Single Factor Method Research Articles

Low Fatization of High-Fat Surimi-Based Products: Optimization of the Application of Protein Matrix Fat Substitution Methods.

The low fatization of high-fat foods is a significant trend that will impact the future developments of food products. Consumers have regarded health attributes as a critical indicator for purchasing food. In this study, enzyme-modified soy protein isolate, sea fish collagen, and ovalbumin were used to prepare the composite fat substitute for the protein matrix. This matrix was applied to the traditional surimi-based product Nemipterus virgatus fish sausage to replace the exogenous fat, and a new type of low-fat fish sausage was developed. This change is expected to reduce the exogenous fat in the traditional fish sausage without reducing the flavor and sensory quality of the original product. The results showed that taking the sensory evaluation and gel strength value of the product as indicators, the optimal ratio of compound fat substitute (enzyme-modified soy protein isolate:sea fish collagen:ovalbumin) was 2:1:3 when using the orthogonal test method for the first time. In the next step, with compound fat substitutes, exogenous fats and transglutaminase as the main factors, single factor and response surface method were used to explore the best formula of new low-fat Nemipterus virgatus fish sausage. The results showed that the best gel strength and sensory evaluation scores were obtained when the compound fat substitute, TGase, and exogenous fat were 0.59 g, 0.245 g, and 8.03 g, respectively. The optimal formulation of the low-fat Nemipterus virgatus fish sausage was obtained as follows: surimi, 67.52%; complex fat substitute, 0.66%; TGase, 0.28%; fat, 9.04%; starch, 6.75%; sugar, 3.94%; salt, 2.25%; monosodium glutamate, 0.23%; I&G, 0.34%; and water, 9%. Compared with the traditional fish sausage, the content of exogenous fat in the new, low-fat Nemipterus virgatus fish sausage was reduced by 54.8%. Meanwhile, the sensory score of fish sausage was increased by 21.79%, maintaining its good flavor and sensory quality. This study provides an important reference value for developing new low-fat surimi-based products.

Entrepreneurial education and intention: fear of failure, self-efficacy and gender

PurposeThis research adopts the social cognitive career theory (SCCT) and a moderated mediation model to investigate the moderating impacts of entrepreneurial fear of failure (FOF) and gender on the direct and mediation relationships between entrepreneurial education (EE), entrepreneurial self-efficacy (ESE) and entrepreneurial intention (EI).Design/methodology/approachThe authors utilized a three-phase random sampling to compile a dataset from 1,890 graduate students from nine universities and higher education institutions in Vietnam. Cronbach's alpha and confirmatory factor analysis results showed that the key study variables were reliable and valid. Harman's single-factor method and other tests of analysis assumptions ruled out common method bias and other confounding factors. The authors utilized the PROCESS macro to test a hypothesized moderated mediation model that included direct, indirect and conditional indirect effects.FindingsThe findings yield that ESE partially and positively mediates the relation between EE and EI. FOF was found to negatively moderate the impacts of EE on ESE and EI, and the direct effect of ESE on EI among females is stronger than among males. More importantly, the mediation influence of FOF on the linkage between EE and EI becomes weaker when the level of FOF is high, yet this mediation relationship among females is higher than among males at all levels of FOF.Practical implicationsThe results of this research are valuable for educators, policymakers and practitioners so that they may inspire individuals' entrepreneurial pursuits, especially those of female entrepreneurs.Originality/valueThis study significantly contributes to the entrepreneurship and gender literature by applying the SCCT to elucidate the moderated mediation impacts of FOF, ESE and gender on the relationship between EE and EI.

