrowing of the upper airway. Activity from 472 motor units was recorded during quiet breathing in 17 males, nine of whom had OSA. The mean number of motor units for each participant was 11.8 (SD 3.4) in the upright and 16.0 (SD 4.2) in the supine posture. For respiratory-modulated motor units, there were no significant differences in discharge frequencies between healthy controls and participants with OSA. Within each breath, genioglossus activity increased through the recruitment of phasic motor units and an increase in firing rate, with an overall increase of ~6 Hz (50%) across both postures and participant groups. However, the supine posture did not lead to compensatory increases in the peak discharge frequencies of inspiratory and expiratory motor units, despite the increase in gravitational load on the upper airway. Posture also had no significant effect on the discharge frequency of motor units that showed no respiratory modulation during quiet breathing. We postulate that, in wakefulness, any increase in genioglossus activity to compensate for the gravitational effects on the upper airway is achieved primarily through the recruitment of additional motor units in both healthy controls and participants with OSA.

Regional activation in the human longissimus thoracis pars lumborum muscle.

•Longissimus activity in the lumbar region was measured using indwelling electromyography to characterize the territory of its motor units. •The distribution of motor units in the longissimus pars lumborum muscle was mainly grouped into two distinct regions. •Regional activation of the longissimus pars lumborum was also observed during functional tasks involving trunk movements. •The regional activation of the longissimus pars lumborum muscle may play a role in segmental stabilization of the lumbar spine. The longissimus pars lumborum contributes to lumbar postural control and movement. While animal studies suggest a segmental control of this muscle, the territory of motor units constituting the human longissimus pars lumborum remains unknown. The aims of this study were to identify the localization of motor unit territories in the longissimus and assess the activation of this muscle during functional tasks. Eight healthy participants were recruited. During isometric back extension contractions, single motor-unit (at L1, L2, L3 and L4) and multi-unit indwelling recordings (at L1, L1-L2, L2, L2-L3, L3, L3-L4 and L4) were used to estimate motor unit territories in the longissimus pars lumborum based on the motor-unit spike-triggered averages from fine-wire electrodes. A series of functional tasks involving trunk and arm movements were also performed. A total of 73 distinct motor units were identified along the length of the longissimus: only two motor units spanned all recording sites. The majority of the recorded motor units had muscle fibres located in two main rostro-caudal territories (32 motor units spanned L1 to L3 and 30 spanned ∼L3 to L4) and 11 had muscle fibres outside these two main territories. We also observed distinct muscle activation between the rostral and caudal regions of the longissimus pars lumborum during a trunk rotation task. Our results show clear rostral and caudal motor unit territories in the longissimus pars lumborum muscle and suggest that the central nervous system can selectively activate regions of the superficial lumbar muscles to provide local stabilization of the spine.

Soleus single motor units show stronger coherence with Achilles tendon vibration across a broad bandwidth relative to medial gastrocnemius units while standing

To probe the frequency characteristics of somatosensory responses in the triceps surae muscles, we previously applied suprathreshold noisy vibration to the Achilles tendon and correlated it with ongoing triceps surae muscle activity (recorded via surface EMG) during standing. Stronger responses to tendon stimuli were observed in soleus (Sol) relative to medial gastrocnemius (MGas) surface EMG; however, it is unknown whether differences in motor unit activity or limitations of surface EMG could have influenced this finding. Here, we inserted indwelling EMG into Sol and MGas to record the activity of single motor units while we applied noisy vibration (10-115 Hz) to the right Achilles tendon of standing participants. We analyzed the relationship between vibration acceleration and the spike activity of active single motor units through estimates of coherence, gain, phase, and cross-covariance. We also applied sinusoidal vibration at frequencies from 10 to 100 Hz (in 5-Hz increments) to examine whether motor units demonstrate nonlinear synchronization or phase locking at higher frequencies. Relative to MGas single motor units, Sol units demonstrated stronger coherence and higher gain with noisy vibration across a bandwidth of 7-68 Hz, and larger peak-to-peak cross-covariance at all four stimulus amplitudes examined. Sol and MGas motor unit activity was modulated over the time course of the sinusoidal stimuli across all frequencies, but their phase-locking behavior was minimal. These findings suggest Sol plays a prominent role in responding to disturbances transmitted through the Achilles tendon across a broad frequency band during standing.NEW & NOTEWORTHY We examined the relationship between Achilles tendon stimuli and spike times of single soleus (Sol) and medial gastrocnemius (MGas) motor units during standing. Relative to MGas, Sol units demonstrated stronger coherence and higher gain with noisy stimuli across a bandwidth of 7-68 Hz. Sol and MGas units demonstrated minimal nonlinear phase locking with sinusoidal stimuli. These findings indicate Sol plays a prominent role in responding to tendon stimuli across a broad frequency band.

