Single Anchor Research Articles

An experimental and numerical comparative study on the uplift capacity of single granular pile anchor and rough pile in sand

ABSTRACT Granular pile anchor (GPA) is a relatively new foundation technique that is found to be effective in resisting uplift forces in expansive soils. GPA can be utilized as an economical substitute to other methods to resist the uplift loads in sandy soils. In this study, the performance of GPA and rough piles installed in the sand bed under uplift was examined using laboratory-scale model tests and numerical modelling under field-scale conditions. The laboratory-scale model tests were performed on GPA and rough pile installed in loose, medium dense, and dense sand beds by varying the embedment ratio. A numerical study was also done to check the findings from the experimental study and GPA suitability under field-scale conditions. The laboratory-scale and numerical study revealed that GPA performs better than bored pile under uplift force and can be used as an effective ground anchoring alternative.

Smart antenna with circular polarization for high accuracy indoor localization system

This article presents a new switched beam antenna (SBA) for indoor localization applications. The SBA consists of seven circularly polarized (CP) element antennas using a novel feeding network. The attractive characteristics of the proposed CP antenna are high gain, wide bandwidth (BW) with the BW of 36.2% at the reflection coefficient less than −10 dB (4.6–6.7 GHz), the 3 dB axial ratio (AR) BW of 22.4% (5.4–6.7 GHz), wide 3 dB AR beamwidth as about 60 degrees, and maximum gain of 9 dBi within the total bandwidth. Experimental results show good agreement with simulated ones in terms of radiation pattern, AR, and reflection coefficient. We proposed a low-cost and simple RF-based indoor localization system based on a single anchor node equipped with this SBA. The system is demonstrated through experimental validations in a 6 m × 6.5 m × 3.5 m office room with a mean localization error of 0.72 m.

Do Career Demands and Career Choices Always Coincide? A Matching Perspective Based on Career Anchors and Job Characteristics

Career choice is an important behavior for people wanting to develop their social life and is a key link to doing so. The matching of career choice with an individual’s real work demands will have a significant impact on the development of individuals, organizations, and society. However, at this stage, there are few studies on this matching situation. From the perspective of the matching of career anchors and job characteristics, this study explored the distribution and different characteristics of employees’ career demands and their career choices through a survey of 407 employees, and further discussed the matching status of these. The results of the study are as follows: (1) Individual career demands (career anchors) presented three attributes: single, multiple, and unclear. Among the single career anchor types, life anchors had the largest proportion, while among multiple career anchor individuals, individuals with both challenge anchors and service anchors accounted for the majority. (2) Individual career demands (career anchors) were significantly different across most demographic variables and organizational/work variables. (3) Deviations between employees’ career demands and their career choices seemed to be a common phenomenon, with the highest degree of fit (62.79%) with the entrepreneurial creativity anchor and the lowest degree of fit (21.28%) with the lifestyle anchor. In addition, in an analysis of three job fit characteristics, the entrepreneurial creativity anchor had a significant preference for managerial characteristics, whereas the challenge anchor had a significant preference for technological characteristics. The degrees of fit of the other anchors were characterized by the frequency of ‘right suboptimal fit’ being larger than that of ‘left suboptimal fit’. In other words, a specific career anchor had a significant preference for job characteristics matched by the right career anchor, with the midpoint of the career anchor octagon model defining the angle of observation. This study provides a reference for human resource management departments and for employees’ recognition and planning of career anchors.

Uplift resistance capacity of anchor piles used in marine aquaculture

Anchor piles are widely used in marine aquaculture, and the safety is largely determined by the uplift resistance capacity,especially in harsh ocean environments. However, there are few practical guides to the design and installation of the anchor piles for mooring the body of marine aquaculture equipment. Laboratory experiments were conducted to investigate the effect of the initial tension angle, pile diameter, embedded depth, and pile configuration on the uplift resistance capacity of anchor piles under oblique loads. CCD camera and load cell were utilized to measure the corresponding displacement and load, respectively. The results show that increasing the initial tension angle of circular and square single piles can significantly improve the uplift resistance capacity. The failure load of the square single pile was slightly higher than that of the circular single pile. Increasing the pile diameter can effectively improve the failure load and delay the development speed of the pile top displacement. Increasing the embedded depth can effectively improve the failure load and increase the lateral displacement of the pile top. The uplift resistance capacity of the dual anchor piles was better than that of the single anchor piles. The layout configuration has little effect on the failure load, but has a large effect on the displacement development.

