Single Hair Research Articles

A Soybean Acyl Carrier Protein, GmACP, Is Important for Root Nodule Symbiosis

Legumes (members of family Fabaceae) establish a symbiotic relationship with nitrogen-fixing soil bacteria (rhizobia) to overcome nitrogen source limitation. Single root hair epidermal cells serve as the entry point for bacteria to infect the host root, leading to development of a new organ, the nodule, which the bacteria colonize. In the present study, the putative role of a soybean acyl carrier protein (ACP), GmACP (Glyma18g47950), was examined in nodulation. ACP represent an essential cofactor protein in fatty acid biosynthesis. Phylogenetic analysis of plant ACP protein sequences showed that GmACP was classified in a legume-specific clade. Quantitative reverse-transcription polymerase chain reaction analysis demonstrated that GmACP was expressed in all soybean tissues but showed higher transcript accumulation in nodule tissue. RNA interference-mediated gene silencing of GmACP resulted in a significant reduction in nodule numbers on soybean transgenic roots. Fluorescent protein-labeled GmACP was localized to plastids in planta, the site of de novo fatty acid biosynthesis in plants. Analysis of the fatty acid content of root tissue silenced for GmACP expression, as determined by gas chromatography-mass spectrometry, showed an approximately 22% reduction, specifically in palmitic and stearic acid. Taken together, our data provide evidence that GmACP plays an important role in nodulation.

MiR‐424 levels in hair shaft are increased in psoriatic patients

Objective diagnostic markers have not been in clinical use for psoriasis. In this study, we investigated the levels of miR-424 in hair roots and hair shafts in psoriatic patients, and evaluated the possibility that miR-424 can be a biomarker of the disease. A single hair root and five pieces of hair shafts (~5cm in length) were obtained from the non-lesional occiput of each individual of 26 psoriatic patients. Control hair samples were collected from nine normal subjects. Samples from 10 atopic dermatitis patients were also included as the disease control. miR-424 levels were determined by quantitative real-time polymerase chain reaction. Hair shaft miR-424 levels were significantly upregulated only in patients with psoriasis compared with normal controls and those with atopic dermatitis. By receiver-operator curve analysis of hair shaft miR-424 to distinguish psoriatic patients from normal subjects, the area under the curve was 0.77. However, relative miR-424 levels were not correlated with disease activity markers including disease duration, body surface area and Psoriasis Area and Severity Index. Hair root miR-424 was not useful for evaluating both diagnosis and severity of the disease. Our results indicated hair shaft miR-424 levels may be useful as a diagnostic marker of psoriasis.

A SNP chip to detect introgression in wildcats allows accurate genotyping of single hairs

Genotyping non-invasively collected samples is challenging. Nevertheless, genetic monitoring of elusive species like the European wildcat (Felis silvestris silvestris) mainly relies on such samples. Wildcats are likely threatened through introgression with domestic cats (F. silvestris catus). To determine introgression based on single cat hairs, we developed a 96.96 Fluidigm single nucleotide polymorphism (SNP) genotyping array chip. To estimate the accuracy of this method, we compared genotypes of 17 cats called with both Sanger sequencing and Fluidigm. When Sanger sequencing genotypes were considered as a reference, the genotyping error rate with Fluidigm was 0.9 %. We subsequently compared 16 hair samples to tissue samples of the same individual. When the tissue samples were used as a reference, the genotyping error rate in hair samples was 1.6 %. This low error rate allowed reliable recognition of individuals and correct assessment of introgression levels. Thus, the genotyping method presented in this paper is suitable for non-invasively collected samples. It will help conservationists to monitor the introgression rate in wildcat populations based on non-invasive hair sampling and subsequently to conduct effective conservation measures.

Durability and field condition study of seal of SAVY-4000 storage container

The conditions of use for rubber O-rings are at least as important as their physical properties in their effect on the quality of a seal. Under normal use conditions, O-rings may be subject to wear and environmental contaminants such as hair and dirt. This study examines how these factors impact the leak tightness of a nuclear material storage container and the likelihood of the inadvertent release of radioactive material. The durability lifetime of an O-ring was explored by opening and closing four SAVY-4000 1 quart containers 100 times and periodically performing helium leak testing, though no significant change in leak rate was observed. This study also explored how the accumulation of dust or hair on the O-ring surface would affect the leak rate of the containers. A single hair crossing the seal, or a sufficient amount of particulate matter would compromise the seal, but after cleaning, the seal was re-established.