Discharge properties of human diaphragm motor units with ageing.

Ageing is associated with changes in the respiratory system including in the lungs, rib cage and muscles. Neural drive to the diaphragm, the principal inspiratory muscle, has been reported to increase during quiet breathing with ageing. We demonstrated that low-threshold motor units of the human diaphragm recruited during quiet breathing have similar discharge frequencies across age groups and shorter discharge times in older age. With ageing, motor unit action potential area increased. We propose that there are minimal functionally significant changes in the discharge properties of diaphragm motor units with ageing despite remodelling of the motor unit in the periphery. There are changes in the skeletal, pulmonary and respiratory neuromuscular systems with healthy ageing. During eupnoea, one study has shown relatively higher crural diaphragm electromyographic activity (EMG) in healthy older adults (>51years) than in younger adults, but these measures may be affected by the normalisation process used. A more direct method to assess neural drive involves the measurement of discharge properties of motor units. Here, to assess age-related changes in neural drive to the diaphragm during eupnoea, EMG was recorded from the costal diaphragm using a monopolar needle electrode in participants from three age groups (n≥7 each): older (65-80years); middle-aged (43-55years) and young (23-26years). In each group, 154, 174 and 110 single motor units were discriminated, respectively. A mixed-effects linear model showed no significant differences between age groups for onset (group mean range 9.5-10.2Hz), peak (14.1-15.0Hz) or offset (7.8-8.5Hz) discharge frequencies during eupnoea. The motor unit recruitment was delayed in the older group (by ∼15% of inspiratory time; p=0.02 cf. middle-aged group) and had an earlier offset time (by ∼15% of inspiratory time; p=0.04 cf. young group). However, the onset of multiunit activity was similar across groups, consistent with no global increase in neural drive to the diaphragm with ageing. The area of diaphragm motor unit potentials was ∼40% larger in the middle-aged and older groups (P<0.02), which indicates axonal sprouting and re-innervation of muscle fibres associated with ageing, even in middle-aged participants.

Reorganization of Masseter and Temporalis Muscle Single Motor Unit Activity During Experimental Masseter Muscle Pain.

To test the hypothesis that experimental noxious stimulation of the right masseter muscle results in a reorganization of motor unit activity within the right temporalis and right masseter muscles during jaw closing tasks. A total of 20 healthy participants received hypertonic saline (5% sodium chloride) infusion into the right masseter muscle, and pain intensity was maintained at 40-60/100 mm on a visual analog scale. Standardized isometric biting tasks were performed with an intraoral force transducer while single motor units (SMUs) were recorded from the right masseter and temporalis muscles. Tasks were repeated in four blocks: block 1 (baseline 1), block 2 (hypertonic saline [HS] infusion or isotonic saline [IS] infusion), block 3 (infusion of the other solution), and block 4 (baseline 2). The occurrences of SMUs were tabulated across blocks. Statistical significance was considered to be P < .05. There were no significant effects of block on the tasks. A total of 83 SMUs were discriminated in the temporalis and 58 in the masseter. For the comparison between HS and IS across tasks, the occurrences of 74.6% to 82.8% of SMUs were unchanged (70.2% to 94.3% for masseter), while during HS, 10.3% to 17.1% of SMUs were recruited (0% to 12.8%, masseter) and 6.9% to 12.7% were de-recruited (5.7% to 17%, masseter). The present findings suggest that most biting-task-related jaw muscle SMUs remain active during experimental muscle noxious stimulation. There was some evidence in both the anterior temporalis and masseter muscles for motor unit recruitment and de-recruitment consistent with a motor unit reorganization during experimental pain.

