Similar Substrates Research Articles

Deformation and cracking phenomena in cold sprayed 6061 Al alloy powders with nanoscale aluminum oxide films

This paper presents deformation and cracking phenomena associated with the cold spray of nanoscale surface oxide layers associated with the cold spray of 6061 Al alloy powders. The structure of the top surface oxide film was revealed via Focused Ion Beam (FIB), Transmission Electron Microscopy (TEM), and X-ray Photoelectron Spectroscopy (XPS). The observations show the oxide layer characteristics most likely matches crystalline γ-Al2O3. Phenomena associated with surface contacts, contact-induced elastic-plastic deformation, heating, and cracking are then studied using a combination of analytical models, Finite Element Analysis (FEA) and Molecular Dynamics (MD) simulations. MD simulations are also used to obtain estimates of oxide film young's moduli, strains to failure, and the fracture energies of oxide films. The results from the MD simulations and microscopy observations are incorporated into a bi-linear Johnson- Cook finite element simulations of cold spray contact-induced deformation and cracking due to the impact of aluminum powder particles with nanoscale oxide layers and similar substrates with nanoscale oxide layers. The powder impacts are shown to result in localized splat deformation and heating, along with the cracking of the oxide layers that expose fresh metallic surfaces to high temperature surface contacts (at temperatures above the recrystallization temperature for aluminum/6061 Al) that can give rise to bonding and mechanical interlocking. The implications of the results are then discussed for the design of cold spray processes for the fabrication and repair of 6061 Al structures.

In-silico screening of potential target transporters for glycyrrhetinic acid (GA) via deep learning prediction of drug-target interactions

Glycyrrhetinic acid (GA) is one of the major bioactive components of the leguminous plant Glycyrrhiza spp. (Chinese licorice). The mechanism by which GA is transported within or out of cells remains completely unknown. Given the fact that living organisms harbor thousands of membrane transporters, experimental verification of membrane transporters for specific GA transporters is both laborious and time consuming. In this study, we carried out virtual screening of membrane transporters to predict their probability of binding with GA. Our mining strategy relied on deep learning prediction of drug-target interactions (DTI) combined with molecular docking. We used combinations of drug and protein descriptors to create 12 deep learning models to compare the performance of each descriptor followed by the evaluation of predictions via molecular docking scores. The ABC transporter BPT1p was predicted as a target that achieved the highest docking score among all tested proteins (−10.9 kcal/mol). Additionally, the models predictions of ABC transporters such as NPC1, MRP1, MRP3, NPC1L1, ABCA3 and PDR11, which have similar substrates reflect the ability of the models to learn the sequence features associated with similar compound molecules. We conclude that, our mining method was an effective approach for predicting membrane transporter substrates.

Selective recognition of methyl viologen by an endo-functionalized naphthobox

Highly selective binding of structurally similar substrates is common for biomolecular recognition, but is often challenging to realize in synthetic hosts. Herein, we report highly selective binding of methyl viologen over other analogues by an endo-functionalized naphthobox. X-ray single crystal structure and Density Functional Theory (DFT) calculations revealed that the endo-functionalized groups in the cavity of the naphthobox is important for the high binding selectivity through the formation of multiple CH⋅⋅⋅N, CH⋅⋅⋅π, and π⋅⋅⋅π interactions with methyl viologen.

Arthropod Communities on Young Vegetated Roofs Are More Similar to Each Other Than to Communities at Ground Level

Vegetated roofs are human-manufactured ecosystems and potentially promising conservation tools for various taxa and habitats. Focussing on arthropods, we conducted a 3 year study on newly constructed vegetated roofs with shallow substrates (up to 10 cm) and vegetation established with pre-grown mats, plug plants and seeds to describe pioneer arthropod communities on roofs and to compare them with ground level communities. We vacuum sampled arthropods from the roofs and nearby ground level sites with low, open vegetation, i.e., potential source habitats. We showed that the roofs and ground sites resembled each other for ordinal species richness but differed in community composition: with time the roofs started to resemble each other rather than their closest ground level habitats. Species richness increased with time on roofs and at ground level, but the roofs had consistently less species than the ground sites and only a few species were unique to the roofs. Also, the proportion of predators increased on roofs, while not at ground level. We conclude that vegetated roofs established with similar substrates and vegetation, filter arthropods in a way that produces novel communities that are different from those at ground level but similar to one another. The role of these insular communities in species networks and ecosystem function remains to be investigated.

