Simple Instructions Research Articles

KM Easy Grammar Training at LKSA Ar Ridho

In learning English, students often find it difficult to learn English grammar. Grammar in English lessons is considered difficult by some of them, making English less popular. Actually, when a teacher can deliver simple grammar instruction, the students will find it much easier to learn and they come to appreciate English. The purpose of this activity is to provide training to caregivers at LKSA Ar Ridho so that foster children there can be helped in understanding Grammar by providing easy Grammar teaching, which is Easy Grammar. The expected output by holding this Easy Grammar training is to increase knowledge about Grammar in English to the caregivers in order that foster children there become happy and enthusiastic in learning English which they initially considered difficult. The community service team along with the foundation's management held a discussion where the results of the discussion agreed that the community service team could organize activities that could help caregivers to be able to teach foster children to learn English easily by studying Easy Grammar. The output target resulting from this community service activity is in the form of knowledge that will be obtained by the caregivers so that they can teach the children in their care.

Abstract P331: Simple Instruction and Provision of Literature on Low Salt Diet Ineffective in Reducing Salt Intake in Patients with Hypertension

Objective: Hypertension remains poorly controlled at the population level. Reduction of salt intake via the DASH diet is effective in reducing blood pressure (BP). We enrolled a cohort of patients with hypertension to assess molecular factors that influence BP control over a follow up period of 6 months. Here we report dietary sodium intake and BP control. Methods: 80 non-diabetic patients aged >40 with hypertension were enrolled in the study. Subjects reccieved brief instruction and a handout on the DASH diet from a study coordinator. Medications were titrated to a BP of <130/80 at enrollment, months 1, 2,3 and 6. BP was measured at each visit using an automated cuff following clinical trial methodology. 24-hour urine sodium excretion was collected at enrollment, months 3 and 6. Descriptive variables were summarized and paired t-tests were used to compare sodium excretion and BP. Results: Cohort characteristics are in Table 1. 73 subjects provided 24-hour urine samples and BP at enrollment and month 3 while 67 had samples at enrollment and month 6. Mean BP at enrollment was 129.9/75.3 (SD 16.6/12.5) which reduced 123/71.8 (SD 12.9/10) at month 3, p <0.001and 125.4/73.4 (SD 15.2/10.5), p=0.02. 24-hour sodium excretion was 152 mmol (SD 73.6) at enrollment. There was no difference in sodium excretion at 3- (154 mmol, SD 86.2, p=0.8) or 6 months (139 mmol, SD 60,, p=0.18). Conclusions: According to 24-hour urine collection, participants consumed roughly 3.5 g of sodium per day. Simple instruction and provision of literature regarding a DASH diet was ineffective in reducing sodium intake. Further study is required to determine a more effective educational delivery method and understand any social factors that may inhibit diet implementation in this urban hypertensive cohort.

Development and validation of the theatre team tool (TTT): A clinical debriefing tool for multidisciplinary theatre teams

Clinical debriefing (CD) after unanticipated clinical events benefits staff, teams, patients and systems. Use of a structured tool may help overcome barriers to implementation, however, there is not presently a validated CD tool for theatre teams. We aimed to design and validate a CD tool for multidisciplinary theatre teams.The initial stages of development involved prototype design using underlying theory and literature, including review of pre-existing CD tools. Content validity evidence was sought from subject matter experts to calculate Item- and Scale-Content Validity Indices (I-CVI; S-CVI) of the tool. Face validity evidence was sought from wider theatre team members to evaluate clarity, relevance, difficulty and sensitivity of the tool. The final stage of development involved integration of these findings to deliver the final version of the CD tool, which was named the “Theatre Team Tool”: “TTT” or “Triple T” as a shorthand.The tool contains simple instructions on how to conduct a CD. A novel design aspect is that it gives options to debrief with either intention to ‘learn’ or ‘manage’ according to the required impact. All sections of the tool achieved an I-CVI greater than the recommended cut-off value. The S-CVI for the full tool was within the acceptable range. The tool scored highly on all face validity measurements.This is the first validated CD tool designed specifically for use by multidisciplinary theatre teams which enables the debriefing to be flexibly tailored to the required intention. Future work should focus on maximizing successful implementation. Making the TTT widely available to facilitate CD could have significant benefits for theatre teams and their patients.

