Simple Changes Research Articles

Experimental observation of mesoscopic fluctuations to identify origin of thermodynamic anomalies of ambient liquid water

We report a new experimental approach for observing mesoscopic fluctuations underlying the thermodynamic anomalies of ambient liquid water. In this approach, two sound velocity measurements with different frequencies, namely inelastic X-ray scattering (IXS) in THz band and ultrasonic (US) in MHz band, are required to investigate the relaxation phenomenon with the characteristic frequency between the two aforementioned frequencies. We performed IXS measurements to obtain the IXS sound velocity of liquid water from the ambient conditions to the supercritical region of liquid-gas phase transition (LGT) and compared the results with the US sound velocity in the literature. We found that the ratio of the two sound velocities, Sf, which corresponds to the relaxation intensity, exhibits a simple but significant change. Two distinct rises were observed in the high-temperature and low-temperature regions, implying that two relaxation phenomena exist: in the high-temperature region, a peak was observed near the LGT critical ridge line, which was linked with changes in the density fluctuation and isochoric and isobaric specific heat capacities; in the low-temperature region, Sf increased toward the low-temperature region, which was linked with the change in the isochoric heat capacity. We concluded that these two relaxation phenomena are originated from critical fluctuations of liquid-gas phase transition (LGT) and liquid-liquid phase transition, respectively. The linkage between Sf and isochoric heat capacity in the low-temperature region proves that the relaxation is the cause of the well-known heat capacity anomaly of ambient liquid water. In this study, both LGT and LLT critical fluctuations were observed, and the relationship between thermodynamics and the critical fluctuations was comprehensively discussed.

Starry Nights Are Disappearing

Stars dim as the sky gets brighter, a result of expanding cities and bright LEDs. Simple low-tech changes can help preserve dark night skies.

A half‐century check‐up for child neurology training

A half‐century check‐up for child neurology training

A life cycle assessment of guar agriculture

Guar gum, the main product of the guar crop, is used widely in the USA as an emulsifier in the food industry and as fracturing fluid additive in the oil and gas industry. The USA is the number one global importer of guar, and interest has grown to domestically cultivate guar in the USA. Guar is an annual desert legume native to India and Pakistan. The goal of this study was to evaluate the environmental sustainability of growing guar in the USA via a life cycle analysis (LCA). The LCA helps identify the information gap for US agriculture and guide future field studies to optimize guar cultivation in the USA. This study concluded that in terms of environmental sustainability, irrigation, harvesting, and P-fertilization methods offer the most opportunity for improved guar agricultural sustainability. This is promising because one of guar’s prominent characteristics is its high water use efficiency and ability to grow in marginal soils. Lowering irrigation and water use can be implemented with simple management practice changes such as optimizing irrigation. In addition, this study shows that there is an opportunity for field trials to optimize fertilizer application rates to achieve the greatest yields. This study also found a knowledge gap with respect to carbon soil fluxes and field emissions of nitrogen and phosphorus from guar agriculture. As the USA considers adopting guar agriculture in the southwest, through research collaborations such as the USDA Sustainable Bioeconomy of Arid Regions Center of Excellence, it will be critical to evaluate irrigation to achieve maximum yields (e.g., drip, flood, sprinkler) and fill fertilizer and emissions knowledge gaps.

Anti-CD44 antibodies grafted immunoaffinity Fe3O4@MnO2 nanozymes with highly oxidase-like catalytic activity for specific detection of triple-negative breast cancer MDA-MB-231 cells

