Microbial Fuel Cell (MFCs) are devices which produces electricity by anaerobic fermentation from organic/inorganic matter, its simple structure and the usage of biodegradable substances as a fuel makes it a potential technology for electricity generation. However, its low performance and high cost of components are obstructing the commercialization of MFC. While designing MFC reactor electrodes combination and substrate play a significant role in the performance. To improve MFC efficiency, tremendous research has been carried out to explore cost effective electrode material. Usually factors like price, reusability, neutrality and increment in output power are considered for material selection. This paper discusses about the various carbonous electrode combination to study about the optimum performance of soil based MFCs, on paddy field. The V-I characteristics is obtained for various MFCs setup to identify the suitable electrode for the chosen substrate. This comparative study is made with the single chamber MFC without proton exchange membrane which is compensated with the usage of terracotta pot. The entire study is carried out with the experimental setup to detect the impact of electrode in power generation for a given substrate.