Simultaneous Extraction Research Articles

Microalgal synthesis of the biodiesel employing simultaneous extraction and esterification via heterogeneous catalyst

Within the realm of biorefinery, the third generation of biodiesel production relies on extracting oil from Algae to create renewable fuels. Algae cultivation can occur under precise laboratory conditions, making them a sustainable substitute for Fatty Acid Methyl Ester or biodiesel production. Through analysis of the simultaneous extraction and (trans)esterification process of Algae Chlorella Minutissima utilizing sulfated carbonaceous catalysts, scientists have discovered an efficient technique for producing biodiesel. Various parameters, such as catalyst loading, oil-to-methanol ratio, reaction temperature, and reaction time for FFA reduction and FAME yields, were studied and optimized. Results indicate that utilizing a 10 wt% catalyst loading and a 70:30 volumetric oil to methanol ratio can achieve 67.59 % FFA reduction and a biodiesel yield of 44 mL. This method of producing biodiesel can occur in a single-unit operation, leading to higher yields of renewable fuel at a reduced cost and energy consumption. Finally, the quality of the biodiesel produced met ASTM D6751 standards, confirming its suitability for use.

Single inductor multi-PZTs SECE and electromagnetic voltage multiplier rectifier hybrid interface circuit

This paper proposes a single inductor multi-piezoelectric transducers (PZTs) SECE and electromagnetic voltage multiplier rectifier hybrid interface circuit (MP-SECE-VMR), which harvest energy from multi-input PZTs and electromagnetic generator (EMG). The MP-SECE-VMR adopts a voltage multiplier rectifier circuit, utilizing the diode-connected MOSFET configuration which used as diodes to effectively reduce voltage losses during rectification. Experimental tests confirm that this circuit efficiently extracts energy from PZTs despite differences in their phase relationships. The low input voltage from the EMG is effectively elevated through the voltage multiplier rectifier, enabling simultaneous extraction with the PZTs. The proposed MP-SECE-VMR circuit extracts 1.2 mW of power from two PZTs with open-circuit amplitudes of 9 V and 11 V respectively, as well as from an EMG input with an open-circuit amplitude of 3 V and a frequency of 5 Hz. Furthermore, the proposed circuit effectively enhances output power while expanding the working load range compared to single-source energy extraction.

Simultaneous extraction of Saponin and Polysaccharide from Acanthopanax senticosus fruits with three-component Deep eutectic solvent and the extraction mechanism analysis

As the deep eutectic solvent extraction has the advantages of excellent dissolving capability, high extraction efficiency and selectivity, a three-component deep eutectic solvent (3c-DES) was prepared with betaine, triethanolamine and MgCl2·6H2O (betaine: triethanolamine: MgCl2·6H2O = 1: 4: 0.08, molar ratio), and was used for simultaneous extraction of Acanthopanax polysaccharide and saponin from Acanthopanax senticosus fruits.Response surface methodology (RSM) was employed to optimize the saponin extraction factors. Under the optimum condition that material-liquid (AS powder to 3c-DES) mass ratio was 1:25 g/g, water content was 29 %, extraction temperature was 49 °C, extraction time was 3.5 h, the yield of saponin and polysaccharide were 1.04 and 3.40 wt%, respectively. The effect order on saponin is temperature > time > material-liquid ratio > water content. A quadratic polynomial parameter mathematical model was established to predict the experimental results. Molecular dynamics combined with density functional theory was used to analysis the mechanism of polysaccharide and saponin extraction that the main driving force is strong hydrogen bonding. The hydrogen bonds among deep eutectic solvent and polysaccharides and saponins is between 3.101 Å and 1.817 Å. The deep eutectic solvent could effectively simultaneously extract the saponins and polysaccharides of Acanthopanax senticosus fruits.