Cell-enzyme-linked immunosorbent assay (CELISA) is extensively applied for cancer diagnosis and screening because of its simple operation, high sensitivity, and intuitive color change. However, the unstable horseradish peroxidase (HRP), hydrogen peroxide (H2O2) and non-specificity have led to a high false negative rate, which limits its application. In this study, we have developed an innovative immunoaffinity nanozyme aided CELISA based on anti-CD44 monoclonal antibodies (mAbs) bioconjugated manganese dioxide-modified magnetite nanoparticles (Fe3O4@MnO2 NPs) for the specific detection of triple-negative breast cancer MDA-MB-231 cells. The CD44FM nanozymes were fabricated to replace unstable HRP and H2O2 to counteract possible negative effects in conventional CELISA. Results suggested that CD44FM nanozymes displayed remarkable oxidase-like activities over an extensive pH and temperature range. The bioconjugation of CD44 mAbs enabled CD44FM nanozymes to enter MDA-MB-231 cells selectively via over-expressed CD44 antigens on the membrane surface of these cells, and then catalyzed oxidation of the chromogenic substrate TMB, further achieving specific detection of these cells. Additionally, this study exhibited high sensitivity and low detection limit for MDA-MB-231 cells with a quantitation range of just 186 cells. To sum up, this report developed a simple, specific and sensitive assay platform based on CD44FM nanozymes, which could provide a promising strategy for targeted diagnosis and screening of breast cancer.

Abstract TP31: Achieving Compliance With A Nurse Driven Stroke Timer Initiative

Background: The concept “time is brain” has guided the international stroke community since the early nineties. The use of a visual countdown has been shown to improve turnaround times during acute stroke management; however, use of these devices is not a standard of practice and therefore not used consistently in most hospitals. Furthermore, compliance with a stroke timer initiative can be hampered by logistical and emotional responses to the timer concept. Purpose: The purpose of this project is to improve compliance with a stroke countdown timer during stroke alerts for potential acute ischemic stroke reperfusion treatments (thrombolysis and/or neuroendovascular intervention) candidates at our Joint Commission CSC. Methodology: A pre- and post-intervention design method was used by implementing measures to improve timer adherence. These included changing the timer storage location and modification to the nurse stroke alert documentation to capture timer utilization. Stoke nurse education about these changes and the 100% compliance expectation was provided. The countdown timer was set to 25-min from arrival for IV thrombolysis, then reset to 35 minutes for puncture access for mechanical thrombectomy. To minimize stress on the stroke response team, there were no alarms or push buttons necessary, other than the one needed to set the countdown timer upon patient arrival. Results: During the study period, the median DTN, DTP, DTD were 21, 69, and 81 minutes respectively. After post-intervention implementation, the stroke clock was utilized in 92% (n=58) of the stroke alerts, a 50 percent increase from initial utilization (42%, n=31). There was no statistical significance found in any of the treatment times (H[2]=0.280, p=.869 [DTN]; H[2]=2.238, p=.327 [DTP]; H[2]=1.973, p=0.373 [DTD]) despite a median decrease of 15 minutes on DTP and DTD with improved compliance. Conclusions: Great compliance with a nurse driven stroke countdown timer initiative can be achieved with simple workflow changes however it did not result in improved treatment performance metrics. Our analysis was limited by a small sample size. We are planning on a larger analysis using data from our multicenter stroke network.

Combined Analysis of Net Groundwater Recharge Using Water Budget and Climate Change Scenarios

Estimating the groundwater recharge rate is essential in all groundwater-related fields, including groundwater development, use, management, modeling, and contamination analysis. In this study, we proposed a combined method of water budget and climate change scenario for estimating the net groundwater recharge rate in the Nakdong River watershed (NRW), South Korea. For the climate change scenario method, the representative concentration pathway (RCP) 4.5 and 8.5 climate scenarios were adopted. First, using the water budget method from 2009 to 2018, the net groundwater recharge rate (NGRR) of 12.15–18.10% relative to annual precipitation (AP) was obtained, subtracting direct runoff (DR) of 21.18–25.32% relative to AP, evapotranspiration (EP) of 40.53–52.29% relative to AP, and baseflow of 12.42–17.84% relative to AP, from the AP (865–1494 mm). The average annual NGRR of the NRW was 200 mm (15.59%). Second, the mean NGRRs from 2009 to 2100 under the RCP 4.5 and RCP 8.5 scenarios were anticipated as 8.73% and 7.63%, respectively. The similarity between the water budget and climate change scenarios was confirmed using data from 2009 and 2018. According to the simple climate change scenario, it is predicted that annual precipitation will increase over the years while the groundwater level and net groundwater recharge rate will decrease. Nonetheless, the estimated NGRR by the water budget method in this study possesses uncertainty due to using potential ET instead of actual ET which should be estimated by considering soil water content.