Single motor units from the medial pterygoid muscle can be active during isometric horizontal and vertical forces.

To determine (a) whether the medial pterygoid muscle is active in an isometric vertical force task and in isometric horizontal force tasks in the contralateral, protrusion and ipsilateral directions; (b) whether the same single motor units (SMUs) could be active across different directions of isometric force generation; and (c) whether different regions of the medial pterygoid muscle exhibit different patterns of SMU activation during the generation of any one direction of isometric force. Intramuscular electromyographic (EMG) recordings were made from the right medial pterygoid muscle in 15 healthy participants during isometric force tasks: vertical and horizontal contralateral, protrusion and ipsilateral. A computed tomography scan divided the EMG recording site into a medial or lateral part in each participant. Single motor units were discriminated in each task. Medial pterygoid SMU activity was recorded in 100% of participants for the vertical biting tasks, 86% of participants for the horizontal contralateral and horizontal protrusion tasks and 57% of the horizontal ipsilateral tasks. Of the 72 SMUs that were discriminated, 36% were active in all tasks; 18% were active only in the vertical tasks and 17% were active in the vertical, horizontal contralateral and horizontal protrusion tasks. The proportion of SMUs that was active in at least 1 horizontal task in the lateral part (33/39) was significantly higher than the proportion (21/33) in the medial part (Chi-Square, P<0.05). The data are consistent with a stabilisation role for the medial pterygoid muscle in isometric jaw forces in the vertical and horizontal planes.

Exclusive Image Gallery on Human Spinal Cord Regeneration-Clinical Image-17

Layout for measuring coordination dynamics (arrhythmicity of exercising) between arm and leg movements, displayed on the laptop; for the intermediate coordination’s between pace and trot gait, the fluctuation of the network states is larger. The recording of sEMG activity (displayed on the oscilloscope) from the tibialis anterior and other muscles is also shown. The inset shows single motor unit action potentials on the lowest trace. The recordings are taken from a patient (Kadri) with a motoric complete cervical spinal cord injury C5/6 (95% injury).

Repetitive doublet firing in human motoneurons: evidence for interaction between common synaptic drive and plateau potential in natural motor control.

The firing behavior of spinal motoneurons (MNs) is a result of processing synaptic inputs by MN membrane properties, including plateau potentials, fundamentally explored in animals. However, there is much less data about a plateau potential role in human motor control. We explored human MN repetitive doublet firing during gentle isometric voluntary muscle contractions with the aim of revealing possible evidence for interaction between plateau potentials and common synaptic drive known as an important determinant of MN pool firing behavior. Single-motor unit (MU) repetitive firing of trapezius and triceps brachii was analyzed. Subjects were asked to recruit MUs capable of firing repetitive doublets. The analysis of interspike intervals (ISIs) of background firing of simultaneously recorded MUs showed that beyond doublet series ISIs varied, often in unison with significant correlation coefficients, demonstrating common synaptic drive. During doublet series, MUs showed persistent doublet ISIs (typically 4-7 ms) and a tendency to increase the number of doublets in series throughout the experiment. This was consistent with involvement of MN plateau potentials resulting in persistent delayed depolarization (underlying each doublet) and warm-up effect. Common synaptic drive "started" doublet series; probably both mechanisms controlled postdoublet ISIs. However, convincing effects of plateau potentials on MU firing behavior during single firing were not found. Thus our results suggest a plateau potential role in specifying the essential firing pattern, doubling, of some MUs rather than its effect on firing behavior of the MN pool, on the whole, during voluntary muscle contractions in humans. NEW & NOTEWORTHY Properties of human motoneuron repetitive doublet firing were explored during voluntary muscle contractions. It was shown for the first time that these properties seem to be consistent with properties of both plateau potentials, resulting in persistent delayed depolarization (underlying each doublet) and common synaptic drive, starting this unusual firing; both mechanisms could probably control postdoublet intervals. A convincing effect of plateau potentials on motoneuron single-spike firing, despite doublet firing, was not found.

