Single Df Research Articles

Carcass fabrication yields of beef steers supplemented zilpaterol hydrochloride and offered ad libitum or maintenance energy intake.

An experiment was conducted to evaluate the fabrication yields of carcasses from beef steers supplemented zilpaterol hydrochloride (ZH) and fed at maintenance (MA) or ad libitum (AB) intake levels. Beef steers (n = 56) from a common sire were blocked (n = 28 per block) by terminal growth implant and sorted into pairs by BW. Four pairs (n = 8) were harvested on day 0; the remaining 24 pairs (n = 48) were assigned to a dietary intake level (MA or AB) and days on feed (28 or 56 d). Within pairs of MA or AB intakes, steers harvested on day 56 were randomly assigned to supplementation of ZH (90 mg·d−1 per steer) for 20 d followed by a withdrawal period of 4 d or control (C). Steers (BW = 603.5 ± 48.1 kg) were harvested at a commercial processing facility. After a 24-h chill period, standard USDA grading procedures were used to derive a calculated yield grade and quality grade. Following grading, left carcass sides were transported to the West Texas A&M University Meat Laboratory for fabrication. Each side was fabricated into subprimals to determine individual red meat yield (RMY), trimmable fat yield (TFY), and bone yield (BY). A mixed model was used for analysis; fixed effects included treatment combinations and random effects included block and pairs. Single df contrasts tested day 0 vs. 28, day 0 vs. 56, day 28 vs. 56, MA vs. AB, and C vs. ZH. Yield of chuck eye roll differed (P = 0.05) by days on feed (0 d = 4.14, 28 d = 4.11, 56 d = 4.55%). Similarly, eye of round yield was impacted (P = 0.02) by days on feed (0 d = 1.51, 28 d = 1.37, 56 d = 1.36%). Additionally, brisket yield was altered (P < 0.01) by days on feed (0 d = 4.08, 28 d = 3.56, 56 d = 3.48%) and treatment (C = 3.34, ZH = 3.61%). For remaining subprimals, no differences (P ≥ 0.15) were detected. Furthermore, results indicated that RMY tended (P = 0.07) to differ by treatment (C = 61.35, ZH = 63.67%). Comparatively, TFY was impacted (P = 0.04) by intake (MA = 20.44, AB = 23.33%). Results from this study indicate that a MA intake level during the last 56 d of the finishing period concurrent with ZH supplementation impacts subprimal yields as well as carcass RMY and TFY of beef steers.

Live growth performance, carcass grading characteristics, and harvest yields of beef steers supplemented zilpaterol hydrochloride and offered ad libitum or maintenance energy intake.

A trial was conducted to examine live growth efficiency, harvest yields, and carcass grading performance of steers fed at maintenance (M) or at ad libitum (A) level of intake during zilpaterol hydrochloride (Z) supplementation. Single-sired, beef steers (n = 56; start of trial BW 590 ± 36 kg) blocked (n = 2) by weight and terminal implant were sorted into pairs (n = 14 per block) by weight. Pairs of steers were initially assigned to 0, 28, or 56 d of feeding. Within 28 or 56 d, pairs were assigned to M or A intake. Steers within a pair assigned to 56 d of feeding were randomly assigned to either 20 d of Z supplementation (90 mg/d per steer) with a 4 d withdrawal period prior to slaughter or to no ZH supplementation (C). Steers were housed and fed in individual pens. Weights of all non-carcass and carcass components were recorded at slaughter; carcasses were graded 24-h postmortem. Data were analyzed via a mixed model; the fixed effect was treatment combination with random effects of block and pair. Live growth data used harvest day as the repeated measure and animal as the subject. Single df contrasts were constructed for day 0 vs. day 28, day 0 vs. day 56, day 28 vs. day 56, M vs. A, and C vs. Z. Treatment impacted (P ≤ 0.05) live ADG; contrasts indicated A (1.33) was greater than M (0.14 kg), and Z (1.12) was greater than C (0.82 kg). Similarly, carcass ADG differences (P < 0.01) indicated A (1.04) was greater than M (0.36 kg), and Z (1.35) was greater than C (0.71 kg). Intake level altered BW and empty body weight (EBW); M cattle had reduced BW and EBW (P < 0.01, 585 and 540 kg) than A cattle (647 and 597 kg). Cattle fed at M had less carcass and internal cavity mass (P < 0.01, 359 and 79.4 kg) than A cattle (394 and 93.5 kg). Liver mass was reduced by M feeding (P < 0.01; M-5.03, A-6.69 kg) and Z treatment (P < 0.01; Z-5.64, C-6.06 kg). Moreover, mass of total splanchnic tissue was less (P < 0.01) for M cattle than A cattle (59.8 vs. 72.5 kg). Dressed carcass yield was greater (P < 0.01) for Z than C cattle (63.5 vs. 61.6%). Cattle fed at M had less 12th rib s.c. fat, lower numerical U.S. yield grades (P < 0.01; M-1.71 cm and 3.3, A-2.46 cm and 4.3) and lower numerical Canadian yield grades (P < 0.01; 51.9 vs. 53.9% for M and A, respectively) than A cattle. Results indicate that energy intake level and Z supplementation influence live and carcass growth, carcass transfer, kill yields, and carcass characteristics across time.

