Silver Oxide Nanoparticles Research Articles

Advanced Nanomaterials for Cancer Therapy: Gold, Silver, and Iron Oxide Nanoparticles in Oncological Applications.

Cancer remains one of the most challenging health issues globally, demanding innovative therapeutic approaches for effective treatment. Nanoparticles, particularly those composed of gold, silver, and iron oxide, have emerged as promising candidates for changing cancer therapy. This comprehensive review demonstrates the landscape of nanoparticle-based oncological interventions, focusing on the remarkable advancements and therapeutic potentials of gold, silver, and iron oxide nanoparticles. Gold nanoparticles have garnered significant attention for their exceptional biocompatibility, tunable surface chemistry, and distinctive optical properties, rendering them ideal candidates for various cancer diagnostic and therapeutic strategies. Silver nanoparticles, renowned for their antimicrobial properties, exhibit remarkable potential in cancer therapy through multiple mechanisms, including apoptosis induction, angiogenesis inhibition, and drug delivery enhancement. With their magnetic properties and biocompatibility, iron oxide nanoparticles offer unique cancer diagnosis and targeted therapy opportunities. This review critically examines the recent advancements in the synthesis, functionalization, and biomedical applications of these nanoparticles in cancer therapy. Moreover, the challenges are discussed, including toxicity concerns, immunogenicity, and translational barriers, and ongoing efforts to overcome these hurdles are highlighted. Finally, insights into the future directions of nanoparticle-based cancer therapy and regulatory considerations, are provided aiming to accelerate the translation of these promising technologies from bench to bedside.

Studying the properties of green synthesized silver oxide nanoparticles in the application of organic dye degradation under visible light.

In present study the green synthesis of silver oxide nanoparticles has been effectively achieved using novel plant extract Phragmanthera Macrosolen. This method provides sustainable alternative for nanoparticle synthesis, demonstrating the potential of Phragmanthera Macrosolen as a reducing and stabilizing agent in the production of Ag2O NPs. The synthesized nanoparticles were characterized for their structural, morphological, and optical properties, confirming their successful formation and potential applications in various fields. The effects of different pH values and annealing temperature of the samples on the properties of Ag2O NPs formations, as well as photo-catalytic activities towards Toluidine Blue dye degradations, were studied. Powder XRD reveals that the crystallite natures of Ag2O NPs a long with crystalline size ranges from 25.85 to 35.90nm. FIB-SEM and HR-TEM images displayed that the Ag2O NPs as spherical shapes. UV-vis spectroscopy displayed that Ag2O NPs belong to a direct-band gap of 2.1-2.6eV. FTIR- study shown that the green synthesized Ag2O NPs may be steadied via the interfaces of -OH as well as C = O groups in the carbohydrate, flavonoid, tannin, as well as phenolic acid existing in P. macrosolen L. leaf. The chemical states, electron-hole recombinations and purity of Ag and O in the synthesized Ag2O NPs were confirmed through X-ray Photoelectron Spectroscopy (XPS) and PL analysis respectively. Fascinatingly, the synthesized Ag2O NPs at pH 12 displayed high photo-catalytic degradations for TB dyes. The photo-catalytic degradations of the TB dyes were monitored spectro-photo-metrically in wave-length ranges of 200-900nm, as well as high efficiency (98.50%) with half-life of 9.5798min and kinetic rate constant of 0.07234min-1, was obtained after 45min of reactions. From this study, it can be concluded that Ag2O NPs synthesized from Phragmanthera Macrosolen aqueous extract are promising in the remediation of environmental pollution and water treatment. In this light, the study reports that Phragmanthera Macrosolen green synthesis of Ag2O NPs can effectively address environmental pollution in cost-effective, eco-friendly, and sustainable ways.

