Simultaneous Ablation Research Articles

Bifunctional CuS composite nanofibers via in situ electrospinning for outdoor rapid hemostasis and simultaneous ablating superbug

Outdoor sports or combat missions, such as jungle adventures or counter-piracy operations, often involve bleeding and infected wounds. Due to the poor and limited medical conditions in these outdoor situations, patients prefer an easy way to simultaneously treat blood loss and wound infection by using the same outdoor device and the same material. In this study, a portable electrospinning device (150 g in weight) for outdoor use was developed, and CuS composite nanofibers could be green-synthesized by this portable electrospinning device. These CuS composite nanofibers can be deposited in situ onto the wound to simultaneously achieve rapid hemostasis outdoors and ablate superbacteria without requiring the use of other materials or devices. These in situ electrospun nanofibers possess better compactness on the rough wound surface than conventional nanofiber mats pressed onto the wound site. Thus, the nanofiber not only accelerates the hemostasis (<6 s) but also shortens the healing time of the wound with superbacterial infection (18 days). These bifunctional nanofibers combined with a portable electrospinning device for outdoor rapid hemostasis and simultaneous superbug ablation could notably benefit the outdoor first aid.

Persistent Atrial Fibrillation Ablation Using Circular Irrigated Ablation Catheter

Introduction: Persistent atrial fibrillation (PsAF) ablation remains time consuming, even when guided by non-invasive mapping. We investigated the role of a multielectrode irrigated circular radiofrequency (RF) catheter for PsAF ablation. Methods: A circular catheter (nMARQ®, Biosense Webster, Inc) with 10 irrigated simultaneous mapping and ablation electrodes was used in 50 patients with PsAF (age 60 ± 11 years, left atrial (LA) size 21.8 ± 9.7 cm2 and atrial fibrillation (AF) maximum duration 10.6 ± 9.3 months). Ablation was guided by non-invasive mapping (ECUVETM, Cardioinsight Inc.) in 32 (64%) patients. Pulmonary vein isolation (PVI) was systematically performed. Results: After targeting additional non-PV regions including 3 ± 2 indicated by ECVUETM (1-6), AF terminated in 37 (74%) patients, into atrial tachycardia (AT) in 22 and directly into sinus rhythm (SR) in 15 patients. Thirteen patients were in SR during the procedure. PVI required 8.01 ± 5.27 minutes of RF. Eleven patients required direct current (DC) shock to terminate AF. Linear lesions were performed for AT: 14 LA roof lines, 13 mitral isthmus lines and 27 cavo tricuspid isthmus (CTI). Using total of 20.24 ± 17.25 minutes of RF. Interestingly, 5/8 roof line block, 4/5 mitral isthmus line block and 19/24 CTI line block, were blocked with circular catheter only with 5.08 ± 4.58, 3.30 ± 2.56 and 3.26 ± 2.12 minutes of RF duration, respectively. A single tip conventional ablation catheter was required to complete linear lesions and obtain the block in 3 roof lines, 1 mitral isthmus line and 5 CTI. Mean procedure duration was 3.18 ± 1.03 hours. Complications included 1 pericardia effusion managed conservatively and 1 transient ischemic attack (TIA) resolved without any neurological sequelae. One year follow up data was available in 27 (54%) patients, 18 (67%) patients were in SR and 9 (33%) patients had AF recurrence in whom 1 (3%) patient had AT recurrence. Conclusion: Circular radiofrequency ablation catheter demonstrated encouraging results for rapid, safe and effective PsAF ablation when guided by non-invasive phase mapping. The catheter can be used for linear ablations and CTI in addition to PVI, thus obviating the need for an additional ablation catheter in the majority of patients.

Simultaneous Stereotactic Radiofrequency Ablation of Multiple (≥ 4) Liver Tumors: Feasibility, Safety, and Efficacy