Harmonizing the Mooring System Reliability of Multiline Anchor Wind Farms

Abstract One of many barriers to the deployment of floating offshore wind turbines is the high cost of vessel time needed for soil investigations and anchor installation. A multiline anchor system is proposed in which multiple floating offshore wind turbines (FOWTs) are connected to a single caisson. The connection of multiple FOWTs to a single anchor introduces interconnectedness throughout the wind farm. Previous work by the authors has shown that this interconnectedness reduces the reliability of the FOWT below an acceptable level when exposed to survival loading conditions. To combat the reduction in system reliability an overstrength factor (OSF) is applied to the anchors functioning as an additional safety factor. For a 100 turbine wind farm, single-line system reliabilities can be achieved using the multiline system with an OSF of 1.10, a 10% increase in multiline anchor safety factors for all anchors in a farm.

Evaluating the behavior of expandable multi-plate mechanical anchors in granular soils

Many offshore and onshore platforms are constructed widely, especially in the energy supply sector. So far, various anchors with different shapes have been introduced for these applications to reinforce and stabilize the superstructures and related facilities. This study deals with the novel Expandable Multi-plate Mechanical Anchors (EMPMAs) and investigates their performance using physical modeling tests. Previous studies have focused on the behavior of single anchors, and there is no comprehensive experimental research on the multi-plate anchors, which is the subject of the present study. This research examines uplift capacity regarding the effect of five soil densities for loose, medium, and dense conditions and the vertical distance between two plate anchors from 0.5B to 2B. The obtained results were compared with the single plate anchor with equivalent areas. Based on the results, the highest bearing capacity is related to the EMPMA with an optimum distance of 1B in 80% relative density. In addition, by decreasing the distance between two plates, the relative density becomes a more dominant bearing capacity parameter. Furthermore, the Particle Image Velocimetry (PIV) method results are presented, and a new formula is proposed. Eventually, a comparative study is conducted between the results of PIV and an analytical formula.

Reliable Clinical and Sonographic Outcomes of Subpectoral Biceps Tenodesis Using an All-Suture Anchor Onlay Technique

To evaluate the clinical outcomes and structural integrity of primary subpectoral biceps tenodesis using an all-suture anchor onlay technique for long head of the biceps (LHB) tendon pathology. We conducted a retrospective case series with prospectively collected data of patients who underwent primary, isolated subpectoral biceps tenodesis with a single all-suture anchor onlay fixation between March 2017 and March 2019. Outcomes were recorded at a minimum follow-up of 12 months based on assessments of the American Shoulder and Elbow Surgeons (ASES) score, LHB score, and elbow flexion strength and supination strength measurements. The integrity of the tenodesis construct was evaluated using ultrasound. Thirty-four patients were available for clinical and ultrasound examination at a mean follow-up of 18 ± 5 months. The mean ASES score significantly improved from 51.0 ± 14.2 points preoperatively to 89.8 ± 10.5 points postoperatively (P < .001). The minimal clinically important difference for the ASES score was 8.7 points, which was exceeded by 31 patients (91.2%). The mean postoperative LHB score was 92.2 ± 8.3 points. Regarding subcategories, an average of 47.2 ± 6.3 points was reached for "pain/cramps"; 26.4 ± 6.1 points, "cosmesis"; and 18.6 ± 2.6 points, "elbow flexion strength." Both elbow flexion strength and supination strength were similar compared with the nonoperated side (P= .169 and P= .210, respectively). In 32 patients, ultrasound examination showed an intact tenodesis construct, whereas 2 patients (5.9%) sustained failure of the all-suture anchor fixation requiring revision. Primary subpectoral biceps tenodesis using an all-suture anchor onlay technique for pathology of the LHB tendon provides reliable clinical results and a relatively low failure rate (5.9%). Level IV, case series.

