Single Column Research Articles

Open-source milligram-scale, four channel, automated protein purification system.

Liquid chromatography purification of multiple recombinant proteins, in parallel, could catalyze research and discovery if the processes are fast and approach the robustness of traditional, "one-protein-at-a-time" purification. Here, we report an automated, four channel chromatography platform that we have designed and validated for parallelized protein purification at milligram scales. The device can purify up to four proteins (each with its own single column), has inputs for up to eight buffers or solvents that can be directed to any of the four columns via a network of software-driven valves, and includes an automated fraction collector with ten positions for 1.5 or 5.0 mL collection tubes and four positions for 50 mL collection tubes for each column output. The control software can be accessed either via Python scripting, giving users full access to all steps of the purification process, or via a simple-to-navigate touch screen graphical user interface that does not require knowledge of the command line or any programming language. Using our instrument, we report milligram-scale, parallelized, single-column purification of a panel of mammalian cell expressed coronavirus (SARS-CoV-2, HCoV-229E, HCoV-OC43, HCoV-229E) trimeric Spike and monomeric Receptor Binding Domain (RBD) antigens, and monoclonal antibodies targeting SARS-CoV-2 Spike (S) and Influenza Hemagglutinin (HA). We include a detailed hardware build guide, and have made the controlling software open source, to allow others to build and customize their own protein purifier systems.

Assessment of Bearing Capacity and Settlement Characteristics of Organic Soil Reinforced by Dune Sand and Sodium Silicate Columns: A Numerical Study

Organic soil is problematic soils in geotechnical engineering due to its properties, as it is characterized by high compressibility and low bearing capacity. Therefore, several geotechnical techniques tried to stabilize and improve this soil type. In this study, sodium silicate was used to stabilize sand dune columns. The best sodium silicate concentration (9%) was used, and the stabilized sand dune columns were cured for seven days. The results for this soil were extracted using a numerical analysis program (Plaxis 3D, 2020).In the case of studying the effect of (L/D) (where ‘’L” and ‘’D’’ length and diameter of sand dune columns) of a single column of sand dunes stabilized with sodium silicate with a different diameter, the results showed that the best effect was for L/D of (4) with D equal to (0.7m) in the end bearing columns type. In the case of studying a group of columns, the percentage of improvement in soil bearing ratio increased with increasing the number of columns in both floating and end bearing types. When using (8-columns) with a square distribution pattern, the optimum improvement was at the end bearing and at the ratio L/D of (6), where the improvement percentage was (666%) compared to the unimproved soil.

Modeling and improving the scrubbing efficiency of an intensified ammonia process for coke oven gas purification

Coke oven gas (COG) is a valuable byproduct of coke production in the steel industry. Conventionally, undesired H2S and NH3 in COG are removed in separate columns in an ammonia process. In this work, an intensified ammonia process which removes both H2S and NH3 in a single column with multiple sections is studied. The intensified process occupies less space and is less expensive. A process model is built and validated using plant operating data. The underlying principal of scrubber operation is explained through the simulated concentration profile. The influences of important operating variables such as inlet COG flowrate, strong ammonia liquid flowrate, low ammonia liquid flowrate and concentrated NaOH solution flowrate are studied. It is shown that the H2S removal efficiency is sensitive to inlet COG flowrate, and that the flowrate of low ammonia liquid should be used as manipulate variable to control the outlet COG composition. Furthermore, it is found that to improve the H2S removal efficiency, the flowrate of concentrated NaOH solution should exceed a certain threshold. Otherwise the scrubbing liquid will be neutralized by CO2 in the COG, which will decrease the pH and alkalinity of the scrubbing liquid significantly.

Precise single column resection and reconstruction with femoral head plus total hip replacement for primary malignant peri-acetabulum tumors

To evaluate whether single acetabular column can be reserved and the effect of reconstruction with femoral head plus total hip replacement (THR) for primary malignant peri-acetabulum tumors. From 2007 to 2015, nineteen patients with primary malignant peri-acetabulum tumors were enrolled. All cases underwent single column resection with clear surgical margins. Ten of the 19 tumor’s resections were assisted by computer navigation. Femoral heads were applied to reconstruct anterior or posterior column defects; THR was used for joint reconstruction. The surgical safety, oncologic outcome and prosthesis survivorship and function were evaluated by regular follow-up. The average follow-up period was 65.9 months. Surgical margins contained wide resection in 12 cases and marginal resection in 7 cases. One patient with Ewing's sarcoma died 14 months postoperative due to lung metastasis. One case with chondrosarcoma had recurrence. One prosthesis was removed due to infection. The average MusculoSkeletal Tumor Society (MSTS) function score was 83.7%. Due to the relative small number of cases, there was no significant difference in the recurrence rate and prosthesis failure rate between the navigation group and non-navigation group. Single column resection and reconstruction with femoral head autograft plus THR is an effective, safe method with less complication rate and better functional outcome for patients with peri-acetabular tumors.