Optical, Structural and Biological Properties of Reduced Silver Oxide Nanoparticles from Anethum Graveolens Leaf Extract by Nonthermal Plasma

This research aims to develop a novel approach, employing cold plasma technology for preparing nanoparticles of silver oxide by Anethum graveolens (dill) leaf extract as a natural reducing agent. This investigation evaluated the properties of antibacterial and antibiofilm-prepared nanoparticles. Initially, dill leaf extract was prepared to stabilize and reduce the size of silver oxide nanoparticles. Improved synthesis and optimal conditions for nanoparticle formation were achieved through the application of cold plasma technology. The results obtained showed that the prepared silver oxide nanoparticles have strong antibacterial properties and that they have antibacterial activity against a different group of bacteria that cause diseases such as Klebsiella pneumoniae (K. pneumoniae), Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The results also showed the effectiveness of nanoparticles in preventing the formation of bacterial biofilms, as the highest rate of inhibition was for gram-positive bacteria S. aureus. This study demonstrated the effectiveness of plant extracts and cold plasma technology when combined in producing nanoparticles with improved properties, which may push toward the employment of these materials in developing innovative and sustainable solutions in various scientific and applied fields.

Development of advanced biopolymer thin films of Carboxymethyl cellulose, Silver, and Zinc Oxide nanoparticles for avocado fruit preservation applications

Nanomaterials in post-harvest preservation offer advantages such as non-toxicity, chemical residue-free protection, and effective inhibition of mold and bacteria. Due to their strong antimicrobial properties and their ability to form a thin nano-coating on fruit surfaces, even small amounts of nanoparticles provide extensive coverage, preventing microbial penetration. This technology significantly improves fruit quality and longevity during storage and transportation. In this work, we introduced an advanced biopolymer film of carboxymethyl cellulose (CMC) incorporating ZnO and Ag nanoparticles (NPs). Ag NPs and ZnO NPs demonstrated an outstanding antimicrobial inhibition property that can be used for fruit preservation. As a proof of concept, the biopolymer thin films enable to extend avocado ripening from up to 35 days. The sugar concentration variation, the hardness, and the weight loss of the avocado with and without the advanced biopolymer thin film integrated ZnO and Ag nanoparticles in 35 days were compared with each other to clarify the ripening reduction ability of the biopolymer thin film.

Novel plasmon-coupled Ag/Ag2O nano-particles to downregulate β-catenin pathway in triple negative breast cancer

We prepared novel plasmon-coupled silver (Ag) and silver oxide (Ag2O) nanoparticles using green synthesis and a magnetic stirrer device from lemon juice. Firstly, we prepared six Ag samples from green tea and lemon juice, characterized by XRD, FTIR, UV, and HRTEM. We selected just two samples to apply to MDA-MB-231 cells. The samples entered the cell through endocytosis, showed moderate cytotoxicity and ROS levels, caused cell growth arrest at the G2M phase, exhibited higher inhibition of Cyclin D1, and induced early apoptosis. β-Catenin is an abundant protein in triple-negative breast cancer TNBC. Both samples showed inhibition of the β-catenin proteins pathway. Plasmon-coupled nanoparticles effectively inhibited β-catenin, physically capturing β-catenin and its pathway proteins, mimicking the action of a degradation protein complex due to their geometric properties. The prepared materials could be considered a promising treatment for TNBC that has not responded to chemotherapy and radiotherapy. As they have low toxicity to normal cells; this paves the way for new material designs without strict size limitations.

Green synthesis of silver, starch, and zinc oxide mediated nanoparticles with probiotics and plant extracts, their characterization and anti-bacterial activity