P23-F Re-examining the origin of medium latency reflex response of soleus using single motor units

Background This study is inquiring the origin of the medium latency reflex response (MLR) that occurs in soleus muscle when common peroneal nerve is electrically stimulated. Material and methods In total, 23 SingleMUs from 9 males were obtained. During SMU recruitment, 308 ± 141 stimuli were delivered. We investigated the duration of the inhibitory postsynaptic potential (IPSP) and excitatory postsynaptic potential (EPSP). Results Common peroneal nerve stimulation resulted in M-response at SEMG of TA muscle and MLR in SOL-SEMG as well as accumulation of SMUs between 50 and 120 ms after the stimulus. A reduction in firing probability of SMUs was also observed and the reduction in discharge rate continued much longer than the spike reduction probability in PSTH and CUSUM. This reduction in discharge rate was followed by an increased discharge rate of SMUs. Duration of the IPSP obtained at PSF, 24.5 ± 6.3 ms, and the duration of EPSP, i.e. MLR, in PSF was found as 119.3 ± 54.3 The latency of EPSP was found higher in PSF and SEMG as 68.3 ± 9.9 ms and 74.5 ± 9.7 ms, than PSTH and MMU which were 56.3 ± 4.6 ms and 57.6 ± 4.2 ms, respectively. Conclusions There was a genuine Ia reciprocal inhibition followed by a stretch-induced reflex response in the SOL. The first response was due to the well-known Ia-IPSP pathway connecting antagonist muscles and the second response, the so-called MLR in the literature, was an indirect stretch reflex response caused by the M-response in the TA muscle. This Project (3135) was supported by Akdeniz University Scientific Research Unit .

P70-S Effect of motor unit firing rate on cutaneous silent period in a patient with ALS

Background Cutaneous silent period (CuSP) is a brief suppression of voluntary contraction that follows strong electrical stimulation of a cutaneous nerve. It is known that the level of voluntary contraction has little influence on the CuSP duration. On the other hand, in several experiments with single motor unit (MU) recordings it was shown that CuSP duration depends on the discharge rate of a single motor neuron. Materials and methods We have observed a 63-year-old woman with definite amyotrophic lateral sclerosis (ALS) according to El Escorial criteria, clinical signs of both upper and lower motor neuron involvement and bulbar onset. The number of functioning MUs in the right abductor pollicis brevis muscle estimated by adapted multi-point stimulation method showed very few remaining high-amplitude MUs. Several CuSP recordings were performed at different levels of voluntary contraction which were mostly provided by change of motor unit firing rate. Results CuSP duration varied little between different recordings. The onset latency was normal, but CuSP duration was increased significantly. At the same time, CuSP duration was similar between recordings with different motor unit discharge rates. Conclusion We suggest that intrinsic electrophysiological characteristics (e.g. effects of collateral reinnervation) of the remaining motor neurons may be the underlying cause of CuSP duration consistency regardless of MU firing rate. Lengthening of CuSP can be both associated with changes that motor neurons undergo during the course of the disease or inhibitory effects of supraspinal origin.

