Simultaneous Infusion Of Glucose Research Articles

Beziehungen zwischen glukose- und ketonkörperstoffwechsel bei eviscerierten, nephrektomierten ratten und der effekt von oxalacetat, citrat und pyruvat auf die periphere ketonkörperaufnahme

The effect of acetoacetate on the peripheral uptake of glucose was explored in eviscerated, nephrectomized male Sprague-Dawley rats. When 550 mg glucose per kg per h were infused intravenously over a period of 4h, 88% of the infused glucose were taken up by the peripheral tissues. In experiments with simultaneous infusion of 550 mg glucose per kg per h and 384 mg sodium acetoacetate per kg per h the peripheral uptake of glucose was with 93% rather higher than lower compared with the experiments without acetoacetate. The glycogen content of the diaphragm was 0.070 ± 0.022 % wet weight in the experiments without acetoacetate and 0.059 ± 0.017 % wet weight in the experiments with simultaneous acetoacetate infusion. In the experiments with simultaneous infusion of glucose and acetoacetate the peripheral uptake of total ketone bodies could be significantly enhanced by a single intravenous injection of sodium citrate (50 mg/kg) or sodium oxaloacetate (50 mg/kg). The effect of citrate was significantly stronger than the effect of oxaloacetate. The intravenous injection of 100 mg sodium pyruvate per kg had no effect on the peripheral uptake of ketone bodies, while the single injection of 100 mg glucose per kg short acting but distinctly enhanced the peripheral uptake of ketone bodies.