The biasing influence of linguistic variations in DNA profiling evidence

Extensive psychological research has confirmed that probabilistic statistical information that is mathematically equivalent, if presented in different linguistic forms, is not psychologically equivalent. Commentators have argued that certain forms of evidence are more prejudicial than probative and should be excluded from trial. In a recent homicide case based on circumstantial evidence, the sole evidence linking a suspect to crime scene was mitochondrial DNA profiling evidence derived from a single loose hair found at the crime scene. Variations in the form of the linguistic evidence presented by DNA forensic scientists and research about its differential impact were cited as the basis for appeal of the conviction to the Australian High Court. A review of the research revealed that presentation of statistical information using natural frequencies is a recommended best practice. Random match probabilities expressed as frequencies are less susceptible of misinterpretation by legal professionals and lay jurors, and lead to fewer false convictions.

A reduction in Ptprq associated with specific features of the deafness phenotype of the miR‐96 mutant mouse diminuendo

miR-96 is a microRNA, a non-coding RNA gene which regulates a wide array of downstream genes. The miR-96 mouse mutant diminuendo exhibits deafness and arrested hair cell functional and morphological differentiation. We have previously shown that several genes are markedly downregulated in the diminuendo organ of Corti; one of these is Ptprq, a gene known to be important for maturation and maintenance of hair cells. In order to study the contribution that downregulation of Ptprq makes to the diminuendo phenotype, we carried out microarrays, scanning electron microscopy and single hair cell electrophysiology to compare diminuendo mutants (heterozygous and homozygous) with mice homozygous for a functional null allele of Ptprq. In terms of both morphology and electrophysiology, the auditory phenotype of mice lacking Ptprq resembles that of diminuendo heterozygotes, while diminuendo homozygotes are more severely affected. A comparison of transcriptomes indicates there is a broad similarity between diminuendo homozygotes and Ptprq-null mice. The reduction in Ptprq observed in diminuendo mice appears to be a major contributor to the morphological, transcriptional and electrophysiological phenotype, but does not account for the complete diminuendo phenotype.

A Rare Cause of a Pressure Ulcer: A Single Hair

Pressure ulcers are the loss of tissue and remain a common problem in critically ill patients. These patients are at high risk for developing pressure ulcers because of their hemodynamic instability, malnutrition, and strictly limited physical activity and mobility due to neurological damage or drugs such as sedatives and muscle relaxants. Pressure is the most important cause of pressure ulcers. Contact surfaces such as patient’s bed or wheelchair, and some medical devices such as pulse oximetry probe and urethral catheter can result in pressure. The case is reported here of a very rare cause of a pressure ulcer, which was a single hair on the patient’s face.

Simultaneous determination of trace cadmium and lead in single human hair by tungsten electrothermal vaporization-flame atomic fluorescence spectrometry

A new method was proposed for simultaneous determination of ultratrace cadmium and lead in a single string of human hair by coupling of a tungsten coil electrothermal vaporizer (W-coil ETV) and an argon–hydrogen flame for atomic fluorescence spectrometry (Ar–H2 flame AFS). The sample digestion and experimental conditions for the determination of cadmium and lead in a single hair were investigated in detail. Under the optimal conditions, the limits of detection (LODs, 3σ) were 0.05pg and 3pg for Cd and Pb, respectively. The accuracy of the proposed method was demonstrated by analyzing a certified reference hair sample and several adult hair samples for trace Cd and Pb. By use of its high sensitivity and low sample consumption (typically 10μL), it is potentially useful for determination of trace elements in a single string of hair from career and crime scenes for forensic purpose.

Segmented Heterochromia in a Single Scalp Hair

Segmented Heterochromia in a Single Scalp Hair

A correlation of breast cancer and calcium levels in hair analyzed by X-ray fluorescence.

