Similar Pattern Of Change Research Articles

A Small Non-Coding RNA Mediates Transcript Stability and Expression of Cytochrome bd Ubiquinol Oxidase Subunit I in Rickettsia conorii.

Small regulatory RNAs (sRNAs) are now widely recognized for their role in the post-transcriptional regulation of bacterial virulence and growth. We have previously demonstrated the biogenesis and differential expression of several sRNAs in Rickettsia conorii during interactions with the human host and arthropod vector, as well as the in vitro binding of Rickettsia conorii sRNA Rc_sR42 to bicistronic cytochrome bd ubiquinol oxidase subunits I and II (cydAB) mRNA. However, the mechanism of regulation and the effect of sRNA binding on the stability of the cydAB bicistronic transcript and the expression of the cydA and cydB genes are still unknown. In this study, we determined the expression dynamics of Rc_sR42 and its cognate target genes, cydA and cydB, in mouse lung and brain tissues during R. conorii infection in vivo and employed fluorescent and reporter assays to decode the role of sRNA in regulating cognate gene transcripts. Quantitative RT-PCR revealed significant changes in the expression of sRNA and its cognate target gene transcripts during R. conorii infection in vivo, and a greater abundance of these transcripts was observed in the lungs compared to brain tissue. Interestingly, while Rc_sR42 and cydA exhibited similar patterns of change in their expression, indicating the influence of sRNA on the mRNA target, the expression of cydB was independent of sRNA expression. Further, we constructed reporter plasmids of sRNA and cydAB bicistronic mRNA to decipher the role of sRNA on CydA and CydB expression. We observed increased expression of CydA in the presence of sRNA but detected no change in CydB expression in the presence or absence of sRNA. In sum, our results demonstrate that the binding of Rc_sR42 is required for the regulation of cydA but not cydB. Further studies on understanding the influence of this interaction on the mammalian host and tick vector during R. conorii infection are in progress.

Sex and diet-dependent gene alterations in human and rat brains with a history of nicotine exposure.

Chronic nicotine exposure induces changes in the expression of key regulatory genes associated with metabolic function and neuronal alterations in the brain. Many bioregulatory genes have been associated with exposure to nicotine, but the modulating effects of sex and diet on gene expression in nicotine-exposed brains have been largely unexplored. Both humans and rodents display motivation for nicotine use and the emergence of withdrawal symptoms during abstinence. Research comparing pre-clinical models with human subjects provides an important opportunity to understand common biomarkers of the harmful effects of nicotine as well as information that may help guide the development of more effective interventions for nicotine cessation. Human postmortem dorsolateral prefrontal cortex (dLPFC) tissue BA9 was collected from female and male subjects, smokers and non-smokers (N = 12 per group). Rat frontal lobes were collected from female and male rats that received a regular diet (RD) or a high-fat diet (HFD) (N = 12 per group) for 14 days following implantation of a osmotic mini-pump (Alzet) that delivered nicotine continuously. Controls (control-s) received a sham surgical procedure. RNA was extracted from tissue from human and rat samples and reversed-transcribed to cDNA. Gene expression of CHRNA10 (Cholinergic receptor nicotinic alpha 10), CERKL (Ceramide Kinase-Like), SMYD1 (SET and MYD Domin Containing 1), and FA2H (Fatty Acid 2-Hydrolase) in humans was compared to rats in each subset of groups and quantified by qPCR methods. Additionally, protein expression of FA2H was analyzed by immunohistochemistry (IHC) in human dLPFC. Humans with a history of smoking displayed decreased CHRNA10 (p = 0.0005), CERKL (p ≤ 0.0001), and SMYD1 (p = 0.0005) expression and increased FA2H (p = 0.0097) expression compared to non-smokers (p < 0.05). Similar patterns of results were observed in nicotine exposed vs. control rats. Interestingly, sex-related differences in gene expression for CERKL and FA2H were observed. In addition, ANCOVA analysis showed a significant effect of nicotine in a sex-different manner, including an increase in CERKL in male and female rats with RD or HFD. In rats exposed to an HFD, FA2H gene expression was lower in nicotine-treated rats compared to RD rats treated with nicotine. Protein expression of FA2H (p = 0.001) by IHC was significantly higher in smokers compared to non-smokers. These results suggest that a history of long-term nicotine exposure in humans alters the expression of sphingolipid metabolism-related (CERKL, SMYD1, and FA2H) and neuronal (CHRNA10) marker genes similarly as compared to rats. Sex- and diet-dependent differences appear in nicotine-exposed rats, critical in regulating sphingolipid metabolism and nicotinic acetylcholine receptors. This research enhances the construct validity of rat models of nicotine usage by showing a similar pattern of changes in gene expression in human subjects with a smoking history.

