Similar Parameters Research Articles

Design, synthesis and biological evaluation of covalent peptidomimetic 3CL protease inhibitors containing nitrile moiety

In this paper, a series of peptidomimetic SARS-CoV-2 3CL protease inhibitors with new P2 and P4 positions were synthesized and evaluated. Among these compounds, 1a and 2b exhibited obvious 3CLpro inhibitory activities with IC50 of 18.06 nM and 22.42 nM, respectively. 1a and 2b also showed excellent antiviral activities against SARS-CoV-2 in vitro with EC50 of 313.0 nM and 170.2 nM, respectively, the antiviral activities of 1a and 2b were 2- and 4-fold better than that of nirmatrelvir, respectively. In vitro studies revealed that these two compounds had no significant cytotoxicity. Further metabolic stability tests and pharmacokinetic studies showed that the metabolic stability of 1a and 2b in liver microsomes was significantly improved, and 2b had similar pharmacokinetic parameters to that of nirmatrelvir in mice.

PO-03-014 LONG-TERM OUTCOMES OF CONDUCTION SYSTEM PACING IN LEFT VENTRICULAR DYSFUNCTION USING STYLET-DRIVEN LEADS: A COMPARISON WITH LUMEN-LESS LEADS

Long-term outcomes of conduction system pacing (CSP) using stylet-driven leads (SDL) among patients with left ventricular systolic dysfunction (LVSD) have neither been studied nor been compared with lumen-less leads (LLL). The study’s objective was to compare stylet-driven (SDL) leads with lumen-less (LLL) in terms of feasibility, pacing characteristics, and long-term outcomes. Retrospective analysis of single-centre data of consecutive patients with LVEF < 50% undergoing a successful CSP implant using SDLs and LLLs between Jan 2020 to Aug 2022. Indications for CSP implant were Resynchronization therapy, bradycardia indication with LVEF < 50% with anticipated to require > 20% ventricular pacing and Pacing-induced Cardiomyopathy (PICMP). Among 114 patients (age 62.2 ± 11.3 years, 65 males) with LVEF < 50% (mean 29.2 ± 7.7%) undergoing CSP, LLLs and SDLs were used in 65 and 49 patients respectively. 9 LLL and 7 SDL implants used HBP; the remaining implants were LBBAP or LOT-CRT. CRT, bradyarrhythmia with LV dysfunction, and PICMP were the indications for CSP in 77, 32, and 5 patients, respectively. CRT indication (n = 70) was treated with LLL-LBBAP/LOT-CRT, SDL-LBBAP/LOT-CRT, LLL-HBP, and SDL-HBP, respectively. LLL-LBBAP/LOT-CRT, SDL-LBBAP/LOT-CRT, LLL-HBP, and SDL-HBP were used in 8, 13, 5, and 6 patients with bradyarrhythmia-CSP, respectively. At a median follow-up of 494 days (IQR: 208–636) for LLLs and 235 days (IQR: 122–333) for SDLs, LBBAP, unlike HBP, had similar lead parameters for both LLLs and SDLs with few complications. Four fatalities (three non-cardiac) occurred among the 112 patients accessible for follow-up. QRS narrowing (LLL: 168.8 ± 15.7 to 114.3 ± 16.5 ms, SDL: 166.3 ± 16.7 to 115.0 ± 14.2 ms), LVEF improvement (LLL: 26.7 ± 6 to 48.4 ± 8.0%, SDL: 25.7 ± 6.7 to 46.7 ± 8.0%), and NYHA class improvement (LLL: 2.8 ± 0.9 to 1.1 ± 0.3, SDL: 2.6 ± 0.8 to 1.2 ± 0.4) were similar between LLLs and SDLs for patients with LBBB undergoing LBBAP/LOT-CRT. Paced QRS duration (LLL:126.3 ± 14.2 ms, SDL:120.5 ± 7.5 ms) and LVEF stabilization (LLL:34.6 ± 6 to 40.1 ± 10.1%, SDL: 38.2 ± 7.5 to 45.8 ± 7%) was comparable across LLLs and SDLs for patients undergoing LBBAP/LOT-CRT for pacing. In this largest series of SDLs in LV dysfunction and the only direct comparison of SDLs vs. LLLs among patients with LVSD, both leads showed equal and good long-term performance when used for conduction system pacing.