EFFECT OF ANCHORAGE REBARS APPLYING TO MID-STORY PIN COLUMN BASE CONNECTION ON STRESS TRANSFER MECHANISM AND ULTIMATE SHEAR STRENGTH UNDER HORIZONTAL FORCE

Mid-story pin column system has been proposed to realize beam yielding mechanism of a steel moment-resisting frame. In this system, a pin connection system is applied inbetween the upper steel column and the lower RC column extended from the base beam to control moment demands on both ends of the first story column. An exposed-type column base has been adopted as a pin connection connected by a single anchor bolt. Static cyclic horizontal loading tests of a partial frame consisting of the first story column and the second floor beams showed a frame with the proposed column base system exhibited stable hysteresis performance up to a story drift angle of 0.05 rad, remaining column in elastic. In addition, the RC column with the connection of a single anchor bolt exhibited slippage at the connection while this problem was solved when an anchor bolt was stiffened by cross-shaped four shear plates. A cover plate was also welded on the top of the shear plates to cover the top surface of the RC column from bearing pressure of the upper steel column. An anchor bolt with the welded plates are called an anchor bolt set, or ABS, hereinafter. Ensuring a reliable seismic performance of an upper steel frame, damages at the proposed connection have to be minimized. So far, compressive stress transfer mechanism was clarified, and the ultimate compressive strength of the connection was evaluated in the previous study.

A Prospective Observational Study on Short-Term Functional Outcome of Arthroscopic Anterior Cruciate Ligament Repair of Proximal Tears Using Knotless Single Suture Anchor Technique.

To assess the clinical outcomes in patients with anterior cruciate ligament (ACL) proximal tears undergoing arthroscopic primary repair with knotless single suture anchor technique. The first twenty-two consecutive patients with proximal ACL tears (Sherman types I and II and high-grade partial tears) treated with arthroscopic primary repair with single suture anchor technique were evaluated until 6months post-operatively. Patients were evaluated with validated functional outcome measures (IKDC and Lysholm scores) and clinical tests for ACL stability. At 6-month follow-up, 91% of patients (n = 20) achieved excellent outcome measures for IKDC and Lysholm scores and had complete stability of the ACL to clinical testing. Two patients with poor outcomes at six weeks; one with subjective instability and the other underwent revision surgery represented a failure rate of 9%. The median Lysholm score was 96 (IQ range, 96-100) and median IKDC subjective score was 87.40 (IQ range, 78.20-88.50) at 6-month follow-up. The improvement in Lysholm and IKDC scores over a period of six months post-operatively was statistically significant when compared to preoperative scores (p ≪ 0.0001). Maximum improvement in clinical outcomes is achieved in the first 6weeks post-surgery with a slower increase thereafter, a time interval which may be considered as a figurative yield point for future work in this field. Arthroscopic ACL primary repair with knotless single suture anchor technique provides excellent short-term clinical outcomes in a carefully selected subset of patients with proximal ACL tears. More powered and longer duration studies are needed to understand longer term outcomes. Level IV, therapeutic case series.

Transcriptome analysis of sesame-Macrophomina phaseolina interactions revealing the distinct genetic components for early defense responses.

The online version contains supplementary material available at 10.1007/s12298-021-01039-6.

Post-installed anchors in concrete strengthened by post-installed reinforcement under tensile load

This research discusses post-installed anchors strengthened by post-installed reinforcement (PRs) under tensile loading. Both types of adhesive and expansion anchors were tested under tensile loading. The PRs are reinforcing bars that were installed in a vertical and an inclined direction around a single anchor, as different samples separately. In general, the results showed that the PRs in the vertical direction were more effective than those in the inclined direction. It was found that the tensile capacity of the adhesive anchors significantly improved using the PRs. Although, no major enhancement was recorded in the tensile capacity of the expansion anchors. For both types of anchors, the PRs prevented severe concrete breakout failure. Moreover, with the PRs, a higher ductile behavior was observed at both types of anchors. Employing PRs could be a feasible and practical method to strengthen anchorage systems in concrete and prevent severe damages in concrete substrates.

The 360 Double Lasso Loop for Biceps Tenodesis: Tips and Tricks

The management of the intra-articular portion of the long head of the biceps tendon (LHB) is a recurring topic in every discussion about shoulder pain. In massive rotator cuff tears or in tears of the superior third of the subscapularis tendon, our approach is to systematically perform a tenodesis of the LHB. In this Technical Note, we present our arthroscopic technique for LHB tenodesis at the articular margin of the humeral head using a single anchor and a 360 double lasso loop. This technique guaranties a strong and efficient fixation of the biceps tendon and is reproducible when following the steps and tips and tricks outlined herein.