Nanotechnology has various applications in all branches of science, including engineering, medicine, pharmacy, and other related fields. Conventional techniques, such as the chemical reduction approach, which produces nanoparticles (NPs) using various hazardous chemicals, offer several health risks due to their toxicity and raise serious environmental concerns. In contrast, other techniques are expensive and need a lot of energy. More than 70 % of pathogenic bacterial strains have developed resistance to at least one class of antibiotics, leading to an increase in life-threatening bacterial infections that pose a significant health risk. However, the creation of NPs by biogenic synthesis is risk-free for the environment and clean enough for biological use. This study was aimed at synthesis of novel Moringa oleifera mediated starch capped silver-zinc NPs and green synthesis of ZnO nanoparticles from Aloe vera, papaya, and Lactobacillus plantarum. Antimicrobial activity of both NPs was tested against Gram-negative antibiotic-resistant bacteria Pseudomonas aeruginosa, Gram-positive bacteria Staphylococcus aureus (ATCC 6538), and two foodborne pathogens Listeria monocytogenes and Campylobacter jejuni. Ultraviolet–visible spectroscopy, Fourier Transform Infrared Spectroscopy, and Scanning Electron Microscopy were used for characterization. Majority of the research studies stress the flexibility, repeatability, and desirable features of the metals, polymers, and plant components employed in the production of biomedical nanoparticles. Such an intuitive approach provides several advantages, particularly a reasonable total expense, compliance with healthcare and pharmaceutical implementations, and the ability to produce massive volumes for industrial use. The novelty of the presented work lies in the unusual combination of silver, starch, and zinc oxide nanoparticles using Moringa oleifera, which is an eco-friendly alternative to chemical-based methods. This research exhibits the formation of well-defined nanoparticles with strong antibacterial activity against a wide range of pathogens, giving us insights into their potential applications in various biomedical fields.

Dual-Seed Strategy for High-Performance Anode-Less All-Solid-State Batteries.

Interest in all-solid-state batteries (ASSBs), particularly the anode-less type, has grown alongside the expansion of the electric vehicle (EV) market, because they offer advantages in terms of their energy density and manufacturing cost. However, in most anode-less ASSBs, the anode is covered by a protective layer to ensure stable lithium (Li) deposition, thus requiring high temperatures to ensure adequate Li ion diffusion kinetics through the protective layer. This study proposes a dual-seed protective layer consisting of silver (Ag) and zinc oxide (ZnO) nanoparticles for sulfide-based anode-less ASSBs. This dual-seed-based protective layer not only facilitates Li diffusion via multiple lithiation pathways over a wide range of potentials, but also enhances the mechanical stability of the anode interface through the in situ formation of a Ag-Zn alloy with high ductility. The capacity retention during full-cell evaluation is 80.8% for 100 cycles when cycled at 1mA cm-2 with 3 mAh cm-2 at room temperature. The dual-seed approach provides useful insights into the design of multi-seed concepts in which, from a mechanochemical perspective, various lithiophilic materials synergistically impact upon the anode-less interface.

Development of Tetracycline by AgO Nanoparticles and Studying its Activity on Antibiotic-Resistant Bacteria

General background: Antibiotic resistance in bacteria has become a critical global health issue, necessitating the development of new strategies to enhance antibiotic efficacy. Specific background: Nanoparticles, particularly silver nanoparticles (AgNPs), have emerged as potential enhancers of antibiotics due to their unique properties and interactions with bacterial cells. Knowledge gap: However, the combination of nanoparticles with existing antibiotics, such as tetracycline, and their impact on bacterial inhibition and safety has not been fully explored. Aims: This study aims to investigate the antibacterial activity of silver oxide nanoparticles (AgO NPs) combined with tetracycline (TCS) and evaluate their effectiveness against resistant bacterial strains. Results: AgO NPs were synthesized using a photodeposition method, yielding nanoparticles with an average diameter of 2.24 nm. The AgO NPs + TCS combination demonstrated superior antibacterial activity, with a minimum inhibitory concentration (MIC) of 16 μg/mL against Staphylococci and 32 μg/mL against Pseudomonas aeruginosa, significantly outperforming standard tetracycline. Hemolysis assays confirmed the safety of the synthesized compound at all concentrations. Novelty: Silver oxide nanoparticles and tetracycline exhibit a unique synergistic interaction, enhancing antimicrobial effects by increasing bacterial membrane permeability, facilitating greater antibiotic infiltration. Implications: These findings suggest that AgO NPs combined with tetracycline offer a promising solution to overcome bacterial resistance, providing a potent and safe alternative to conventional antibiotic treatments. Highlights: AgO NPs and tetracycline show enhanced antibacterial effects. More effective than standard tetracycline against resistant bacteria. Safe with no toxicity observed in hemolysis tests. Keywords: Antibiotic resistance, silver nanoparticles, tetracycline, photodeposition, antibacterial activity