Electrochemical α-C(sp3)-H/O-H cross-coupling of isochromans and alcohols assisted by benzoic acid.

Isochroman moieties are present in a wide variety of biologically active molecules, but converting isochromans to α-substituted derivatives under mild conditions is challenging. Herein, we report a mild, convenient protocol for synthesis of α-alkoxy isochroman derivatives by means of electrochemical α-C(sp3)-H/O-H cross-coupling reactions of isochromans and alcohols in the presence of benzoic acid, which facilitated the electro-oxidation process and increased the product yield. Various alcohols and isochromans, as well as other structurally similar substrates, gave moderate to high yields of the desired coupling products, and the reaction could be carried out on a gram scale.

The involvement of monocular channels in the face pareidolia effect.

Studies examining the neural mechanisms of face perception in humans have mainly focused on cortical networks of face-selective regions. However, subcortical regions are known to play a significant role in face perception as well. For instance, upon presenting pairs of faces sequentially to the same eye or to different eyes, superior performance is observed in the former condition. This superiority was explained by monocular, pre-striate processing of face stimuli. One of the intriguing face-related effects is the face pareidolia phenomenon, wherein observers perceive faces in inanimate objects. In this study, we examined whether face pareidolia involves similar low-level neural substrates to those that are involved in face perception. We presented participants with pairs of houses or face-like houses using a stereoscope to manipulate the information presented to each eye and asked them to determine whether the stimuli were similar or different. We managed to examine the contribution of monocular channels (mostly subcortical) in processing face-like stimuli. We hypothesized that besides their involvement in actual face perception, subcortical structures are engaged in face pareidolia as well. To test our hypothesis, we conducted three experiments to replicate and strengthen the reliability of our results and rule out alternative explanations. We demonstrated a perceptual benefit when presenting similar face-like houses to the same eye in comparison to their presentation to different eyes. This finding matches previous results found for images of real faces and indicates subcortical involvement not only in face perception but also in processing face-like objects.

Hysteretic Magnetoresistance in a Non-Magnetic SrSnO3 Film via Thermal Coupling to Dynamic Substrate Behavior.

Hysteretic magnetoresistance (MR) is often used as a signature of ferromagnetism in conducting oxide films and heterostructures. Here, magnetotransport is investigated in a nonmagnetic La-doped SrSnO3 film. A 12 nm La:SrSnO3/2 nm SrSnO3/GdScO3 (110) film with insulating behavior exhibited a robust hysteresis loop in the MR at T < 5 K accompanied by an anomaly at ∼±3 T at T < 2.5 K. Furthermore, MR with the field in-plane yielded a value exceeding 100% at 1.8 K. Using detailed temperature-, angle- and magnetic field-dependent resistance measurements, we illustrate the origin of hysteresis is not due to magnetism in the film but rather is associated with the magnetocaloric effect of the substrate. Given GdScO3 and similar substrates are commonly used, this work highlights the importance of thermal coupling to processes in the substrates which must be carefully accounted for in the data interpretation for heterostructures utilizing these substrates.

Surface-enhanced Raman scattering with gold-coated silicon nanopillars arrays: The influence of size and spatial order

Nanopillars have been extensively explored as promising substrates for surface-enhanced Raman scattering (SERS) owing to their high sensitivity and excellent reproducibility. Most of the researches have been focused on the fabrication methods of nanopillars, and the dependences of SERS effects on geometrical size and spatial order are rarely investigated. In this work, SERS properties of nanopillars with different sizes (115–185 nm) and spatial orders (square and rhombus orders) have been studied. The work has shown that the nanopillars not only have high enhancement capability and high signal reproducibility, but also the enhancement is insensitive to the size and spatial orders. The measured enhancement factors (EFs) are 2.3–4.0 × 106 and signal reproducibility (relative standard deviation, RSD) are ∼ 5.2%–6.9%, which are among the best of the similar SERS substrates reported. The variation of SERS intensity was as low as approximately 4.8% with the variation of pillar size from 115, 135, 145, to 160 nm. The insensitiveness and high reproducibility have been ascribed to the combined excitation of localized surface plasmon resonance (LSPR) and propagating surface plasmons (SPPs) of the nanopillars. Optical properties of the nanopillars are studied both experimentally and numerically to understand the physics behind the SERS performance.