PurposeTo assess feasibility, safety, and clinical outcome of simultaneous stereotactic radiofrequency (RF) ablation of multiple (≥ 4) primary and metastatic liver tumors. Materials and MethodsThis retrospective observational study included 92 patients (29 women, 62 men), 35 with ≥ 4 hepatocellular carcinomas (HCCs) and 57 with ≥ 4 metastatic liver tumors at initial stereotactic RF ablation between 2005 and 2018. The median size of 178 HCCs and 371 metastases was 2.2 cm (range, 1.0–8.5 cm) and 3.0 cm (range, 0.5–13 cm), respectively. At initial stereotactic RF ablation, 17 (48.6%) patients with HCC and 19 (33.3%) with metastases had 4 liver tumors, 11 (31.4%) and 19 (33.3%) had 5 tumors, and 7 (20%) and 19 (33.3%) had ≥ 6 tumors. ResultsMajor complications occurred in 2 of 35 ablations (5.4%) in patients with HCCs and in 7 of 63 (10%) with metastases. The primary technical efficacy rate (ie, successful initial ablation) was 100% (178/178) in HCCs and 98.8% (363/371) in metastases. Local recurrence was observed in 4 of 178 (2.2%) HCCs and in 17 of 371 (4.6%) metastases. Overall survival (OS) rates at 1, 3, and 5 years from the date of the first stereotactic RF ablation were 88.0%, 54.0%, and 30.4% for patients with HCCs with a median OS of 38.2 months and 86.1%, 53.1%, and 37.3% for patients with metastases with a median OS of 37.4 months. ConclusionsStereotactic RF ablation is a feasible, safe, and efficacious option in simultaneous management of multiple primary and metastatic liver tumors.

Preparation of Quasi-One-Dimensional Metal Heterostructures by Simultaneous Ablation of Two Targets over Superfluid Helium Surface

The technique of simultaneous coagulation of two different materials in superfluid helium has been implemented, which allows one to create different ultrathin (d < 8 nm) heterostructures. A simultaneous ablation of two metal targets (indium and platinum) immersed in the volume of superfluid helium and targets carried out above the surface of the liquid has been performed. During the ablation of targets in the volume of helium, nanowires from individual metals are obtained. During the ablation of targets located above the surface, in addition to nanowires of individual metals, “combined” nanowires are formed when the wire from indium coaxially overlap the platinum wire.

Intracellular cathepsin C levels determine sensitivity of cells to leucyl-leucine methyl ester-triggered apoptosis.

L-leucyl-leucine methyl ester (LLOMe) is a lysosomotropic detergent, which was evaluated in clinical trials in graft-vs-host disease because it very efficiently killed monocytic cell lines. It was also shown to efficiently trigger apoptosis in cancer cells, suggesting that the drug might have potential in anticancer therapy. Using U-937 and THP-1 promonocytes as models for monocytic cells, U-87-MG and HeLa cells as models for cancer cells, and noncancerous HEK293 cells, we show that the drug triggers rapid cathepsin C-dependent lysosomal membrane permeabilization, followed by the release of other cysteine cathepsins into the cytosol and subsequent apoptosis. However, monocytes were found to be far more sensitive to the drug than the cancer and noncancer cells, which is most likely a consequence of the much higher intracellular levels of cathepsin C-the most upstream molecule in the pathway-in monocytic cell lines as compared to cancer cells. Overexpression of cathepsin C in HEK293 cells substantially enhances their sensitivity to the drug, consistent with the crucial role of cathepsin C. Major involvement of cysteine cathepsins B, S, and L in the downstream signaling pathway to mitochondrial cell death was confirmed in two gene ablation models, including the ablation of the major cytosolic inhibitor of cysteine cathepsins, stefin B, in primary mouse cancer cells, and simultaneous ablation of two major cathepsins, B and L, in mouse embryonic fibroblasts (MEFs). Deletion of stefin B resulted in sensitizing primary murine breast cancer cells to cell death without affecting the release of cathepsins, whereas simultaneous ablation of cathepsins B and L largely protected MEFs against cell death. However, due to the extreme sensitivity of monocytes to LLOMe, it appears that the drug may not be suitable for anticancer therapy due to risk of systemic toxicity.

Microstructural characterization of nanocomposites produced by laser ablation in a magnetic field

We present a microstructural study of nanocomposite samples based on iron oxide and a noble or a transition metal. The samples were produced by laser ablation in air at atmospheric pressure in the presence of a magnetic field. This technology allows production of oriented nanowires composed by nanoparticles. We applied a simultaneous laser ablation of two targets in an external magnetic field. The targets used for the ablation were a magnetic material (iron) and a noble or a transition metal (silver, gold or cobalt). The presence of a magnetic field during the deposition results in arranging the nanoparticles formed in the plasma plume into micrometric nanowires on the substrate. The structural analyses confirms that the samples are composed by iron oxide and a noble or a transition metal.

