You have accessJournal of UrologyCME1 Apr 2023MP64-02 A PILOT STUDY ON THE DARK MATTER OF CLEAR CELL RENAL CELL CARCINOMA: THE ROLE OF THE URINARY MICROBIOME Andrei D. Cumpanas, Rohit Bhatt, Yi XI Wu, Andrew Brevik, Kalon L. Morgan, Allen Rojhani, ANTONIO R. H. Gorgen, Lilian Xie, Rajiv Karani, Zachary E. Tano, Sohrab N. Ali, Pengbo Jiang, Roshan M. Patel, Jaime Landman, and Ralph V. Clayman Andrei D. CumpanasAndrei D. Cumpanas More articles by this author , Rohit BhattRohit Bhatt More articles by this author , Yi XI WuYi XI Wu More articles by this author , Andrew BrevikAndrew Brevik More articles by this author , Kalon L. MorganKalon L. Morgan More articles by this author , Allen RojhaniAllen Rojhani More articles by this author , ANTONIO R. H. GorgenANTONIO R. H. Gorgen More articles by this author , Lilian XieLilian Xie More articles by this author , Rajiv KaraniRajiv Karani More articles by this author , Zachary E. TanoZachary E. Tano More articles by this author , Sohrab N. AliSohrab N. Ali More articles by this author , Pengbo JiangPengbo Jiang More articles by this author , Roshan M. PatelRoshan M. Patel More articles by this author , Jaime LandmanJaime Landman More articles by this author , and Ralph V. ClaymanRalph V. Clayman More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003322.02AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: With the discovery of the urinary microbiome, the dogma that normal urine is sterile has been reversed. Indeed, we are only now beginning to explore how the urinary microbiome relates to various urologic maladies. Our objective was to compare the urinary microbiome between patients with simple renal cysts (SRC) and patients with a newly diagnosed, clear cell renal cell carcinoma (ccRCC) both before and after removal of the tumor. METHODS: Eighteen ccRCC patients and 17 SRC patients provided an initial urine sample. Nine ccRCC patients provided a postoperative urine sample (median 159 days after surgery) and nine renal cyst patients provided a second urine sample (median 102 days after the first sample). Urine samples underwent 16S rRNA sequencing. Bacterial diversity, defined as the number of observed species (richness), relative abundance (i.e >1% of any of the ten most abundant taxa) and distribution of abundances between groups (evenness), was determined. RESULTS: In the ccRCC group, microbial richness was decreased compared to the SRC group (22.6 vs. 33.5 bacterial species, p<0.01). In the SRC group, there was no difference in richness between the 1st and 2nd urine sample. In the ccRCC group bacterial richness increased post-operatively (Figure 1). In the SRC group, there was increased abundance (i.e. >1%) of Proteus, Streptococcus and Varibaculum while in the ccRCC patient group, there was an increased abundance of Anaerococcus, Corynebacterium, Enterococcus and Gardnerella (Figure 2). In the ccRCC group only, two bacteria had a >2% relative abundance: Gardnerella and Enterococcus. CONCLUSIONS: In the ccRCC group there was a markedly decreased bacterial richness, which increased post-operatively, but failed to equal the bacterial richness noted in the SRC group. Of note, Gardnerella and Enterococcus were particularly notable in the preoperative ccRCC group. Source of Funding: None. © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e880 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Andrei D. Cumpanas More articles by this author Rohit Bhatt More articles by this author Yi XI Wu More articles by this author Andrew Brevik More articles by this author Kalon L. Morgan More articles by this author Allen Rojhani More articles by this author ANTONIO R. H. Gorgen More articles by this author Lilian Xie More articles by this author Rajiv Karani More articles by this author Zachary E. Tano More articles by this author Sohrab N. Ali More articles by this author Pengbo Jiang More articles by this author Roshan M. Patel More articles by this author Jaime Landman More articles by this author Ralph V. Clayman More articles by this author Expand All Advertisement PDF downloadLoading ...