PurposeTo assess the ability of dual-energy CT (DECT) as a novel technique to quantify the degree of rotator cuff fat degeneration. MethodClinically indicated shoulder CT exams for evaluation of osteoarthritis, rotator cuff arthropathy, pain or instability, or preoperative planning were acquired using dual-source CT systems. Rotator cuff DECT fat fraction after material decomposition was calculated off the sagittal image. Fat fractions were also assessed using CT numbers from dual energy virtual monochromatic images (70 keV) and single-energy CT (SECT) images (100 kV). Visual subjective Goutallier scores of the rotator cuff muscles were used as the reference standard. Results12 shoulders from 10 patients were analyzed, with bilateral shoulders evaluated in two patients (mean age 69 years (range 19–97)). Three patients were male and seven were female, with mean BMI of 32 (range 26–41). Mean fat fraction of the teres major and subcutaneous fat were, 2.9 % ± 4.0 % and 99.5 % ± 2.6 %, respectively, rendering these as reliable internal standards for 0% and 100 % fat. Mean DECT fat fractions of the rotator cuff were compared to Goutallier scores, revealing a high strength of rank correlation: ρ = 0.92, p < 0.0001. Mean fat fraction assessed with CT numbers also revealed high strengths of linear associations: ρ = 0.83, p < 0.0001 and ρ = 0.82, p < 0.0001, for DECT 70 keV and SECT 100 kV, respectively. ConclusionsDECT direct fat fraction after material decomposition presents a novel approach to quantitative assessment of fatty degeneration, which has excellent correlation with clinically accepted standards.