Silver Oxide Nanoparticle Decorated Carbon Nanotube as Efficient Electron Gun

AbstractAlthough there have been numerous great field emission reports of pure and hybrid carbon nanotubes (CNTs) with ultra‐low turn‐on field and ultra‐high emission stability, practically all of the reports indicate concern about CNT manufacture on a large scale. This is due to the fact that CNT experiments require high pressure, temperature, metal catalysis, and an inert environment, and even after meeting all of these perfect conditions, the yield is quite low. Furthermore, as CNT is relatively inert in nature, it is nearly hard to make it reactive with any other metal without adequate functionalization. Keeping this in mind, this work reports the synthesis of CNT in amorphous form by a simple low‐temperature solid‐state reaction between ammonium chloride and ferrocene. The as‐synthesized CNT is further functionalized by silver oxide nanoparticle without additional functionalization. X‐ray diffraction (XRD) confirms the proper phase formation as well as the successful functionalization of the pure and hybrid sample, electron microscopic images confirm the successful functionalization of the as‐prepared CNT whereas Fourier transformed infrared (FTIR) spectroscopic analysis gives the ideas about different bonding present in all the samples. It has been found that the hybrid sample gives much better field emission performance compare to the pure CNT with betterment in both turn‐on field and enhancement factor more than 100%. The betterment is believed to be due to the favorable band bending, larger number of emission sites, and sharp curvature of the silver particles.

The influence of Ag2O/NbO2 nanocomposites on the optical properties of PVA/PVP lend in biomedical applications.

To study the optical properties of nanocomposites as well as antibacterial activity. The experimental study was conducted at the University of Babylon, College of Science and College of Education for Pure Sciences, Babylon, Iraq, from September 2021 to February 2022. Impregnation of transparent matrix polyvinyl alcohol and polyvinyl pyrrolidone nanocomposites was done by silver oxide and niobium oxide nanoparticles. The nanostructures were created using different ratios of polymer matrix, silver oxide nanoparticles and niobium oxide nanoparticles. The optical features of these nanocomposites were examined, whereby the latter type of properties was tested within the wavelength range of 220-820nm. The determination of the anti-microbial activity was done by disc diffusion method. The anti-bacterial activity involved gram-positive and gram-negative organisms. Different bacteria were cultured with Muller-Hinton medium. Absorbance, absorption coefficients and optical conductivity of the nanocomposites increased with the increase in nanocomposite concentrations. The energy gap for silver oxide and niobium oxide nanoparticles decreased when the concentrations of the nanoparticles increased. Promising outcomes may be achieved for the nanocomposites in anti-bacterial applications as the inhibition zones increased along with increased ratio of silver oxide and niobium oxide nanoparticles.

Assessment of Bryophyllum pinnatum mediated Ag and ZnO nanoparticles as efficient antimicrobial and cytotoxic agent.

Bryophyllum pinnatum is used to cure infections worldwide. Although the flavonoids of this plant are well known, it is still unknown how much of the plant's Ag and ZnO nanoparticles are beneficial. In the current research work, silver and zinc oxide nanoparticles were prepared using Bryophyllum pinnatum extract. The synthesized particles were characterized by UV-visible spectroscopy, SEM, EDS, XRD and FTIR. Synthesized particles were subjected to evaluation of their bactericidal and antifungal activity at various doses. Uv vis spectra at 400nm corresponding to AgNPs confirmed their synthesis. Strong peaks in the EDS spectra of Ag and ZnO indicate the purity of the sample. The scanning electron microscopic images of ZnONPs showed a size of about 60nm ± 3nm, which demonstrated the presence of triangular-shaped ZnO nanoparticles. Green synthesized nanoparticles showed bactericidal activity against both Gram-positive (Micrococcus luteus, Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Agrobacterium tumifaciens, Salmonella setubal, Enterobacter aerogenes) strains. AgNPs proved to be more effective against Gram-negative bacterial strains compared to Gram-positive owing to MIC values (10 ppm and 20 ppm respectively). Whereas, ZnONPs were found more effective against Gram-positive bacteria with lower MIC values (10 ppm) as compared to Gram-negative ones (20 ppm). Also, the synthesized nanoparticles exhibited moderate dose-dependent antifungal activity against tested fungal strains ranging from 10 to 70%. Cytotoxicity of nanoparticles was found significant using Brine shrimp's lethality assay with IC50 values of 4.09 ppm for AgNPs, 13.72 ppm for ZnONPs, and 24.83 ppm for plant extract. Conclusively, Ag and ZnO nanoparticles were more effective than plant extract and AgNPs had higher activities than those of ZnONPs. Further research is warranted to explore the precise mechanism of action and the potential applications of these nanoparticles in the medical field.