Neuroanatomical and neurocognitive changes associated with subjective cognitive decline.

Subjective Cognitive Decline (SCD) can progress to mild cognitive impairment (MCI) and Alzheimer's disease (AD) dementia and thus may represent a preclinical stage of the AD continuum. However, evidence about structural changes observed in the brain during SCD remains inconsistent. This cross-sectional study aimed to evaluate, in subjects recruited from the CompAS project, neurocognitive and neurostructural differences between a group of forty-nine control subjects and forty-nine individuals who met the diagnostic criteria for SCD and exhibited high levels of subjective cognitive complaints (SCCs). Structural magnetic resonance imaging was used to compare neuroanatomical differences in brain volume and cortical thickness between both groups. Relative to the control group, the SCD group displayed structural changes involving frontal, parietal, and medial temporal lobe regions of critical importance in AD etiology and functionally related to several cognitive domains, including executive control, attention, memory, and language. Despite the absence of clinical deficits, SCD may constitute a preclinical entity with a similar (although subtle) pattern of neuroanatomical changes to that observed in individuals with amnestic MCI or AD dementia.

Children with handwriting difficulties: Impact of cognitive strategy training for acquisition of accurate alphabet-letter-writing

Introduction: An occupational therapy handwriting programme integrating cognitive strategy training with alphabet-letter-writing instruction was found more effective than in-class instruction alone for early writers. This study investigated the impact of cognitive strategy training for children struggling to correctly write alphabet-letters. Method: Participants were ten children in the first 2 years of formal schooling identified with challenges in alphabet-letter-writing. A nonconcurrent A1BA2 single system research design was used. The A Phases represented within-class handwriting instruction only and B Phase represented additional handwriting intervention which included cognitive strategy training. The Perceive, Recall, Plan and Perform (PRPP) System of Task Analysis (Stage 2) was the repeated outcome measure. Results: Results revealed all ten children demonstrated a similar pattern of change in cognitive strategy application which was concomitant with improvement and mastery in writing all 26-lowercase alphabet-letters from memory. Cognitive strategies which exhibited the greatest impact on acquiring and executing accurate alphabet-letter-writing skills were those related to planning, memory, attention and sensory perception. The least influential were those associated with generating and controlling motor actions. Conclusion: This study provides beginning support for including cognitive strategies training concurrently with alphabet-letter-writing instruction to assist children struggling to write alphabet-letters.

The relationship of land tenure, land use and land cover changes in Lake Victoria basin

Water basins characterise both physical and social environmental aspects such as land tenure. As such, the basins extend beyond spatial units of physical resources and human relations analysis to policy research and reform units. The comprehensive view of water basins in research goes along with an observed increase in anthropogenic-driven changes, such as land use and land cover changes, and cases of ineffective remedial measures to the adverse change, such as through applying integrated watershed management approaches. The human-induced land cover changes affect the water basin’s biodiversity, for instance, contributing to an increase in zoonotic disease outbreaks like coronaviruses. The Lake Victoria basin exhibits similar patterns of change and effects due to, among other factors, land tenure, whose contribution is less known empirically. Therefore, this paper integrates satellite imagery and catchment survey data to examine the relationship between land tenure and land uses and land cover changes in the Lake Victoria basin of Eastern Africa.Additionally, explore the contextual character and role of three land tenure systems of Customary, Native freehold and Mailo found in the Uganda country segment of the basin in explaining the outcomes. The aim is to provide information that, among other benefits, improves water basin management and governance. The results indicate a statistically significant relationship exists between the perceived extent of land use and land cover change; drivers of change; the extent of adopting sustainable land-use practices, and the prevailing land tenure. Though with different tenure systems, the three case study water catchments experienced adverse land use and cover changes. The changes mainly affected land tenure indicative land use and cover classes, prominently on the Customary, Mailo, and Native freehold land tenure systems. However, marginal differences occur among the land tenure systems, as the systems feature both de jure and de facto systems and an orientation towards customary tenure characters. The situation likely explains the observed closeness in perceptions regarding the role and relationship between land tenure and land use and cover changes, tenure systems character, perceived drivers of change and eventual outcomes, including the sustainable land use practices adoption. In addition to explaining the land use and cover change, land tenure is an essential tool for restoration and sustainable basin development and sustainability. We, thus, recommend land tenure responsiveness in water basin management approaches for sustainable societal development.