Experimental and numerical investigations on the thermal performance enhancement of a latent heat thermal energy storage unit with several novel snowflake fins

This article introduces a novel snowflake fin inspired by the crystal structure of snowflake to enhance the heat transfer performance of Latent Heat Thermal Energy Storage (LHTES) units. Initially, a numerical simulation model for transient heat transfer units with snowflake fin was established in a shell and tube LHTES unit. The accuracy and dependability of the model are verified through comparison with experimental results. Then, the effects of three placement methods of LHTES unit (vertical, horizontal-Ⅰ, and horizontal-Ⅱ) on melting and solidification performance are explored. Additionally, the effects of traditional longitudinal fins, snowflake fin, and four novel snowflake fins on melting and solidification behaviors are quantitatively compared. The single-factor method and response surface methodology (RSM) were employed to further optimize the selected optimal novel snowflake fin design. The research results show that the heat transfer performance of the vertically placed LHTES unit is the best. In comparison to traditional longitudinal fins and conventional snowflake fin designs, the novel snowflake fin design reduced the total melting and solidification time by 45.15% and 18.14%, respectively. The optimal size of the branch fins is a radial length of the root of 22.9 mm, self-length of 8 mm, and an angle of 60.3°.

Optimizing the Production of Antioxidant and Antibacterial Alkaloids from Macleaya cordata by Subcritical Water Extraction Technology.

In this work, the subcritical water extraction technology was used to extract alkaloids from Macleaya cordata, and the effects of extraction temperature and time on its yield were investigated to find the best extraction conditions. Moreover, the antioxidant capacity and antibacterial activity of Macleaya cordata extract were studied. Furthermore, through the single-factor method, it was found that properly increasing the extraction temperature and prolonging the extraction time was conducive to increasing alkaloid yield. Still, a considerable amount of alkaloids might be decomposed by heat, resulting in a decrease in their yield. The results showed that the optimal extraction temperature of alkaloids from Macleaya cordata with subcritical water is 190 °C, the time is 45 min, and the corresponding maximum yield is 35.19±0.12 mg/g (sanguinarine equivalent in raw materials). In addition, the antioxidation and bacteriostasis abilities of subcritical water extract are better than those of traditional hot water extract, indicating that it is a feasible method to extract alkaloids from Macleaya cordata with subcritical water.

Bifunctional acid-activated montmorillonite catalyzed biodiesel production from non-food oil: Characterization, optimization, kinetic and thermodynamic studies

Utilizing robust heterogeneous catalysts to efficiently produce biodiesel from low-cost non-food oil is very important for replacing fossil energy to meet globally increasing energy demands and accelerate realization of carbon neutrality. In this study, a novel Lewis (L)-Brønsted (B) bifunctional catalytic material 30%Sn-MMT-SO3H was conveniently fabricated by ion-exchange and sulfonation using widely-sourced and biocompatible montmorillonite (MMT) as a matrix, which was successfully applied in direct conversion of Jatropha curcas oil (JCO) into biodiesel. Systematical characterization techniques showed that 30%Sn-MMT-SO3H presented a layered nanostructure along with a high specific surface area (145.32 m2/g), rich mesopores (5.8 nm), strong acidic intensity (1.801 mmol g−1), abundant accessible active sites, and outstanding chemical and thermal stability. Additionally, the single-factor method and response surface methodology (RSM) were employed to investigate optimal reaction conditions. Accordingly, the maximum biodiesel yield (93.1%) was achieved under conditions of 7 wt% catalyst dosage, 20.2 methanol/oil molar ratio at 150 °C for 7.5 h, and no significant decrease was observed after five cycles. Importantly, kinetic and thermodynamic behaviors together with possible reaction mechanisms were also determined. Compared with other reported catalysts, 30%Sn-MMT-SO3H exhibited satisfactory activity and reusability, showing promising prospects in industrialization of biodiesel mediated by heterogeneous acid catalysts.