CTCNet: A CNN Transformer capsule network for sleep stage classification

In this paper, we propose a novel neural network architecture called CTCNet. First, we adopt a multi-scale convolutional neural network (MSCNN) to extract low and high-frequency features, adaptive channel feature recalibration (ACFR) to enhance the model’s sensitivity to important channel features in the feature maps and reduce dependence on irrelevant or redundant features, a multi-scale dilated convolutional block (MSDCB) to capture characteristics of different types among feature channels. Second, we use Transformer to extract global temporal context features. Third, we employ capsule network to capture spatial location relationships among EEG features and refine these features. Besides, the capsule network module is used as our model’s classifier to classify the final results. It is worth noting that our model better solves the problem that previous researches failed to take into account the simultaneous extraction of local features and global temporal context characteristics of EEG signals, and ignored the spatial location relationships between these features. Eventually, we assess our model on three datasets and it achieves better or comparable performance than most state-of-the-art methods.

Efficient and recycled magnetic self-stirring hollow fiber-solid/liquid phase system: An extraction and removal technique for personal care pollutants

The removal of personal care products (PCPs) in aqueous environments is a huge challenge. Traditional powder and magnetic materials are not conducive to complete solid–liquid separation, which hinders their practical application. In this study, the convenient recyclable magnetic self-stirring system combining with n-octanol hollow fiber-liquid phase micro-extraction (HF-LPME), wormhole-like mesoporous carbon (WMC) solid phase and magnetic stirring was constructed to improve the extraction and removal efficiency of the trace PCPs (triclosan, triclocarban, 2, 2′-Dihydroxy-4-methoxybenzophenone, and 2, 4-dihydroxydiphenylketone) in wastewater. The adsorption process of triclosan and 2,2′-dihydroxy-4-methoxybenzophenone on the WMC surface followed the pseudo-first-order kinetics as well as Freundlich isotherm model. Because WMC had oxygen-containing groups and the multiple mesoporous distribution forming the n-octanol microextraction unit and the shearing strength generated by the stir bar in the water sample accelerated the mass transfer efficiency of PCPs, this system had the advantage of simultaneous removal and extraction of four PCPs from wastewater samples. The extraction mechanism involved the hydrophobic force and size effect between the WMC and PCPs. In addition, the factors affecting extraction efficiencies (EEs) were discussed, such as the concentrate of WMC (1 mg/L), rotation rate (400 rpm), extraction time (15 min), and salt concentration (30%). The results indicated that the method possessed a wide linear range (0.05–200 μg L-1), and lower limits of detection (LODs) (S/N = 3, 0.005–0.05 μg L−1), at the optimal conditions. This method also showed excellent reusability and stability, with higher recovery (88–98%) and fewer relative standard deviations (2.4–6.3%). This work not only minimized secondary pollution from powder adsorbents, but provided an economic and green magnetic self-stirring system and absorbent for the extraction, removal, and determination of typical PCPs pollutants from actual wastewater samples.

Simultaneous extraction of oil, protein and polysaccharide from residual seed cake of Camellia oleifera Abel. using three phase partitioning

The seed cake of Camellia oleifera Abel. (SCOC), a big residue produced after Camellia oil extraction, enriches with many bioactive components. However, it is often discarded directly due to the lack of cost-effective extraction. Herein, a three-phase partitioning (TPP) system is first time tried for the simultaneous extraction of three major ingredients i.e. residual oil, protein, and polysaccharide from SCOC. Results from the extraction yields show that the established method is more efficient or at least comparable to conventional techniques. The developed method takes only 1.5 h and can be carried out at mild temperature of 40 °C, making it a cost-effective method. The successful scaling up of the extraction system at a 30 L extraction tank confirms its scalability. Furthermore, compared with conventional extraction methods, the TPP extracts shows better product quality. These facts, along with simultaneous extraction features of the method, allow us to use TPP as a promising extraction technology of SCOC biomolecules.

Synergistic application of alum sludge and sequential extraction for phosphorus recovery from sewage sludge char

