Aims: This study aimed to assess the knowledge gain of women farmers participating in a training program on tuber crop cultivation in Thrissur district, Kerala. The training focused on improving their understanding of key agricultural practices related to crops such as cassava, elephant foot yam, coleus, and sweet potatoes. Study Design: Ex post facto design. Place and Duration of Study: The training was organized and conducted by the Center for Gender Studies in Agriculture and Farm Entrepreneurship Development, College of Agriculture, Vellanikkara, Thrissur, Kerala on March 2024. Methodology: A total of 50 women farmers were randomly selected from various panchayats in the Thrissur district to attend the training. A pre- and post-training evaluation was conducted using a structured questionnaire comprising 15 questions, covering topics such as variety selection, soil fertility management, pest and disease control, and post-harvest value addition, and so on. Using grapesAgri1 in R Studio 4.3.1 software, the data were analysed using statistical methods like frequency, percentage, t-test and coefficient of correlation. Results: Pre- and post-training knowledge scores were calculated and analysed using simple percentage formulas to assess knowledge gain. The study revealed a significant improvement in participants' knowledge post-training. The overall average pre-training knowledge score was 9.67 out of 50 (19.34%), while the average post-training score increased to 41.53 out of 50 (83.06%). T-test was also employed to compare the mean of pre-test and post-test evaluation. The percentage of knowledge gain in the pre-training and post-training evaluation were correlated with the independent variables such as age, education, family size, category and information source utilization. The results of correlation revealed that the independent variable such as age (X1), Education (X2), Category (X3) and farm size (X4) are negatively correlated and information source (X5) was positively correlated with the dependent variable. Conclusion: The training program effectively enhanced the knowledge of women farmers in key aspects of tuber crop cultivation, contributing to improved agricultural practices and the potential for increased productivity.