Modeling and Model Verification of the Stress-Strain State of Reinforced Polymer Concrete.

This article considers the prospects of the application of building structures made of polymer concrete composites on the basis of strength analysis. The issues of application and structure of polymer-concrete mixtures are considered. Features of the stress-strain state of normal sections of polymer concrete beams are revealed. The dependence between the stresses and relative deformations of rubber polymer concretes and beams containing reinforcement frame and fiber reinforcement has been determined. The main direction of the study was the choice of ways to increase the strength characteristics of concrete with the addition of a polymer base and to increase the reliability of structures in general. The paper presents the results of experimental and mathematical studies of the stress-strain state and strength, as well as deflections of reinforced rubber-polymer beams. The peculiarities of fracture of reinforced rubber-polymer beams along their sections have been revealed according to the results of the experiment. The peculiarities of fracture formation of reinforced rubber-polymer beams have also been revealed. The conducted work has shown that the share of longitudinal reinforcement and the height of the fibrous reinforcement zone are the main factors. These reasons determine the characteristics of the strength of the beams and their resistance to destructive influences. The importance and scientific novelty of the work are the identified features of the stress-strain state of normal sections of rubber-concrete beams, namely, it has been established that the ultimate strength in axial compression and tension, deformations corresponding to the ultimate strength for rubber concrete exceed similar parameters for cement concrete 2.5-6.5 times. In the case of the addition of fiber reinforcement, this increase becomes, respectively, 3.0-7.5 times.

Evaluation of derivative in damping in the Newtonian limit for non-planar wedge

The current work derives the analytical expression for damping derivative of a non planar wedge when γ tends to one and Mach number tends to infinity. Ghosh’s developed strip theory is utilized to derive the expression of damping derivative. With regard to a variety of geometrical and flow characteristics, the current theory can forecast the damping derivatives of a non planar wedge. Prior to performing exhaustive calculations and trial research, it is vital to know about these damping derivatives in order to freeze and arrive at the geometrical and kinematic similarity parameters. The ongoing technique, which is exceptionally useful during the plan stage, predicts the damping subordinates in pitch for a flat wedge effortlessly. In the Newtonian limit, the equations derived for stability derivatives become precise. The pivot position is found to influence the damping derivative directly.Additionally, it has been noted that at high angles of attack, the centre of pressure shifts significantly from the leading edge to the trailing edge. Consequently, according to the viewpoint of stability, this behavior may be utilized to stabilize the aeronautical vehicle. Therefore, in this case, the expression for the damping derivative is non-linear, the findings have been affected accordingly. However, the behaviour is linear up to a fifteen degree angle of attack before the pattern becomes non-linear.

A framework for crossover of scaling law as a self-similar solution: dynamical impact of viscoelastic board.

In this paper, a new framework for crossover of scaling law is proposed: a crossover of scaling law can be described by a self-similar solution. A crossover emerges as a result of the interference from similarity parameters of the higher class of the self-similarity. This framework was verified for the dynamical impact of a solid sphere onto a viscoelastic board. All the physical factors including the size of spheres and the impact of velocity are successfully summarized using primal dimensionless numbers which construct a self-similar solution of the second kind, which represents the balance between dynamical elements involved in the problem. The self-similar solution gives two different scaling laws by the perturbation method describing the crossover. These theoretical predictions are compared with experimental results to show good agreement. It was suggested that a hierarchical structure of similarity plays a fundamental role in crossover, which offers a fundamental insight into self-similarity in general.