Parametric study on Sheet Pile Walls in Layered Soils

Sheet pile wall is a retaining structure. It is used to retain the deep vertical cuts made in the earth, waterfront structures, cofferdams, and bridge abutments. The geotechnical engineering advantage of sheet pile wall is that it does not consume large base width like a retaining wall. When land acquisition problem arises, sheet pile walls are preferred. Sheet pile walls are subjected to rotational failure and flexural failure in layered soil. The rotational failure can be avoided by providing adequate depth of penetration. The in-front foundation soil generates passive force against the active or driving force from backfill soil. The flexural failure is avoided by providing one or more anchors at a particular location on the sheet pile, which in return reduces the maximum bending moments, the maximum shear force developed on the sheet pile and the depth of penetration. This makes the design of anchored sheet pile walls more effective in layered soil. This paper considers soft clay and loose sand as backfill, and stiff clay and dense sand as foundation soil. The outcomes of this research are cantilever sheet pile requires more depth of penetration to avoid rotational failure. For a height of ditch 6m, single anchor is sufficient but for a height of ditch 10m, double anchor required to reduce the depth of penetration, maximum bending moment, shear force, and horizontal deflection.

A Novel Static Path Planning Method for Mobile Anchor-Assisted Localization in Wireless Sensor Networks

Background: Many applications of Wireless Sensor Networks (WSNs) require awareness of sensor node’s location but not every sensor node can be equipped with a GPS receiver for localization, due to cost and energy constraints especially for large-scale networks. For localization, many algorithms have been proposed to enable a sensor node to be able to determine its location by utilizing a small number of special nodes called anchors that are equipped with GPS receivers. In recent years a promising method that significantly reduces the cost is to replace the set of statically deployed GPS anchors with one mobile anchor node equipped with a GPS unit that moves to cover the entire network. Objectives: This paper proposes a novel static path planning mechanism that enables a single anchor node to follow a predefined static path while periodically broadcasting its current location coordinates to the nearby sensors. This new path type is called SQUARE_SPIRAL and it is specifically designed to reduce the collinearity during localization. Results: Simulation results show that the performance of SQUARE_SPIRAL mechanism is better than other static path planning methods with respect to multiple performance metrics. Conclusion: This work includes an extensive comparative study of the existing static path planning methods then presents a comparison of the proposed mechanism with existing solutions by doing extensive simulations in NS-2.

Outcomes and Material Cost Comparison of Transosseous Versus Suture Anchor Fixation of the Achilles Tendon: A Retrospective Study

Insertional Achilles tendinopathy is a common pathology that may be resistant to conservative treatment requiring surgical intervention. Treatment often involves partial to complete detachment of the Achilles tendon, debridement, and reattachment. Although 50% of the tendon may be safely resected without significantly compromising strength, the addition of a lengthening procedure requires further reinforcement. We conducted a retrospective review of 43 procedures comparing outcomes of 16 single suture anchor procedures with 27 transosseous fixation procedures for reattachment of the Achilles. The suture anchor group utilized one of 2 different industry anchors while the transosseous group utilized sutures ranging in size from 2-0 to #2. Female patients contributed 67% of the procedures with 53% occurring on the left side. For each group the median preoperative pain score (scale 0-10) was 8 and the median of the lowest reported postoperative pain score was 0. The typical time to lowest postoperative pain was 10 weeks for the suture anchor group and 4 weeks for the transosseous group. Tourniquet time averaged 59.3 (12.9) minutes for the suture anchor group and 65.1 (16.4) for the transosseous group. There was a large difference in material costs between the suture anchor and transosseous groups which ranged from $364.51 to $448.51 and $99.80 to $104.00 respectively. With similar results on postoperative pain and adverse event rate to suture anchor fixation and lower material costs than anchor fixation, transosseous fixation remains a viable option for fixating the Achilles tendon to bone in treatment of insertional Achilles tendinopathy.