The roles of polymerases ν and θ in replicative bypass of O6- and N2-alkyl-2′-deoxyguanosine lesions in human cells

Exogenous and endogenous chemicals can react with DNA to produce DNA lesions that may block DNA replication. Not much is known about the roles of polymerase (Pol) ν and Pol θ in translesion synthesis (TLS) in cells. Here we examined the functions of these two polymerases in bypassing major-groove O6-alkyl-2'-deoxyguanosine (O6-alkyl-dG) and minor-groove N2-alkyl-dG lesions in human cells, where the alkyl groups are ethyl, n-butyl (nBu), and, for O6-alkyl-dG, pyridyloxobutyl. We found that Pol ν and Pol θ promote TLS across major-groove O6-alkyl-dG lesions. O6-alkyl-dG lesions mainly induced G→A mutations that were modulated by the two TLS polymerases and the structures of the alkyl groups. Simultaneous ablation of Pol ν and Pol θ resulted in diminished mutation frequencies for all three O6-alkyl-dG lesions. Depletion of Pol ν alone reduced mutations only for O6-nBu-dG, and sole loss of Pol θ attenuated the mutation rates for O6-nBu-dG and O6-pyridyloxobutyl-dG. Replication across the two N2-alkyl-dG lesions was error-free, and Pol ν and Pol θ were dispensable for their replicative bypass. Together, our results provide critical knowledge about the involvement of Pol ν and Pol θ in bypassing alkylated guanine lesions in human cells.

Genetic and pharmacologic modulation of cementogenesis via pyrophosphate regulators.

Pyrophosphate (PPi) serves as a potent and physiologically important regulator of mineralization, with systemic and local concentrations determined by several key regulators, including: tissue-nonspecific alkaline phosphatase (ALPL gene; TNAP protein), the progressive ankylosis protein (ANKH; ANK), and ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1; ENPP1). Results to date have indicated important roles for PPi in cementum formation, and we addressed several gaps in knowledge by employing genetically edited mouse models where PPi metabolism was disrupted and pharmacologically modulating PPi in a PPi-deficient mouse model. We demonstrate that acellular cementum growth is inversely proportional to PPi levels, with reduced cementum in Alpl KO (increased PPi levels) mice and excess cementum in Ank KO mice (decreased PPi levels). Moreover, simultaneous ablation of Alpl and Ank results in reestablishment of functional cementum in dKO mice. Additional reduction of PPi by dual deletion of Ank and Enpp1 does not further increase cementogenesis, and PDL space is maintained in part through bone modeling/remodeling by osteoclasts. Our results provide insights into cementum formation and expand our knowledge of how PPi regulates cementum. We also demonstrate for the first time that pharmacologic manipulation of PPi through an ENPP1-Fc fusion protein can regulate cementum growth, supporting therapeutic interventions targeting PPi metabolism.

Risk of Atrial Fibrillation after Ablation of Cavotricuspid Isthmus-Dependent Atrial Flutter: Is Combined Ablation of Atrial Fibrillation Worthwhile?

ResumoFundamento A ablação da fibrilação atrial (FA) e do flutter atrial dependente de istmo cavo-tricuspídeo (FLA-ICT) pode ser realizada simultaneamente quando as duas arritmias tenham sido registradas antes do procedimento. Entretanto, a melhor abordagem não é clara quando pacientes com FLA-ICT são encaminhados para ablação sem o registro prévio de FA.Objetivos Avaliar a prevalência e identificar os preditores de ocorrência do primeiro episódio de FA após ablação de FLA-ICT em pacientes sem o registro prévio de FA.Métodos Coorte retrospectiva de pacientes submetidos exclusivamente a ablação por cateter para FLA-ICT, sem registro prévio de FA. As características clínicas foram comparadas entre os grupos em que houve ocorrência de FA pós-ablação de FLA-ICT vs. sem ocorrência de FA. O nível de significância estatística adotado foi de 5%. Na análise de preditores, o desfecho primário avaliado foi ocorrência de FA após ablação de FLA-ICT.Resultados De um total de 227 pacientes submetidos a ablação de FLA-ICT (110 com registro de FA e 33 sem seguimento adequado), 84 pacientes foram incluídos, dos quais 45 (53,6%) apresentaram FA pós-ablação. Não houve variáveis preditoras de ocorrência de FA. Os escores HATCH e CHA2DS2-VASC foram semelhantes nos dois grupos. As taxas de recorrência de FLA-ICT e complicações após a ablação foram de 11,5% e 1,2%, respectivamente.Conclusões A ablação de FLA-ICT é eficaz e segura, mas 50% dos pacientes desenvolvem FA após ablação. Entretanto, ainda é incerto o papel da ablação combinada (FLA-ICT e FA) visando prevenção da ocorrência de FA. (Arq Bras Cardiol. 2020; [online].ahead print, PP.0-0)