Data-driven characterization of walking after a spinal cord injury using inertial sensors

BackgroundAn incomplete spinal cord injury (SCI) refers to remaining sensorimotor function below the injury with the possibility for the patient to regain walking abilities. However, these patients often suffer from diverse gait deficits, which are not objectively assessed in the current clinical routine. Wearable inertial sensors are a promising tool to capture gait patterns objectively and started to gain ground for other neurological disorders such as stroke, multiple sclerosis, and Parkinson’s disease. In this work, we present a data-driven approach to assess walking for SCI patients based on sensor-derived outcome measures. We aimed to (i) characterize their walking pattern in more depth by identifying groups with similar walking characteristics and (ii) use sensor-derived gait parameters as predictors for future walking capacity.MethodsThe dataset analyzed consisted of 66 SCI patients and 20 healthy controls performing a standardized gait test, namely the 6-min walking test (6MWT), while wearing a sparse sensor setup of one sensor attached to each ankle. A data-driven approach has been followed using statistical methods and machine learning models to identify relevant and non-redundant gait parameters.ResultsClustering resulted in 4 groups of patients that were compared to each other and to the healthy controls. The clusters did differ in terms of their average walking speed but also in terms of more qualitative gait parameters such as variability or parameters indicating compensatory movements. Further, using longitudinal data from a subset of patients that performed the 6MWT several times during their rehabilitation, a prediction model has been trained to estimate whether the patient’s walking speed will improve significantly in the future. Including sensor-derived gait parameters as inputs for the prediction model resulted in an accuracy of 80%, which is a considerable improvement of 10% compared to using only the days since injury, the present 6MWT distance, and the days until the next 6MWT as predictors.ConclusionsIn summary, the work presented proves that sensor-derived gait parameters provide additional information on walking characteristics and thus are beneficial to complement clinical walking assessments of SCI patients. This work is a step towards a more deficit-oriented therapy and paves the way for better rehabilitation outcome predictions.

Dihydropyrimidinase from Saccharomyces kluyveri can hydrolyse polyamides.

In Saccharomyces kluyveri, dihydropyrimidinase (DHPaseSK) is involved in the pyrimidine degradation pathway, which includes the reversible ring cleavage between nitrogen 3 and carbon 4 of 5,6-dihydrouracil. In this study, DPHaseSK was successfully cloned and expressed in E. coli BL-21 Gold (DE3) with and without affinity tags. Thereby, the Strep-tag enabled fastest purification and highest specific activity (9.5 ± 0.5U/mg). The biochemically characterized DHPaseSK_Strep had similar kinetic parameters (Kcat/Km) on 5,6-dihydrouracil (DHU) and para-nitroacetanilide respectively, with 7,229 and 4060M-1s-1. The hydrolytic ability of DHPaseSK_Strep to polyamides (PA) was tested on PA consisting of monomers with different chain length (PA-6, PA-6,6, PA-4,6, PA-4,10 and PA-12). According to LC-MS/TOF analysis, DHPaseSK_Strep showed a preference for films containing the shorter chain monomers (e.g., PA-4,6). In contrast, an amidase from Nocardia farcinica (NFpolyA) showed some preference for PA consisting of longer chain monomers. In conclusion, in this work DHPaseSK_Strep was demonstrated to be able to cleave amide bonds in synthetic polymers, which can be an important basis for development of functionalization and recycling processes for polyamide containing materials.

Stent appearance in a novel silicon-based photon-counting CT prototype: ex vivo phantom study in head-to-head comparison with conventional energy-integrating CT

BackgroundIn this study, stent appearance in a novel silicon-based photon-counting computed tomography (Si-PCCT) prototype was compared with a conventional energy-integrating detector CT (EIDCT) system.MethodsAn ex vivo phantom was created, consisting of a 2% agar-water mixture, in which human-resected and stented arteries were individually embedded. Using similar technique parameters, helical scan data was acquired using a novel prototype Si-PCCT and a conventional EIDCT system at a volumetric CT dose index (CTDIvol) of 9 mGy. Reconstructions were made at 502 and 1502 mm2 field-of-views (FOVs) using a bone kernel and adaptive statistical iterative reconstruction with 0% blending. Using a 5-point Likert scale, reader evaluations were performed on stent appearance, blooming and inter-stent visibility. Quantitative image analysis was performed on stent diameter accuracy, blooming and inter-stent distinction. Qualitative and quantitative differences between Si-PCCT and EIDCT systems were tested with a Wilcoxon signed-rank test and a paired samples t-test, respectively. Inter- and intra-reader agreement was assessed using the intraclass correlation coefficient (ICC).ResultsQualitatively, Si-PCCT images were rated higher than EIDCT images at 150-mm FOV, based on stent appearance (p = 0.026) and blooming (p = 0.015), with a moderate inter- (ICC = 0.50) and intra-reader (ICC = 0.60) agreement. Quantitatively, Si-PCCT yielded more accurate diameter measurements (p = 0.001), reduced blooming (p < 0.001) and improved inter-stent distinction (p < 0.001). Similar trends were observed for the images reconstructed at 50-mm FOV.ConclusionsWhen compared to EIDCT, the improved spatial resolution of Si-PCCT yields enhanced stent appearance, more accurate diameter measurements, reduced blooming and improved inter-stent distinction.Key points• This study evaluated stent appearance in a novel silicon-based photon-counting computed tomography (Si-PCCT) prototype.• Compared to standard CT, Si-PCCT resulted in more accurate stent diameter measurements.• Si-PCCT also reduced blooming artefacts and improved inter-stent visibility.