Structural characteristics and biological activity of lactic acid bacteria exopolysaccharides separated by ethanol/(NH4)2SO4 ATPS

Exopolysaccharides (EPS) from lactic acid bacteria (LAB) as edible and safe bioproducts with health benefits have become an interesting topic. In this study, aqueous two-phase system (ATPS) was established using ethanol and (NH4)2SO4 as phase-forming substances to separate and purify LAB EPS from Lactobacillus plantarum 1.0665. The operating conditions were optimized by a single factor and response surface method (RSM). The results indicated that an effectively selective separation of LAB EPS was achieved by the ATPS consisted of 28 % (w/w) ethanol and 18 % (w/w) (NH4)2SO4 at pH 4.0. Under optimized conditions, the partition coefficient (K) and recovery rate (Y) were well matched with the predicted value of 3.83 ± 0.019 and 74.66 ± 1.05 %. The physicochemical properties of purified LAB EPS were characterized by various technologies. According to the results, LAB EPS was a complex polysaccharide with a triple helix structure mainly composed of mannose, glucose and galactose in the molar ratio of 1.00: 0.32: 0.14, and it proved that the ethanol/(NH4)2SO4 system had good selectivity for LAB EPS. In addition, LAB EPS displayed excellent antioxidant activity, antihypertension activity, anti-gout capacity and hypoglycemic activity in vitro analysis. The results suggested that LAB EPS could be a dietary supplement applied in functional foods.

Study of Atmospheric Pressure Plasma Temperature Based on Silicon Carbide Etching.

In order to further understand the excitation process of inductively coupled plasma (ICP) and improve the etching efficiency of silicon carbide (SiC), the effect of temperature and atmospheric pressure on plasma etching of silicon carbide was investigated. Based on the infrared temperature measurement method, the temperature of the plasma reaction region was measured. The single factor method was used to study the effect of the working gas flow rate and the RF power on the plasma region temperature. Fixed-point processing of SiC wafers analyzes the effect of plasma region temperature on the etching rate. The experimental results showed that the plasma temperature increased with increasing Ar gas until it reached the maximum value at 15 slm and decreased with increasing flow rate; the plasma temperature increased with a CF4 flow rate from 0 to 45 sccm until the temperature stabilized when the flow rate reached 45 sccm. The higher the RF power, the higher the plasma region's temperature. The higher the plasma region temperature, the faster the etching rate and the more pronounced the effect on the non-linear effect of the removal function. Therefore, it can be determined that for ICP processing-based chemical reactions, the increase in plasma reaction region temperature leads to a faster SiC etching rate. By processing the dwell time in sections, the nonlinear effect caused by the heat accumulation on the component surface is effectively improved.

Permeability prediction based on dual seismic pore structure factors

Pore structure, especially pore shape and size, is a key factor affecting reservoir permeability. There is a poor correlation between permeability and porosity when the pore structure is complex, which leads to the low accuracy of permeability prediction by using porosity. In view of the preceding problems, six factors related to pore structure are summarized from rock physical models and entitled seismic pore structure factors (SPSFs), and the sensitivity of SPSF to permeability is analyzed with the data of two groups of clastic rock cores. The results find that the shear Lee factor is most sensitive to permeability, followed by the shear flexibility factor. In addition, there are good linear correlations between the shear Lee factor and pore size and between the shear flexibility factor and pore aspect ratio. Therefore, the combination of the shear Lee factor and pore aspect ratio can better characterize pore structure, and thus reservoir permeability. A seismic permeability prediction method based on pore aspect ratio, the shear Lee factor, and lithofacies is developed and applied to the Jurassic tight sandstone reservoirs of the Sichuan Basin. The results find that the permeability prediction accuracy is within half an order of magnitude, which is five times higher than the single-factor method of the shear flexibility factor. Compared with porosity, permeability distribution is more consistent with the measured productivity, which confirms the effectiveness of the method. It also provides a new way to predict the spatial distribution of reservoir permeability.

Temperature-Sensitive Modified Bentonite Based on NIPAM for Drilling Fluid: Experimental and Molecular Simulation Studies.