Load transfer mechanism of a load distributive compression anchor installed in weathered rock layer

The demand for a load distributive compression anchor (LDCA) in construction sites has been increasing, owing to its high bearing capacity and removable steel strands. As an LDCA comprises multiple anchor bodies and unbonded steel strands, the load applied to the strands generates a distributive compressive stress in the grout, preventing high concentration of grout–ground shear stress. Unlike in the conventional anchors, in an LDCA, independent load transfer of each anchor body induces interference effect between adjacent anchor bodies. Therefore, for an efficient design of an LDCA, it is necessary to investigate the load transfer mechanism considering the effect of multiple anchor bodies. In this study, a series of anchor pull-out field tests was conducted for LDCAs consisting of a single anchor body, and double and triple anchor bodies spaced by 1, 2, and 3 m. According to the test results, the LDCA showed a stiffer load–displacement behavior with an increase in the number and spacing of the anchor bodies. The multiple anchor bodies of the LDCA generated an overlapping of the grout axial load and induced its rapid dissipation, increasing the grout–ground shear stress. This interference effect was more clearly observed with a decrease in the anchor body spacing.

SUBSTITUTION OF ANCHOR CURRENCY: CHALLENGES FOR TRADE BETWEEN IRAN AND ITS MAJOR TRADING PARTNERS

The aim of the paper is to find out whether euro is a convenient substitution for U.S. dollar as an anchor currency for Iranian rial and whether this replacement would affect Iran’s international trade positively. We explore these effects via Optimum Currency Area (OCA) theories using generalized least square from 2000 to 2018. Based on OCA index, euro would be a good substitution for U.S. dollar as an anchor for Iranian rial. In addition, gravity model and Generalized Method of Moments estimation confirm that substitution of U.S. dollar by euro would improve bilateral trade between Iran and its major trade partners especially the European Economic and Monetary Union (EMU). Furthermore, we confirm that a basket containing main currencies (euro, U.S. dollar, yuan, Russian rubble) would be more efficient than a single currency anchor however euro should be prominent in the basket. Such a change of anchor could positively contribute to reduction of transaction costs, diversification of external risk, rise of mutual trade exchanges between Iran and the EMU or the EU and consequent economic growth of trade partners. The paper contributes to the existing literature by comprehensive methodological approach how to identify an appropriate anchor currency.

Do Techniques for Hill-Sachs Remplissage Matter in Terms of Functional and Radiological Outcomes?

Background:Different techniques are used for the remplissage procedure, including the double-pulley and mattress suture techniques. Both techniques have shown good results; however, it is unclear if one technique is superior.Hypothesis:The remplissage procedure using the double-pulley technique with 2 anchors would have superior functional and radiological outcomes compared with the mattress suture technique with a single anchor.Study Design:Cohort study; Level of evidence, 3.Methods:This study included patients with anterior shoulder instability who were treated using arthroscopic Bankart repair combined with remplissage between 2012 and 2017. A structured questionnaire was used to gather information on the following metrics: Instability Severity Index Score, hyperlaxity, Sugaya index, presence of a Hill-Sachs defect, number of dislocations before surgery, sports participation, radiological measurement of the Hill-Sachs lesion, postoperative range of motion in both shoulders, Rowe score, Walch-Duplay score, American Shoulder and Elbow Surgeons score, and Filling Index Score of Remplissage grade according to magnetic resonance imaging scans at the last follow-up.Results:There were 41 patients included with a mean age of 30 ± 7 years who underwent the Hill-Sachs remplissage procedure using the double-pulley technique with 2 anchors (n = 21; group DA) or the mattress suture technique with a single anchor (n = 20; group SA). At the final follow-up, there were no significant differences between the groups regarding the Instability Severity Index Score (P = .134), the Sugaya index (P = .538), sports participation (P = .41), the radiological measurement of the Hill-Sachs lesion (P = .803), or the Rowe score (P = .182). However, there were significant differences between the groups in the Walch-Duplay score (P = .012), American Shoulder and Elbow Surgeons score (P = .005), and Filling Index Score of Remplissage grade (P = .015), favoring group DA, as well as differences in external rotation in a neutral position (external rotation loss: 9° ± 3° [group SA] vs 12° ± 3° [group DA]; P = .003) and at 90° of abduction (external rotation loss: 8° ± 3° [group SA] vs 11° ± 3° [group DA]; P = .006), favoring group SA.Conclusion:In the remplissage procedure, the double-pulley technique provided better filling of the lesion and improvement in functional scores, but external rotation was limited compared with the mattress suture technique.