Scallion Peel Mediated Synthesis of Zinc Oxide Nanoparticles and Their Applications as Nano fertilizer and Photocatalyst for Removal of Organic Pollutants from Wastewater

Nanotechnology and nanomaterials have gained much attention in recent years due to their remarkable features. Among nanoparticles, photocatalytic material, such as zinc oxide, have shown tremendous applications in each and every field of science. In the present research, investigators have synthesized zinc oxide nanoparticles (ZnONPs) using Scallion’s peel extract. ZnONPs were both spherical and rod-shaped, where the size for spherical particles was 40–100 nm and rod-shaped, particles size was more than 200 nm as confirmed by microscopic techniques. The typical trademark bands of ZnONPs at 400–800 cm−1 were revealed by infrared spectroscopy, which also showed bands of carbonyl and hydroxyl groups. The hydrodynamic size by particle size analyzer (PSA) shows a size near about 200 nm in diameter. Furthermore, the synthesized ZnONPs were used to assess their potential as a micronutrient for the plant and nano adsorbent for the removal of antibiotics (ampicillin) and methylene blue dye from the simulated wastewater. The antibiotic and dye removal were observed under UV light and visible light against contact time. In comparison to control seeds, seeds grown with ZnONPs have shown better germination and seedling. It could be concluded that ZnONPs acted as an important nanosized source of nutrition for agricultural applications. Thus, the effect of ZnONPs has been proven as a nano-based nutrient source for agricultural purposes. The remediation study found that remediation of both ampicillin and methylene blue dye was efficient under UV light under similar experimental parameters from the simulated wastewater by the ZnONPs.

The Dynamic Interaction of Surfactants with Colloidal Molybdenum Disulfide Nanosheets Calls for Thermodynamic Stabilization by Solvents.

Top-down liquid-phase exfoliation (LPE) and bottom-up hot-injection synthesis are scalable methods to produce colloids of two-dimensional (2D) van der Waals (vdW) solids. Generally thought off as two entirely different fields, we show that similar stabilization mechanisms apply to colloids of molybdenum disulfide (MoS2) produced by both methods. By screening the colloidal stability of MoS2 produced in a hot-injection synthesis in a wide range of solvents, we observe that colloidal stability can be understood based on solution thermodynamics, wherein matching the solubility parameter of solvent and nanomaterial maximizes colloidal stability. Identical to MoS2 produced through LPE, optimal solvents to disperse MoS2 produced from the bottom-up have similar solubility parameters of ≈22 MPa1/2 and include aromatic solvents with polar functionalities, such as o-dichlorobenzene, and polar aprotic solvents, such as N,N-dimethylformamide. We further complemented our findings by nuclear magnetic resonance (NMR) spectrscopy, highlighting that organic surfactants, such as oleylamine and oleic acid, have a minimal affinity toward the nanocrystal surface and engage in a highly dynamic adsorption/desorption equilibrium. We thus conclude that hot injection yields MoS2 colloids with comparable surfaces as those produced by LPE. These similarities might offer the prospect of using established procedures developed for LPE nanomaterials to postprocess colloidally synthesized dispersions of 2D colloids as processable inks.

