A new Intervertebral Motion Device (IMD) was developed in this study. Depending on its configuration, the IMD was used to measure motion in the sagittal, frontal, and transverse planes. Calibration results showed that the root-mean-square (RMS) error of the IMD was 0.092 mm in axial translation, 0.065 mm in shear translation, and 0.091° in rotation. Using the IMD, nine intact human lumbosacral spine specimens (L3–S1) were tested under a simulated physiological load on an MTS (Model 858 Bionix, MTS System Corporation, Minneapolis, MN). The ranges of motion (ROMs) of intact and instrumented specimens were measured in terms of angular motion (main motion) and coupled translation in the sagittal plane, frontal plane, and angular motion in the transverse plane. The results demonstrated that simulated fusion with CD instrumentation at the level of L4–L5 significantly decreased the ROMs of L4–L5 for all main and coupled motions ( P<0.03). The application of CD rods had less influence on the angular ROM in L/R axial rotation compared to the angular ROMs in Flex/Ext and L/R lateral bending.