Bentonite is an important component of drilling fluid, whose quality directly affects the safety and economic benefits of water-based drilling fluid. In order to effectively cope with temperature changes, the development of temperature-sensitive modified bentonite is of great significance. In this study, a temperature-sensitive modified bentonite based on NIPAM with excellent temperature sensitivity was developed through intercalation modification. The temperature-sensitive bentonite (CMC-B-NIPAM) was prepared by grafting N-isopropyl acrylamide (NIPAM) onto the surface of calcium bentonite through the dehydration condensation of silane coupling agent KH570 after the intercalation of sodium Carboxymethyl Cellulose (CMC). The synthesis indexes of CMC-B and CMC-B-NIPAM were optimized by the single-factor method. CMC-B-NIPAM was characterized by XRD and FTIR. The temperature sensitivity, rheology, suspensibility, and expansion capacity of CMC-B-NIPAM dispersion were investigated. The results showed that CMC-B-NIPAM had good temperature sensitivity, and the rheological properties of its dispersion showed characteristics of steady flow and temperature thickening in the range of 40-70 °C. A molecular simulation model was established to observe the microsynthesis mechanism of temperature-sensitive modified bentonite based on NIPAM. The results of this study show that CMC-B-NIPAM drilling fluid has the function of ensuring the stability of drilling fluid flow patterns compared to traditional drilling fluids.

RESPONSE SURFACE OPTIMIZATION BASED ON FREEZE-THAW CYCLE PRETREATMENT OF POPLAR WOOD DYEING EFFECT

To improve the permeability of dye solution in wood, poplar was pretreated by freeze-thaw cycle. The effects of three parameters, such as freezing time, thawing time and circulation times on the dyeing effect of pretreated poplar were investigated by single factor method. On this basis, pretreatment conditions were optimized by response surface methodology. The optimum conditions of this treatment were freezing time of 11.9 h, thawing time of 7.2 h and two cycles. Under these conditions, the dye uptake and chromatic aberration of the treated wood were 25.77% and 21.14%, respectively, which were close to the theoretical predicted values. The results showed that freeze-thaw cycle pretreatment could effectively improve the permeability of poplar and enhance the dyeing effect of wood.

Experimental Study on Rheological Properties of Coal Gangue Slurry Based on Response Surface Methodology

To handle the gangue well and control the settlement of the surface, as well as to reduce the risk of water bleeding, settlement and even blockage and pipe breaking of the gangue slurry in the process of conveying, the rheological characteristics of the slurry should be studied. The rheological properties of slurry with different concentrations prepared from gangue samples of the Ningtiaota coal mine were tested, and the correlation between the rheological characteristics of the coal gangue filling slurry and three factors, namely the gangue mass fraction, grain gradation and standing time, were studied by a single factor method and response surface methodology. The results show that the fitting curve of the Herschel–Bulkley model is mostly linear, that is, the shear stress of coal gangue paste increases as a function of the shear rate. Therefore, these two concentrations are too small to form a stable network structure to wrap large particles and can easily cause pipe blockage. The yield shear stress and plastic viscosity show an exponential increase with the increasing mass fraction. The shear stress and apparent viscosity of the pastes with mass fractions of 60% and 65%, respectively, increase significantly after 20, 40 and 60 min of standing. According to the comprehensive test results and the response surface, the optimization method is as follows: mass fraction of 72%; aggregate grading for 4.75~1.18 mm particle size is 30%, for 1.18~0.425 mm particle size is 40%, for 0.425~0.075 mm particle size is 10%, for less than 0.075 mm particle size is 20%; with different standing times, the yield shear stress of slurry ranges from 103.02 to 131.645 Pa; and the plastic viscosity ranges from 0.54 to 0.64 Pa.s. With the increase of the standing time, the slurry settlement is relatively small, and is a more ideal gangue slurry proportion.