Application of a scoring system in Japanese patients diagnosed with atypical hemolytic uremic syndrome to assess the relationship between the score and clinical responses to eculizumab

BackgroundAtypical hemolytic uremic syndrome (aHUS) is caused by complement dysregulation and is generally diagnosed by exclusion from other disorders of thrombotic microangiopathy (TMA). Eculizumab, a terminal complement inhibitor, has been approved for aHUS treatment since 2013 in Japan. Recently, a scoring system was published to support diagnosis of aHUS. Herein we modified this scoring system to apply it to patients diagnosed with aHUS and treated with eculizumab, and assessed the association between the score and clinical responses to eculizumab.MethodsOne hundred eighty-eight Japanese patients who were clinically diagnosed with aHUS, treated with eculizumab, and enrolled in post-marketing surveillance (PMS) were included in this analysis. Some of parameters in the original scoring system were replaced with clinically similar parameters collected in the PMS to modify the system, hereafter referred to as the TMA/aHUS score, which ranges from -15 to 20 points. Treatment responses within 90 days after eculizumab initiation were also assessed, and the relationship between treatment response and TMA/aHUS scores calculated at TMA onset was explored.ResultsThe median (range) TMA/aHUS score was 10 (3–16). Receiver operating characteristic curve analysis showed that the cutoff value of TMA/aHUS score to predict treatment response to eculizumab was estimated as 10, and negative predictive value indicated that ≥ 5 points was appropriate to consider assessing the treatment response to eculizumab; 185 (98%) patients had ≥ 5 points and 3 (2%) had < 5 points. Among the patients with ≥ 5 points, 96.1% showed partial response and 31.1% showed complete response. One of the three patients with < 5 points met partial response criteria. No significant difference in the TMA/aHUS scores was observed between survivors and non-survivors, suggesting that the score was not appropriate to predict the outcome (i.e., survival/death) in patients treated with eculizumab.ConclusionAlmost all patients clinically diagnosed with aHUS scored ≥ 5 points and responded to eculizumab. The TMA/aHUS score system could become a supporting tool for the clinical diagnosis of aHUS and probability of response to treatment with a C5 inhibitor.Trial registrationThis study was conducted as per good PMS practice guidelines for drugs (MHLW Ministerial Ordinance No. 171 of 2004).

Demonstration of a superluminal laser using electromagnetically induced transparency in Raman gain.

We report the realization of a superluminal laser in which the dip in the gain profile necessary for anomalous dispersion is produced via electromagnetically induced transparency caused by the optical pumping laser. This laser also creates the ground state population inversion necessary for generating Raman gain. Compared to a conventional Raman laser with similar operating parameters but without the dip in the gain profile, the spectral sensitivity of this approach is explicitly demonstrated to be enhanced by a factor of ∼12.7. Compared to an empty cavity, the peak value of the sensitivity enhancement factor under optimal operation parameters is inferred to be ∼360.

Strength, stiffness and crack resistance of a lightweight slab structure model with spherical void formers

Precast concrete has been used for many decades with optimized consumption of concrete and reinforcement. The use of monolithic reinforced concrete in the Russian Federation cannot be called balanced in terms of its material consumption. One of the design ideas for creating lightweight slab reinforced concrete structures in monolithic construction is the BubbleDeck technology. Spherical bushings are located in places of the least stresses in the structure, which leads to a significant reduction in the own weight. The article presents the results of assessing the strength, stiffness and crack resistance of a reduced model of a reinforced concrete slab in comparison with similar parameters of a solid section slab.

Reflections on TRP and TP/GFR in the definition of renal phosphate loss: conceptual review.