Modulation of offset analgesia in patients with chronic pain and healthy subjects- a systematic review and meta-analysis.

Offset analgesia (OA) induces a brief pain inhibition and studies suggest OA impairment in patients with chronic pain when compared to healthy subjects. Conditioned pain modulation remains the most studied descending pain inhibitory control mechanism and is modulated by centrally-acting analgesics. Since OA may be mediated by similar neural substrates as conditioned pain modulation, understanding if OA is a peripheral or central proxy of pain modulation is important. The modulatory effect of centrally-acting drugs on OA in healthy and chronic pain populations has not yet been systematically reviewed and meta-analyzed, and this systematic review and meta-analysis aimed to identify studies employing interventions for modulating OA magnitude. A systematic search of PubMed, Embase, Web of Science, and the Cochrane Library yielded 146 records of which 11 (172 healthy pain-free subjects, 106 chronic pain patients) were eligible for qualitative synthesis, and 10 for meta-analysis on overall modulatory effect of interventions on OA, and subgroup analysis of patients and healthy pain-free subjects. Risk of bias was evident for study participation and study confounding in the included studies. Several different methods for assessing and calculating OA magnitude were identified, which may affect interpretability of findings and warrants standardization. The meta-analysis showed no modulatory effects on OA overall (standardized mean difference (SMD) [95%CI]: 0.04 [-0.22, 0.30], Z=0.29, p=0.77), or in the subgroup analysis for patients (SMD [95%CI]:-0.04 [-0.63, 0.71], Z=0.13, p=0.90) or healthy pain-free subjects (SMD [95%CI]: 0.01 [-0.21, 0.24], Z=0.11, p=0.91). Moderate to substantial heterogeneity was found for the overall analysis (I2=47%, p=0.03) and patient subgroup analysis (I2=75%, p=0.003). The current systematic review and meta-analysis conclude that centrally-acting drugs and exercise do not influence OA. Evidence on the peripheral contribution to OA response requires further investigations. Preclinical models of OA should be established to identify the neurophysiology and -biology behind OA.

System dynamics kinetic model for predicting biogas production in anaerobic condition: Preliminary assessment.

This preliminary assessment of a grey-box model, was predicated on system dynamics principles and developed using Vensim® DSS software. The purpose is to predict biogas production under anaerobic conditions for energy utilization at the design stage. To describe the process of a developed system dynamics model to predict biogas production under anaerobic conditions. This method involves two-stage kinetics of the biogas production process in anaerobic conditions using the first-order and Gompertz functions. The model is depicted in two parts: causal loop diagram and stock-flow diagram. The causal loop diagram describes the anaerobic digestion process a substrate undergoes for the production of biogas, while stock-flow diagram depicts basic building blocks of the dynamic behavior of an anaerobic digestion process. Primary data is from a laboratory-scale experiment of biogas production using vegetal wastes, while the secondary one is from the literature on studies using similar substrates. Primary and secondary data are used to validate and stimulate the developed model. The kinetic model shows the substrate being reduced exponentially with increasing time; consumption of substrate and production of methane and carbon dioxide follows exponential growth and decay pattern, with carbon dioxide production starting early compared to methane, and was produced at a rate faster due to the strong and resilient characteristics of fermentative microorganisms. Comparing data from empirical and model simulation shows some close relationship, though not too perfectly. Both results reflect signs of inhibitions occurring within the substrates in the digester under anaerobic conditions explaining the low methane yield or instability.

Structure and Functional Differences of Cysteine and 3-Mercaptopropionate Dioxygenases: A Computational Study.

Thiol dioxygenases are important enzymes for human health; they are involved in the detoxification and catabolism of toxic thiol-containing natural products such as cysteine. As such, these enzymes have relevance to the development of Alzheimer's and Parkinson's diseases in the brain. Recent crystal structure coordinates of cysteine and 3-mercaptopropionate dioxygenase (CDO and MDO) showed major differences in the second-coordination spheres of the two enzymes. To understand the difference in activity between these two analogous enzymes, we created large, active-site cluster models. We show that CDO and MDO have different iron(III)-superoxo-bound structures due to differences in ligand coordination. Furthermore, our studies show that the differences in the second-coordination sphere and particularly the position of a positively charged Arg residue results in changes in substrate positioning, mobility and enzymatic turnover. Furthermore, the substrate scope of MDO is explored with cysteinate and 2-mercaptosuccinic acid and their reactivity is predicted.