Synthesis, structural, and optical characterizations of zinc oxide: silver oxide nanoparticles conjunction with polymer polyvinylpyrrolidone

Synthesis, structural, and optical characterizations of zinc oxide: silver oxide nanoparticles conjunction with polymer polyvinylpyrrolidone

Probing variation of the structure and electrical conductivity of functionalized PVA based composite films doped with silver nanoparticles and irradiated by gamma rays

Polyvinyl alcohol/sulfuric acid/ethanol films doped with nano-silver particles are prepared using in-situ chemical reduction method. The as-prepared films are irradiated with gamma rays at dose range 0–50 kGy. The elemental analysis of as-prepared samples using energy dispersive x-ray spectroscopy (EDXS) showed presence of oxygen and silver peaks which refers to the probable formation of silver oxide nano-particle (Ag2O NPs) phase. Moreover, scanning electron microscope (SEM) analysis confirmed that the inserted Ag nanoparticles have regular and uniformly distributed pores within the PVA network. X-ray diffraction (XRD) measurements indicated that of Ag2O and Ag nanoparticles are embedded in the PVA matrix in cubic face-centered structure. Structural variations due to gamma irradiation are also probed by high-resolution transmission electron microscopy (HR-TEM) which displayed that the diameter of encapsulated Ag nanoparticles increases from ∼15 nm up to 34 nm against irradiation dose. The measured dc electrical conductivity results indicated that the thermal activation energy decreased from 0.95 eV to 0.56 eV as a sequence of irradiation dose in the range 0–50 kGy The evaluation of the frequency exponent factor, s, derived from the measured ac conductivity data indicated that correlated barrier hopping (CBH) is the most probable conduction mechanism for the present films. The measured dielectric constants and Cole-Cole diagrams are employed in the analysis of the dependence of the dielectric constant on the irradiation dose.

A meta‑analysis assessing the cytotoxicity of nanoparticles on MCF7 breast cancer cells.

The present study summarizes the current available literature regarding the viability of MCF7 breast cancer cells treated with gold (Au), silver (Ag) or zinc oxide (ZnO) nanoparticles at varying doses for 48 h. The data for this study were obtained from diverse research articles published between 2013 and 2023 using Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines. The evaluation focused on 20 PRISMA-compliant articles concerning MCF7 cells, yielding 137 outcome measures for meta-analysis. A generalized linear mixed model meta-analysis approach was employed to glean insights into the effects of novel nanoparticles on MCF7 breast cancer cells. The analysis covered a wide range of concentrations: Ag nanoparticles from 1.25 to 1,000 µg/ml, Au nanoparticles from 50 to 150 µg/ml, and ZnO nanoparticles from 1 to 1,000 µg/ml. Both intra-nanoparticle and inter-nanoparticle comparisons were conducted to detect differences. The findings showed that when concentrations reached or exceeded 60 µg/ml, considerable variation of cell viability was observed: Treatment with Ag nanoparticles resulted in cell viability ranging from 9 to 45%, ZnO nanoparticles resulted in cell viability ranging from 20 to 40%, and Au nanoparticles resulted in cell viability ranging from 3 to 58%. These findings indicated the significance of thoroughly exploring nanoparticle dosage to acquire a comprehensive understanding of their influence on cell viability.