New Nostocyclophanes from Nostoc linckia.

Six new nostocyclophanes and four known compounds have been isolated from Nostoc linckia (Nostocaceae) cyanobacterial strain UTEX B1932. The new compounds, nostocyclophanes E-J (1-6), were characterized by NMR and MS techniques. The known compounds were nostocyclophanes B-D, previously isolated from this strain, and dedichloronostocyclophane D. Structural modifications on the new [7.7]paracyclophane analogs 1-5, isolated from the 80% methanol fraction, range from simple changes such as the lack of methylation or halogenation to more unusual modifications such as those seen in nostocyclophane H (4), in which the exocyclic alkyl chains are of different length; this is the first time this modification has been observed in this family of natural products. In addition, nostocyclophane J (6) is a linear analog in which C-20 is chlorinated in preparation for the presumed enzymatic Friedel-Craft cyclization needed to form the final ring structure, analogous to the biosynthesis of the related cylindrocyclophanes. Nostocyclophane D, dedichloronostocyclophane D, and nostocyclophanes E-J demonstrated moderate to weak growth inhibition against MDA-MB-231 breast cancer cells.

Altered binaural hearing in pre-ataxic and ataxic mutation carriers of spinocerebellar ataxia type 3

Brainstem degeneration is a prominent feature of spinocerebellar ataxia type 3 (SCA3), involving structures that execute binaural synchronization with microsecond precision. As a consequence, auditory processing may deteriorate during the course of disease. We tested whether the binaural “Huggins pitch” effect is suitable to study the temporal precision of brainstem functioning in SCA3 mutation carriers. We expected that they would have difficulties perceiving Huggins pitch at high frequencies, and that they would show attenuated neuromagnetic responses to Huggins pitch. The upper limit of Huggins pitch perception was psychoacoustically determined in 18 pre-ataxic and ataxic SCA3 mutation carriers and in 18 age-matched healthy controls. Moreover, the cortical N100 response following Huggins pitch onset was acquired by means of magnetoencephalography (MEG). MEG recordings were analyzed using dipole source modeling and comprised a monaural pitch condition and a no-pitch condition with simple binaural correlation changes. Compared with age-matched controls, ataxic but not pre-ataxic SCA3 mutation carriers had significantly lower frequency limits up to which Huggins pitch could be heard. Listeners with lower frequency limits also showed diminished MEG responses to Huggins pitch, but not in the two control conditions. Huggins pitch is a promising tool to assess brainstem functioning in ataxic SCA3 patients. Future studies should refine the psychophysiological setup to capture possible performance decrements also in pre-ataxic mutation carriers. Longitudinal observations will be needed to prove the potential of the assessment of Huggins pitch as a biomarker to track brainstem functioning during the disease course in SCA3.

Aging Effects on Optic Nerve Neurodegeneration.

Common risk factors for many ocular pathologies involve non-pathologic, age-related damage to the optic nerve. Understanding the mechanisms of age-related changes can facilitate targeted treatments for ocular pathologies that arise at any point in life. In this review, we examine these age-related, neurodegenerative changes in the optic nerve, contextualize these changes from the anatomic to the molecular level, and appreciate their relationship with ocular pathophysiology. From simple structural and mechanical changes at the optic nerve head (ONH), to epigenetic and biochemical alterations of tissue and the environment, multiple age-dependent mechanisms drive extracellular matrix (ECM) remodeling, retinal ganglion cell (RGC) loss, and lowered regenerative ability of respective axons. In conjunction, aging decreases the ability of myelin to preserve maximal conductivity, even with "successfully" regenerated axons. Glial cells, however, regeneratively overcompensate and result in a microenvironment that promotes RGC axonal death. Better elucidating optic nerve neurodegeneration remains of interest, specifically investigating human ECM, RGCs, axons, oligodendrocytes, and astrocytes; clarifying the exact processes of aged ocular connective tissue alterations and their ultrastructural impacts; and developing novel technologies and pharmacotherapies that target known genetic, biochemical, matrisome, and neuroinflammatory markers. Management models should account for age-related changes when addressing glaucoma, diabetic retinopathy, and other blinding diseases.