The continuity of effect of schizophrenia polygenic risk score and patterns of cannabis use on transdiagnostic symptom dimensions at first-episode psychosis: findings from the EU-GEI study

Diagnostic categories do not completely reflect the heterogeneous expression of psychosis. Using data from the EU-GEI study, we evaluated the impact of schizophrenia polygenic risk score (SZ-PRS) and patterns of cannabis use on the transdiagnostic expression of psychosis. We analysed first-episode psychosis patients (FEP) and controls, generating transdiagnostic dimensions of psychotic symptoms and experiences using item response bi-factor modelling. Linear regression was used to test the associations between these dimensions and SZ-PRS, as well as the combined effect of SZ-PRS and cannabis use on the dimensions of positive psychotic symptoms and experiences. We found associations between SZ-PRS and (1) both negative (B = 0.18; 95%CI 0.03–0.33) and positive (B = 0.19; 95%CI 0.03–0.35) symptom dimensions in 617 FEP patients, regardless of their categorical diagnosis; and (2) all the psychotic experience dimensions in 979 controls. We did not observe associations between SZ-PRS and the general and affective dimensions in FEP. Daily and current cannabis use were associated with the positive dimensions in FEP (B = 0.31; 95%CI 0.11–0.52) and in controls (B = 0.26; 95%CI 0.06–0.46), over and above SZ-PRS. We provide evidence that genetic liability to schizophrenia and cannabis use map onto transdiagnostic symptom dimensions, supporting the validity and utility of the dimensional representation of psychosis. In our sample, genetic liability to schizophrenia correlated with more severe psychosis presentation, and cannabis use conferred risk to positive symptomatology beyond the genetic risk. Our findings support the hypothesis that psychotic experiences in the general population have similar genetic substrates as clinical disorders.

Long-term fertilization modifies the mineralization of soil organic matter in response to added substrate

The turnover of SOC in soils is strongly influenced by the availability of substrate and nutrients, especially nitrogen (N) and phosphorus (P). Here, we assessed how long-term fertilization modified SOM mineralization in response to added substrate in a tropical forest. We carried out a 90-day incubation study in which we added two structurally similar compounds which differed in microbial metabolic availability: corn cellulose or corn starch to soils collected from a long-term (11 years) factorial N and P fertilization experiment site in a tropical forest in south China. We measured total soil mineralization rate (CO2 efflux) to characterize SOM mineralization and using 13C isotope signatures to determine the source of the CO2 (original soil C or added substrate) and assessed changes in extracellular enzyme activities: acid phosphomonoesterase (AP), β-1,4-glucosidase (BG), β-1,4- N-acetaminophen glucosidase (NAG), phenol oxidase (PHO) and peroxidase (PER), and microbial biomarkers to determine whether nutrient stoichiometry and decomposer communities explain differences in SOM mineralization rates. Total C mineralization increased substantially with substrate addition, particularly cellulose (5.38, 7.13, 5.58 and 5.37 times for N, P, NP fertilization and CK, respectively) compared to no substrate addition, and original soil C mineralization was further enhanced in long-term N (3.40% and 5.18% for cellulose and starch addition, respectively) or NP (35.11% for cellulose addition) fertilized soils compared to control treatment. Enzyme activities were stimulated by the addition of both substrates but suppressed by P-fertilization. Addition of both substrates increased microbial investment in P-acquisition, but only starch addition promoted C investment in N-acquisition. Finally, fungal abundance increased with substrate addition to a greater extent than bacterial abundance, particularly in cellulose-amended soils, and the effect was amplified by long-term fertilization. Our findings indicate that SOM mineralization might be enhanced in N and P enrichment ecosystems, since the litter input can liberate microbes from C limitation and stimulate SOM mineralization if N and P are sufficient. Our study further demonstrates that structurally similar substrates can have distinct effects on SOM mineralization and the extent of SOM mineralization is strongly dependent on elemental stoichiometry, as well as the resource requirements of microbial decomposers.

Rational Multitargeted Drug Design Strategy from the Perspective of a Medicinal Chemist.