Ensuring Structural Integrity: An Evaluation of Vertical Shortening in Tall Concrete Buildings

Vertical shortening, a phenomenon inherent to concrete structures, plays a key role in the quality and safety of construction, particularly in tall reinforced concrete buildings. This behavior is intrinsically linked to the time-dependent properties of concrete, encompassing both creep and shrinkage. Neglecting these aspects, especially when non-uniformly distributed, can give rise to various structural issues, including partition distress, overloading, and potential cracking in horizontal elements. This paper delves into the principal factors influencing vertical shortening and presents a rigorous approach to their evaluation, treating creep and shrinkage as critical parameters. The investigation employs two illustrative case studies: the first revolves around a 15-story reinforced concrete building with single columns supporting tributary areas at various levels, acting as a calibration case; the second encompasses a 30-story reinforced concrete structure employing a dual system, providing a broader perspective applicable to three-dimensional structures. A diverse array of analyses is conducted, incorporating considerations for concrete’s linear and nonlinear behavior as well as the impact of construction stages. Our findings reveal that vertical shortening is directly proportional to the building’s height and inversely related to concrete strength, longitudinal reinforcement ratios, member dimensions, volume-to-surface ratios, age of the structure upon load application, and relative humidity. Consequently, precise assessments of differential shortening effects are paramount. The utilization of staged construction analysis and time-dependent effects is recommended as the most suitable approach for evaluating vertical shortening effects.

Dynamic Self Checking Technology of Transformer Device in Intelligent Power Plant Based on Protection Logic Optimization

So as to ensure the normal operation of transformer device and reduce the heavy loss caused by fault, a dynamic self-test skill of transformer device in smart muscle vegetation under the optimization of protection logic is proposed. The protection logic optimization scheme is proposed, and the single column three winding architecture of the main transformer of the smart muscle vegetation transformer device is constructed. On the basis of this architecture, according to the four arm thermal conductivity pool architecture and the wavelet denoising process, the performance self inspection system of the smart muscle vegetation transformer device is designed. According to the load model of the smart muscle vegetation transformer, Thus, the dynamic self-test skill of smart muscle vegetation transformer device under the protection logic optimization is realized. The laboratorial consequences show that the frequency response curves of the faulty winding and the winding under normal operation are basically the same, which can accurately diagnose the short circuit fault between turns of the transformer, and the self-test skill has strong test sensitivity.

Simultaneous Determination of Transuranium Radionuclides in Urine by Tandem Quadrupole ICP-MS/MS with Mass-Shift Mode Combined with Chemical Separation.

The urine bioassay method for transuranium nuclides (237Np, 239,240,241Pu, 241Am, and 244Cm) is needed to quickly assess the potential internal contamination in emergency situations. However, in the case that the analysis of multiple radionuclides is required in the same sample, time-consuming/tedious sequential analytical procedures using multiple chromatographic separation resins would have to be employed for the separation of every single radionuclide. In this work, a rapid method for the simultaneous determination of transuranium nuclides in urine was developed by using triple quadrupole inductively coupled plasma mass spectrometry (ICP-MS/MS) combined with a single DGA resin column. The chemical behaviors of Np/Pu and Am/Cm on the DGA resin were consistent in 8-10 mol/L HNO3 and 0.005-0.02 mol/L NaNO2 when 242Pu and 243Am were selected as tracers for Np/Pu and Am/Cm yield monitoring. Based on their different reaction rates with O2, 237Np, 239,240,241Pu, 241Am, and 244Cm in the same solution were simultaneously measured by ICP-MS/MS in the same run. The elimination efficiency of 238U+ tailing (7.43 × 10-9), 238U1H16O2+/238U16O2+ (8.11 × 10-8) and cross contamination of 241Pu and 241Am (<1%) were achieved using 10.0 mL/min He-0.3 mL/min O2 even if the eluate was directly measured without any evaporation. The detection limits of transuranium nuclides were at the femtogram level, demonstrating the feasibility of ICP-MS/MS for simultaneous transuranic radionuclides urinalysis. The developed method was validated by analyzing the spiked urine samples.