Fractional tubular reabsorption of phosphate (TRP) has been used for over 60years to establish the existence of renal phosphate loss. It is a parameter of corrected volume per decilitre of glomerular filtration rate (GFR). Later, a mass parameter per dl GFR called TP/GFR (tubular PO4 reabsorption per dl GFR) was devised which some authors have sought to substitute for TRP. The aim of the present work is to attempt to demonstrate that TRP and TP/GFR are similar parameters and, in certain aspects, TRP is more effective for diagnosis. Data were gathered on the metabolism of phosphate corresponding to a group of healthy children without hypophosphatemia (n = 47), a group of patients with idiopathic hypercalciuria (n = 27), and ten patients diagnosed with X-linked hypophosphatemia (XLH). The TRP, the TP/GFR, and the percent tubular reabsorption of phosphate were calculated. All the patients with XLH presented TRP values lower than 95ml/dl GFR and of TP/GFR equal to or lower than 2.8mg/dl GFR. In the total sample, a direct correlation was observed between TRP and TP/GFR (r = 0.65; p = 0.01). The TRP and the percent tubular reabsorption of phosphate values were the same in the three groups (r = 1; p = 0.01). TRP and TP/GFR are similar parameters. TRP is more effective than TP/GFR given that in renal hypophosphatemia it is always below 95% and above 95% in reduced phosphatemia and normal kidney proximal tubular function. There is no solid reason for using TP/GFR rather than TRP. A higher resolution version of the Graphical abstract is available as Supplementary information.

INVESTIGATION OF CELL AGGREGATE SIZE AND POTENTIAL BONE GROWTH WITH BONE CELL LINE AND HUMAN MESENCHYMAL STEM CELLS

Our previous research has demonstrated that minor adjustments to in vitro cellular aggregation parameters, i.e. alterations to aggregate size, can influence temporal and spatial mineral depositions within maturing bone cell nodules. What remains unclear, however, is how aggregate size might affect mineralisation within said nodules over long-term in vivo culture.In this study, we used an osteoblast cell line, MLO-A5, and a primary cell culture, mesenchymal stem cells (MSC), to compare small (approximately 80 µm) with large (approximately 220 µm) cellular aggregates for potential bone nodule development after 8 weeks of culturing in a mouse model (n = 4 each group). In total, 30 chambers were implanted into the intra-peritoneal cavity of 20 male, immunocompromised mice (MF1-Nu/Nu, 4 – 5 weeks old). Nine small or three large aggregates were used per chamber. Neoveil mesh was seeded directly with 2 × 103 cells for monolayer control. At 8 weeks, the animals were euthanised and chambers fixed with formalin. Aggregate integrity and extracellular material growth were assessed via light microscopy and the potential mineralisation was assessed via micro-CT.Many large aggregates appeared to disintegrate, whilst the small aggregates maintained their form and produced additional extracellular material with increased sizes. Both MLO-A5 cells and MSC cells saw similar results. Interestingly, however, the MSCs were also seen to produce a significantly higher volume of dense material compared to the MLO-A5 cells from micro-CT analysis.Overall, a critical cell aggregate size appeared to exist balancing optimal tissue growth with oxygen diffusion, and cell source may influence differentiation pathway despite similar experimental parameters. The MSCs, for example, were likely producing bone via the endochondral ossification pathway, whilst the matured bone cells, MLO-A5 cells, were likely producing bone via the intramembranous ossification pathway.

Identification of sex characteristics of obesity and hypertension in the adult population of the Vladimir region

Aim. To identify sex characteristics of the prevalence of obesity and hypertension (HTN) in the adult population of the Vladimir region.Material and methods. The study included 1350 people, men and women aged 30-69 years from 5 cities of the Vladimir region (Vladimir, Kovrov, Murom, Yuriev-Polsky and Vyazniki). The response rate was at least 80%. In total, 1174 people completed the study, including 424 men (36,1%) and 750 women (63,9%). The survey was conducted according to a standard questionnaire, including socio-demographic parameters and behavioral risk factors. In all patients, anthropometric parameters, blood pressure, heart rate and resting electrocardiography were assessed.Results. The average body mass index among women was significantly highercompared to men (29,9±6,2 kg/m2 vs 28,3±4,7 kg/m2, p&lt;0,001), while waist circumference among men exceeded similar parameters among women (95,5±11,7 cm vs 91,9±15,3 cm, p&lt;0,001). Sex differences in anthropometric indicators have a number of features in different cities. Every third man is obese, while among women this figure was significantly higher (33,7% vs 42,1%, p&lt;0,001). Every second man and 2/3 of women had abdominal obesity (53,1% and 71,6%, p&lt;0,001). The prevalence of HTN among men and women was comparable (41,5% and 39,9%, respectively). Electrocardiographic signs of left ventricular (LV) hypertrophy are detected in every third respondent, while its prevalence between men and women did not differ statistically (30,5% and 35,5%, respectively).Conclusion. In the surveyed population of men and women of the Vladimir region, the prevalence of obesity and HTN were comparable. The prevalence of obesity and abdominal obesity has sex differences due to the greater severity among women. Hypertension is also highly likely to be associated with LV hypertrophy. Obviously, in order to correct obesity and HTN, lifestyle changes at the population level must be combined with the use of antihypertensive therapy to achieve target blood pressure levels and regress LV hypertrophy.