The development of multitarget-directed ligands (MTDLs) has become a widely focused research topic, but rational design remains as an enormous challenge. This paper reviews and discusses the design strategy of incorporating the second activity into an existing single-active ligand. If the binding sites of both targets share similar endogenous substrates, MTDLs can be designed by merging two lead compounds with similar functional groups. If the binding sites are large or adjacent to the solution, two key pharmacophores can be fused directly. If the binding regions are small and deep inside the proteins, the linked-pharmacophore strategy might be the only way. The added pharmacophores of second targets should not affect the binding mode of the original ones. Moreover, the inhibitory activities of the two targets need to be adjusted to achieve an optimal ratio.

Testing standard growth substrates for establishing native dry sandy grassland species on extensive green roofs in Northern Germany

Green roofs are known to mitigate the negative effects of urban consolidation by offering diverse ecosystem functions compared to non-vegetated roofs. However, the support for native biodiversity might be improved by using native plant species. In a mesocosm experiment, we studied the suitability of three commercial green-roof growth substrates for the establishment of 27 native plant species from dry sandy grasslands of northwestern Germany over the course of four years. The substrates were mineral-based, but differed in the layering of organic matter. Total establishment rates reached 44–59% in Year 4, indicating the general suitability of the substrates. During the first weeks after seeding, with light irrigation, the vascular plant cover was greater in the similar substrates Zincolit® Plus (Z) and Zincolit® Plus-Leicht (ZL) with their compost-based organic mulch layers than in the substrate Sedumteppich (ST) with its organic matter evenly admixed with the mineral aggregates. In Years 2 and 3, however, the vascular plant cover was greater in the ST substrate, likely due to the better availability of water and nutrients from the organic matter compared to the dry surface-mulch layer variants Z and ZL. After severe drought events, the decline in plant cover was more pronounced in the ST substrate, likely representing a trade-off between lush growth and a susceptibility to drought. An indicator-species analysis revealed differences in species composition between the ST and Z/ZL substrates. Annual plant species were indicators of the ST substrate. Perennials, such as Thymus pulegioides and Achillea millefolium, were typical of the Z and ZL substrates. In addition to the general suitability of the tested standard substrates for target species establishment, the study indicated that a combination of different layers of substrate components resulted in different vegetation patterns that may have a positive effect on green-roof biodiversity.

Comparison of the modulatory effects of three structurally similar potential prebiotic substrates on an in vitro multi-species oral biofilm

Previous research identified potential prebiotic substrates for oral health like the structural analogues N-acetyl-d-mannosamine (NADM) and N-acetyl-d-glucosamine (NADG). The main hypothesis of the current study was twofold. Firstly, it was hypothesized that the modulatory effects of NADM are not limited to changes in multi-species oral biofilm composition, but also include effects on metabolism, virulence, and inflammatory potential. Secondly, the presence and orientation of their N-acetyl group could play a role. Therefore, a comparison was made between the effects of NADM, NADG and d-(+)-mannose on multi-species oral biofilms. Besides a beneficial compositional shift, NADM-treated biofilms also showed an altered metabolism, a reduced virulence and a decreased inflammatory potential. At a substrate concentration of 1 M, these effects were pronounced for all biofilm aspects, whereas at ~ 0.05 M (1%(w/v)) only the effects on virulence were pronounced. When comparing between substrates, both the presence and orientation of the N-acetyl group played a role. However, this was generally only at 1 M and dependent on the biofilm aspect. Overall, NADM was found to have different effects at two concentrations that beneficially modulate in vitro multi-species oral biofilm composition, metabolism, virulence and inflammatory potential. The presence and orientation of the N-acetyl group influenced these effects.

Distribution of benthic foraminifera in an oceanic (Darwinian) barrier reef lagoon, Bora Bora, French Polynesia

