BackgroundTo treat anterior cruciate ligament (ACL) injuries, double-bundle ACL reconstruction has been proposed as a more anatomical approach relative to single-bundle reconstruction. However, controversy remains over which technique is superior in addressing knee instability, particularly rotational laxity. We hypothesize that double-bundle reconstruction better restores rotational knee laxity, while both methods are similar in restoring anterior knee laxity, to intact knee levels. MethodsA controlled laboratory study. Eight cadaveric knees were tested accordingly: (1) static anterior laxity testing under 150 N-anterior tibial loading at 20°, 60° and 90° knee flexion using a material testing machine, followed by (2) dynamic simulated pivot-shift with knee-specific loading involving iliotibial band forces, valgus and internal rotation torques, while the knee was brought from extension to 90° flexion on a 6°-of-freedom custom-designed rig. Tibiofemoral kinematics were recorded using an electromagnetic tracking system for the ACL-intact, ACL-deficient, single-bundle and double-bundle ACL-reconstructed knee conditions. FindingsDouble-bundle reconstruction reduced internal rotation under pivot-shift to levels not significantly different from ACL-intact conditions (P > .173), unlike single-bundle that remained significantly higher at 10–40° flexion (P < .05). For anterior laxity, there was no significant difference between double-bundle, single-bundle, and ACL-intact conditions under static testing (P > .175) or pivot-shift (P = .219). The maximum extent of knee envelope laxity was significantly reduced for double-bundle relative to single-bundle, particularly for the rotatory component (P = .012). InterpretationDouble-bundle was biomechanically superior to single-bundle in addressing envelope of rotation, while both techniques restored anterior knee laxity to ACL-intact levels.