Simulations of the Holocene climate in Europe using an interactive downscaling within the iLOVECLIM model (version 1.1)

Abstract. This study presents the application of an interactive downscaling in Europe using iLOVECLIM (a model of intermediate complexity), increasing its atmospheric resolution from 5.56 to 0.25∘ kilometric. A transient simulation using the appropriate climate forcings for the entire Holocene (11.5–0 ka BP) was done for both the standard version of the model and with an interactive downscaling applied. Our results show that simulations from downscaling present spatial variability that agrees better with proxy-based reconstructions and other climate models as compared to the standard model. The downscaling scheme simulates much higher (by at least a factor of 2) precipitation maxima and provides detailed information in mountainous regions. We focus on examples from the Scandes mountains, the Alps, the Scottish Highlands, and the Mediterranean. The higher spatial resolution of the downscaling provides a more realistic overview of the topography and gives local climate information, such as precipitation and temperature gradient, that is important for paleoclimate studies. With downscaling, we simulate similar trends and spatial patterns of the precipitation changes reconstructed by other proxy studies (for example in the Alps) as compared to the standard version. Our downscaling tool is numerically cheap, implying that our model can perform kilometric, multi-millennial simulations and is suitable for future studies.

Trajectories of quality of life in breast cancer survivors during the first year after treatment: a longitudinal study

BackgroundAlthough quality of life (QOL) improves over time for most breast cancer patients after their treatment, some patients may show different patterns of QOL. Beyond determining distinct QOL trajectories, identifying characteristics of patients who have different trajectories can help identify breast cancer patients who may benefit from intervention. We aimed to identify trajectories of QOL in breast cancer patients for one year after the end of primary treatment, to determine the factors influencing these changes.MethodsThis longitudinal study recruited 140 breast cancer patients. Patients' QOL, symptom experience, self-efficacy, and social support were assessed using the Functional Assessment of Cancer Therapy Scale-G, Memorial Symptom Assessment Scale-Short Form, Self-Efficacy Scale for Self-Management of Breast Cancer, and Interpersonal Support Evaluation List-12. Data were collected immediately after the end of primary treatment (T1) and at three (T2), six (T3), and 12 months (T4) after primary treatment. Group-based trajectory modeling was used to identify distinct subgroups of patients with similar patterns of QOL change after treatment. A one-way analysis of variance was used to determine which variables were associated with trajectory membership. A multinomial logistic regression was performed to identify factors associated with trajectory group membership.ResultsWe analyzed 124 patients (mean age: 48.75 years). Latent class analysis of the QOL identified three trajectory groups: the low QOL group (n = 27; 21.1%), moderate QOL group (n = 57; 45.3%), and high QOL group (n = 40; 33.6%). The low QOL group showed consistently low QOL after the end of primary treatment, and the moderate QOL group showed a slight decrease in QOL from T1 to T3, which returned to the T1 level at T4. The high QOL group maintained a consistently high QOL. By multinomial logistic regression, psychological symptoms (odds ratio [OR] 0.46, 95% confidence interval [CI] 0.22–0.99) predicted a moderate QOL, and both psychological symptoms (OR 0.19, 95% CI 0.07–0.51) and belonging support (OR 1.60, 95% CI 1.06–2.39) predicted a high QOL.ConclusionIdentifying high-risk groups for reduced QOL after the end of primary treatment is necessary. Moreover, psychosocial interventions should be provided to alleviate psychological symptoms and increase belonging support to enhance patients' QOL.Trial registration Not registered.