Single Slope Single Ramp Column ADC with Digital CDS for CMOS Image Sensors

Single Slope Single Ramp Column ADC with Digital CDS for CMOS Image Sensors

Damage and energy absorption behavior of CFRP/aluminum hybrid open-section thin-walled columns subjected to quasi-static loading

In this study, the damage and energy absorption behaviors of carbon fiber reinforced plastic (CFRP)/aluminum (Al) hybrid open-section thin-walled columns were explored. The results showed that hybrid columns exhibited tremendous merits for the energy absorption. The CFRP-Al columns could absorb 61.21 % and 44.11 % more energy than the corresponding single CFRP columns and the single Al columns with the same thickness, respectively. Furthermore, the specific energy absorption (SEA) of CFRP-Al columns was 16.29 % and 85.06 % higher than that of the corresponding single CFRP columns and the single Al columns. However, different configurations of hybrid columns underwent various damage and failure processes. In addition to Al-CFRP columns, the open-section thin-walled structure exhibited a positive hybrid interaction effect, which dissipated 45.6 % of the total energy absorption. The Al inner layer absorbed a large amount of energy by inducing the CFRP to undergo large deformation and progressive damage, thus, the CFRP-Al columns and CFRP-Al-CFRP columns dissipated more energy and had a higher energy absorption efficiency. Concurrently, the energy absorption behavior of the hybrid column could be changed by different proportions of 45° layers. The present research on damage and energy absorption mechanisms provides some guidance for the lightweight and crashworthiness design of hybrid open-section thin-walled columns.

Repeated Aqueous Film-Forming Foams Applications: Impacts on Polyfluoroalkyl Substances Retention in Saturated Soil.

Historical practices at firefighter-training areas involved repeated aqueous film-forming foams (AFFFs) applications, resulting in source zones characterized by high concentrations of perfluoroalkyl and polyfluoroalkyl substances (PFAS). Repeated applications of AFFF composed of 14 anionic and 23 zwitterionic perfluoroalkyl substances (PFAS) were conducted on a single one-dimensional saturated soil column to quantify PFAS retention. An electrofluorination-based (3M) Milspec AFFF, which was above the mixture's critical micelle concentration (CMC), was at application strength (3%, v/v). Retention and retardation of PFAS mass increased with each successive AFFF addition, although the PFAS concentration profiles for subsequent applications differed from the initial. Greater degree of mass retention and retardation correlated with longer PFAS carbon-fluorine chain length and charged-headgroup type and as a function of AFFF application number. Anionic PFAS were increasingly retained with each subsequent AFFF application, while zwitterionic PFAS exhibited an alternating pattern of sorption and desorption. Surfactant-surfactant adsorption and competition during repeat AFFF applications that are at concentrations above the CMC resulted in adsorbed PFAS from the first application, changing the nature of the soil surface with preferential sorption of anionic PFAS and release of zwitterionic PFAS due to competitive elution. Applying a polyparameter quantitative structure-property relationship developed to describe sorption of AFFF-derived PFAS to uncontaminated, saturated soil was attempted for our experimental conditions. The model had been derived for data where AFFF is below the apparent CMC and our experimental conditions that included the presence of mixed micelles (aggregates consisting of different kinds of surfactants that exhibit characteristics properties different from micelles composed of a single surfactant) resulted in overall PFAS mass retained by an average of 27.3% ± 2.7% (standard error) above the predicted values. The correlation was significantly improved by adding a "micelle parameter" to account for cases where the applied AFFF was above the apparent CMC. Our results highlight the importance of interactions between the AFFF components that can only be investigated by employing complex PFAS mixtures at concentrations present in actual AFFF at application strength, which are above their apparent CMC. In firefighter-training areas (AFFF source zones), competitive desorption of PFAS may result in downgradient PFAS retention when desorbed PFAS become resorbed to uncontaminated soil.

Comprehensive Single-Platform Lipidomic/Metabolomic Analysis Using Supercritical Fluid Chromatography-Mass Spectrometry.