Hyperactivated PTP1B phosphatase in parvalbumin neurons alters anterior cingulate inhibitory circuits and induces autism-like behaviors

Individuals with autism spectrum disorder (ASD) have social interaction deficits and difficulty filtering information. Inhibitory interneurons filter information at pyramidal neurons of the anterior cingulate cortex (ACC), an integration hub for higher-order thalamic inputs important for social interaction. Humans with deletions including LMO4, an endogenous inhibitor of PTP1B, display intellectual disabilities and occasionally autism. PV-Lmo4KO mice ablate Lmo4 in PV interneurons and display ASD-like repetitive behaviors and social interaction deficits. Surprisingly, increased PV neuron-mediated peri-somatic feedforward inhibition to the pyramidal neurons causes a compensatory reduction in (somatostatin neuron-mediated) dendritic inhibition. These homeostatic changes increase filtering of mediodorsal-thalamocortical inputs but reduce filtering of cortico-cortical inputs and narrow the range of stimuli ACC pyramidal neurons can distinguish. Simultaneous ablation of PTP1B in PV-Lmo4KO neurons prevents these deficits, indicating that PTP1B activation in PV interneurons contributes to ASD-like characteristics and homeostatic maladaptation of inhibitory circuits may contribute to deficient information filtering in ASD.

Ablation strategies for intramural ventricular arrhythmias

Intramural origin of ventricular arrhythmias is one of the reasons for failure of catheter ablation, especially in nonischemic substrates. Conventional unipolar ablation has limited efficacy for the creation of deep transmural lesions in the ventricular myocardium, and alternative ablation strategies have been developed to overcome this problem. These novel approaches include simultaneous unipolar ablation, bipolar ablation, use of low-ionic irrigant solution, needle ablation, and ethanol ablation. This review provides an overview of each one of these techniques, including their main advantages and limitations.

NRF2 negatively regulates primary ciliogenesis and hedgehog signaling.

Primary cilia are lost during cancer development, but the mechanism regulating cilia degeneration is not determined. While transcription factor nuclear factor-erythroid 2-like 2 (NRF2) protects cells from oxidative, proteotoxic, and metabolic stress in normal cells, hyperactivation of NRF2 is oncogenic, although the detailed molecular mechanisms by which uncontrolled NRF2 activation promotes cancer progression remain unclear. Here, we report that NRF2 suppresses hedgehog (Hh) signaling through Patched 1 (PTCH1) and primary ciliogenesis via p62/sequestosome 1 (SQSTM1). PTCH1, a negative regulator of Hh signaling, is an NRF2 target gene, and as such, hyperactivation of NRF2 impairs Hh signaling. NRF2 also suppresses primary cilia formation through p62-dependent inclusion body formation and blockage of Bardet–Biedl syndrome 4 (BBS4) entrance into cilia. Simultaneous ablation of PTCH1 and p62 completely abolishes NRF2-mediated inhibition of both primary ciliogenesis and Hh signaling. Our findings reveal a previously unidentified role of NRF2 in controlling a cellular organelle, the primary cilium, and its associated Hh signaling pathway and also uncover a mechanism by which NRF2 hyperactivation promotes tumor progression via primary cilia degeneration and aberrant Hh signaling. A better understanding of the crosstalk between NRF2 and primary cilia/Hh signaling could not only open new avenues for cancer therapeutic discovery but could also have significant implications regarding pathologies other than cancer, including developmental disorders, in which improper primary ciliogenesis and Hh signaling play a major role.