Training and detraining management of performance measures by Yoga among middle-aged blue-collar men

To observe the effect of yogasanas and yoga breathing maneuvers (YBM) on body flexibility, balance, muscular strength and reaction time on middle aged blue-collar individuals, 8 healthy blue collar, males (age 37±6.2 years) were trained in yoga for 12 weeks. First 6 weeks, they practiced mostly yogasanas. From 7th week, progressively the YBM practice increased up to 12th week. Their body flexibility, balance, maximal hand grip strength (HGS) and HGS endurance at 50 % maximal strength for both hands, back -leg strength (BLS), reaction times for both visual (VRT) and auditory (ART) mode, were measured before and at the end of 6th and 12th weeks yoga training. Data was analysed by ANOVA. With more yogasanas practice, their body flexibility improved from base line 14.1±4.5 cm to 18.3±4.3 cm (P&lt;0.001, magnitude 29.8%) after 6 weeks of yoga training. With proportionately greater YBM practice, still it increased in lower magnitude (9.3%) to 20±4.6 cm (p&lt;0.001) after 12 weeks of training. Balance showed similar pattern of changes like flexibility. In VRT, baseline value of 281.4±16.4ms reduced to 271.14±16.1 ms and 251.7±15.4 ms after 6 weeks and 12 weeks respectively, indicating greater reaction time improvement by YBM. ART also showed similar pattern of changes. Left and Right HGS endurance have improved by 19% and 23.1% respectively, after 6 weeks. BLS and HGS improved in small magnitude. Detraining and age related deterioration of body flexibility, balance, reaction time and strength endurance could be controlled by yogasanas. YBM have greater positive role in reaction time.

Процессы молекулярного переноса в жидкостных питательных средах дрожжевой и пивной отраслях промышленности Часть 2. Исследование реологических свойств водных растворов мелассы и концентрированного пивного сусла

The article is a continuation of the research in the field of comprehensive study of the properties characterizing the processes of momentum, thermal energy, and mass transfer in liquid nutrient media of biotechnological production. Experimental studies of the rheological properties for liquid nutrient media have been conducted. Beet molasses with an initial concentration of dry substances (DS) of 77.7 wt.% and concentrated malt extract of beer wort with an initial concentration of DS 81 wt.% were selected as objects of research. The temperature variation range was 10 to 70℃. The viscosity of the mentioned objects was measured with a HÖPPLER® KF 3.2 ball viscometer and also for highly viscous solutions on a Rheotest RN 4.1 viscometer, as a result of which the graphical dependences on temperature and DS concentration, as well as the gradient of shear rate were obtained. It has been established that the viscosity of these two nutrient media (beet molasses and beer wort), different in the method of obtaining the composition, has not only a similar pattern of change in the dynamic viscosity coefficient depending on temperature and content of soluble solids, but also a comparable numerical range of values. The boundaries of variation for the shear rate gradient, in which the studied objects behave as non-Newtonian fluids, from 1 to 100s-1, have been determined. The results obtained are of interest both from the point of view of fundamental science about the development of the theory of the liquid state of matter and applied, in terms of engineering calculations and the design of biotechnological equipment.

Intraoperative Target-Controlled Infusion of Etomidate versus Propofol for General Anesthesia in Laparoscopic Cholecystectomy

To investigate the safety and changes of hemodynamic profiles when using etomidate for general anesthesia in laparoscopic cholecystectomy compared to propofol was the objective of the study. Adult patients who received laparoscopic cholecystectomy were retrospectively categorized as etomidate and propofol group. Time to extubation, time to eye opening, pain scores at rest, postoperative nausea, vomiting and lengths of hospital stay were studied as safety outcomes. Changes in hemodynamic profiles are evaluated by coefficients of variation of systolic blood pressure, diastolic blood pressure, mean arterial pressure and heart rates were compared between groups. 291 patients were categorized as the etomidate group and 388 patients as the propofol group. There was no significant difference in age, sex, body mass index and hemodynamic profiles at baseline between the two groups (p all>0.05). Compared to propofol, the etomidate group showed similar safety outcomes (p all>0.05). Similar change patterns in the hemodynamic profiles over time were observed between the two groups, but the coefficients of variation of systolic blood pressure, diastolic blood pressure and mean arterial pressure in the etomidate group were significantly lower than that in the propofol group (p<0.05). There was no difference in safety outcomes but a more stable hemodynamic profile when comparing intraoperative target-controlled infusion of etomidate to propofol used for general anesthesia in laparoscopic cholecystectomy.