GEMINI GOOGLE: A POTENTIAL TOOL FOR ENGLISH LEARNING

Based on previous surveys, it is evident that Food Technology students at Thu Dau Mot University face challenges in learning English, such as limited vocabulary, weak grammar, inaccurate pronunciation, and poor listening and speaking skills. These can lead to declining academic performance and restricted career opportunities. The paper highlights the potential of Artificial Intelligence (AI) in overcoming these challenges. AI tools like Gemini Google can provide personalized learning experiences, improve interaction with the language, and offer immediate feedback. Gemini is specifically chosen due to its free access, Vietnamese interface, and support for over 40 languages. This study demonstrates that Gemini is an effective tool for undergraduate students, particularly those in Food Technology who have weak English backgrounds, to improve their English skills. Gemini improves vocabulary, grammar, speaking, listening, pronunciation, reading, and writing. It offers simple instructions and ideas in both English and Vietnamese, making it ideal for beginners, self-learners, and people with limited English proficiency. Unlike traditional learning, Gemini provides access at any time and from any location, as well as the ability to overcome shyness and blunders. Gemini's large, up-to-date database and user-friendly interface enable personalized learning paths and can be paired with other methods for best language learning. The study also proposes various strategies for utilizing Gemini Google to improve English learning effectiveness in areas like vocabulary, grammar, listening, speaking, reading, writing, and creating a positive learning environment. Furthermore, Gemini's value extends beyond languages, offering assistance in a variety of other areas

Empowering Patient Hand Hygiene and Reducing Infection in the Oncology Population

Background: Significant focus has been placed on healthcare worker hand hygiene, but little attention is has been assigned to the role of patient hand hygiene (HH) in reducing hospital acquired infections. Therefore, in this quality improvement study, we examined the impact of providing patients with hand hygiene products around mealtime on increasing patient HH adherence and on reducing acquisition of nosocomial antibiotic resistant organisms. Methods: Patients on two inpatient leukemia units at a tertiary oncologic center were provided with a single use pre-packaged alcohol wipe on their meal trays prior to every meal (three times daily). Additionally, an information card explaining to patients how and when to use the alcohol wipe was provided on the meal trays three times a week. Both the wipe and instructions were designed with input from patient representatives at the hospital. Two oncologic control units were selected where no specific intervention for patient hand hygiene was conducted. Patient hand hygiene adherence on the control and intervention units were measured through once monthly patient interviews conducted after meals where patients were asked to recall whether they washed their hands prior to eating (using any product). Vancomycin Resistant Enterococcus (VRE) incidence was compared on the intervention and control units during the 7 months prior and 7 months following initiating the intervention. Results: During the seven-month intervention period, more than 15 000 wipes were dispensed to patients on the intervention units. Through interview, 91% of 87 patients on the intervention units reported cleaning their hands before eating a meal using any cleaning product compared to 72% of 68 patients on the control units (X2 = 9.32, p = 0.002). Furthermore, on the intervention units, 30 (38%) patients endorsed using the provided hand hygiene product. During intervention period, the combined incidence rate of VRE the intervention units was 1.85 case/1000 patient-days compared to 5.35 cases/1000 patient-days during the 7 months prior to intervention (t = 3.24, p=0.007)(Figure 1). Conclusions: This patient-centered quality improvement intervention increased patient hand hygiene and potentially reduced VRE incidence in a vulnerable oncologic population. This practical intervention that incorporated the patient perspective provided accessible hand hygiene products with simple instruction and reminders required minimal participation of unit staff. Further application of the intervention in non-oncologic populations is needed to further establish the relationship between patient hand hygiene and the acquisition of nosocomial infections.

Centering and flourishing: an online intervention study assessing the effects of a Christian contemplative practice on stress-reduction and human flourishing