Topical Anesthetic for Laceration Repair in Children.

The objective of this study is to evaluate the impact of the introduction of an education program familiarizing staff with the effective and appropriate use of Laceraine application to children's lacerations requiring repair at The Prince Charles Hospital Children's Emergency Department (TPCH-CED), Brisbane. A retrospective audit was performed using data obtained from the local emergency department information system (EDIS) and a paper chart audit, for all presentations to TPCH-CED during 2015 and 2020 requiring laceration repair. Of the 20,813 registered patients to TPCH-CED in 2015, 993 (4.8%) required laceration repair, compared with 1756 (5.6%) of the 31,059 registered in 2020 demonstrating that presentations to the CED requiring laceration repair increased, as a proportion of overall presentations, reaching statistical significance across all groups ( P < 0.001). The percentage of lacerations requiring repair that had Laceraine applied (either as a single agent or in combination) was 59% in 2015 and 93% in 2020 ( P < 0.001). There was a significant increase in use of Laceraine only as agent of choice. There was a significant change in practice with greater use of topical anesthetic (Laceraine) for the management of laceration repair of children, as expected, after the focused education program on its correct use. As emergency departments continue to be overwhelmed with increasing attendances and long patient wait times, a simple small intervention, such as that described, has potential to improve flow through the children's emergency department. Future research should focus on a randomized control trial to determine the contribution of the use of a topical agent, as opposed to injectable local anesthetic and/or full procedural sedation to determine the contribution to the impact of this simple change of practice on patient flow and satisfaction.

Identification of Alternative Splicing in Proteomes of Human Melanoma Cell Lines without RNA Sequencing Data.

Alternative splicing is one of the main regulation pathways in living cells beyond simple changes in the level of protein expression. Most of the approaches proposed in proteomics for the identification of specific splicing isoforms require a preliminary deep transcriptomic analysis of the sample under study, which is not always available, especially in the case of the re-analysis of previously acquired data. Herein, we developed new algorithms for the identification and validation of protein splice isoforms in proteomic data in the absence of RNA sequencing of the samples under study. The bioinformatic approaches were tested on the results of proteome analysis of human melanoma cell lines, obtained earlier by high-resolution liquid chromatography and mass spectrometry (LC-MS). A search for alternative splicing events for each of the cell lines studied was performed against the database generated from all known transcripts (RefSeq) and the one composed of peptide sequences, which included all biologically possible combinations of exons. The identifications were filtered using the prediction of both retention times and relative intensities of fragment ions in the corresponding mass spectra. The fragmentation mass spectra corresponding to the discovered alternative splicing events were additionally examined for artifacts. Selected splicing events were further validated at the mRNA level by quantitative PCR.

Stability and bifurcation analysis of a size-stage-structured cooperation model

In this paper, we propose a size-stage-structured cooperation model which has two distinct life stages in facultative cooperator. The primary feature of this model is to consider size structure, stage structure and obligate and facultative symbiosis at the same time in a cooperation system. We use the method of characteristic to show that this new model can be reduced to a threshold delay equations (TDEs) model, which can be further transformed into a functional differential equations (FDEs) model by a simple change of variables. Such simplification allows us to apply the classical theory of FDEs and establish a set of sufficient conditions to investigate the qualitative analysis of solutions of the FDEs model, including the global existence and uniqueness, positivity and boundedness. What’s more, we use the geometric criteria to get the conclusions about stability and Hopf bifurcation of positive equilibrium because the coefficients of the characteristic equation depend on the bifurcation parameter. Finally, numerical simulations are carried out as supporting evidences of our analytical results. Our results show that the presence of size structure and stage structure plays an important role in the dynamic behavior of the model.