Spatiotemporal Variation of Nitrogen Dioxide and Nighttime Light Dataset of Iranian Metropolises in the COVID-19 Outbreak

COVID-19 lockdown has caused a reduction in traffic volume and industrial activities which are the main sources of air pollution in whole of the world. As tropospheric NO2 pollutant and nighttime light (NTL) are the representative of human activities, this study focused to quantify the annual and monthly change of NO2 concentration and NTL in 14 metropolises of Iran before, during and after the lockdown months such as March, April, October and November. TROPOMI images of Sentinel-5p were used for investigation of NO2 column density in 2019, 2020 and 2021, and the variation of NTL was monitored by VIIRS images. The findings showed the majority of metropolises have an increase of NO2 concentration in March and October and a decrease in April and November in 2020 but a significant increase in 2021. The similar pattern of NTL change as NO2 was observed in the most metropolises. The correlation coefficient between NO2 concentration and NTL was calculated from 0.66 to 0.75. So, in majority of metropolises, the reduction of NO2 was observed with reduction of NTL. According to the results, reducing traffic volume as mobile source does not has an effective contribution in NO2 emission in some metropolises of Iran which the stationary sources are dominant such as Isfahan. Tehran as the capital of Iran showed the highest annual mean NO2 reduction in lockdown, this finding showed the important role of traffic volume on air quality of Tehran compared to industrial activities. The integrated application of TROPOMI and NTL data will help to better decision making for controlling and managing of air quality in country’s urban area.

Changes in Lung Volumes with Spirometric Disease Progression in COPD.

Abnormal lung volumes representing air trapping identify the subset of smokers with preserved spirometry who develop spirometric chronic obstructive pulmonary disease (COPD) and adverse outcomes. However, how lung volumes evolve in early COPD as airflow obstruction develops remains unclear. To establish how lung volumes change with the development of spirometric COPD, we examined lung volumes from the pulmonary function data (seated posture) available in the U.S. Department of Veterans Affairs electronic health records (n=71,356) and lung volumes measured by computed tomography (supine posture) available from the COPD Genetic Epidemiology (COPDGene®) study (n=7969) and the SubPopulations and InterMediate Outcome Measures In COPD Study (SPIROMICS) (n=2552) cohorts, and studied their cross-sectional distributions and longitudinal changes across the airflow obstruction spectrum. Patients with preserved ratio-impaired spirometry (PRISm) were excluded from this analysis. Lung volumes from all 3 cohorts showed similar patterns of distributions and longitudinal changes with worsening airflow obstruction. The distributions for total lung capacity (TLC), vital capacity (VC), and inspiratory capacity (IC) and their patterns of change were nonlinear and included different phases. When stratified by airflow obstruction using Global initiative for chronic Obstructive Lung Disease (GOLD) stages, patients with GOLD 1 (mild) COPD had larger lung volumes (TLC, VC, IC) compared to patients with GOLD 0 (smokers with preserved spirometry) or GOLD 2 (moderate) disease. In longitudinal follow-up of baseline GOLD 0 patients who progressed to spirometric COPD, those with an initially higher TLC and VC developed mild obstruction (GOLD 1) while those with an initially lower TLC and VC developed moderate obstruction (GOLD 2). In COPD, TLC, and VC have biphasic distributions, change in nonlinear fashions as obstruction worsens, and could differentiate those GOLD 0 patients at risk for more rapid spirometric disease progression.