Despite widespread engagement in contemplative religious practices, comparatively little research has been conducted on their potential effects on well-being. Furthermore, few studies have focused on how an explicitly religious framing may impact the outcomes of such practices. In this online randomized controlled trial (N = 702), we tested the well-being effects of a contemplative prayer practice called Centering Prayer on self-identifying Christians. We compared 1) presenting the practice with an explicitly religious framing (experimental condition), 2) presenting the practice without an explicitly religious framing (active control), and 3) presenting simple instructions to reflect on the day, without any instructions regarding a meditation-like practice (passive control). After randomization into one of these three conditions, participants were asked to complete their assigned practice daily for 28 days. We hypothesized that the religious framing version of the practice would increase well-being over the active and passive control conditions. Well-being was assessed at three follow-up time points: one day, one week, and one month after the practice period. We found no group differences between the conditions on our primary outcome measure of well-being at one-week post-intervention. Each group increased in well-being from baseline to follow-up. We found significant group differences on acute measures of spiritual experience, the Mystical Experience Questionnaire (MEQ-30) and Daily Spiritual Experience Questionnaire (DSES). These results suggest that a religious framing may not enhance well-being effects but may alter spiritual outcomes related to contemplative practices.

Navigating cancer care through digital dialogue: Analyzing key phrases shared by patients in real-time exchange messages using an electronic patient-reported outcome measure (ePROM).

e13661 Background: Cancer is a complex ailment, giving rise to a myriad of symptoms, side effects, and questions that may arise unexpectedly. There is a growing interest in adopting more efficient approaches for the continuous monitoring of patients, particularly through virtual platforms. This study focuses on analyzing the word frequency in the context of cancer assistance using an ePROM. Methods: We conducted an analysis of an ePROM embedded in a mobile app designed for monitoring patients undergoing cancer treatment. Developed collaboratively by medical oncologists and software engineers, this app empowers patients to document their symptoms, quality of life, emotions, emergency room visits, and hospitalizations. Upon app registration, patients willingly share their data with their medical team, along with essential demographic information. A standout feature is the online chat facilitating real-time communication with the medical team, consolidating all information securely in one place. This patient-centric app is provided free of charge. Our study involved a retrospective and descriptive analysis focusing solely on data related to exchanged messages in the online chat between cancer patients and the medical team. These conversations spanned from February 2022 to January 2024 and included patients referred by physicians recommending our platform. The analysis centered on the frequency of non-technical words used in real-time conversations, clustered into semantic groups. Results: Our findings revealed a total exchange of 470,597 words across 684 conversations over a two-year period. In the healthcare context, the most frequent words were categorized as follows: 1) Seeking in-person assistance (568; 0.12%): hospital (424 mentions), emergency (82), urgent/urgency (51), and primary care (UBS, Portuguese acronym) (11); 2) Doubts regarding anything (979; 0.20%): doubt (694 mentions) and question (285); 3) Non-technical one-word symptoms (5213; 1.10%): pain (1821 mentions), nausea (961), tiredness (514), diarrhea (428), vomit (364), fever (304), cough (221), numbness (197), dizziness (162), insomnia (130), constipation (101), and wheezing (10). Conclusions: Our analysis showed two key insights: first, doubts persist throughout treatment, intertwined with the management of side effects; and second, the burden on patients is substantial, whether stemming from uncertainty about the next steps or the necessity for in-person assistance. Cancer's mental and physical toll underscores the importance of having a readily available team to provide guidance, even on simple instructions. In this light, virtual platforms may represent the future of care delivery. Continuous patient monitoring emerges as pivotal for enhancing healthcare assistance, treatment, and outcomes across various cancer types.

Instruction on the Scientific Method Provides (Some) Protection Against Illusions of Causality.

People tend to overestimate the efficacy of an ineffective treatment when they experience the treatment and its supposed outcome co-occurring frequently. This is referred to as the outcome density effect. Here, we attempted to improve the accuracy of participants' assessments of an ineffective treatment by instructing them about the scientific practice of comparing treatment effects against a relevant base-rate, i.e., when no treatment is delivered. The effect of these instructions was assessed in both a trial-by-trial contingency learning task, where cue administration was either decided by the participant (Experiments 1 & 2) or pre-determined by the experimenter (Experiment 3), as well as in summary format where all information was presented on a single screen (Experiment 4). Overall, we found two means by which base-rate instructions influence efficacy ratings for the ineffective treatment: 1) When information was presented sequentially, the benefit of base-rate instructions on illusory belief was mediated by reduced sampling of cue-present trials, and 2) When information was presented in summary format, we found a direct effect of base-rate instruction on reducing causal illusion. Together, these findings suggest that simple instructions on the scientific method were able to decrease participants' (over-)weighting of cue-outcome coincidences when making causal judgements, as well as decrease their tendency to over-sample cue-present events. However, the effect of base-rate instructions on correcting illusory beliefs was incomplete, and participants still showed illusory causal judgements when the probability of the outcome occurring was high. Thus, simple textual information about assessing causal relationships is partially effective in influencing people's judgements of treatment efficacy, suggesting an important role of scientific instruction in debiasing cognitive errors.