Time variations of elemental concentrations and their abnormalities due to breast cancer have been observed along single hair strands by X-ray fluorescence excited by synchrotron radiation. The renal-controlled elements Ca, Sr, S, K, Cl, Br and P have upper and lower levels associated with gating and closing of ion channels in the hair-making cells. The Ca lower level is normal. In cases of Ca deficiency, with a decrease from the normal, store-operated Ca channel gating occurs so as to keep the hair Ca at the normal, and paradoxically high Ca levels near or at the upper level are produced by PTH-operated channel gating of the cells. Chronic Ca deficiency shows a temporal pattern along the hair consisting of a long-term duration of the upper [Ca] level, 10-month long decay to the lower level and abrupt increase to the upper level. The observation for hair from breast-cancer patients also shows the upper Ca level for the time period well before detection, and suggests that cancer is always generated at the long-lasting [Ca] upper level and the hair [Ca] decreases gradually toward the lower level with the cancer growth. This decay of [Ca] is accompanied by those of [Sr] and [K]. Their different decay forms can be explained by parathyroid hormone related peptide (PTHrP) in serum secreted from the cancer having 150 times longer dwell time on the PTH receptors than that of PTH. Patient hair has a memory for the entire cancer process from the state before cancer generation, and the pattern can be distinguished from concentration variation due to the chronic Ca deficiency without cancer, leading to a criterion for cancer detection by the ratio of [Sr]/[Ca]. The hair analysis is useful for early detection of cancer.

Interpretation of hair findings in children: about a case involving carbamazepine

This office has been recently involved in a case dealing with child custody, where the final outcome was difficult to establish. The following concentrations were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in the hair of a 21-month-old girl: 154 (0-1 cm), 198 (1-2 cm), 247 (2-3 cm), and 368 pg/mg (3-4 cm) after decontamination. Obviously, the concentrations measured in the hair were much lower than those observed in patients under daily treatment. In this sense, the frequency of exposures appears as infrequent (low level of exposure), with marked decrease in the more recent period. However, the girl was never prescribed carbamazepine and the mother, who was under carbamazepine therapy, denied any administration. The Judge asked if this could result from a single exposure and at which period. At least, three possible interpretations of the measured carbamazepine concentrations were addressed: (1) decrease in administration in the more recent period; (2) increase of body weight due to growing, so the same dosage will result in lower concentrations in hair; and (3) sweat contamination from the mother at the time the girl is with her in bed, the older hair being in contact longer with the bedding. In this case, it was impossible to conclude that the child was deliberately administered carbamazepine. The results of the analysis of hair could indicate that she was in an environment where carbamazepine was being used and where the drug was not being handled and stored with appropriate care. There are many differences between the hair from children and those from adults: hair from children is thinner and more porous, the ratio anagen and catagen phases are not maintained, and the growth rate can be different, at some periods, from the usual 1 cm/month. These differences, together with the influence of PK-PD parameters are reviewed in this paper, as a basis for suitable interpretation. In view of these results it is proposed that a single hair analysis should not be used firmly to discriminate long-term exposure to a drug when dealing with children.

Studies in the liverwort family Metzgeriaceae (Metzgeriales) from southern Africa. 1. Metzgeria nudifrons Stephani and a key to the species

AbstractThis is the first of several projected articles on the taxonomy of southern African Metzgeriaceae H. Klinggr. A brief history of the genus Metzgeria Raddi in southern Africa, the only local representative, is presented. Family and genus descriptions, as well as a key to the species, are provided. Of the seven species currently recognized in southern Africa, M. nudifrons is the only species with gemmae on the dorsal surface of the thallus. It is also characterized by the distinctly broad costa, hairy male branchlets as well as single and straight hairs on the ventral face and margins of the thallus. Metzgeria nudifrons is described, illustrated and its geographical distribution mapped.