Integrated aggregate turnover and soil organic carbon sequestration using rare earth oxides and 13C isotope as dual tracers

The formation, stabilization and breakdown processes of soil aggregates determine soil organic carbon (SOC) sequestration, in turn, soil aggregate dynamics are mediated by SOC changes. However, the interactions between them remain elusive. Herein, three types of 13C-labelled residues were added to two textured soils. Rare earth oxides (REOs) and 13C isotope were used as dual tracers to simultaneously track aggregate transfer pathways and SOC sequestration during a 56-day incubation period. Residue-derived CO2 followed the sequence of Vetch > Maize > Decomposed maize during the first two weeks. Residue-derived CO2 was significantly negatively correlated with the aggregate turnover time in both investigated soils (P < 0.01), indicating that aggregate turnover was a controlling factor of residue decomposition in addition to its inherent features. Generally, residue addition decreased the aggregate turnover time in the sequence of Vetch < Maize < Decomposed maize. In Red clay soil, macroaggregates attained a higher turnover rate than that of microaggregates, while a similar change pattern was not observed in Sandstone soil with residue application. Aggregates turnover occurred faster in Sandstone soil than in Red clay soil under a given residue application. The aggregate turnover time was significantly reciprocally correlated with the residue-derived C sequestration rate (P < 0.01), suggesting that aggregate turnover was the key factor in C sequestration. A C flow conceptual model was proposed, residue-derived C firstly accumulated in macroaggregates in the formation process, and then relocated from macroaggregates to microaggregates with the breakdown processes at the mid-to-late stage. This study highlights the importance of aggregate turnover in SOC sequestration and demonstrates that these interactions are further affected by residue features and soil texture.

Effects of Isosakuranetin on Pharmacokinetic Changes of Tofacitinib in Rats with N-Dimethylnitrosamine-Induced Liver Cirrhosis.

Tofacitinib, a Janus kinase 1 and 3 inhibitor, is used to treat rheumatoid arthritis. It is mainly metabolized by the cytochromes p450 (CYP) 3A1/2 and CYP2C11 in the liver. Chronic inflammation eventually leads to cirrhosis in patients with rheumatoid arthritis. Isosakuranetin (ISN), a component of Citrus aurantium L., has hepatoprotective effects in rats. This study was performed to determine the effects of ISN on the pharmacokinetics of tofacitinib in rats with N-dimethylnitrosamine-induced liver cirrhosis (LC). After intravenous administration of 10 mg/kg tofacitinib to control (CON), LC, and LC treated with ISN (LC-ISN) rats, the total area under the plasma concentration-time curves (AUC) from time zero to infinity increased by 158% in LC rats compared to those in CON rats; however, the AUC of LC-ISN rats decreased by 35.1% compared to that of LC rat. Similar patterns of AUC changes were observed in the LC and LC-ISN rats after oral administration of 20 mg/kg tofacitinib. These results can be attributed to decreased non-renal clearance (CLNR) and intestinal intrinsic clearance (CLint) in the LC rats and increased intestinal and hepatic CLint in the LC-ISN rats. Our findings imply that ISN treatment in LC rats restored the decrease in either CLNR or CLint, or both, through increased hepatic and intestinal expression of CYP3A1/2 and CYP2C11, which is regulated by the induction of pregnane X receptor (PXR) and constitutive androstane receptor (CAR).

Lexical Correspondences between Baram and Thami

Baram and Thami share seventy-two PTB reflexes. Eighteen of them are identical in both languages indicating a higher level of shared retention. Twenty-four of them are identical in Baram and Thami, depicting a higher level of shared innovations. The similarities in the remaining thirty reflexes show the shared retention, and the similar patterns of phonological changes in Baram and Thami present the situation of shared innovations. Twenty similar roots in Baram and Thami, but not Proto-Tibeto-Burman (PTB) reflexes, present further evidence of shared innovations. All the pieces of evidence justify the very close genetic affinity between Baram and Thami.

Kinematics and geomorphological changes of a destabilising rock glacier captured from close-range sensing techniques (Tsarmine rock glacier, Western Swiss Alps)