Green synthesized silver and zinc oxide nanoparticles using Ficus carica leaf extract and their activity on human viruses

Green synthesized silver and zinc oxide nanoparticles using Ficus carica leaf extract and their activity on human viruses

Clove mediated synthesis of Ag-ZnO doped fucoidan nanocomposites and their evaluation of antibacterial, antioxidant and cytotoxicity efficacy study

The Ag-ZnO/Fu nanocomposites were successfully synthesized in an environmentally friendly manner using an aqueous Clove buds extract from the Syzygium aromaticum plant. They were then examined for antibacterial activity against bacterium strains of Escherichia coli, Methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa, and Enterococcus faecalis. The UV–Visible spectrophotometry analysis confirmed the presence of zinc oxide (ZnO) nanoparticles at 334 nm, silver oxide (Ag) nanoparticles at 416 nm, and fucoidan at 589 nm in the synthesized nanocomposites. The FT-IR analysis confirmed the presence of five different functional linkages in the synthesized nanocomposites. The positions of diffraction peaks in pure Ag-ZnO/Fu nanocomposite patterns were identified using XRD analysis. The Ag-ZnO/Fu nanocomposites synthesized are more crystalline (77 %), and less amorphous (22.2 %). Silver, O, and Zn signals were detected in the SEM and EDX spectra of the Ag-ZnO/Fu nanocomposite. Different strains of Ag-ZnO/Fu nanocomposites showed stronger antibacterial properties. The DPPH technique was used to perform the antioxidant assay. Cytotoxicity testing was carried out on zebrafish embryos treated with several groups of Clove Ag ZnO/Fucoidan. The combination Ag-ZnO/Fucoidan (300 mg/mL) showed a marginally significant difference in survival (94 %).

A Facile and Green Approach for the Preparation of Silver Nanoparticles on Graphene Oxide with Favorable Antibacterial Activity.

Herein, we introduce a simple precipitation method for preparing graphene oxide-silver nanoparticle (GO/AgNP) composites, utilizing Calendula officinalis (C. officinalis) seed extract as both a reducing and stabilizing agent. Our research combines the sustainable preparation of graphene oxide (GO) with the green synthesis of silver nanoparticles (AgNPs), aiming to explore the potential of the obtained composite as a novel antibacterial material. To establish a benchmark, the synthesis was also performed using sodium citrate, a conventional reducing agent. The resultant GO/AgNP composites were characterized through several analytical techniques, including scanning electron microscopy (SEM), transmission electron microscopy (TEM), atomic force microscopy (AFM), energy dispersive X-ray spectroscopy (EDS), Raman spectroscopy, X-ray diffraction (XRD), infrared (IR) spectroscopy, and ultraviolet-visible (UV-vis) spectroscopy, confirming the successful functionalization of GO with AgNPs. The antibacterial effectiveness of the composites was systematically assessed against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus), with nanoparticle concentrations spanning from 0 to 250 µg/mL, utilizing mostly disk diffusion and colony-forming unit (CFU) count assays. The AgNPs were characterized by a size range of 15-50 nm. Notably, the GO/AgNP composite prepared using C. officinalis seed extract demonstrated superior antibacterial activity at all tested concentrations, outperforming both pure GO and the GO/AgNP composite prepared with sodium citrate. The most pronounced antibacterial effect was observed at a concentration of 32.0 µg/mL. Therefore, this innovative synthesis approach may offer a valuable contribution to the development of new therapeutic agents to combat bacterial infections, suggesting further exploration into antibacterial coatings or potential drug development.

P23-16 Biokinetics of carbon black, multi-walled carbon nanotubes, cerium oxide, silica, titanium dioxide, silver, gold, copper oxide and zinc oxide nanoparticles after inhalation – analysis of existing data

P23-16 Biokinetics of carbon black, multi-walled carbon nanotubes, cerium oxide, silica, titanium dioxide, silver, gold, copper oxide and zinc oxide nanoparticles after inhalation – analysis of existing data

The Impact of Orthodontic Adhesive Containing Resveratrol, Silver, and Zinc Oxide Nanoparticles on Shear Bond Strength: An In Vitro Study.