DEVELOPMENT OF AN APPLICATION AS AN AUXILIARY TOOL FOR VERIFICATION OF AN ELECTROCARDIOGRAM CONCLUSION

Objective: Electrocardiography (ECG) is a key step in the diagnosis of cardiovascular pathology. Interpretation of the ECG is carried out by the doctor in the first minutes of severe patient admission and often determines further tactics of management. However, such an important diagnostic method is quite subjective, since it is highly dependent on the knowledge, and experience of the doctor and even on his attention to detail. The objective is to develop an application for automatic verification of various ECG criteria (exceptionally as an addition to the subjective interpretation of the doctor). Design and method: The desktop application was developed in Python (v3.10.5) by implementing its object-oriented programming concepts. Tkinter was chosen as a Graphical User Interface framework. The set of standard libraries has been supplemented by OpenCV (v4.6.0.66) for recognizing and digitizing ECG images, Pillow (v9.3.0) for manipulating the image and its metadata, NumPy (v1.23.5) for further analysis, and others. The initial version of the program includes STEMI recognition. Real ECGs printed on graph paper were used for debugging and testing. Results: When launching the application, the user is prompted to select a file containing an ECG image (a). After the required image has opened, the user should indicate the requested ECG-leads in the picture, following the simplest instructions (b). Then processing of the leads automatically starts. This phase is hidden from the user, and only a progress bar is displayed on the screen (c). Meanwhile, a number of manipulations are performed, such as converting the picture to a binary state, recognizing the ECG-line, and storing the coordinates of each pixel in the database. Objects of such classes as Isoline, and Cardiac Cycle with R wave, ST segment, etc. are created for each of the objects of the Lead class (d). Further geometric study of the pixel base and checking for the pathological ECG-signs on the backend form the final conclusion for the user (e). Conclusions: The developed application is relevant for use as an additional tool in the work and training of doctors. It can be expanded to cover a wide range of pathologies.

PERSPECTIVES OF PEOPLE WITH CHRONIC KIDNEY DISEASE REGARDING DIGITAL HEALTH INTERVENTIONS THAT PROMOTE A HEALTHY LIFESTYLE: QUALITATIVE SYSTEMATIC REVIEW

Chronic kidney disease (CKD) is a life-limiting condition that affects 1 in 10 adults and accounts for 12% of all deaths in Australia. Diet and physical activity are crucial to attenuate disease progression and reduce mortality risk. Digital health interventions have been proposed as a feasible model to deliver lifestyle interventions. Users’ perspectives are key to ensure the intervention is aligned with their needs and goals. As such, this systematic review aimed to synthesise the perspectives of people with CKD and develop higher order concepts that elucidate users’ preferences regarding digital health interventions that promote heathy lifestyle. This was a qualitative systematic review. A database search was conducted on CENTRAL, Scopus, MEDLINE, CINAHL and SPORTDiscus between 2000-2023. Eligibility criteria included primary research that reported the preferences of adults with CKD regarding digital lifestyle interventions. Two independent reviewers completed title, abstract and full text screening. Quotes were extracted verbatim, coded and categorised. Categories were then used to generate themes. Database search identified 5,761 records. One additional record was included following communication with a primary author. Fifteen papers were eligible, including 197 participants (age 51.0±7.2 years; 47% female). There were 83 people with CKD 1-5; 61 transplant recipients; and 53 people receiving maintenance dialysis. The overarching theme identified was that people with CKD considered digital interventions to be important platforms to access lifestyle interventions. There were five underlying themes that elucidated users’ preferences for digital interventions, including simple instruction and engaging design; individualised interventions; virtual communities of care; education and action plans; and timely reminders and automated behavioural monitoring. Digital health interventions were considered important platforms to deliver lifestyle interventions. Future research may apply users’ preferences in this review to inform the development and conduct of a digital lifestyle intervention for people with CKD.

Preferences of people with chronic kidney disease regarding digital health interventions that promote healthy lifestyle: qualitative systematic review with meta-ethnography

ObjectivesDiet and physical activity are crucial for people with chronic kidney disease (CKD) to maintain good health. Digital health interventions can increase access to lifestyle services. However, consumers’ perspectives are...