Effect of cavity-confinement and microwave-assistance on the sensitivity of LIBS for detecting Cu content in pig feed

The laser-induced breakdown spectroscopy (LIBS) is a potential tool for detecting heavy metals in pig feed. In order to implement a highly sensitive LIBS system required for the detection of Copper in pig feed and achieve better detection results, this work combines cavity-confinement and microwave-assistance with the LIBS system. Based on the characteristic spectral intensity of Cu I 324.75 nm, four factors including delay time, laser energy, cavity-confinement diameter, and microwave-assisted power are optimized by using single factor method and response surface methodology (RSM) for obtaining suitable experimental parameters and detection performance. The detection results show that as compared with the single factor method, the optimal experimental parameters obtained by using RSM increase the intensity of the characteristic spectral lines by 21.69% and the detection limit is as low as 0.878 mg/kg. As compared with the traditional LIBS techniques, the use of cavity-confinement combined with microwave-assistance increase the detection sensitivity of LIBS technique by 18.6-fold. The results show that RSM effectively analyzes the interactions between factors and their effects on the response values to effectively obtain better values for each factor. This enhances the LIBS spectra by cavity-confinement and microwave-assistance, and improves the sensitivity of detection of Cu in the pig feeds, which is suitable for realizing the quantitative detection of low concentration elements in pig feeds.

A fiber-packed needle-type extraction device with ionic liquid-based molecularly imprinted polymer as coating for extraction of chlorobenzenes in water samples

A needle-type extraction device (NTED) packed with ionic liquid-based molecularly imprinted polymers (MIP) coated copper fiber was produced for extraction of chlorobenzenes (CBs) in the aqueous environment followed by detection with GC-FID. Precipitation polymerization was performed to synthesize CBs imprinted polymers (CBs-MIP) with CBs as a mixed template, and 1-vinyl-3-aminoformylmethyl imidazolium chloride ionic liquid ([VAFMIM]Cl) as a functional monomer. Then CBs-MIP was coated on the braided copper fiber surface by sol–gel technology, which was served as a coated fiber to extract five trace CBs in drinking water, river water and industrial wastewater. The effects of several experimental conditions on extraction, including extraction time and temperature, pump flow rate, stirring speed, desorption time and temperature were studied by the single factor and experimental design method. A Plackett-Burman design (PBD) was used to screen the significant factors and a Box-Behnken design (BBD) was applied to optimize the significant variables to obtain the optimum extraction conditions. The limits of detection (0.0073–0.0130 μg L−1) and enrichment factors (2158–4087) for studied CBs were obtained after optimization. Moreover, the recoveries of CBs spiked in real water samples were 80.01–97.10% with RSD less than 7.70%. The experimental results confirmed that the NTED packed with CBs-MIP coated fiber is a promising device for extracting trace CBs from the water matrix.

3,4-Enhanced Polymerization of Isoprene Catalyzed by Side-Arm Tridentate Iminopyridine Iron Complex with High Activity: Optimization via Response Surface Methodology.

3,4-Enhanced polymerization of isoprene catalyzed by late transition metal with high activity remains one of the great challenges in synthetic rubber chemistry. Herein, a library of [N, N, X] tridentate iminopyridine iron chloride pre-catalysts (Fe 1-4) with the side arm were synthesized and confirmed by the element analysis and HRMS. All the iron compounds served as highly efficient pre-catalysts for 3,4-enhanced (up to 62%) isoprene polymerization when 500 equivalent MAOs were utilized as co-catalysts, delivering the corresponding high-performance polyisoprenes. Furthermore, optimization via single factor and response surface method, it was observed that the highest activity was obtained by complex Fe 2 with 4.0889 × 107 g·mol(Fe)-1·h-1 under the following conditions: Al/Fe = 683; IP/Fe = 7095; t = 0.52 min.