Phosphorus (P) recovery from sewage sludge (SS) (or its ash/char) is challenging due to the presence of heavy metals (HMs) in SS. This study proposed the integration of SS, alum sludge (AS), sequential wet extraction, and pyrolysis for P recovery without simultaneous HM extraction. SS + AS mixture (P:Al molar ratio = 3:1) was first acid pretreated to obtain amended SS, then pyrolyzed, and finally, P was extracted from the char using an alkali. Pyrolysis was conducted at low (400 °C), medium (600 °C), and high (900 °C) temperatures. The highest alkaline P recovery efficiency was 88 % from the amended SS char, while 32 % from the unamended SS char (both prepared at 400 °C). Overall, the highest P recovery in the amended process was 48 % higher than the unamended process despite 11 % P loss during acidic pretreatment. Standards measurements and testing (SMT) protocol and solid-state NMR (31P and 27Al) analyses revealed that acidic pretreatment of SS with AS induced apatite phosphate (AP) to non-apatite inorganic phosphate (NAIP) conversion in SS. As NAIP is more soluble in alkali than AP and HMs, higher alkaline P recovery was achieved for amended SS char than unamended SS char, without HM contamination. However, higher pyrolytic temperatures impaired P recovery due to the immobilization of P in the char carbon matrix and the formation of recalcitrant P phases. These findings were corroborated by complementary characterization techniques such as XPS, XRD, and FTIR. Some major HMs in SS and AS were concentrated in char, except Zn, which partially volatilized above 600 °C. This study demonstrated the applicability of AS for extracting P from SS, offering a potential pathway for the synergistic management of sludge waste streams.

Laparoscopic cholecystectomy with non-X-ray endoscopic retrograde cholangiopancreatography for simultaneous common bile duct stone extraction.

Laparoscopic cholecystectomy with non-X-ray endoscopic retrograde cholangiopancreatography for simultaneous common bile duct stone extraction.

In situ Transesterification of Crambe Seeds with Dimethyl Carbonate Using an Enzymatic Catalyst

In situ transesterification of crambe seeds oil was investigated to produce fatty acid methyl esters using dimethyl carbonate as acyl acceptor and lipase Novozyme 435® as the catalyst. First, the effects of solvent-to-seeds ratio, seed pretreatment, and extraction time were evaluated on the seed oil removal, comparing the results with conventional Soxhlet extraction. Then, the effects of enzyme loading (5 to 30 wt.% based on the oil mass) and reaction time (60 to 540 min) were evaluated on the simultaneous extraction and reaction. The highest oil extraction yield (ca. 26 wt.%) was achieved at the solvent-to-seeds ratio of 6 mL g-1 and 360 min. The oil obtained from seeds that received thermal pretreatment showed a higher concentration of minor compounds. The highest ester content (76.71 wt.%) was obtained with an enzyme loading of 20 wt.%, solvent-to-seeds ratio of 6 mL g-1, for 480 min. The reaction samples showed a predominance of erucate and oleate esters, with identification of the co-products formation (glycerol carbonate and glycerol dicarbonate), and ca. 5 wt.% of acylglycerols.

OPTIMIZING CROP MANAGEMENT AND PRODUCTION WITH ARTIFICIAL INTELLIGENCE DATA MINING USING 3D CONVOLUTIONAL NEURAL NETWORK FOR PRECISION AGRICULTURE

In precision agriculture, optimizing crop management is essential for sustainable and efficient food production. This research leverages artificial intelligence (AI) data mining techniques, specifically employing a 3D CNN, to enhance precision in wheat crop production. The background underscores the need for advanced technologies in agriculture to address the challenges of increasing global demand and environmental sustainability. The method involves the utilization of 3D CNN for simultaneous feature extraction and prediction, providing a holistic approach to crop monitoring. The contribution of this research lies in the integration of AI-driven data mining to streamline crop management processes, resulting in improved resource utilization and increased yield. The application of 3D CNN demonstrated superior performance in accurately predicting wheat crop production. The model effectively extracted intricate spatial and temporal features, contributing to enhanced decision-making capabilities for farmers. The findings highlight the potential of AI-driven precision agriculture in revolutionizing crop management, offering a scalable solution for sustainable food production.