O-24 MRI detection of human motor unit fasciculation in Amyotrophic Lateral Sclerosis

Objective To develop a clinically applicable non-invasive method for detecting motor unit fasciculation in patients with Amyotrophic Lateral Sclerosis (ALS). Methods The lower limbs of 6 healthy controls and 4 patients with confirmed ALS were scanned using a novel diffusion weighted MRI protocol sensitive to micrometer-scale movement of skeletal muscle. Motor unit activity was assessed at rest and during electrical motor nerve stimulation time-locked to scanner acquisition. Results Incremental stimulation revealed a reproducible pattern of overlapping signal voids with dimensions and temporal profiles consistent with the contraction of single motor units. Patients with ALS showed a significantly higher rate of spontaneous motor unit fasciculation at rest (mean 99.1/min, range 25.7–161 in patients vs 7.7/min, range 4.3–9.7 in controls, p Conclusions This study is the first use of imaging to detect fasciculation in ALS patients. The technique is quick to perform and entirely pain-free. The ability to detect changes in motor unit function which precede motor unit degeneration may allow earlier diagnosis and recruitment to clinical trials.

Separation of interference surface electromyogram into propagating and non-propagating components

A new algorithm is introduced to decompose interference surface electromyogram (EMG) recorded by a multi-channel system aligned to muscle fibers into propagating and non-propagating contributions. Muscle fiber conduction velocity (CV) is also estimated, reducing the bias induced by non-propagating components. The algorithm is fast and stable, as it is based on alignment and averaging procedures. Simulated signals (with different fat thickness, SNR, number of channels, epoch duration and force level) are used to test the algorithm. The median cross-correlation of simulated and estimated components were about 98% and 90%, for propagating and non-propagating terms, respectively. CV was estimated better than using a multi-channel maximum likelihood approach applied to double differential data (mean error of 0.08 versus 0.13 m/s), with a greater gain in case of thinner fat layer, low SNR and few channels. Example applications to experimental data are also shown (single motor unit action potential, M-wave and interference EMG).Propagating components reflect the travelling of action potentials along muscle fibers. Preliminary tests show that non-propagating contributions provide selective information on motor units firing statistics. The separation of interference EMG into propagating and non-propagating components opens new perspectives, e.g., in the study of synergies, common drive and myoelectric manifestations of fatigue.

Activity of Motor Units in Human Elbow Flexor and Extensor Muscles during Task-Dependent Unloading

Surface EMGs and activity of 73 single motor units (MUs) of the m. triceps brachii caput laterale (TBcl) and 25 units of the m. biceps brachii caput longum (BBcl) were recorded in five adults during unloading within the ramp phase of the isotorque ramp-and-hold movements in the elbow joint (“movement” task) and during maintaining of a certain position (“position control” task). Analysis of the surface EMGs showed that patterns of the MU responses to unloading in different motor tasks are dissimilar. Depending on the responses to unloading, the examined MUs were classified into two groups. Motor units of the first group, including 14 TBcl MUs and 22 BBcl ones, were characterized as task-independent units. Their spiking was completely inhibited in response to unloading in both motor tasks. The second group (44 TBcl and 3 BBcl MUs) should be considered as a group of the task-dependent units. These MUs were active during unloading in the “movement” task but were fully inhibited in the “position control” task. This specificity in the reactions of MUs during movements may be related to their different functions. Presumably, the former group of MUs is mainly involved in the force control, while the latter one is associated with the control of the movement per se.

Medium latency excitatory reflex of soleus re-examined.

We aimed to study the receptor origin and postsynaptic potential profile of the medium latency reflex (MLR) response that develops in the soleus muscle when common peroneal nerve of antagonist tibialis anterior (TA) muscle is electrically stimulated. To achieve this aim, we electrically stimulated common peroneal nerve and recorded surface electromyography (SEMG) responses of soleus and TA muscles of informed volunteers. Additionally, we recorded intramuscular EMG from the soleus muscle. Stimulation of common peroneal nerve induced a direct motor response (M-response) in the TA and MLR in SEMG of the soleus. Using voluntarily-activated single motor units (SMUs) from the soleus muscle we noted that there were two distinct responses following the stimulus. The first response was a reciprocal inhibitory reflex probably originating from the antagonist muscle spindle primary (Ia) afferents. This was followed by an indirect reflex response activated by the contraction of the TA muscle during the M-response. This contraction generated a rapid acceleration in the direction of dorsiflexion hence inducing a stretch stimulus on soleus muscle. The response of soleus to this stimulus was a stretch reflex. We suggest that this stretch reflex is the main contributor to the so-called soleus MLR in the literature. This study illustrated the importance of using SMUs and also using discharge-rate based analysis for closely examining previously 'established' reflexes.