Object detection in hospital facilities: A comprehensive dataset and performance evaluation

Detecting objects in hospital indoor environments is critical for scene understanding and can have various applications in healthcare. Deep learning algorithms have proven to be effective in object recognition from images or videos, but the availability of annotated datasets plays a crucial role in their successful application. However, there is a shortage of datasets for object detection in hospital settings, hindering the advancement of hospital indoor object detection algorithms. In this paper, we present the Hospital Indoor Object Detection (HIOD) dataset, consisting of 4,417 images covering 56 object categories. The HIOD dataset represents the frequently encountered objects in hospitals and comprises 51,869 annotated objects. The dataset is characterized by dense annotation, with an average of 11.7 objects and 6.8 object categories per image. An object detection benchmark was established using the HIOD dataset and eight state-of-the-art object detectors. The benchmark provides a comprehensive evaluation of the performance of the selected object detectors on a large and diverse set of images of objects commonly seen in hospital environments. The results of the benchmark can be used to compare and analyze the performance of different object detectors and identify their strengths and weaknesses for use in hospital environments. In the benchmark, one-stage detectors have shown superior performance compared to two-stage detectors of similar parameter sizes. In particular, YOLOv6-L was able to attain a mean Average Precision (mAP) of 51.7% while operating at a detection speed of 255 FPS. The benchmark and dataset can serve as a valuable resource for researchers and practitioners in the field of computer vision and robotics, helping to advance the development of more effective and efficient object detection algorithms for developing automated operations in hospitals such as robotic disinfection and patient assistance.

Сomparison of biomechanical properties of non-absorbable and partially absorbable mesh implants used in surgery for urinary incontinence and genital prolapse: experimental study

Introduction. The amount of non-absorbable synthetic material used in the surgical treatment of pelvic organ prolapse and stress urinary incontinence has been shown to directly correlate with the risks of mesh-associated complications. Previously, we developed a partially absorbable implant. Currently, there are insufficient data on the use of partially absorbable implants in female pelvic floor reconstruction, which requires comparative biomechanical and pathomorphological studies.Objective. To compare the biomechanical properties of a new generation partially absorbable implants and nonabsorbable polypropylene mesh implants during animal model.Materials &amp; methods. Thirty-nine rabbits weighing 3200 ± 250 g were randomised into three groups depending on the implanted endoprosthesis: group 1 (n = 15) — “Axilen” implant (polydioxanone 98%, polypropylene 2%), group 2 (n = 15) — “Axilen Rapid” implant (polyglycapron 98%, polypropylene 2%), group 3 (n = 9) — “Urosling 1” implant. After implantation, rabbits of all 3 groups were divided into 3 subgroups according to the withdrawal period from the experiment: 14 days, 60 days, and 180 days. The biomaterial obtained was subjected to two studies: evaluation of the macroscopic view of the explanted mesh endoprosthesis with the formed capsule and the surrounding tissues, study of the biomechanical properties of the formed connective tissue capsule with the implant.Results. According to macroscopic evaluation, there was a less significant tissue reaction to partially absorbed implants. A comparative analysis of biomechanical indices revealed no statistically significant difference in rupture elongation at all follow-up periods among the presented groups. Tensile strength, at 60 and 180 days, was not statistically different between the groups described. At 14 days, there was a statistically significant difference in tensile strength between groups 1 and 3 (p = 0.003).Conclusion. During biomechanical animal experiments, the connective tissue capsule formed on the partially absorbable implant was not inferior to the similar parameter of the non-absorbable sample in its tensile strength, which explains a great potential for the use of partially absorbable implants in reconstructive surgery of pelvic organ prolapse and stress urinary incontinence.