A Randomised Split-Mouth Clinical Trial to Assess the Efficacy of Oroquiver: An Affordable Vibrotactile Device for Dental Injections

Introduction: Injection pain is a common concern among patients, including those receiving dental injections. Local Anaesthesia (LA) is a crucial procedure in dentistry to ensure painless treatment. Vibration stimuli have shown promise in raising the pain threshold, managing orofacial pain, musculoskeletal pain, and relieving dental pain. However, many vibrotactile devices available in the market are not affordable for common dentists. Aim: To compare pain perception in patients undergoing bilateral extractions using OroQuiver-assisted LA administration versus conventional LA administration. Materials and Methods: A randomised split-mouth clinical trial was conducted with 17 patients undergoing simultaneous bilateral extractions. LA was administered with and without OroQuiver on the right and left-sides, respectively. Pain assessment was performed using Wong Baker’s Facial Rating Scale and a questionnaire sheet comparing patient comfort following LA administration on both sides. Results: There was a significant difference in the Visual Analog Scale (VAS) score for pain experienced during LA injections using the vibrotactile device (4.29±1.1) compared to the conventional method (7.2±1.5). None of the patients reported discomfort with the device. The device reduced anxiety towards dental injections in 14 patients, and 16 patients indicated they would recommend the device to others. Conclusion: OroQuiver effectively reduced injection-related pain regardless of the type of nerve block administered. It is a costeffective, easily maneuverable, feasible, sterilizable, and reusable device that effectively reduces LA injection-related pain.

Synthesis of a functionalized and photodegradable fluorene-based polymer for aqueous SWNT dispersion

Due to their tunability, conjugated polymers have received significant interest in the dispersion of single-walled carbon nanotubes (SWNTs). Indeed, interactions with conjugated polymers enable simultaneous solubility, extraction, and purification of...

Upgrading AquaSolv Omni (AqSO) biorefinery: access to highly ethoxylated lignins in high yields through reactive extraction (REx)

Ethanol-mediated reactive extraction is a green and simple process to simultaneously extract and functionalize lignin from wood. Ethoxy groups were incorporated in up to 40.8/100 Ar and the lignins were found suitable for adhesive formulations.

Asymmetric Evaporation for Efficient and Simultaneous Extraction of Freshwater, Salt, and Electrical Energy from Seawater

The simultaneous extraction of freshwater, salt, and energy from seawater using solar interfacial evaporation methods still faces significant challenges. Here, a novel asymmetric evaporation model is proposed. This asymmetric evaporation...

Preparation of a new composite based on multilayer fullerene with mesoporous carbon nitride and its application in the extraction of tacrolimus and everolimus from plasma prior to LC-MS/MS analysis.

The development of new and efficient adsorbents for dispersive solid-phase extraction method, particularly prior to chromatography analysis, is increasing. In particular, this method is recommended for use before biological sample analysis. In this work, a new composite was prepared from mesoporous carbon nitrides and carbon nano-onions and was utilized for the extraction of tacrolimus and everolimus from plasma samples prior to high-performance liquid chromatography-tandem mass spectrometry analysis. To achieve this aim, first, mesoporous carbon nitrides and carbon nano-onions were synthesized separately and mixed at optimized proportions. Subsequently, a suitable amount of the prepared composite (5 mg) was added to 2 mL of sample solution containing the analytes under vortexing. Next, the extracted analytes were eluted using acetonitrile. The approach was linear within the ranges of 1.0-500 and 0.51-500 ng mL-1 for tacrolimus and everolimus, respectively. Sensitive limits of detection (0.31 and 0.15 ng mL-1 for tacrolimus and everolimus, respectively), acceptable relative standard deviations (intra- and inter-day precisions of ≤5.6% and high extraction recoveries of 71.0% and 83.0% for tacrolimus and everolimus, respectively) were obtained. The results showed that the method can be successfully applied in the simultaneous extraction of the studied analytes from plasma.

A novel extraction method for three ochratoxins in human urine based on polystyrene/polyethersulfone electrospun nanofibers coated with copper nanoparticles.

In this study, four types of nanofibers were prepared via electrospinning and characterized through SEM, TEM, EDS, FTIR, TG, XPS and water contact angle analyses, and the novel polystyrene/polyethersulfone nanofibers coated with copper nanoparticles (PS/PES-CuNPs nanofibers) were developed as an ideal adsorbent for the extraction of three ochratoxins from human urine. The solid-phase extractant of sample pretreatment displayed preferable sensitivity and an extraction effect, and the analytical method based on the novel packed-fiber solid-phase extraction strategy followed by high performance liquid chromatography-fluorescence detection (PFSPE-HPLC-FLD) achieved an exceedingly low limit of detection (LOD) and limit of quantification (LOQ) of 0.108-0.162 μg L-1 and 0.658-0.701 μg L-1, respectively; a high spiked recovery of 71.3-92.0% and a lower adsorption time of 7 min, thus demonstrating excellent results compared with other reported adsorbents for ochratoxins from various samples. With the application of this method for the detection of ochratoxins in human urine samples, six in thirty samples were tested positive. This study confirmed that the PS/PES-CuNP nanofibers and PFSPE showed promising potential as a sensitive method for simultaneous extraction and detection of ochratoxins in complex samples.