Construction of Aluminum‐Porphyrin‐Based Hypercrosslinked Ionic Polymers (HIPs) by Direct Knitting Approach for CO2 Capture and In‐Situ Conversion to Cyclic Carbonates

AbstractHypercrosslinked polymers (HCPs) constructed by Friedel‐Crafts reaction have drawn increasing attention in recent decades, but their multifunctionalization remains a huge challenge. Herein, a series of aluminum‐porphyrin‐based hypercrosslinked ionic polymers have been successfully synthesized by direct knitting approach without additional electrophilic comonomers, which involves the copolymerization of neutral porphyrin monomers and ionic building. By increasing the connected nodes numbers of ionic monomers, the introduction of abundant tetraphenylmethane fragements into the porphyrin backbones endows them with high‐density ionic active sites. Accordingly, the well‐matched molar ratios of aluminum sites to nucleophilic chloride anions that work synergistically for CO2/epoxide coupling can be achieved. Al‐HIP‐2 with flexible ionic pendants and a high Al/Cl ratio allows CO2 cycloaddition to be performed at ambient conditions or with diluted CO2; while Al‐HIP‐3 with rigid ionic moieties and a relatively low Al/Cl ratio exhibits higher specific surface area and stronger CO2 capture ability. Therefore, this co‐condensation strategy provides a simple and feasible pathway for adjusting the reactivity ratios of comonomers via simple structural changes in the knitting process, thus achieving the rational construction of multifunctionalized HCPs for CO2 capture and in‐situ conversion to cyclic carbonates.

Typical large graphs with given edge and triangle densities

The analysis of large simple graphs with extreme values of the densities of edges and triangles has been extended to the statistical structure of typical graphs of fixed intermediate densities, by the use of large deviations of Erdős-Rényi graphs. We prove that the typical graph exhibits sharp singularities as the constraining densities vary between different curves of extreme values, and we determine the precise nature of the singularities. The extension to graphs with fixed densities of edges and k-cycles for odd \(k>3\) is straightforward and we note the simple changes in the proof.

G-quadruplex from precursor miR-1587 modulated its maturation and function

A certain proportion of pre-miRNAs, which contained potential G-quadruplex forming sequences, was found to act as a mediator to Dicer-mediated cleavage, and that the regulation of miRNA production and function may be achieved through the G-quadruplex structure. In this study, human precursor miR-1587 sequence was transfected after the incubation with different solution conditions (K+, TMPyP4, etc.). Firstly, the formation of G-quadruplex from precursor miR-1587 sequences was confirmed by CD and UV melting. The expression of miR-1587 level was then evaluated by Q-RT-PCR, and the results showed that the formation of G-quadruplex inhibited the miR-1587 maturation process, resulting in a reduced miR-1587 expression. Meanwhile the destabilization of G-quadruplex led to an increased miR-1587 expression by contrast. Then, the weakened inhibition of miR-1587 towards its target genes, such as TAGLN or NCOR1, was presented confirming by Q-RT-PCR and western blot. Molecular mechanism by dual-luciferase assays showed that the modulations of miR-1587 expression and function were due to the G-quadruplex structure transformation, but not the simple change of solution conditions. This study highlighted the importance of maintaining specific structures during miRNA biosynthesis and provided a way to alter the function of G-rich precursor miRNAs by modulating molecular conformation using ionic solutions or ligands.

Can a simple plan change a complex behavior? Implementation intentions in the context of water drinking

Underhydration has significant adverse physical and mental health effects, yet many people drink too little water. Implementation intentions have been found to effectively promote many health behaviors, but little is known about the processes underlying their effects in naturalistic settings, and whether they could improve water drinking. This mixed-methods study assessed the impact and potential underlying processes of using implementation intentions to increase self-reported water intake over a five-day follow-up. Ninety-five participants (Mage = 39, SD = 12) received an educational quiz to increase their water drinking motivation before being randomly assigned to the control or intervention group. Participants also completed a qualitative survey that assessed the processes underlying their attempts to increase water intake. Quantitative results suggested that most participants increased their average daily water intake regardless of group. Qualitative results indicated that implementation intention participants struggled with remembering and the perceived effort of preparation and drinking behaviors, which reduced the effect of planning on behavior. This study provides essential theoretical and methodological considerations for researchers studying implementation intentions, as the effects and mechanisms of implementation intentions in real-life situations may be more complex than previously assumed. For example, the results suggest that implementation intentions did not automatize remembering and performing the behavior in ways the current literature theorizes. Other kinds of interventions may be needed to improve the complex daily-life behaviour of water drinking.