Accurately assessing landform evolution and quantifying rapid environmental changes are gaining importance in the context of monitoring techniques in alpine environments. In the European Alps, glaciers and rock glaciers are among the most characteristic cryospheric components bearing long and systematic monitoring periods. The acceleration in rock glacier velocities and the onset of destabilization processes, mainly since 1990, have raised several concerns due to the potential effects on the high alpine natural and anthropic environments. This study presents a combination of uncrewed aerial vehicle (UAV) and terrestrial laser scanning (TLS) surveys for monitoring the current changes on the quickly accelerating, destabilised Tsarmine rock glacier in the Arolla Valley, Western Swiss Alps, delivering a considerable volume of debris to a steep torrential gully. High-resolution digital elevation models (DEMs) and orthomosaics are derived from UAV image series combined with structure from motion (SfM) photogrammetry techniques. Multitemporal orthomosaics are employed for measuring spatially continuous rock glacier kinematics using image matching algorithms. Superficial displacements are evaluated with simultaneousin-situdifferential global navigation satellite system (GNSS) measurements. Elevation and volume changes are computed from TLS and UAV-derived DEMs at similar periods. Between June 2017 and September 2019, both datasets showed a similar elevation change pattern and surface thinning rates of 0.15 ± 0.04 and 0.16 ± 0.03 m yr−1, respectively. Downward of a rupture zone developing about 150 m above the front, the rock glacier doubled its overall velocity during the study period, from around 5 m yr−1between October 2016 and June 2017 to more than 10 m yr−1between June and September 2019. The kinematic information reveals striking differences in the velocity between the lower and upper rock glacier sections. The monitoring approach based on close-sensing techniques provides accurate surface velocity and volume change information, allowing an enhanced description of the current rock glacier dynamics and its surface expression.

Altered anterior insula functional connectivity in adolescent and young women with endometriosis-associated pain: Pilot resting-state fMRI study.

Endometriosis is the leading cause of chronic pelvic pain. Alterations in brain functional connectivity have been reported in adult women with endometriosis-associated pain (EAP), however, it is still unknown if similar patterns of changes exist in adolescents. In this pilot study, resting-state fMRI scans were obtained from 11 adolescent and young women with EAP and 14 healthy female controls. Using a seed-to-voxel approach, we investigated functional connectivity between the anterior insula, medial prefrontal cortex, and the rest of the brain. Furthermore, we explored whether potential functional connectivity differences were correlated with clinical characteristics including disease duration, pain intensity, and different psychosocial factors (pain catastrophizing, fear of pain, functional disability, anxiety, and depression). Our findings revealed that patients with EAP demonstrated significantly decreased connectivity between the right anterior insula and two clusters: one in the right cerebellum, and one in the left middle frontal gyrus compared to controls. Additionally, functional connectivity between the right anterior insula and the right cerebellum was positively associated with pain intensity levels. In patients with EAP, brain changes were also correlated with state anxiety and fear of pain. Our results are relevant not only for understanding the brain characteristics underlying EAP at a younger age, but also in enhancing future pain treatment efforts by supporting the involvement of the central nervous system in endometriosis.

The snoRNA-like lncRNA LNC-SNO49AB drives leukemia by activating the RNA-editing enzyme ADAR1

Long noncoding RNAs (lncRNAs) are usually 5′ capped and 3′ polyadenylated, similar to most typical mRNAs. However, recent studies revealed a type of snoRNA-related lncRNA with unique structures, leading to questions on how they are processed and how they work. Here, we identify a novel snoRNA-related lncRNA named LNC-SNO49AB containing two C/D box snoRNA sequences, SNORD49A and SNORD49B; and show that LNC-SNO49AB represents an unreported type of lncRNA with a 5′-end m7G and a 3′-end snoRNA structure. LNC-SNO49AB was found highly expressed in leukemia patient samples, and silencing LNC-SNO49AB dramatically suppressed leukemia progression in vitro and in vivo. Subcellular location indicated that the LNC-SNO49AB is mainly located in nucleolus and interacted with the nucleolar protein fibrillarin. However, we found that LNC-SNO49AB does not play a role in 2′-O-methylation regulation, a classical function of snoRNA; instead, its snoRNA structure affected the lncRNA stability. We further demonstrated that LNC-SNO49AB could directly bind to the adenosine deaminase acting on RNA 1(ADAR1) and promoted its homodimerization followed by a high RNA A-to-I editing activity. Transcriptome profiling shows that LNC-SNO49AB and ADAR1 knockdown respectively share very similar patterns of RNA modification change in downstream signaling pathways, especially in cell cycle pathways. These findings suggest a previously unknown class of snoRNA-related lncRNAs, which function via a manner in nucleolus independently on snoRNA-guide rRNA modification. This is the first report that a lncRNA regulates genome-wide RNA A-to-I editing by enhancing ADAR1 dimerization to facilitate hematopoietic malignancy, suggesting that LNC-SNO49AB may be a novel target in therapy directed to leukemia.