Transcatheter arterial chemoembolization combined with simultaneous DynaCT-guided microwave ablation in the treatment of small hepatocellular carcinoma

PurposeTo evaluate the method and effectiveness of transcatheter arterial chemoembolization (TACE) combined with simultaneous DynaCT-guided Microwave ablation (MWA) for the treatment of small hepatocellular carcinoma (SHCC).Materials and methodsFrom June 2015 to May 2017, a total of 28 consecutive patients with SHCC received single treatment of TACE and 23 subjects received a combination treatment of TACE with simultaneous DynaCT-guided MWA. Following 1 month of treatment, the tumor response was assessed using the mRECIST criteria and the outcomes were analyzed including intervention-associated complications, changes in liver function, imaging response, and progression-free survival (PFS).ResultsThe technical success rate was 100%. The rates of CR (65%) in the combined TACE and MWA group were higher than those of the TACE group (46%). The rate of common adverse events, such as liver abscess, spontaneous bacterial peritonitis and liver dysfunction, in the combined TACE and MWA group (56%) was comparable to the corresponding rate of the TACE group (P > 0.411). The median and mean PFS of the TACE group were significantly lower than those of the combined TACE and MWA group (19.00 months vs. 29.00 months, 21.076 months vs. 24.693 months, p = 0.019, log-rank test).ConclusionStereotactic DynaCT-guided MWA is a safe and effective method for the treatment of SHCC, which usually provides an effective tumor puncture path, notably for lesions that cannot be detected following TACE. Overall, the data suggested that this treatment method could improve the clinical outcome of patients with SHCC.

DGK α and ζ Activities Control TH1 and TH17 Cell Differentiation.

CD4+ T helper (TH) cells are critical for protective adaptive immunity against pathogens, and they also contribute to the pathogenesis of autoimmune diseases. How TH differentiation is regulated by the TCR's downstream signaling is still poorly understood. We describe here that diacylglycerol kinases (DGKs), which are enzymes that convert diacylglycerol (DAG) to phosphatidic acid, exert differential effects on TH cell differentiation in a DGK dosage-dependent manner. A deficiency of either DGKα or ζ selectively impaired TH1 differentiation without obviously affecting TH2 and TH17 differentiation. However, simultaneous ablation of both DGKα and ζ promoted TH1 and TH17 differentiation in vitro and in vivo, leading to exacerbated airway inflammation. Furthermore, we demonstrate that dysregulation of TH17 differentiation of DGKα and ζ double-deficient CD4+ T cells was, at least in part, caused by increased mTOR complex 1/S6K1 signaling.

CdTe:Sn thin films deposited by the simultaneous laser ablation of CdTe and Sn targets

CdTe:Sn thin films were grown by pulsed laser deposition on glass substrates at room temperature. The study of the changes in the chemical, structural and optical properties of the films as a function of the density of Sn ions calculated from Langmuir planar probe measurments is presented. Four films were grown by the simultaneous ablation of CdTe and Sn targets in vacuum. The Sn ion density was modified by varying the fluence on the Sn target, while the CdTe plasma density was kept constant for all the deposits. One CdTe film was grown as control sample using the same experimental conditions as in the CdTe:Sn films. The chemical composition was analyzed by XPS, where a dependence of the Sn incorporation into the CdTe lattice, on the Sn ion plasma density was demonstrated. The crystalline structure analysis by XRD showed a hexagonal structure for all the films. When the CdTe and Sn plasmas were combined, a preferential orientation in the plane (110) was observed for the resulting films. Furthermore, as Sn plasma density increased, the intensity of the (110) peak increased as well, suggesting that crystalline re-orientation is an effect of Sn incorporated into the CdTe lattice. Vibrational behavior was analyzed by Raman spectroscopy. A vibrational mode appeared at 118 cm−1 and was related to a Sn-Te vibration, suggesting the incorporation of substitutional Sn into the CdTe lattice. The Sn-Te bonding was confirmed by XPS by the appearance of signals centered at 485 and 494 eV, which correspond to Sn2+ in SnTe. Thin films thicknesses values were between 320 and 460 nm increasing with Sn ion density. The band gap calculated using UV–vis spectrophotometry, resulted in values ranging from 1.42 to 1.46 eV. PL measurements showed a slight blue shift of the near-edge emission as Sn plasma density increased.

