Background/Objectives: Physical fitness in youth soccer impacts individual and team performance through the specific demands that must be met on the field. Therefore, this study aimed to characterize and identify youth soccer players with regard to the roles they play on the field. Material and Methods: A cross-sectional study was designed to characterize and identify the physical fitness levels of youth soccer players using previously validated measurement tests. A total of 36 players were evaluated (15 defenders and 24 attackers) using various physical fitness tests: Squat Jump (SJ), Countermovement Jump (CMJ), Single-leg Countermovement Jumps (SLCMJs), COD-Timer 5-0-5, Speed (5, 10, 15, and 20 m), Yo-Yo Intermittent Recovery Test Level I (YYIR1), and Running-Based Anaerobic Sprint Test (RAST). The data were confirmed using the Shapiro–Wilk test. Effect sizes were obtained using the Rank-Biserial coefficient, and, to identify the profiles of attackers and defenders, principal component analysis (PCA) was employed. Results: For the strength variables, attackers obtained better results than defenders in the variable flight time in the SJ (p = 0.03; R-b = −0.33) and contact time (%) in the SLCMJ test (p = 0.04; R-b = −0.33). Meanwhile, defenders achieved better results than attackers in the SLCMJ test for the variable flight time (%) (p = 0.01; R-b = 0.33) and breaking angle (A°) in the Nordic Hamstring (p = 0.01; R-b = 0.33). The results showed differences according to the players’ roles. Three principal components were identified for both attackers and defenders. The PC1 for attackers considered variables of strength, asymmetry, change of direction, and power. PC2 only considered strength and power variables. PC3 considered variables of strength, speed, endurance, and power. For defenders, PC1 considered strength, asymmetry, and power. PC2 analyzed variables of strength, asymmetry, change of direction and power. Finally, PC3 only grouped speed variables. Conclusions: Although youth soccer positions involve offensive and defensive roles, this study reveals differences in certain physical fitness variables. Therefore, it is necessary to tailor training tasks according to the specificity of the playing position, in line with the systems of play used and the predominance of the role that players occupy, whether in defense or attack.
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