Le dossier Humani generis du Saint-Office

The Holy Office’s file on the drafting of the encyclical Humani generis allows to understand how a simple instruction against French Jesuits’ theological tendencies, became a text of universal scope. In the course of its five successive versions, and following the indications of the Pope, the text in preparation thus gave birth to the Humani generis encyclical, naming no authors and finally published without the syllabus of erroneous propositions which had already been prepared. Jesuits who were close to the Pope or to the Superior General Janssens played a decisive role in its drafting, along with the Italian prelate Mgr Parente.

32 HANDFULS: A New Clinically Feasible Measure of Hand Outcome for Children with Burns and Trauma

Abstract Introduction Fine motor dexterity is important for children's normal hand function and development. In-hand manipulation (the collection and repositioning of items in the hand) and palmar workspace (functional volume in the palm) are two aspects of hand function that often become simultaneously impaired after hand trauma or burn injury. Rehabilitation emphasizes recovery of these hand functions yet few functional outcome measures address both of these constructs and even fewer are appropriate for children. Methods This study developed a new hand outcome measure to specifically assess these constructs and tested it on 192 children between the ages of 2-21. The test, called ‘HANDFULs’, uses simple instructions and inexpensive materials (i.e. marbles) shown in Figure 1. Means, standard deviations, and 95% confidence limits were estimated for children in seven age groups. Associations between age and the HANDFULs variables were examined using one-factor ANOVA. Multiple regressions were fit to test for associations between age and the HANDFULs variables after controlling for height, hand size, and hand strength. A stepwise selection procedure was then implemented, holding age into the model, to remove otherwise non-significant variables. Test-retest reliability was tested with the Shrout Fleiss Reliability Index and significance was set at p< 0.05. Results Expected normal values are reported in Figure 2 for palmar workspace volume (marbles that fit in the hand) and in-hand manipulation (collection time per marble) by age group. Age (0.16 (0.06), p=0.009), hand span (0.47 (0.09), p< 0.0001) and hand width (0.9 (0.29), p=0.003) were significantly associated with number of marbles that fit in the hand, representing palmar workspace capacity. Although hand strength and size characteristics were tested, only age (-0.02 (0.01), p=0.001) was significantly associated with the HANDFULs score of time per marble. The Intraclass Correlation Coefficient was 0.94 demonstrating excellent test-retest reliability. Conclusions This study developed a new pediatric hand outcome measure to address hand function to address a gap related to burn and trauma recovery. The measure was tested on able-bodied, noninjured children to establish validity and reliability, provide reference values for patients with injuries by age groups, and is clinically feasible to administer. Applicability of Research to Practice Outcome test for burn injured hands.

Structuring medication signeturs as a language regression task: comparison of zero- and few-shot GPT with fine-tuned models.

Electronic health record textual sources such as medication signeturs (sigs) contain valuable information that is not always available in structured form. Commonly processed through manual annotation, this repetitive and time-consuming task could be fully automated using large language models (LLMs). While most sigs include simple instructions, some include complex patterns. We aimed to compare the performance of GPT-3.5 and GPT-4 with smaller fine-tuned models (ClinicalBERT, BlueBERT) in extracting the average daily dose of 2 immunomodulating medications with frequent complex sigs: hydroxychloroquine, and prednisone. Using manually annotated sigs as the gold standard, we compared the performance of these models in 702 hydroxychloroquine and 22104 prednisone prescriptions. GPT-4 vastly outperformed all other models for this task at any level of in-context learning. With 100 in-context examples, the model correctly annotates 94% of hydroxychloroquine and 95% of prednisone sigs to within 1 significant digit. Error analysis conducted by 2 additional manual annotators on annotator-model disagreements suggests that the vast majority of disagreements are model errors. Many model errors relate to ambiguous sigs on which there was also frequent annotator disagreement. Paired with minimal manual annotation, GPT-4 achieved excellent performance for language regression of complex medication sigs and vastly outperforms GPT-3.5, ClinicalBERT, and BlueBERT. However, the number of in-context examples needed to reach maximum performance was similar to GPT-3.5. LLMs show great potential to rapidly extract structured data from sigs in no-code fashion for clinical and research applications.

