Silver Nanoparticles In Solution Research Articles

Evaluation of fish skin as a biological dressing for metacarpal wounds in donkeys

BackgroundThe use of biological dressings has recently emerged in the management of burns and wounds. The aim of the present study was to evaluate the Nile tilapia skin as a biological dressing for full-thickness cutaneous metacarpal wounds in donkeys. The study was conducted on nine clinically healthy donkeys (n = 9). Here, fish skin dressings were obtained from fresh Nile tilapia (Oreochromis niloticus and sterilized by immersion in silver nanoparticles (AgNPs) solution for 5 min, with no change in collagen content. Bilateral, circular full-thickness excisional skin wounds (2 cm in diameter) were created on the dorsal aspect of the mid-metacarpals of each donkey. Wounds on the right metacarpals (treated wounds, n = 9) were dressed with sterile fish skins, while wounds on the left metacarpals (control wounds, n = 9) were dressed with sterile non-adherent dressing pads without any topical applications. Wound dressings were changed weekly. Wounds were evaluated microbiologically, grossly, and histologically on days 7, 14, and 21 post-wound inductions.ResultsFish skin-dressed wounds showed a significant (P < 0.0001) reduction in microbial counts (Total viable bacterial count, Staphylococcal count, and Coliform count), a significant (P < 0.0001) decrease in the wound size, and a significant reduction (P < 0.0001) in the epithelial gap compared to the untreated wounds. No frequent dressing changes were needed.ConclusionsFish skin dressing accelerated the wound healing process and efficiently inhibited the local microbial activity and exuberant granulation tissue formation suggesting its reliable and promising application for metacarpal wounds of donkeys.

Hydrodynamic Diameter of Silver Nanoparticles in Solutions of Nonionic Surfactants

Physicochemical characteristics of silver nanoparticles with known sizes are studied, depending on the concentration of nonionic surfactants and the concentration of the metal. Surfactants are used that form micelles of different sizes and different intensities of light scattering. It is shown that the surface tension and adhesion properties of solutions of nonionic surfactants are determined by the solvent in ranges of concentration lower than 1 mol/L. An approach based on spectrophotometry and viscosimetry is proposed that allows determination of the most likely reasons for discrepancies in the hydrodynamic diameter of nanoparticles in solutions of nonionic surfactants and the actual diameter of the nanoparticles.

Electrochemical synthesis of silver nanoparticles in solutions of rhamnolipid

The electrochemical method of synthesis of silver nanoparticles (AgNPs) using ‘sacrificial’ silver anode as a source of Ag+, microbial origin surfactant (rhamnolipid, RL) as a stabiliser of AgNPs and electric current as a reducer is presented. Using of cyclic voltammetry it was determined that RL concentration and temperature are the main factors influencing the rate of anodic dissolution of silver and cathodic reduction of the formed complex [AgRL]+. Increasing of RL concentration leads to increasing of the cathodic currents that indicate the increase of the rate of AgNPs formation. With increasing the temperature from 40 to 60°C the values of the anode and cathodic currents increase to 1.5 times, this indicates the predominant diffusion nature of the influence of the temperature on the synthesis of AgNPs. Obtained AgNPs were investigated using transmission electron microscopy and it was found that increasing of concentration of surfactant leads to decreasing of the size of AgNPs, whereas increasing of temperature, on the contrary, leads to increasing of mean size of AgNPs. Synthesised AgNPs showed significant antimicrobial activity against the bacterial phytopathogens Xanthomonas campestris and Agrobacterium tumefaciens. It has been shown that AgNPs stabilised by RL are characterised by low phytotoxicity.

SYNTHESIS SILVER NANOPARTICLES AND ITS APPLICATION FOR WASTEWATER TREATMENT: CATALYTIC AND PHOTOCATALYTIC DEGRADATION METHYLENE BLUE

This paper aims to propose a simple, eco-friendly method for obtaining colloidal solutions of silver nanoparticles (Ag NPs) by using of contact non-equilibrium low-temperature plasma in presents carboxymethyl cellulose (CMC) as a stabilizing agent and investigation on the decolorization of representative cationic phenothiazine dye in presence of nanocatalysts. Silver nanoparticles were prepared in aqueous AgNO3 solution by using of contact non-equilibrium low-temperature plasma in the present of carboxymethyl cellulose as capping agent. Ultraviolet–visible (UV) spectroscopy, X-ray diffraction, zeta potential, DLS analysis and SEM were used to study the formation and properties of silver nanoparticles. Measurements of optical spectra showed that the surface plasmon resonance was localized around 410-418 nm when the reaction mixture exposed to plasma for 1-5 min. The nanoparticles exhibited a good catalytic reduction for methylene blue dye in the presence of NaBH4. It followed pseudo-first order kinetics with a rate constant of 0.16 min−1. The catalytic degradation reaction was completed (98-99%) within 25 min. The photocatalytic activity of the synthesized silver nanoparticles was examined by degradation of methylene blue under sunlight irradiation. Synthesized silver nanoparticles were effectively degrading the dye nearly 98% at 70-72 h of exposure time.