μ-Calpain (CAPN1), calpastatin (CAST), and growth hormone receptor (GHR) genetic effects on Angus beef heifer performance traits and reproduction

Genetic marker effects and type of inheritance are estimated with poor precision when minor marker allele frequencies are low. An Angus population was subjected to marker assisted selection for multiple years to equalize CAPN1 haplotypes, CAST, and GHR genetic marker frequencies. The objective was to estimate the pleiotropic effects of these carcass quality oriented markers for body weight, reproduction, and first calf performance traits in 174 replacement beef females which were managed under 2 post-weaning development protocols. Heifers were weighed at 11-, 12-, and 13-mo, at first breeding season pregnancy evaluation, and prior to first calving season. Pubertal status was determined at 11-, 12-, and 13-mo of age. Antral follicles were counted, reproductive tracts were scored, and tract dimensions were measured at 13-mo. Body condition and hip height were scored and measured at pregnancy evaluation and prior to calving season. Heifer pregnancy and weaning rates and ordinal birth date were recorded. Calf body weights at birth and weaning were analyzed. Single df linear contrasts for recessive effects of the GHR heterozygous genotype showed significant decreases of 2.5–3.6% in 11-, 12-, and 13-mo heifer body weights and heifer weight prior to calving. The additive differences between GHR homozygotes were small and not significant for all body weights measured but a 1 wk difference in calf birth date was significant. For all 13-mo uterine measurements, scores, and antral follicle counts, only the CAST dominance contrast for medium antral follicle count was significant. The CAPN1 haplotype with a strong additive effect for increased beef tenderness also had a significant additive effect on calving date. Heifers homozygous for the tender haplotype calved 7.9 days later than heifers homozygous for the tough haplotype. Most heifer reproductive traits were not significantly affected by CAST and CAPN1 markers that are widely used to improve beef tenderness by selection and breeders should not be concerned with how these markers affect reproduction and other heifer traits with the possible exception of CAPN1 effects on calving date.

Improved PLL-based MUSIC Algorithm for Single Receiver DF Systems

In this paper we propose an improved version of a single receiver DF system that combines a PLL scheme with the MUSIC algorithm to obtain both azimuthal and zenithal angles. The new approach uses a more efficient algorithm to remove the typical ambiguities of PLL-based schemes. We carry out several experiments to assess the algorithm performance. Among them, we consider complex environments, such as wireless communication channels where the received signal is corrupted by noise and suffers from distortion, interference, and multipath effects. The new scheme is tested for BPSK and QPSK signals under these conditions. The simulated results show  superior performance of the proposed PLL algorithm when compared to previous techniques.