Supercritical fluid chromatography (SFC) is a rapidly expanding technique in the analysis of nonpolar to moderately polar substances and, more recently, also in the analysis of compounds with higher polarity. Herein, we demonstrate a proof of concept for the application of a commercial SFC instrument with electrospray ionization-mass spectrometry (MS) detection as a platform for the comprehensive analysis of metabolites with the full range of polarities, from nonpolar lipids up to highly polar metabolites. The developed single-platform SFC-MS lipidomic/metabolomic method is based on two consecutive injections of lipid and polar metabolite extracts from biphase methyl tert-butyl ether extraction using a diol column and two different gradient programs of methanol-water-ammonium formate modifier. Detailed development of the method focused mainly on the pressure limits of the system, the long-term repeatability of results, and the chromatographic performance, including optimization of the flow rate program, modifier composition and gradient, and injection solvent selection. The developed method enabled fast and comprehensive analysis of lipids and polar metabolites from plasma within a 24 min cycle with two injections using a simple analytical platform based on a single instrument, column, and mobile phase. Finally, the results from SFC-MS analysis of polar metabolites were compared with widely established liquid chromatography MS analysis in metabolomics. The comparison showed different separation selectivity of metabolites using both methods and overall lower sensitivity of the SFC-MS due to the higher flow rate and worse chromatographic performance.

Direct Shear Tests on Soft Clay Reinforced with Single Ordinary Granular Column: Discrete Element-Finite Difference Method

Direct Shear Tests on Soft Clay Reinforced with Single Ordinary Granular Column: Discrete Element-Finite Difference Method

Research on the technology of gob-side entry retaining by pouring support beside the roadway in “three soft” coal seam: A case study

This paper's goal is to investigate if a gob-side entry retention technique combined with a surrounding rock support system is feasible in three soft coal seams. Field engineering confirmed the results of numerical simulation tests and similar simulation tests, which were conducted in accordance with the actual geological conditions of Zhaojiazhai Mine. The following conclusions are reached after studying the technology and process parameter of the gob-side entry retaining in three soft coal seams in conjunction with theoretical calculations: the coal seam of Zhaojiazhai Coal Mine's 12 209 working face is a part of the soft coal seam, and its loose circle is approximately 1.8 m. The expansion roadway size is 3.5 m, and the potential loose circle range is 1.32 m, according to the same model and numerical simulation test. The support scheme after the expansion of the road working face is determined to be the “anchor rod + anchor cable + hydraulic lifting shed” support method. Furthermore, this article suggests a building method for the reinforcement and enlargement of gob-side entry retaining in three-soft thick coal seam by theoretical analysis and numerical simulation. Roadway shotcrete, advance grouting, building of a large deformation anchor cable and continuous resistance, single column lifting shed, hydraulic lifting shed, and roadway enlargement in advance are all steps in the procedure. Furthermore, an analysis is conducted on the deformation features of the surrounding rock in gob-side entry retention. The study highlights the significance of actively supporting the surrounding rock, fortifying the roof support, guaranteeing the stiffness compatibility between the shoulder filling body and the surrounding rock on the roof, boosting the wall's strength and stability, and enhancing the roadway's stability.

Physical Modeling of a Soft Soil Improved with Stone Columns

Abstract This paper reports on an experimental study conducted on a soft cohesive soil improved with stone columns. The load–deformation behavior of the soil specimen has been captured by varying the number of columns, foundation shape, and stones’ particle gradation. A uniform soil bed has been prepared under controlled density and moisture conditions inside a cubic steel tank and stone columns have been installed. Keeping the vertical compressive load, length, and diameter of stone columns constant, five different stone sizes of uniform gradations having average particle sizes ranging between 1.5 and 11 mm have been examined. Load tests are performed using square- and circular-shaped footing plates, and the physical models have been subsequently simulated using the finite element method. The analysis of results revealed that the foundation shape has insignificant effect on current small-scale model testing, but it may be significant at magnified scale. With the increase in particle size, a better reduction in settlement could be achieved, thus implying better improvement. Observed results indicated settlement of a single column is governed by both punching and bulging of column, whereas in a group, the major settlement takes place because of punching into the soil. Nevertheless, this study provides verified and comprehensive frameworks for both physical and numerical model testing of different soils improved with stone columns that would directly benefit practitioners to optimize a site-specific soil improvement program involving the use of stone columns.