Evaluation of human hairs with terahertz wave

Single human hairs using a scanning laser terahertz (THz) imaging system are evaluated. The system features near-field THz emission and far-field THz detection. A sample is set in the vicinity of a two-dimensional THz emitter, and an excitation laser beam is scanned over the emitter via a galvanometer. By detecting the transmitted THz wave pulses that are locally generated at the irradiation spots of the excitation laser, we can obtain the THz transmission image and the spectrum of the sample with imaging time of 47 s for 512×512 pixels and maximum resolution of ∼27 μm . Using the system, we succeeded in observing the specific features of single human hairs in both the THz transmittance spectra and transmission images; it was found that the THz transmittance spectrum of gray hair shows a tendency of increase while that of black hair shows a decrease with increasing frequency above 1.2 THz. We could also observe the change of the moisture retention in the hair, and it is found that cuticles play one of the important roles in keeping moisture inside the hair. Those obtained data indicate that our system can be useful for evaluating single human hairs and those kinds of microscale samples.

Simple segmental hair analysis for α-pyrrolidinophenone-type designer drugs by MonoSpin extraction for evaluation of abuse history

For detection of a history of drug abused, we developed a simple method for extracting pyrrolidinophenone-type designer drugs in human hair by using a MonoSpin(®) C18 column. Target drugs were extracted from a single alkaline-digested hair segment (length, 10mm; weight, ca 0.1mg). The analytes extracted were then analyzed by high-performance liquid chromatography-mass spectrometry without evaporation of the eluent after MonoSpin extraction. Linearity from 0.5 to 500ng/mg was observed for all the tested drugs using an internal standard method (correlation coefficients >0.998) and the limit of detection was 0.2ng/mg. The recoveries were between 0.7 and 11.1%. The coefficients for intraday and interday variations at 4, 40, 200, and 400ng/mg in hair were between 0.7 and 11.1%. This method was successfully applied to the identification of these designer drugs in segmented human hair from drug abusers and indicated their history of drug abuse. The results were consistent with the patients' statements, indicating that this rapid method can be used to detect a history of drug abuse.

Creative Translation in Medieval Catalan: Bernat Metge

Creativity in the translation of a literary work is as difficult to quantify as it is to define. Insofar as inuentio, dispositio, and elocutio play a part in such a process, the result is bound to ...

Screening and long‐term retrospection for psychoactive drugs in presumptive drug‐facilitated crimes using segmented single hairs

Screening and long‐term retrospection for psychoactive drugs in presumptive drug‐facilitated crimes using segmented single hairs

Detection of hair-microRNAs as the novel potent biomarker: Evaluation of the usefulness for the diagnosis of scleroderma

BackgroundSerum microRNA levels are known as useful biomarkers for various diseases. Recent publication has indicated the existence of microRNAs in hair roots and hair shafts. ObjectiveIn this study, we evaluated several methods for the extraction of hair microRNAs, and their usefulness for the diagnosis of scleroderma. MethodsA single hair root and 5 pieces of hair shafts were obtained from the occiput of each individual of 11 scleroderma patients and 13 normal subjects at the time of serum sampling.microRNA extraction from sera or hair roots was performed with commercially available kits. microRNAs were extracted from hair shafts using four different methods. microRNA expression was evaluated by PCR array and real-time PCR. ResultsWe demonstrated microRNAs in hair roots and hair shafts were detectable and quantitative using our method. We found the difference of microRNA levels in hair roots and hair shafts obtained from different places of head in each individual were within 2-fold, indicating the reproducibility of hair microRNA levels by our method. PCR array revealed microRNAs from sera, hair roots and hair shafts have different expression pattern, and can be independent biomarkers.Serum and hair root miR-196a levels were not significantly changed in scleroderma patients, while we found miR-196a levels in hair shafts were significantly decreased in scleroderma patients compared to those in normal subjects (p<0.05). ConclusionHairs are more accessible than sera among human samples. microRNAs levels in hair roots or hair shafts may become effective and independent biomarkers.