Performance of amplify forward and decode forward cooperative strategies for body surface communications in UWB Body Area Networks

SummaryBody area networks (BAN) are being developed for telemonitoring of patients. Ultra wideband (UWB) is the most preferred choice for the physical layer of the BAN as it provides high data rate at low power consumption along with a simple transceiver structure. But because of high fading characteristics of the human body channel, the range of UWB will be limited, and a reliable communication cannot be guaranteed. In that case, cooperative communications may be applied to BAN for improving system performance and link quality.The paper proposes a novel approach by applying amplify and forward, and decode and forward cooperative relaying strategies with maximal ratio combining for BAN. The performance of single relay AF and DF relaying have been compared with direct transmission. Further, the efficacy of the proposed strategies has been validated by transmitting electrocardiogram signals and magnetic resonance imaging images. Simulations and validations have been carried out on Institute of Electrical and Electronics Engineers designated CM3 channel model for BAN designed from real time measurements data. A signal‐to‐noise ratio gain of at least 3 dB is clearly observed from simulation results. Thus, transmit power requirement can be minimized, which in turn improves the battery life of body sensor nodes. Copyright © 2015 John Wiley &amp; Sons, Ltd.

Effects of feeding ractopamine (Paylean) on growth and carcass traits in finishing pigs marketed at equal slaughter weights

The objective of this study was to determine the effect of feeding ractopamine hydrochloride (RAC) in an equal weight marketing scenario. Barrows and gilts (n = 427) were assigned to 3 different RAC feeding programs before slaughter: control (0 mg/kg), 5 mg/kg for up to 35 d, or 5 mg/kg for 21 d followed by a step-up to 7.4 mg/kg for up to 14 d. Treatments including RAC were pooled and compared against control pigs using single df estimate statements. Coefficient of variation in beginning BW of RAC (7.16%) and control pigs (6.95%) did not (P = 0.68) differ; however, RAC-fed pigs had a lower (P < 0.01) market BW CV (3.91%) than controls (5.27%). Pigs fed RAC reached market weight 2.5 d earlier (P = 0.01) than control pigs (26.8 vs. 29.3 d). Ractopamine-fed pigs had a 21% greater (P < 0.01) ADG than control pigs, even though ADFI did not (P = 0.19) differ between RAC and control pigs. Pigs fed RAC produced 3% heavier HCW (P < 0.01), 3% greater loin depths (P = 0.05), and a 1 percentage unit greater dressing percent (P < 0.01) than control pigs. Sort loss per kilogram was less (P < 0.01) for RAC-fed than control-fed pigs, with 8 control pigs having HCW less than the sort loss threshold compared with only 2 RAC pigs. Ractopamine can be an important tool in reducing the variation in market weight and sort loss of finishing pigs.

Combining an Internal Pilot with an Interim Analysis for Single Degree of Freedom Tests

An internal pilot with interim analysis (IPIA) design combines interim power analysis (an internal pilot) with interim data analysis (two-stage group sequential). We provide IPIA methods for single df hypotheses within the Gaussian general linear model, including one and two group t tests. The design allows early stopping for efficacy and futility while also re-estimating sample size based on an interim variance estimate. Study planning in small samples requires the exact and computable forms reported here. The formulation gives fast and accurate calculations of power, Type I error rate, and expected sample size.

Evaluation of the Quantitative Gated SPECT (QGS) software program in the presence of large perfusion defects

To evaluate the reproducibility and operator dependence for the quantitative regional left ventricular functional parameters (LVFP) assessed by Cedars-Sinai's Quantitative automated gated SPECT (QGS) software. The QGS algorithm was reviewed in detail and potential operator dependencies were defined. Series of prototypes were selected, consisting of (a) normal perfusion, (b) perfusion defects in all perfusion regions, (c) perfusion studies of patients with angiographic confirmed normal coronary arteries, proximal (>or=70% stenoses) single and multiple vessel disease, and (d) spurious activity in close proximity. While defining and re-orienting the volume containing the left ventricle, the operator adjusted 8 variables/degrees of freedom (DF). The software was used without further operator interventions. Results were expressed as a coefficient of variation (COV). Separate COV were calculated per distinct DF. A segment was considered not robust when the COV did exceed 20% in a single DF, 15% in at least 2 DF, or 10% in at least 3 DF. Regional left ventricular EF and volumes showed excellent reproducibility. Normal perfusion and the vessel disease prototypes showed an excellent COV (for all re-orientation steps [33/prototype]) mostly below 5% for LVFP. However, regional wall motion and thickening became less reliable in the presence of large perfusion defects or artifacts. Quantitative estimates for regional left ventricular functional data show excellent reproducibility using automated gated SPECT. However, there may be substantial operator dependency in the presence of large defects or spurious activity in close proximity.