Short-Term Clinical and Return-to-Work Outcomes After Arthroscopic Suprapectoral Onlay Biceps Tenodesis With a Single Suture Anchor

PurposeTo describe short-term outcomes of arthroscopic suprapectoral onlay biceps tenodesis using a single all-suture anchor with respect to validated outcome measures, return to work, objective strength and motion data, and biceps-specific testing.MethodsThis study describes a consecutive series of patients undergoing arthroscopic suprapectoral onlay biceps tenodesis performed by a single surgeon from January to December 2017. Patients were evaluated preoperatively and postoperatively with the American Shoulder and Elbow Surgeons questionnaire, visual analog scale, Single Assessment Numeric Evaluation, Veterans RAND 12-Item Health Survey, and 12-Item Short Form survey, and return-to-work survey. Postoperative strength, range of motion, and biceps-specific testing was also performed.ResultsThis study included 50 patients (26 men and 24 women), with an average age (± standard deviation) of 50.1 ± 10.9 years and average final follow-up of 21.3 ± 8.5 months. Among employed patients, 32 (71.1%) returned to work at an average of 4.6 ± 2.3 months. Light-duty workers returned to work at a significantly greater rate (85.7% vs 33.3%, P = .016) and in less time (2.6 ± 2.0 months vs 6.8 ± 4.2 months) than heavy-duty workers. No differences were found between operative and nonoperative sides in the biceps apex distance (P = .636) or range of motion in elbow flexion and extension (P > .9 for both), supination (P = .192), or pronation (P = .343) postoperatively. Strength in elbow flexion (P = .002), as well as shoulder forward elevation (P < .001) and external rotation (P < .001), increased postoperatively. Significant patient-reported improvements were noted in the American Shoulder and Elbow Surgeons score, visual analog scale pain score, Single Assessment Numeric Evaluation score, Constant-Murley score, and Veterans RAND 12-Item Health Survey and 12-Item Short Form physical component scores (P ≤ .001 for all). A postoperative Popeye deformity developed in 5 patients (10%).ConclusionsArthroscopic suprapectoral onlay biceps tenodesis with a single all-suture anchor can provide overall excellent clinical outcomes regarding strength, motion, and validated patient-reported outcome questionnaires. Return to occupational activities may be less predictable and more prolonged for heavy laborers. A small number of patients may experience cosmetic deformity postoperatively.Level of EvidenceLevel IV, therapeutic case series.

Characterization of Membrane Topology and Retention Signal of Pestiviral Glycoprotein E1.

ABSTRACTPestiviruses are members of the family Flaviviridae, a group of enveloped viruses that bud at intracellular membranes. Pestivirus particles contain three glycosylated envelope proteins, Erns, E1, and E2. Among them, E1 is the least characterized concerning both biochemical features and function. E1 from bovine viral diarrhea virus (BVDV) strain CP7 was analyzed with regard to its intracellular localization and membrane topology. Here, it is shown that even in the absence of other viral proteins, E1 is not secreted or expressed at the cell surface but localizes predominantly in the endoplasmic reticulum (ER). Using engineered chimeric transmembrane domains with sequences from E1 and vesicular stomatitis virus G protein, the E1 ER-retention signal could be narrowed down to six fully conserved polar residues in the middle part of the transmembrane domain of E1. Retention was observed even when several of these polar residues were exchanged for alanine. Mutations with a strong impact on E1 retention prevented recovery of infectious viruses when tested in the viral context. Analysis of the membrane topology of E1 before and after the signal peptide cleavage via a selective permeabilization and an in vivo labeling approach revealed that mature E1 is a typical type I transmembrane protein with a single span transmembrane anchor at its C terminus, whereas it adopts a hairpin-like structure with the C terminus located in the ER lumen when the precleavage situation is mimicked by blocking the cleavage site between E1 and E2.IMPORTANCE The shortage of specific antibodies against E1, making detection and further analysis of E1 difficult, resulted in a lack of knowledge on E1 compared to Erns and E2 with regard to biosynthesis, structure, and function. It is known that pestiviruses bud intracellularly. Here, we show that E1 contains its own ER retention signal: six fully conserved polar residues in the middle part of the transmembrane domain are shown to be the determinants for ER retention of E1. Moreover, those six polar residues could serve as a functional group that intensely affect the generation of infectious viral particles. In addition, the membrane topology of E1 has been determined. In this context, we also identified dynamic changes in membrane topology of E1 with the carboxy terminus located on the luminal side of the ER in the precleavage state and relocation of this sequence upon signal peptidase cleavage. Our work provides the first systematic analysis of the pestiviral E1 protein with regard to its biochemical and functional characteristics.