Sound-evoked vestibular projections to the splenius capitis in humans: comparison with the sternocleidomastoid muscle.

The short-latency vestibulo-collic reflex in humans is well defined for only the sternocleidomastoid (SCM) neck muscle. However, other neck muscles also receive input from the balance organs and participate in neck stabilization. We therefore investigated the sound-evoked vestibular projection to the splenius capitis (SC) muscles by comparing surface and single motor unit responses in the SC and SCM muscles in 10 normal volunteers. We also recorded surface responses in patients with unilateral vestibular loss but preserved hearing and hearing loss but preserved vestibular function. The single motor unit responses were predominantly inhibitory, and the strongest responses were recorded in the contralateral SC and ipsilateral SCM. In both cases there was a significant decrease or gap in single motor unit activity, in SC at 11.7 ms for 46/66 units and in SCM at 12.7 ms for 51/58 motor units. There were fewer significant responses in the ipsilateral SC and contralateral SCM muscles, and they consisted primarily of weak increases in activity. Surface responses recorded over the contralateral SC were positive-negative during neck rotation, similar to the ipsilateral cervical vestibular evoked myogenic potential in SCM. Responses in SC were present in the patients with hearing loss and absent in the patient with vestibular loss, confirming their vestibular origin. The results describe a pattern of inhibition consistent with the synergistic relationship between these muscles for axial head rotation, with the crossed vestibular projection to the contralateral SC being weaker than the ipsilateral projection to the SCM. NEW & NOTEWORTHY We used acoustic vestibular stimulation to investigate the saccular projections to the splenius capitis (SC) and sternocleidomastoid (SCM) muscles in humans. Single motor unit recordings from within the muscles demonstrated strong inhibitory projections to the contralateral SC and ipsilateral SCM muscles and weak excitatory projections to the opposite muscle pair. This synergistic pattern of activation is consistent with a role for the reflex in axial rotation of the head.

Transitory force decrease following a sudden reduction in stimulation frequency in motor units of rat medial gastrocnemius

Effects of a sudden decrease in the stimulation frequency for motor unit force were studied in rat medial gastrocnemius. For 161 functionally isolated single motor units of three types (S, FR, FF), unfused tetanic contractions were evoked by three-phase trains of stimuli (low–high–low frequency). The course of the tetanus at the onset of the third phase of the force recording was analyzed in tetani with variable fusion degree. For 78 units within the third phase of tetanus, a transitory force decrease to a level lower than in the first phase (identical frequency), was observed. This phenomenon was more frequent for fast fatigue resistant (65.9%) than for fast fatigable and slow motor units (27.1% and 35.5%, respectively). Moreover, the force decrease was strongest for fast resistant motor units (up to 36.5%) and when contractions evoked at variable frequencies of stimulation were compared, the highest amplitudes of the studied force decrease were noted for middle-fused tetani (0.50–0.90). A new phenomenon of transitory force decrease in tetanic contractions of motor units with a decrease in stimulation frequency was found. Most probably, the phenomenon is dependent on disturbances in the force transmission by collagen surrounding active muscles fibers.

Oscillations in neural drive and age-related reductions in force steadiness with a cognitive challenge.

