Background: Transesophageal echocardiography (TEE) is often considered as the reference method for the measurement of the aortic annulus diameter (AAD) during transcatheter aortic valve implantation (TAVI). In the present study, we evaluated whether Multislice Computed Tomography (MSCT) could reproduce and thus substitute to TEE. Method: We compared AAD measurements performed using TEE, MSCT and transthoracic echocardiography (TTE) in 129 consecutive patients with severe aortic stenosis (AS) referred for TAVI. Using MSCT, AAD was measured in the 3-chamber view and at the level of the virtual basal ring. We calculated the mean (MD) of the long-axis (LA) and short-axis (SA) diameters, mathematic transformations emphasizing the weight of the SA (MD2 to MD5, MD2 = 2SA + LA/3 ...), AAD derived from the cross-sectional area and from the circumference of the virtual basal ring. Comparisons between methods were assessed using single-measure intraclass correlation coefficient (ICC) and agreements as regard to the TAVI strategy (decision to implant and choice of the prosthesis' size based on manufacturer's cutoffs recommendations) expressed using the kappa value. Results: The 3C method (ICC=0.79, 95% interval 0.73-0.83), MD4 (ICC=0.76, 95% interval 0.69-0.81) and MD5 (ICC=0.75, 95% interval 0.67-0.81) provided the highest correlation and the best agreement to TEE (kappa value of 0.47, 0.27 and 031 respectively) but remained lower than TTE (ICC=0.87; 95% interval 0.83-0.91; kappa=0.66). The agreement between MSCT and TEE varied with the degree of eccentricity of the aortic annulus or the degree of aortic valve calcification but never reached the values observed with TTE. Conclusion: No direct or indirect MSCT method provided higher correlations to TEE than TTE. Consequently, no MSCT method could reproduce and thus substitute to TEE. Randomized prospective studies are clearly needed to evaluate which method provides the best clinical results, but we definitely demonstrate that MSCT and TEE are not interchangeable.