N-(benzazol-2-yl)-2-substituted phenylacetamide derivatives: Design, synthesis and biological evaluation against MCF7 breast cancer cell line

This work describes the straightforward and efficient one-pot synthesis of a new library of N-(benzazol-2-yl)-2-substituted phenylacetamide derivatives (19–27). Using the MTT assay, these compounds were evaluated for their in vitro anticancer activity against the MCF7 human breast cancer cell line, and the results were compared to the standard doxorubicin. The majority of compounds exhibited an inhibitory effect against the cancer cell line, with compounds 19, 22, and 26 exhibiting exceptional cytotoxicity against MCF7 cells. Using flow cytometry, the most potent compound 19 on the induction of apoptosis in the breast cancer cell line was determined. Compound 19 induced G1-phase cell cycle arrest followed by apoptotic cell death. In silico analyses of potent compounds 19, 22, and 26 were conducted to investigate their interactions with Human DNA topoisomerase II. The energy calculations were found to be in excellent agreement with the calculated IC50 values. In addition, drug similarity parameter values for the three active compounds were determined using in silico ADME prediction studies. Considering all of these results, it appears that these N-(benzazol-2-yl)-2-substituted phenylacetamide derivatives may be effective anticancer agents. This work may possibly generate new concepts for the enhancement of inhibitors of human DNA topoisomerase II for breast cancer treatment.

Abstract 5368: AI-enabled prediction of lung cancer specific hot spot gene alterations from histology images

Abstract Background: The clinical intervention relies on molecular testing of primary biomarkers. We developed an AI enabled platform to predict NCCN guidelines recommended hot-spot genomic alterations from the image analysis using H&amp;E slide. Methods: AI engine was trained using clinically observed hot spot genomic alterations present in lung cancer patients as a ground truth based on histological FFPE whole-slide images. The H &amp; E stained histological images of the tumor having matching pathogenic mutations were downloaded from the TCGA database for the training and testing purpose. The program extracted histopathological features, followed by pattern mapping to predict genomics status based on the results of NGS testing from 480 samples. ResNet AI algorithm was trained to predict lung specific hot spot genomic alterations. An H &amp; E stained slide was imaged with a 40x objective lens to obtain a brightfield image as an input data. The input image was segmented into 65 x 103 tiles, each of 64 µm2 in size for an individual pathological class. 80% of the data set was used for training the engine while validation and blinded study was respectively performed on remaining 10% data. Results: Using single blinded clinical samples. AI model analyzed whole slides. Table 1 summarize parameters for clinically significant variants. Higher sensitivity and specificity was observed for variants encoding mutated cell surface proteins compared to variants encoding intracellular mutant targets when compared on similar training and testing parameters. Conclusions: AI-enabled prediction of hotspot genomic alterations resulted comparable to NGS with shortest TAT, cost and resources. It predicted actionable gene alterations with high positive and negative prediction values. Table 1. Gene Sample (n = 480) Evaluation parameters for tested samples Training Testing Sensitivity (%) Specificity (%) PPV (%) NPV (%) F1-score (%) Accuracy (%) KRAS 248 62 100 94 94 100 97 97 ALK 16 4 100 100 100 100 100 100 EGFR 72 18 88 100 100 90 94 94 MET 16 4 50 100 100 66 66 75 ROS 16 4 100 100 100 100 100 100 STK11 88 22 82 73 75 80 78 77 RET 24 6 50 100 100 60 50 67 BRAF 14 2 100 100 100 100 100 100 NTRK 92 24 78 56 64 71 70 67 ERBB2 12 4 50 50 50 50 50 50 Citation Format: Gowhar Shafi, Shiva Shivamurthy, Anand Ulle, Chongtham Cha Chinglemba, Sumit Haldar, Fauzul Moubeen, Mohan Uttarwar. AI-enabled prediction of lung cancer specific hot spot gene alterations from histology images. [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 5368.