Associations between recreational cannabis legalization and cannabis-related emergency department visits by age, gender, and geographic status in Ontario, Canada: An interrupted time series study.

Legalization of recreational cannabis in Ontario included the legalization of flower and herbs (Phase 1, October 2018), and was followed by the deregulation of cannabis retailers and sales of edibles (Phase 2, February 2020). Research on the impact of cannabis legalization on acute care utilization is nascet; no research has investigated potential age, gender, and geographically vulnerable subgroup effects. Residents living in Northern Ontario not only have higher levels of substance use problems, but also have inadequate access to primary healthcare. Our study investigated the impact of Ontario's recreational cannabis policy (including Phase 1 and 2) on cannabis-attributable emergency department (ED) visits, and estimated the impact separately for different age and gender groups, with additional analyses focused on Northern Ontarians. We created a cohort of adults (18 and over) eligible for provincial universal health insurance with continuous coverage from 2015-2021 (n = 14,900,820). An interrupted time series was used to examine the immediate impact and month-to-month changes in cannabis-related ED visits associated with Phase 1 & 2 for each subgroup. While Northern Ontario has higher rates of cannabis-related ED visits, both Northern and Southern Ontario show similar patterns of changes. Phase 1 was associated with significant increases in adults 25-64, with the strongest increases seen in women 45-64. Month-to-month trends were flattened in most groups compared to pre-legalization. Phase 2 was associated with significant immediate increases for adults aged 18-44 in both genders, but the increases were larger in women than men. No significant month-to-month changes were detected in this period. While current preventive efforts are largely focused on reducing cannabis-related harms in youths and younger adults, our results show that adults 25-64, particularly women, have been significantly impacted by cannabis policies. Further research on gender-specific cannabis dosage and targeted interventions for adult women should be investigated. Legalization did not appear to have a differential impact on Northern versus Southern Ontario, but higher rates of ED visits in the North should be addressed.

Cardiopulmonary and cerebrovascular acclimatization in children and adults at 3800m.

Maturational differences exist in cardiopulmonary and cerebrovascular function at sea-level, but the impact of maturation on acclimatization responses to high altitude is unknown. Ten children (9.8±2.5years) and 10 adults (34.7±7.1years) were assessed at sea-level (BL), 3000m and twice over 4days at 3800m (B1, B4). Measurements included minute ventilation ( ), end-tidal partial pressures of oxygen ( ) and carbon dioxide, echocardiographic assessment of pulmonary artery systolic pressure (PASP) and stroke volume (SV) and ultrasound assessment of blood flow through the internal carotid and vertebral arteries was performed to calculate global cerebral blood flow (gCBF). At 3000m, was increased from BL by 19.6±19.1% (P=0.031) in children, but not in adults (P=0.835); SV was reduced in children (-11±13%, P=0.020) but not adults (P=0.827), which was compensated for by a larger increase in heart rate in children (+26 beatsmin-1 vs. +13 beatsmin-1 , P=0.019). Between B1 and B4, adults increased by 38.5±34.7% (P=0.006), while did not increase further in children. The rise in PASP was not different between groups; however, ∆PASP from BL was related to ∆ in adults (R2 =0.288, P=0.022), but not children. At BL, gCBF was 43% higher in children than adults (P=0.017), and this difference was maintained at high altitude, with a similar pattern and magnitude of change in gCBF between groups (P=0.845). Despite increasing in children but not adults at a lower altitude, the pulmonary vascular and cerebrovascular responses to prolonged hypoxia are similar between children and adults. KEY POINTS: Children have different ventilatory and metabolic requirements from adults, which may present differently in the pulmonary and cerebral vasculature upon ascent to high altitude. Children (ages 7-14) and adults (ages 23-44) were brought from sea level to high altitude (3000 to 3800m) and changes in ventilation, pulmonary artery systolic pressure (PASP) and cerebral blood flow (CBF) were assessed over 1 week. Significant increases in ventilation and decreases in left ventricle stroke volume were observed at a lower altitude in children than adults. PASP and CBF increased by a similar relative amount between children and adults at 3800m. These results help us better understand age-related differences in compensatory responses to prolonged hypoxia in children, despite similar changes in pulmonary artery pressure and CBF between children and adults.