This paper proposed a new formula for predicting acoustic power transmission loss of simple expansion chamber with two internal partitions. Seventeen cases of computational results were performed and the database was constructed for the TL according to the various positions of internal partitions. Using this database, firstly, the formula for the peak values of TL at certain frequencies was developed using the least square estimation. Secondly, the formula for the TL curve could be obtained automatically with the input data of the positions of two internal partitions. The formula of TL developed in this paper showed good agreement with computational results. This formula will be helpful for the positioning of internal partitions to improve TL at target frequencies.