Image Object and Scene Recognition Based on Improved Convolutional Neural Network

In recent years, due to the continuous optimization of network structure and the emergence of large-scale data, Convolutional neural network has made breakthroughs in a series of applications of computer vision. Based on this, the Convolutional neural network is improved and optimized. The improved convolutional neural network is introduced into image Object detection and scene recognition, and image object detection is carried out by combining sliding window fusion and Convolutional neural network. The image scene recognition model is constructed by using potential object area recognition and Convolutional neural network Transfer learning. Using different data sets to verify the algorithm, the research results show that in Group1 and Group2, the error rate of the multi column convolutional neural network fused by sliding window is reduced by about 25% compared with the single column convolutional neural network. As the group with the smallest decrease in error rate, Group3 also achieved a 9% decrease in error rate. The fitness rate of object detection algorithm is gradually stable after 7 runs, reaching about 9.8%, and its operation effect is obviously better than other algorithms. The multi column convolutional neural network fused by sliding window is more adaptive to the training data set, and gets better recognition effect in the algorithm operation. However, the image scene recognition model based on potential object area recognition algorithm and Convolutional neural network has good convergence. The average recognition time for image scenes is 1.5356s. The recognition speed is fast and stable, which can effectively solve the problem of multi-scale image scene recognition.

Study The Behavior of Square Reinforced Concrete Columns Strengthened with CFRP

There is a scarcity of research regarding the efficacy of Fiber Reinforced Polymer (FRP) confinement on low strength materials. This paper presents the findings of an experimental study that focuses on the behavior of concentrically loaded short concrete columns with low concrete strength, which are confined using CFRP wraps. A set of nine (9) square concrete columns of short length were subjected to testing. The experimental setup consisted of a single unconfined column, referred to as the control column, and eight additional columns that were confined utilizing externally bonded CFRP wraps. These confining schemes were determined based on the findings of a previous study conducted by the authors, which focused on short square columns with smaller cross sections. The study focused on examining the impact of various confinement schemes on the load carrying capacities of columns through the application of concentric uniaxial compression. The implementation of various confinement schemes led to an increase in the load carrying capacities of confined concrete columns, demonstrating the effectiveness of externally bonded CFRP wraps in enhancing the performance of short rectangular concentrically loaded concrete columns. The primary objective of this investigation is to examine the behavior of concrete columns with reinforcement that have been reinforced using carbon fiber composites. The findings demonstrated the efficacy of carbon fibers in the restoration of impaired columns, as evidenced by a notable enhancement in the load-bearing capacity of the columns, ranging from 35% to 90%.

Collapse-resistant mechanisms induced by various perimeter column damage scenarios in RC flat plate structures

This paper aims at assessing the impact of removing various numbers of perimeter columns on the integrity of RC flat plate substructures. Compared to interior columns, those at the corner and edge positions on the building perimeter are more likely to damage by accidental and abnormal loads, which in turn may cause progressive collapse of the entire structure. Given the uncertainty of the loading events, the likelihood of multiple perimeter columns being simultaneously destroyed is also high. However, existing research has primarily focused on the single column removal cases. To address this research limitation, a 3D high-fidelity finite element model of a flat plate substructure with a missing corner column (named Model C) was developed. The accuracy of the model was validated based on our previous experiment of the same substructure. The validated model was subsequently modified to cover two and multiple perimeter column removal scenarios. The two new models are namely, Model C_1e in which a corner column and an adjacent edge column were damaged and Model C_2e, with a corner column and two adjacent edge columns being removed. The simulation results reveal that flexural action was the primary load resisting mechanism in all three models. The load carrying capacity with three column removals was reduced by 75.6% compared to the removal of a single corner column. Full-depth cracks were found at the cut-off position of the top slab rebars for the columns adjacent to the removed one(s). In Model C and Model C_2e, cracks exhibited a diagonal axisymmetric failure pattern of the corner of the slab, with equal load transfer to two adjacent columns. In contrast, Model C_1e exhibited a diagonal non-axisymmetric failure pattern of the slab corner, with more loads being transferred to the adjacent column along the shorter bay. The energy-based dynamic response analyses were also performed to obtain the Dynamic Increase Factor (DIF) for the substructure models under different column removal scenarios. Based on the nonlinear regression analysis, the DIFs for the substructures with one and two column removals were found to be ranged between 1.22 and 1.51, and 1.28–1.68, respectively.