Neuromuscular Adaptation During Skill Acquisition on a Two Degree-of-Freedom Target-Acquisition Task: Dynamic Movement

In this experiment, we examined the extent to which the spatiotemporal reorganization of muscle synergies mediates skill acquisition on a two degree-of-freedom (df) target-acquisition task. Eight participants completed five practice sessions on consecutive days. During each session they practiced movements to eight target positions presented by a visual display. The movements required combinations of flexion/extension and pronation/supination of the elbow joint complex. During practice sessions, eight targets displaced 5.4 cm from the start position (representing joint excursions of 54 degrees) were presented 16 times. During pre- and posttests, participants acquired the targets at two distances (3.6 cm [36 degrees] and 7.2 cm [72 degrees]). EMG data were recorded from eight muscles contributing to the movements during the pre- and posttests. Most targets were acquired more rapidly after the practice period. Performance improvements were, in most target directions, accompanied by increases in the smoothness of the movement trajectories. When target acquisition required movement in both dfs, there were also practice-related decreases in the extent to which the trajectories deviated from a direct path to the target. The contribution of monofunctional muscles (those producing torque in a single df) increased with practice during movements in which they acted as agonists. The activity in bifunctional muscles (those contributing torque in both dfs) remained at pretest levels in most movements. The results suggest that performance gains were mediated primarily by changes in the spatial organization of muscles synergies. These changes were expressed most prominently in terms of the magnitude of activation of the monofunctional muscles.

Bayesian inference on order-constrained parameters in generalized linear models.

In biomedical studies, there is often interest in assessing the association between one or more ordered categorical predictors and an outcome variable, adjusting for covariates. For a k-level predictor, one typically uses either a k-1 degree of freedom (df) test or a single df trend test, which requires scores for the different levels of the predictor. In the absence of knowledge of a parametric form for the response function, one can incorporate monotonicity constraints to improve the efficiency of tests of association. This article proposes a general Bayesian approach for inference on order-constrained parameters in generalized linear models. Instead of choosing a prior distribution with support on the constrained space, which can result in major computational difficulties, we propose to map draws from an unconstrained posterior density using an isotonic regression transformation. This approach allows flat regions over which increases in the level of a predictor have no effect. Bayes factors for assessing ordered trends can be computed based on the output from a Gibbs sampling algorithm. Results from a simulation study are presented and the approach is applied to data from a time-to-pregnancy study.

The effect of teacher certification and task level on mathematics achievement

In this study it was hypothesized that the degree of content area preparation of seventh grade mathematics teachers would differentially effect student performance as a function of the level of the mathematics tasks used to assess that performance. More specifically, students of high MATHPREP teachers were hypothesized to outperform those of low MATHPREP teachers on the higher level tasks. The sample consisted of over 9000 seventh grade students from 33 matched pairs of schools whose teachers differed on level of mathematics preparation. Aggregate measures of achievement for the students at each school on test items representing three ordered levels of thinking were obtained. The primary hypothesis was addressed using a single df interaction contrast and produced a statistically and practically significant result in the hypothesized direction. Implications for teacher preparation and the hiring practices of school administrators are considered.

Laser isotope separation and the multiphoton decompotions of formaldehyde using a focused DF laser: the effect of single- or multi-line irradiation

Formadehyde was decomposed by a focused, pulsed DF laser. Simultaneous irradiation with multiple DF lines gives yields almost 100 times those obtained with single DF line irradiations of comparable total energy. Selective irradiation of H 2CO in equimolar li 2CO/D 2CO gives large hydrogen isotope enrichment for low pressures.