On the Nekrasov partition function of gauged Argyres-Douglas theories

We study SU(2) gauge theories coupled to (A1, DN) theories with or without a fundamental hypermultiplet. For even N, a formula for the contribution of (A1, DN) to the Nekrasov partition function was recently obtained by us with Y. Sugawara and T. Uetoko. In this paper, we generalize it to the case of odd N in the classical limit, under the condition that the relevant couplings and vacuum expectation values of Coulomb branch operators of (A1, DN) are all turned off. We apply our formula to the (A2, A5) theory to find that its prepotential is related to that of the SU(2) gauge theory with four fundamental flavors by a simple change of variables.

CREATING MULTITHREADED PTOGRAMS IN C++

For many years, the increase in computing power of modern devices is achieved not by increasing the clock frequency and bandwidth of CPUs, but by using hyper-threaded and multicore architectures. This simple change in approach to the CPU design led to dramatic changes in the organization of computing and became a turning point for software developers. Software that is going to take advantage of the increased computing power of multi-core architectures must be designed to be able to perform multiple tasks simultaneously. When covering the topic of parallel/simultaneous computing, scientists and IT professionals use two terms: (1) parallelism and (2) concurrency. A third important term when considering parallel/simultaneous computing is “multithreading”. In C++, the two most common ways to implement parallelism are concurrency and parallelism itself. Although they can be used in other programming languages, C++ stands out for its ability to use concurrent computations with lower-than-average utilization of general machine resources, as well as its ability to execute complex instructions. The C++11 standard introduced support for multi-threaded programs. The C++ standard recognized the existence of multithreading in the language and provided components for writing multithreaded programs in the &lt;thread&gt; library. This made it possible to write multithreaded programs in C++ without relying on platform-specific extensions and enabled the creation of portable multithreaded code with guaranteed behavior. The &lt;thread&gt; library provides extensive opportunities for organizing parallel and concurrent calculations, it also contains the C++ memory model, conditional variables, mutexes, etc. for thread synchronization. The article analyzes the possibilities of writing programs in the C++ using multiple threads for parallel and concurrent execution of tasks. It also considers the features of the C++ and the tools of the &lt;thread&gt; library that make parallelism and concurrency possible.

Chromatin-RNA in situ Reverse Transcription Sequencing (CRIST-seq) Approach to Profile the Non-coding RNA Interaction Network.

Non-coding RNAs (ncRNAs) are defined as RNAs that do not encode proteins, but many ncRNAs do have the ability to regulate gene expression. These ncRNAs play a critical role in the epigenetic regulation of various physiological and pathological processes through diverse biochemical mechanisms. However, the existing screening methods to identify regulatory ncRNAs only focus on whole-cell expression levels and do not capture every ncRNA that targets certain genes. We describe a new method, chromatin-RNA in situ reverse transcription sequencing (CRIST-seq), that can identify all the ncRNAs that are associated with the regulation of any given gene. In this article, we targeted the ncRNAs that are associated with pluripotent gene Sox2, allowing us to catalog the ncRNA regulation network of pluripotency maintenance. This methodology is universally applicable for the study of epigenetic regulation of any genes by making simple changes on the CRISPR-dCas9 gRNAs. Key features This method provides a new technique for screening ncRNAs and establishing chromatin interaction networks. The target gene for this method can be any gene of interest and any site in the entire genome. This method can be further extended to detect RNAs, DNAs, and proteins that interact with target genes. Graphical overview.