The Co-Expression Pattern of Odorant Binding Proteins and Olfactory Receptors Identify Distinct Trichoid Sensilla on the Antenna of the Malaria Mosquito Anopheles gambiae

The initial steps of odorant recognition in the insect olfactory system involve odorant binding proteins (OBPs) and odorant receptors (ORs). While large families of OBPs have been identified in the malaria vector A. gambiae, little is known about their expression pattern in the numerous sensory hairs of the female antenna. We applied whole mount fluorescence in Situ hybridization (WM-FISH) and fluorescence immunohistochemistry (WM-FIHC) to investigate the sensilla co-expression of eight A. gambiae OBPs (AgOBPs), most notably AgOBP1 and AgOBP4, which all have abundant transcripts in female antenna. WM-FISH analysis of female antennae using AgOBP-specific probes revealed marked differences in the number of cells expressing each various AgOBPs. Testing combinations of AgOBP probes in two-color WM-FISH resulted in distinct cellular labeling patterns, indicating a combinatorial expression of AgOBPs and revealing distinct AgOBP requirements for various functional sensilla types. WM-FIHC with antisera to AgOBP1 and AgOBP4 confirmed expression of the respective proteins by support cells and demonstrated a location of OBPs within sensilla trichodea. Based on the finding that AgOBP1 and AgOBP4 as well as the receptor type AgOR2 are involved in the recognition of indole, experiments were performed to explore if the AgOBP-types and AgOR2 are co-expressed in distinct olfactory sensilla. Applying two-color WM-FISH with AgOBP-specific probes and probes specific for AgOR2 revealed a close association of support cells bearing transcripts for AgOBP1 and AgOBP4 and neurons with a transcript for the receptor AgOR2. Moreover, combined WM-FISH/-FIHC approaches using an AgOR2-specific riboprobe and AgOBP-specific antisera revealed the expression of the “ligand-matched” AgOBP1, AgOBP4 and AgOR2 to single trichoid hairs. This result substantiates the notion that a specific response to indole is mediated by an interplay of the proteins.

Phase structure of higher spin black hole

In this paper, we investigate the phase structures of the black holes with one single higher spin hair, focusing specifically on the spin 3 and spin tilde 4 black holes. Based on dimensional analysis and the requirement of having consistent thermodynamics, we derive an universal formula relating the entropy and the conserved charges for arbitrary AdS3 higher spin black holes. Then we use it to study the phase structure of the higher spin black holes. We find that there are six branches of solutions in the spin 3 gravity, eight branches of solutions in the spin tilde 4 gravity and twelve branches of solutions in the G2 gravity. In each case, all branches are related by a simple angle shift in the entropy functions. In the spin 3 case, we reproduce all the results found before. In the spin tilde 4 case, we find that in the low temperature it is at the BTZ branch while in the high temperature it transits to one of two other branches, depending on the signature of the chemical potential, a reflection of charge conjugate asymmetry found before.

Determination of cocaine, cocaine metabolites and cannabinoids in single hairs by MALDI Fourier transform mass spectrometry – preliminary results

In a preliminary test, four single hairs of a drug abuser were analyzed for the presence of drugs by MALDI Fourier transform mass spectrometry (MALDI-FTMS). Washed hair strains were directly fixed on a sample plate using pressure stable, double-sided adhesive tape; α-cyano-4-hydroxycinnamic acid matrix was manually spotted onto the hair strains. FTMS full scans were obtained moving from the hair root region towards the hair tip. Cocaine (accurate m/z ratio 304.15433) was identified mostly from the root of the hair and then later again towards the hair tip. This was confirmed by analysis of a second hair. Additionally cocaine metabolites with m/z ratio 290.13868 (benzoylecgonine), and m/z 318.16998 (cocaethylene) were detected for plausibility control. Using the MALDI technique, time-related information was obtained concerning the behavioural pattern of the consumer with high resolution compared to conventional procedures. However, in two hairs of the same individual which were analyzed under the same conditions, negative results were achieved. These preliminary results confirm the applicability of MALDI-MS for the determination of drugs and pharmaceuticals in hair samples being useful in forensic toxicology. The high chronological resolution allows an enhanced interpretation concerning the periods of drug administration. However, the negative results with two negative hairs have also demonstrated that hair analysis of single hairs can lead to misinterpretation. Different growth rates have to be considered, and particularly the phenomenon of different growth phases (anagen, catagen, telogen) require attention.