Feel Your Best Self: Insights from elementary teachers’ use in teaching emotion-focused coping strategies

Feel Your Best Self (FYBS) teaches 12 emotion-focused coping strategies to strengthen emotion regulation and day-to-day experiences of positive emotions. Released in 2022, FYBS was designed to be implemented flexibly and with minimal preparation by elementary educators. In this manuscript, we present findings from a case study of early adopters (n = 6), sharing insights into how they have used, adapted, and generalized the program with their students. Participants ranged from first to fifth grade teachers. Four teachers implemented in English and two teachers implemented in Spanish. Findings suggest that early adopters “kept it simple;” leaned into opportunities to promote joy, engagement, and access using FYBS; adjusted instructional activities for developmental appropriateness; and integrated additional best practices. These insights suggest that FYBS is achieving its intended goal of simple and flexible instruction of emotion-focused coping strategies in elementary classrooms. We conclude by discussing next steps in expanding FYBS materials and testing the program’s cultural responsiveness and impact on student outcomes.

Multi-Intent Inline Code Comment Generation via Large Language Model

Code comment generation typically refers to the process of generating concise natural language descriptions for a piece of code, which facilitates program comprehension activities. Inline code comments, as a part of code comments, are also crucial for program comprehension. Recently, the emergence of large language models (LLMs) has significantly boosted the performance of natural language processing tasks. This naturally inspires us to explore the performance of the LLMs in the task of inline code comment generation. To this end, we evaluate open-source LLMs on a large-scale dataset and compare the results with the current state-of-the-art methods. Specifically, we explore the model performance in the following scenarios based on the widely used evaluation metrics (i.e. BLEU, Meteor, and ROUGE-L): (1) generation with simple instruction; (2) few-shot-guided generation with random examples selected from the database; (3) few-shot-guided generation with similar examples selected from the database; and (4) adopt the re-ranking strategy for the output of LLMs. Our findings reveal that: (1) under the simple instruction scenario, LLMs could not fully show the potential in the task of inline comment generation compared to the state-of-the-art models; (2) random few-shot leads to a slight improvement; (3) similar few-shot and re-ranking strategy could significantly enhance the performance of LLMs; and (4) for inline comment and code snippet pairs with different intents, why category achieves the best performance and what category achieves relatively poorer performance. That remains consistent across all four scenarios. Our findings shed light on future research directions for using LLMs in inline comment generation tasks.

Proses Terapi Sensori Integrasi Pada Anak Autis Dalam Meningkatkan Interaksi Sosial Di Pusat Terapi Autis Yasmin

Sensory Integration Therapy is an approach that focuses on sensory stimulation to improve sensory processing and adaptive responses in individuals with sensory disorders, one of which is children with autism. Sensory integration therapy makes it easier for autistic children to communicate to be better understood and to create good social interactions. Therefore, the Yasmin Autism Therapy Center carries out Sensory Integration Therapy to improve social interaction in autistic children. This research aims to determine the application of sensory integration therapy at the Yasmin Autism Therapy Center to improve social interaction in autistic children and determine the obstacles in implementing sensory integration therapy. Data collection techniques include documentation, observation and interviews. The results of the research show that the stages of Sensory Integration Therapy at the Yasmin Autism Therapy Center are providing simple instructions, a series of sequential activities and honing concentration, consistency and endurance in activities through play. Supporting factors for sensory therapy activities include good collaboration between therapists and parents. Meanwhile, the obstacles to children's communication are difficult factors, limited therapy tools, and limited therapy time.