Bora Bora, French Polynesia, represents the classic oceanic (Darwinian) barrier reef system, complete with a deep and extensive lagoon. The foraminiferal faunas of such oceanic barrier reef lagoons have never been studied in detail. How they relate to atoll lagoons and more broadly to coral reef lagoons in general is not known. Back-reef lagoon samples from Bora Bora were studied for their foraminiferal content. The fauna contains sparse larger symbiont bearing foraminifera, and most samples are dominated by a textulariid fauna of mainly Textularia gr. sp. 1, Textularia gr. foliacea and Textularia oceanica. Statistical analyses revealed three main assemblages, a Textularia-dominated deep central lagoon assemblage, an outer shallow lagoon assemblage characterised by abundant porcelaneous taxa, and a narrow embayment assemblage with abundant Elphidium. Although the species at Bora Bora are common to the Pacific Ocean, the Textularia-dominated assemblages are markedly different from the lagoon assemblages reported from atoll and non-oceanic barrier reef lagoons in the Pacific. These unique assemblages are hypothesised to have evolved due to interactions of the central volcanic island with the lagoon system, likely via the run-off of fine sediment and nutrients. Thereby, the prerequisites for abundant larger, symbiont-bearing foraminifera, which prefer clear, nutrient-poor waters, are largely unavailable. Despite similar substrates and facies, differences between species compositions indicate subtly different disconnected western and eastern deep lagoon environments, possibly due to differences in connectivity to the open ocean. This study provides the first detailed account of the foraminifera from the lagoon of an oceanic barrier reef lagoon.

A stable and plug-and-play aluminium/titanium dioxide/metal-organic framework/silver composite sheet for sensitive Raman detection and photocatalytic removal of 4-aminothiophenol

The sensitive detection and rapid removal of 4-aminothiophenol (4-ATP, a poisonous pesticide) demand special design to potential substrates. Herein, a metal-organic framework (ZIF-8) and Ag nanoparticles were fabricated one by one on the TiO2 coated Al sheet, and thus the Al–TiO2-ZIF-8-Ag sheet with sandwich structure was successfully synthesized. The cost-effective Al–TiO2-ZIF-8-Ag sheet (3.7 wt% Ag) possessed a low detection concentration of 1 × 10−9 M towards 4-ATP, and surface-enhanced Raman scattering (SERS) analytical enhanced factor (AEF) of the Al–TiO2-ZIF-8-Ag was 2.6 × 106, which was higher than other similar substrates. Furthermore, 4-ATP can be selectively and repeatedly detected on the Al–TiO2-ZIF-8-Ag even through it was in real samples. It indicated that the Al–TiO2-ZIF-8-Ag was a very active and stable SERS materials for the monitoring of 4-ATP. Importantly, the substrate exhibited faster and more efficient photocatalytic activity for 4-ATP degradation. The SERS and photocatalytic mechanisms of 4-ATP on the Al–TiO2-ZIF-8-Ag substrate were proposed. The cost-effective Al–TiO2-ZIF-8-Ag sheet with double function is plug-and-play, and could be used in the detection and treatment of pollutants in wastewater.

Microstructure scaling in metal-insulator-transitions of atomic layer deposited VO2 films

Thin films of VO2 are grown by atomic layer deposition onto low-cost amorphous substrates and compared to sputtered and pulsed laser deposited films on similar substrates. They were each analyzed in terms of the scaling with grain size of the resistivity change across the metal insulator transition, its thermal hysteresis and its full width at half maximum of the slopes. This finds a similar dependence and suggests that the cause is the sintering processes of these films during their deposition and annealing, which determine their microstructural properties.

Semi-enclosed microfluidic device on glass-fiber membrane with enhanced signal quality for colorimetric analyte detection in whole blood

Microfluidic analytical devices manufactured on paper and similar inexpensive substrates (µ-PADs) have shown considerable promise for disease diagnostics in resource-limited regions. However, current commercialization approaches can be improved substantially by addressing existing technical challenges associated with µ-PADs. Among these, off-device plasma separation from whole blood is a critical challenge in µ-PAD technology that limits commercialization. Existing µ-PADs made by combining multiple components require extra fabrication steps and manufacturing material. Our approach utilizes a two-step plasma process to fabricate single-layer semi-enclosed µ-PADs directly on a commercially available blood plasma separation membrane to incorporate blood plasma separation functionality into the device. The semi-enclosed µ-PADs are bonded with low-cost adhesive plastic tape to provide mechanical support to the device and make it more mechanically robust for field applications. Detection zones of the µ-PADs have also been modified with a cellulose nanocrystal (CNC) to increase colorimetric signal homogeneity, thus enhancing signal quality. The CNC-modified µ-PADs have been used for colorimetric detection of two model analytes (glucose and albumin) in whole blood. Colorimetric signals for both glucose and albumin from whole blood samples were consistent with the calibration curves generated using stock solutions.