Laparoscopic colorectal resection combined with simultaneous thermal ablation or surgical resection of liver metastasis: a retrospective comparative study

Objective Colorectal liver metastasis is a critical cause of mortality. However, the safety and long-term prognosis of simultaneous colorectal tumor resection along with hepatic lesion ablation are debated. The current analysis was conducted to further clarify the controversy. Methods In this retrospective study, we collected data of 68 patients who underwent ablation or resection for liver lesions combined with simultaneous laparoscopic primary colorectal tumor resection between September 2011 and October 2016 at the Third Affiliated Hospital of Sun Yat-sen University. Perioperative outcomes and long-term follow-up data were compared between patients in the resection and ablation groups. Results Both groups had similar surgical duration (286.70 ± 78.33 vs. 313.67 ± 80.90 min), conversion rate (2 vs. 0), total expenses (81.51 ± 20.20 vs. 82.21 ± 27.81 kRMB, p = .914) and morbidities (11 vs. 24, p = .667). However, the postoperative hospital stays (12.82 ± 9.25 vs. 8.40 ± 2.38 d) and transfusion rates (56.52% vs. 8.89%) were significantly lower in the ablation group. The long-term overall survival (p = .714), disease-free survival (p = .680) and intra-hepatic recurrent-free survival (p = .496) were comparable between both groups. Conclusion With respect to simultaneous treatment for both primary colorectal cancer and liver metastasis, hepatic lesion ablation was associated with lower blood loss and hospital stay duration than liver resection, without compromising the surgical safety and long-term prognosis.

Simultaneous Surgical Ablation of Atrial Fibrillation with Cardiac Surgery: A Review

Simultaneous Surgical Ablation of Atrial Fibrillation with Cardiac Surgery: A Review

Interaction of Immune Cells and Tumor Cells in Gold Nanorod-Gelatin Composite Porous Scaffolds.

Composite porous scaffolds prepared by immobilization of photothermal nano-agents into porous scaffold have been used for both cancer therapy and tissue regeneration. However, it is not clear how the host immune cells and ablated tumor cells interact and stimulate each other in the composite scaffolds. In this research, a gold nanorod-incorporated gelatin composite scaffold with controlled spherical large pores and well interconnected small pores was fabricated by using ice particulates as a porogen. The composite porous scaffold was used for investigating the interaction between dendritic cells and photothermally ablated breast tumor cells. The composite scaffold demonstrated excellent photothermal property and the temperature change value could be adjusted by irradiation time and laser power density. The composite scaffold showed excellent photothermal ablation ability towards breast tumor cells. The photothermally ablated tumor cells induced activation of dendritic cells when immature dendritic cells were co-cultured in the composite scaffold. Consequently, the gold nanorod–incorporated gelatin composite porous scaffold should provide a useful platform for simultaneous photothermal-immune ablation of breast tumor.

Simultaneous microwave ablation therapy combined with laparoscopic splenectomy plus pericardial devascularization for the treatment of small hepatocellular carcinoma with portal hypertension

本文回顾分析了2013年6月至2017年4月浙江省人民医院(杭州医学院附属人民医院)肝胆胰外科及微创外科小肝癌合并门静脉高压症8例患者资料。患者男性6例，女性2例，年龄(51.6±6.7)岁，均行超声引导微波消融(PMAT)联合腹腔镜脾切除加贲门周围血管离断术(LSPD)治疗。8例患者均顺利完成手术，无中转开腹。手术时间(195.6±62.4)min，其中消融时间(9.6±2.2)min，术中出血量(228.7±101.9)ml，术后住院时间(12.1±4.6) d。无术后严重并发症。肝脏肿瘤消融完全，无消化道再出血。随访2～36个月，1例随访7个月肝癌复发再次消融，随访至今未见复发转移。其余肝癌患者无复发。

B7-H3 as a Novel CAR-T Therapeutic Target for Glioblastoma

Glioblastoma (GBM) remains one of the most malignant primary tumors in adults, with a 5-year survival rate less than 10% because of lacking effective treatment. Here, we aimed to explore whether B7-H3 could serve as a novel therapeutic target for GBM in chimeric antigen receptor (CAR) T cell therapy. In this study, a CAR targeting B7-H3 was constructed and transduced into T cells by lentivirus. Antitumor effects of B7-H3-specific CAR-T cells were assessed with primary and GBM cell lines both in vitro and in vivo. Our results indicated that B7-H3 was positively stained in most of the clinical glioma samples, and its expression levels were correlated to the malignancy grade and poor survival in both low-grade glioma (LGG) and GBM patients. Specific antitumor functions of CAR-T cells were confirmed by cytotoxic and ELISA assay both in primary glioblastoma cells and GBM cell lines. In the orthotropic GBM models, the median survival of the CAR-T-cell-treated group was significantly longer than that of the control group. In conclusion, B7-H3 is frequently overexpressed in GBM patients and may serve as a therapeutic target in CAR-T therapy.