Evaluation of compressive strength of cement-stabilized rammed earth wall by ultrasonic-rebound combined method

It is difficult to guarantee the construction quality of rammed earth buildings, and there needs to be more guidance for the quality inspection of historical protection buildings. To facilitate the assessment of the quality of rammed earth walls, based on the establishment of concrete strength measurement curves, this study took the additive content and type with curing time as variables, and considered the rebound (R) values and ultrasonic pulse velocity (UPV) values in the dense and sparse zones. Based on the material strength theory, the strength measurement curve of cement-stabilized rammed earth was finally established in the sparse zone. The single-factor method and two-factor method were considered to evaluate the unconfined compressive strength (UCS), respectively. The results showed that the R value and UPV increased with the increase in cement content. With the increase in curing time, the R value increased and stabilized; the UPV increased, decreased, and finally stabilized. The difference in the R value of the sparse and dense zones is within ±1, and the difference in the UPV is within ±0.2 km/s. The single R method or UPV method predicts the UCS with poor accuracy, while the ultrasonic-rebound combined method can better predict the UCS. Finally, samples from the failed specimens were taken for scanning electron microscopy tests to further verify the test results on a microscopic level.

Experimental study on electrostatic atomization effect of capillary-plate electrode

The electrostatic atomization effect of liquid in high voltage electrostatic field was studied under capillary-flat electrodes. The effects of charging voltage, liquid flow rate, and the distance between a capillary and flat electrode on droplet size distribution were analyzed by the DP-02 laser particle size analyzer. The single-factor method was used to analyze the effects. The experimental results show that the charging voltage, the liquid flow rate, and the distance between the capillary and the plate electrode greatly influence the droplet's atomization effect. The median particle size D50 and the distribution span (Span) decrease with the increase of charging voltage, the reduction of liquid flow and the decline of the distance between capillary and plate electrode. That is, the droplet particle size distribution is better, and thus the droplet electrostatic atomization effect is better.

Antioxidant capacity and antitumor activity of the bioactive protein prepared from orange peel residues as a by-product using fungal protease

Agricultural and industrial residues (AIR) are renewable biomass sources present in large quantities causing pollution. Converting AIR to eco-friendly products (bioactive materials) reduces their quantity and impact on the environment, in addition to reducing production costs. Therefore, orange peel (OP) protein degradation, antioxidant capacity, and antitumor activity were investigated using Aspergillus niger WA 2017 protease. The highest value of the protein hydrolysate with the highest antioxidant using the DPPH method was obtained after 24 h. The single-factor method boosted the protein hydrolysate and the DPPH antioxidant activity by 3.7 and 1.7-fold, respectively. Statistical optimized conditions (Central Composite Method) increased the hydrolysate value and the DPPH antioxidant activity by 1.6 and 1.1-fold, respectively. The central trial samples exhibited the highest DPPH antioxidant activity (62.37 %), while the control sample recorded 20 %. All antioxidant tests in vitro (DPPH, reducing power, ABTS, and FRAP) confirmed the superiority of the potent hydrolysate as a good antioxidant. In vitro antitumor activity, the potent hydrolysate exhibited the highest effect on the Ehrlich Ascites Carcinoma Cells viability as it recorded 60.62 % dead cells. In vivo antitumor activity, the volume of the untreated tumor mice was found to be 1.4-fold bigger than the volume obtained from the potent hydrolysate. The increase in life span (ILS %) for oral treatment and intraperitoneal injection treatment with the potent hydrolysate increased by 13.91 and 19.42 %, respectively, compared to the untreated tumor.

Optimization of Submerged Culture Parameters of the Aphid Pathogenic Fungus Fusarium equiseti Based on Sporulation and Mycelial Biomass.