A cognitive challenge when imposed during a low-force isometric contraction will exacerbate sex- and age-related decreases in force steadiness, but the mechanism is not known. We determined the role of oscillations in the common synaptic input to motor units on force steadiness during a muscle contraction with a concurrent cognitive challenge. Forty-nine young adults (19-30 yr; 25 women, 24 men) and 36 old adults (60-85 yr; 19 women, 17 men) performed a cognitive challenge (counting backward by 13) during an isometric elbow flexion task at 5% of maximal voluntary contraction. Single-motor units were decomposed from high-density surface EMG recordings. For a subgroup of participants, motor units were matched during control and cognitive challenge trials, so the same motor unit was analyzed across conditions. Reduced force steadiness was associated with greater oscillations in the synaptic input to motor units during both control and cognitive challenge trials ( r = 0.45-0.47, P < 0.01). Old adults and young women showed greater oscillations in the common synaptic input to motor units and decreased force steadiness when the cognitive challenge was imposed, but young men showed no change across conditions (session × age × sex, P < 0.05). Oscillations in the common synaptic input to motor units is a potential mechanism for altered force steadiness when a cognitive challenge is imposed during low-force contractions in young women and old adults. NEW & NOTEWORTHY We found that oscillations in the common synaptic input to motor units were associated with a reduction in force steadiness when a cognitive challenge was imposed during low-force contractions of the elbow flexor muscles in young women and old men and women but not young men. Age- and sex-related muscle weakness was associated with these changes.

Motor units as tools to evaluate profile of human Renshaw inhibition.

To uncover the synaptic profile of Renshaw inhibition on motoneurons, we stimulated thick motor axons and recorded from voluntarily-activated motor units. Stimuli generated a direct motor response on the whole muscle and an inhibitory response in active motor units. We have estimated the profile of Renshaw inhibition indirectly using the response of motor unit discharge rates to the stimulus. We have put forward a method of extrapolation that may be used to determine genuine synaptic potentials as they develop on motoneurons. These optimized techniques can be used in research and in clinics to fully appreciate Renshaw cell function in various neurological disorders. Although Renshaw inhibition (RI) has been extensively studied for decades, its precise role in motor control is yet to be discovered. One of the main handicaps is a lack of reliable methods for studying RI in conscious human subjects. We stimulated the lowest electrical threshold motor axons (thickest axons) in the tibial nerve and analysed the stimulus-correlated changes in discharge of voluntarily recruited low-threshold single motor units (SMUs) from the soleus muscle. In total, 54 distinct SMUs from 12 subjects were analysed. Stimuli that generated only the direct motor response (M-only) on surface electromyography induced an inhibitory response in the low-threshold SMUs. Because the properties of RI had to be estimated indirectly using the background discharge rate of SMUs, its profile varied with the discharge rate of the SMU. The duration of RI was found to be inversely proportional to the discharge rate of SMUs. Using this important finding, we have developed a method of extrapolation for estimating RI as it develops on motoneurons in the spinal cord. The frequency methods indicated that the duration of RI was between 30 and 40ms depending on the background firing rate of the units, and the extrapolation indicated that RI on silent motoneurons was ∼55ms. The present study establishes a novel methodology for studying RI in human subjects and hence may serve as a tool for improving our understanding of the involvement of RI in human motor control.

Positive–Negative Stiffness Actuators

Compliant actuators are typically designed to possess a tunable positive stiffness characteristic in order to generate restoring force upon displacement. These actuators either require two independent motor units or closed-loop control to change both their equilibrium position and output stiffness. The introduction of negative stiffness, in combination with tunable positive stiffness, may reduce the complexity and extend the capability of these actuators in unexpected ways. In this paper, we present a compliant actuator that employs a passive negative stiffness mechanism in conjunction with an effectively tunable positive stiffness mechanism. We show that such actuator enables open-loop stiffness modulation and equilibrium position control using a single motor unit, as opposed to more conventional variable stiffness and series elastic actuators. The paper presents the theoretical foundation of positive-negative stiffness actuators and demonstrates low-power stiffness modulation and equilibrium position control achievable with a prototype positive-negative stiffness actuator.