Introduction The goal of orthodontic treatment is to provide patients with esthetic smiles and functional occlusion. Despite best efforts and continuous evolution of materials, white spot lesions present a persistent hindrance to the desired treatment outcome. Nanoparticles have shown efficacy in reducing microbial activity; however, currently, there is a need for natural anti-cariogenic compounds with minimal side effects. Resveratrol is a natural compound belonging to the polyphenol group and has shown promising anti-microbial efficacy. This study aimed to evaluate the influence of dentin bonding agentsincorporated with the following three different nanoparticles on shear bond strength: silver nanoparticles (Ag-Np), zinc oxidenanoparticles (ZnO-Np), and resveratrol nanoparticles (RSV-Np). Materials and methods A total of 40 premolar teeth therapeutically extracted were assigned to four equal groups of n=10 each. Groups 1, 2, and 3 used experimental adhesivesdoped withsilver, zinc oxide, and resveratrol nanoparticles, respectively. Group 4 was bonded using unmodified adhesive. The bonded teeth were then subjected to shear bond strength (SBS) testing which was measured using a Universal Testing Machine (model no. UNITEST-10; Pune, India: ACME Engineers).Statistical analyses were performed using SPSS version 21 (Armonk, NY: IBM Corp.), employing one-way ANOVA and Tukey's post-hoc test for pairwise comparisons. Results Shear bond strength testing revealed that the control group with unmodified adhesive (8.6 MPa) had the highest SBS, followed by RSV-Np (7.6 MPa), Ag-Np (6.3 MPa), and ZnO-Np (5.65 MPa). Although the experimental groups demonstrated decreased SBS compared to the control, the values for Ag-Np and RSV-Np fell within the acceptable range. Conclusion Resveratrol nanoparticleshad the least impact on shear bond strength among the experimental groups. These findings suggest that the incorporation of resveratrol nanoparticles in dentin bonding agents can provide anti-cariogenic effect without significantly impacting the adhesive's mechanical properties thereby providing a new and promising alternative to synthetic nanoparticles. Further studies are recommended to optimize the balance between anti-microbial efficacy and bond strength in clinical applications.

A novel “cells-on-particles” cytotoxicity testing platform in vitro: design, characterization, and validation against engineered nanoparticle aerosol

The presented research introduces the "Cells-on-Particles" integrated aerosol sampling and cytotoxicity testing in vitro platform, which allows for the direct assessment of the biological effects of captured aerosol particles on a selected cell type without the need for extraction or resuspension steps. By utilizing particles with unaltered chemical and physical properties, the method enables simple and fast screening of biological effects on specific cell types, making it a promising tool for assessing the cytotoxicity of particulate matter in ambient and occupational air. Platforms fabricated from cellulose acetate (CA) and poly[ε]caprolactone (PCL) were proven to be biocompatible and promoted the attachment and growth of the human bronchial epithelial cell line BEAS-2B. The PCL platforms were exposed to simulated occupational aerosols of silver, copper, and graphene oxide nanoparticles. Each nanoparticle type exhibited different and dose-dependent cytotoxic effects on cells, evidenced by reduced cell viability and distinct, particle type-dependent gene expression patterns. Notably, copper nanoparticles were identified as the most cytotoxic, and graphene oxide the least. Comparing the “Cells-on-Particles" and submerged exposure (“Particles-on-Cells") testing strategies, BEAS-2B cells responded to selected nanoparticles in a comparable manner, suggesting the developed testing system could be proposed for further evaluation with more complex environmental aerosols. Despite limitations, including particle agglomeration and the need for more replicates to address variability, the “Cells-on-Particles” platform enables effective detection of toxicity induced by relatively low levels of nanoparticles, demonstrating good sensitivity and a relatively simpler procedure compared to standard 2D cell exposure methods.