Fusarium equiseti (JMF-01), as an entomopathogenic fungus, can effectively control agricultural pests and has the potential to be a biocontrol agent. To promote mycelial growth and sporulation, we investigated the optimal submerged culture conditions for F. equiseti. In this study, we used the single-factor method and Box-Behnken design and determined the virulence of the submerged culture against Myzus persicae after optimization. As a result, the highly significant factors affecting the spore concentration of strain JMF-01 were the primary inoculum density and the initial pH, and the highly significant factor affecting the mycelial biomass was the medium-to-flask ratio. The highest mycelial biomass value was 0.35 g when the incubation time was 5.68 days, the initial pH was 5.11, the medium-to-flask ratio was 0.43, and 1 mL of the primary inoculum with spore density of 0.97 × 107 conidia/mL was added. When the incubation time was 6.32 days, the initial pH was 4.46, the medium-to-flask ratio was 0.35, the primary inoculum density was 1.32 × 107 conidia/mL of 1 mL, and the highest spore concentration of 6.49 × 108 blastospores/mL was obtained. Compared with the unoptimized medium conditions, the optimized submerged culture had the highest mycelial biomass and spore concentration, which were 3.46 and 2.06 times higher, respectively. The optimized submerged culture was highly pathogenic toward M. persicae, reaching a 95% mortality rate. Our results provide optimal submerged culture conditions for F. equiseti and lay the basis for later research to expand production for pest control.

The Study on Kaolin/corn Stalk Composite Adsorbent Materials Prepared by H3PO4 Activation Method

Using single factor method and orthogonal experiment,the study takes Kaolin and corn straw as raw materials and H3PO4 as activator and dips them in different activator concentration,different kaolin and corn straw mass ratio as well as different impregnation ratio under different conditions, and then carbonize them in low temperature by electric furnace, muff calcining to prepare kaolin/corn straw composite adsorption material.The adsorption capacity for methylene blue solution was characterized by T6 visible spectrophotometer,and automatic specific surface area and porosity analyzer was used to characterize the composite adsorption material under the optimal experimental conditions. The results show that the optimal experimental conditions are: Under the conditions of 25% Concentration of H3PO4 , 1:4 mass ratio of kaolin to corn stalk and 2.5:1 impregnation ratio, the adsorption value of methylene blue on the composite adsorbent is 1.116mg/g, the specific surface area is 7.56m2·g-1 and the single specific surface area is 6.82m2·g-1. The microporous volume was 1.24×10-8mL·g-1 and the microporous area was 0.57m2·g-1，the results show that the pore structure of the composite adsorbent is not uniform, and there are a lot of micropores and a certain amount of mesopores in the samples.

Phenolic Compounds of Fig (Ficus carica L.) Leaves: Optimization of Extraction by Response Surface Methodology and UPLC-MS Phytochemical Characterization

Background: This study is based on ethnobotanical observation and traditional medicinal utilization of fig (Ficus carica L.) by southwest Asian and Mediterranean populations. Objective: Considering phenolics among the highly bioactive compounds for their numerous therapeutic applications, the first goal of this investigation is the optimization of their recovery by using Response Surface Methodology (RSM). Methods: Prior to RSM, the single-factor method was applied for revealing the main factors influencing the extraction (solvent type, solvent concentration, sample˗to˗solvent ratio, temperature, and time of extraction). After using the optimal conditions of extraction, the second objective was the identification of fig leave phytochemicals by UPLC˗MS. Results: The results of preliminary optimization step through sequential methodology revealed that solvent concentration, extraction duration, and temperature, and were the main influencing factors. All these tested extraction parameters influenced significantly total phenolic contents (TPC) and DPPH-free radical scavenging activity (FRSA). RSM analysis using Box˗Behnken design showed that the optimal extraction conditions of total phenolic contents of fig leaves were 23.07% acetone, 51.82 °C, and 118.02 min with values of 29.44 mg GAE/g and 59.48% for TPC and FRSA, respectively. Eight phenolic compounds were detected including chlorogenic, caffeic, and coumaric acids, rutin, isoquercetin, luteolin, quercetin, and kaempferol well known for their bioactivities. Two non˗phenolic compounds were also found: The psoralen and an oxylipin, the 9˗oxo˗octadecadienoic acid that was detected for the first time in fig leaves. Conclusion: Through sequential and RSM optimization processes for phenolic antioxidants of Ficus carica leaves, the developed mathematic models have expressed a high level of significance. The UPLC-MS analysis of the optimal extract revealed numerous phenolic compounds known for their bioactivities.