Bracing requirements and design for a single column considering semi-rigid connections and initial curvature

The use of bracing has been widely recognized as an effective technique to increase the strength of columns by reducing their effective length. This paper presents an assessment of the bracing requirements for a semi-rigidly connected column, which is laterally braced at the mid-height. By utilizing the proposed half-length column model, the effects of column stiffness and initial curvature on the brace strength and stiffness requirements are investigated. The results indicate that the column’s initial curvature coefficient increases with a higher applied load, but decreases with increased column end connection stiffness. The required brace stiffness requirement stipulated in AISC 360-22 should be increased when considering the effect of column’s initial curvature. Following the design concepts of AISC 360-22 and CSA S16-19, equations for assessing the bracing requirements for a semi-rigidly connected column are proposed. These equations are validated against the finite element analysis results, demonstrating their ability to accurately assess the bracing requirements for semi-rigidly connected columns. These equations can be adopted in current engineering practice.

Imaging Classification by Column and Type of the Generalized Distal Radius Die-punch Fractures

Background:: Generalized distal radius die-punch fractures (GDP) can involve three columns. However, there is no three-column classification for GDP. The aim of this study was to introduce a three-column classification for GDP, and to investigate the application effect of the classification. Methods:: 613 patients with GDP accrued from January 2013 to December 2021 were classified by column and fracture type based on imaging findings. First, the GDP was categorized into single-, double-, or three-column fractures. Second, the intermediate column fractures were divided into volar, dorsal, split, collapse, or mixed types; the radial column fractures were divided into metaphyseal, articular or mixed types; and the ulnar column fractures were divided into apical or basal types of the styloid process. The intra- and inter-observer consistency between the two assessors was analyzed with kappa statistics. 227 patients with less fracture displacement were treated conservatively, whereas 386 patients with increased fracture displacement were treated surgically, and the selection of surgical approaches and fracture reduction-fixation methods was guided by the classification. The differences in incidence, gender, age, treatment methods, and functional recovery were compared among the three categories, and the characteristics of different types were observed. The wrist joint function was evaluated according to the Sarmiento-modified Gartland- Werley method. Results:: The intra- and the inter-observer kappa coefficients were obtained as ≥ 0.810. There were significant differences in the incidence (12.7%, 68.5%, and 18.8%, respectively) and age (39.8, 46.6, and 47.1 years, respectively) for single-, double- and three-column fractures (P&lt;0.05). However, there was no significant difference in terms of gender among the three (P&gt;0.05). The mixed, collapse, split, dorsal, and volar types accounted for 28.7%, 27.7%, 21.9%, 15.5%, and 4.5%, respectively. The metaphyseal, articular surface, and mixed types accounted for 35.9%, 33.1%, and 31.0%, respectively. The apical and base types were 81.7% and 18.3%, respectively. Among the cases of volar and dorsal types, the missed diagnosis rate of X-ray alone was 28.5%, but it was confirmed by subsequent CT. In the last follow-up of 12.2 months (range, 6 to 24 months), the excellent plus good rate was 82.7% and 82.6%, respectively, in total and among patients undergoing surgical treatment; the excellent plus good rate of the single column fractures was 96.2%, significantly higher than that of double- and three-column fractures (80.0% and 83.5%, respectively) (P&lt;0.05). All patients with fair or poor wrist function had collapse or mixed-type intermediate column fractures. Conclusion:: Different categories/types of GDP have significant differences in terms of incidence, age, efficacy, and prognosis. Imaging classification by column and type of GDP better reflects the features and injury mechanism of the fractures with good consistency. Therefore, it has important reference value for the surgical modality and prognosis evaluation.

Spacing effects on blast loading characteristics of two tandem square columns under planar shock waves

To effectively evaluate the structural responses, it is crucial to possess relevant knowledge regarding the blast load on the structure. The loading characteristics of two columns differ from those of a single column due to complex flow-field interference. The spacing effect on blast loading characteristics of two tandem rigid square columns is examined numerically and experimentally for different center spacing between the columns (L = 1.2B–12B, where B represents the side length of a column). This includes studying drag characteristics, distribution patterns of reflection overpressure and impulse, and understanding the physical mechanism under blast load. The experimental tests use a shock tube device powered by high-pressure gas, while numerical simulations solve Euler equations using computational fluid dynamics techniques. Our findings reveal that spacing cases can be categorized into four situations: small and medium spacings (L/B = 1.2–2 and 2.5–4), where the upstream column exhibits a significant shielding effect on the downstream column, resulting in the reduced drag coefficient for the downstream column; large spacings (L/B = 5–7), where drag coefficient improves but remains lower than that of a single column; superlarge spacings with negligible shielding effect (L/B = 8–12), leading to similar blast loading for the downstream column compared to a single column.