1897 Older stroke survivors and rehabilitation therapists’ views on home-based resistance exercise for upper limbs

Abstract Introduction Upper limb recovery after stroke depends on participating in personalised task-specific exercise programmes. However, older adults with stroke find it challenging to maintain an optimal level of engagement with such exercise programmes due to personal and environmental factors. The aim of this study was to explore the views of stroke survivors and rehabilitation therapists on home-based resistance exercises for upper limbs. Method A qualitative study of semi-structured virtual and in-person interviews was conducted between January and March 2023 in England. Participants were 11 older adults >65 years (6 females (55%), with chronic stroke (>1 year after a stroke) and moderate to severe disabilities. Twenty rehabilitation therapists were also interviewed (3 physiotherapists, and 17 Action for Rehabilitation from Neurological Injury (ARNI) instructors). The group was asked about perceived personal and environmental barriers and facilitators as well as expectations of resistance exercises in relation to upper limb neurorehabilitation programmes. Interview sessions were audio recorded for transcription and thematic data analysis. The study was approved by Sheffield Hallam University ethics committee (reference number: AA43961583). Results Both groups mentioned that the main barrier to performing upper-limb exercises is weakness in the paretic arm affecting grip strength with consequent adverse impact on adherence to a home-based programme and poor patient motivation. Patients also reported safety concerns such as dropping equipment and being dependent on a carer to undertake regular exercises. Stroke survivors preferred a program that activates the paretic arm and is relevant to their daily functional activities. They preferred simple exercise instructions and demonstrations through visual aids and video materials. Patients also valued regular feedback on adjusting their exercise dose, monitoring progress over time, and ongoing encouragement. Conclusions Our study showed that designing home-based resistance exercises for upper limb in older stroke survivors should be individualised, functionally orientated, and motivational.

МЕТОДОЛОГИЯ PIEF ПО ПОВЫШЕНИЮ ЭФФЕКТИВНОСТИ БИЗНЕС-ПРОЦЕССОВ И ИХ АВТОМАТИЗАЦИИ В МЕЖДУНАРОДНОЙ ОПТОВОЙ ТОРГОВЛЕ

The article is devoted to the study of modern approaches to increasing the efficiency of business processes and their automation in a company. As an example, the Precise Instructions For Employee To Follow methodology, compiled in the practical activities of the author of this study, is given - “precise instructions that an employee must follow,” or PIEF. The views of modern authors on solving problems of increasing the efficiency of business processes and their automation are systematized. The necessity of organizing labor processes (labor) in a company is substantiated as a primary factor in increasing the efficiency of business processes. The significant role of precise, simple and accessible instructions in the quality organization of personnel work is highlighted. The potential benefits are analyzed, including: reduction of losses and downtime due to clear regulation of actions; accessibility of the activity and its phased nature; maintaining the creative functions of employees; focus on a specific goal. The effectiveness of applying the author's methodology in the tasks of the international wholesale trade business is emphasized, since PIEF allows you to implement with high efficiency the entire range of typical tasks that arise in the activities of a wholesale trade enterprise.

Development and evaluation of two rapid lateral flow assays for on-site detection of African swine fever virus.

African swine fever (ASF) is a lethal and highly contagious transboundary animal disease with the potential for rapid international spread. In the absence of a widely available and definitively proven vaccine, rapid and early detection is critical for ASF control. The quick and user-friendly lateral flow assay (LFA) can easily be performed by following simple instructions and is ideal for on-site use. This study describes the development and validation of two LFAs for the rapid detection of ASF virus (ASFV) in pig serum. The highly immunogenic antigens (p30 and p72) of ASFV Georgia 2007/1 (genotype II) were expressed in plants (Nicotiana benthamiana) and were used to immunize BALB/c mice to generate specific monoclonal antibodies (mAbs) against the p30 and p72 proteins. mAbs with the strongest binding ability to each protein were used to develop p30_LFA and p72_LFA for detecting the respective ASFV antigens. The assays were first evaluated using a spike-in test by adding the purified p30 or p72 protein to a serum sample from a healthy donor pig. Further validation of the tests was carried out using serum samples derived from experimentally infected domestic pigs, field domestic pigs, and feral pigs, and the results were compared with those of ASFV real-time PCR. p30_LFA and p72_LFA showed no cross-reaction with common swine viruses and delivered visual results in 15 min. When testing with serially diluted proteins in swine serum samples, analytical sensitivity reached 10 ng/test for p30_LFA and 20 ng/test for p72_LFA. Using real-time PCR as a reference, both assays demonstrated high sensitivity (84.21% for p30_LFA and 100% for p72_LFA) with experimentally ASFV-infected pig sera. Specificity was 100% for both LFAs using a panel of PBS-inoculated domestic pig sera. Excellent specificity was also shown for field domestic pig sera (100% for p30_LFA and 93% for p72_LFA) and feral pig sera (100% for both LFAs). The results obtained in this study suggest that p30_LFA and p72_LFA hold promise as rapid, sensitive, user-friendly, and field-deployable tools for ASF control, particularly in settings with limited laboratory resources.