Integrated cellular 4D-TIMS lipidomics and transcriptomics for characterization of anti-inflammatory and anti-atherosclerotic phenotype of MyD88-KO macrophages.

Introduction: Recent progress in cell isolation technologies and high-end omic technologies has allowed investigation of single cell sets across multiple omic domains and a thorough exploration of cellular function and various functional stages. While most multi-omic studies focused on dual RNA and protein analysis of single cell population, it is crucial to include lipid and metabolite profiling to comprehensively elucidate molecular mechanisms and pathways governing cell function, as well as phenotype at different functional stages. Methods: To address this gap, a cellular lipidomics and transcriptomics phenotyping approach employing simultaneous extraction of lipids, metabolites, and RNA from single cell populations combined with untargeted cellular 4 dimensional (4D)-lipidomics profiling along with RNA sequencing was developed to enable comprehensive multi-omic molecular profiling from the lowest possible number of cells. Reference cell models were utilized to determine the minimum number of cells required for this multi-omics analysis. To demonstrate the feasibility of higher resolution cellular multi-omics in early-stage identification of cellular phenotype changes in pathological and physiological conditions we implemented this approach for phenotyping of macrophages in two different activation stages: MyD88-knockout macrophages as a cellular model for atherosclerosis protection, and wild type macrophages. Results and Discussion: This multi-omic study enabled the determination of the lipid content remodeling in macrophages with anti-inflammatory and atherosclerotic protective function acquired by MyD88-KO, hence expedites the understanding of the molecular mechanisms behind immune cells effector functionality and of possible molecular targets for therapeutic intervention. An enriched functional role of phosphatidylcholine and plasmenyl/plasmalogens was shown here to accompany genetic changes underlying macrophages acquisition of anti-inflammatory function, finding that can serve as reference for macrophages reprogramming studies and for general immune and inflammation response to diseases.

A novel magnetite C18/paracetamol/alginate adsorbent bead for simultaneous extraction of synthetic antioxidants and bisphenol A in water samples

A novel magnetite C18/paracetamol/alginate adsorbent bead for simultaneous extraction of synthetic antioxidants and bisphenol A in water samples

Extraction and separation of anthocyanins from Kushui rose by ethanol-(NH4)2SO4 aqueous two-phase system.

Simultaneous extraction of anthocyanins and removal of sugars from Kushui rose was performed using an ethanol-ammonium sulphate aqueous two-phase system (ATPS). The effects of different parameters, such as type of salt, concentrations of salt and ethanol, temperature and pH on the partition coefficient and recovery of anthocyanins in the top system and sugars in the bottom system were studied. Furthermore, an experimental design of a three-level three-factor Box-Behnken design response surface methodology (RSM) was used to obtain optimal extraction conditions. The maximum partition coefficient (5.64) and recovery (78%) of anthocyanins in the top system within the investigated range were obtained at 22% (w/w) concentration of ammonium sulphate, 25% (w/w) concentration of ethanol, pH 5 and 33.5 °C. During the discussion of the main factors, the maximum recovery of sugars reached 70.09%. The HPLC profile of anthocyanins obtained from the ATPS top phase was similar to that of anthocyanins extracted by ethanol, which indicated that the ethanol-ammonium sulphate ATPS was suitable for the extraction of anthocyanins. On the basis of the anthocyanin stability experiment, anthocyanins extracted from Kushui rose should be stored at low pH and temperature.

Simultaneous Bilateral Phacoemulsification and Cataract Extraction - A New Operation Method

Simultaneous Bilateral Phacoemulsification and Cataract Extraction - A New Operation Method