The usage of functionalized conductive polymer has been a promising choice for flexible triboelectric nanogenerators (TENGs). However, how to integrate the solid and rigid film into a stretchable TENG for inspecting human motion or gesture still pose challenges. In this work, a transparent and stretchable wrinkled PEDOT:PSS electrode based TENG (WP-TENG) has been fabricated. The inner wrinkle distance and thickness are ~5 μm and ~1.81 μm, respectively, with maximum stretched strain of ~100%. As an electrode, the optimum conductivity and transparency can reach 0.14 kΩ/□ and 90%, respectively. Operating in the single-electrode mode at 2.5 Hz, the WP-TENG with an area of 6 × 3 cm2 produces an open-circuit voltage of 180 V and short-circuit current of 22.6 μA. It can be worn on the wrist to harvest hand tapping energy and charge a commercial capacitor to 2 V in ~ 3.5 min, and then drive an electronic watch for more than 30 s. Furthermore, by attaching to human skin, the WP-TENG can be treated as an active human motion monitoring sensor, which could inspect the bending angle of the elbow and joint of human by analyzing the peak value of voltage output and monitor the motion frequency by counting the peak number. The triboelectric mechanism also enables the WP-TENG to realize high-performance active tactile sensing. The active sensor with an external resistor of 100 MΩ illustrates the sensitivity of 0.08 kPa−1 in the pressure range from 2 to 60 kPa, and 0.008 k Pa−1 from 60 to 160 kPa, respectively. The assembled 3 pixel × 3 pixel tactile sensor array was fabricated for mapping the touch location or recording the shape of object contacted with the sensor array. The remarkable active sensing capability of WP-TENG with superior transparency and stretchability exhibits the development prospect in novel applications, such as human-machine interaction, e-skins, soft robots and wearable electronics, etc. Figure 1