The chiral separation of a pharmaceutical intermediate by a simulated moving bed (SMB) system on a pilot-scale is described. The operating conditions were chosen from results simulated by the software, help, developed by Novasep, based upon data from a single column. The productivity of the SMB system is tested by the separation of an ester of quinoline mevalonic acid at various internal flow-rates. The eluent consumption is also discussed. The step time to switch the ports to enter or withdraw solutions is one of important factors influencing the productivity.