Acetone sensing and modeling through functionalized Ag-catalysts and CNTs via low cost metal-organic framework-derived ZnO nanostructures

Cost-effective synthesis, portability, quick response and fascinating features of semiconducting metal oxide sensors have drawn much accredited to be utilized in wide variety of potential sensing applications. Herein, flexible acetone sensor was fabricated through spray coating method using different conductive inks of carbon nanotubes (CNTs), zinc oxide (ZnO) and silver (Ag) nanoparticles solutions on cellulose paper. Morphological analysis revealed the fiber like morphology of cellulose over which CNTs, ZnO and Ag nanoparticles are uniformly distributed. The energy dispersive X-ray spectroscopic analysis was performed to observe the elemental composition in the as-prepared samples. The recovery time toward acetone was greater for CNTs/Ag acetone sensor at 95% as compared to CNTs/ZnO/Ag sensor. Additionally, sensing time of the samples towards acetone was measured and the observed response time was greater for CNTs/ZnO/Ag (0.52 s) acetone sensor as compared to CNTs/Ag sensor (0.41 s). Hence, CNTs/ZnO/Ag can be easily employed for sensing applications due to quick response.

Nonlinear Absorption of Methylene Blue Solutions in The Presence of Plasma Nanoparticles with Various Surface Charge

The nonlinear light absorption of aqueous and alcohol solutions of methylene blue has been studied by the method of Z-scanning under continuous pumping. It is shown that the mechanisms of optical nonlinearity of these solutions vary depending on the dye concentration and type of solvent. The dependence of the amplitude of nonlinear absorption of dye molecules on the concentration and sign of the surface charge of gold and silver nanoparticles in solution is revealed. Mathematical models of optical nonlinearity for concentrated and diluted solutions of dye molecules are considered; in the latter case, the influence of plasmon nanoparticles on the nonlinear optical characteristics of methylene blue solutions is taken into account.

Silver Nanoparticles Stabilized with Chitosan Succinamide: Synthesis and Antibacterial Activity

A water-soluble chitosan derivative, chitosan succinamide, was used as a polymer matrix for the formation of silver nanoparticles via ion reduction under microwave irradiation in the presence of a D-glucose reducing agent. UV spectroscopy confirmed the presence of silver nanoparticles in the synthesized colloidal solution. Transmission electron microscopy showed that the synthesized silver nanoparticles had a spherical shape. The obtained diffractograms confirm the presence of reflexes characteristic of the crystal lattice of metallic silver. In vitro studies showed that the synthesized colloidal solution of silver nanoparticles exhibits pronounced antibacterial activity against strains of the gram-positive bacteria Bacillus subtilis ATCC 6633 and Bacillus coagulans 429.

Structural, thermal, optical and conductive properties of PAM/PVA polymer composite doped with Ag nanoparticles for electrochemical application

In the present study, the chemical preparation of silver nanoparticles (Ag NPs) solution was achieved by decreasing silver salt via the use of sodium borohydride (NaBH4). The particles appeared to have a crystalline nature through the XRD study, with a face-centered cubic (FCC) structure. The formation of silver nanoparticles (Ag NPs) was asserted by the UV, XRD and TEM. An absorption peak at around 430 nm was demonstrated through UV–visible spectrum of the aqueous medium which related to of Ag NPs. The solution casting method was used to prepare new nanocomposites films on the bases of polymer blend which related to of PAM- and PVA-doped with different wt% of Ag NPs. Various analytical techniques were used to characterize the prepared films. It was confirmed through the XRD analysis that there existed a complex formation between polymer blend and Ag NPs. A reduction in the value of the optical bandgap was displayed by UV–Vis with an increase in the concentration of Ag NPs. It was shown through the FTIR spectrum that there existed changes in the FTIR spectrum with dopant concentration indicating the interaction of dopant with the polymer blend. A great improvement in the thermal stability of the composites was proved through the TGA study with the loading of Ag NPs. The ionic conductivity at room temperature was improved by the doping of Ag NPs ions into the polymer blend system. This is attributed to the increase in mobile charge carriers and their mobility. The dielectric values in the studied frequency range indicate a strong dielectric dispersion which increases as the content of the Ag NPs increases. Consequently, these films can be suggested as a suitable application in optical devices and dielectric applications due to the observed improvement in optical properties and ac conductivity.

Synthesis of colloidal silver nanoparticles and their stabilization in several ways for external applications

Background . Today, many studies reflect an increase in the antibacterial effect of drugs through the use of a colloidal solution of silver nanoparticles. Creation of the various forms of drugs for an external use with a slow-release active component and antibacterial activity is relevant and promising for use in the treatment of superficial wounds and injuries. Objective . Synthesis and stabilization of quasispherical silver nanoparticles for external applications. Design and methods . The citrate method was used for the synthesis of colloidal silver. Dosage forms of external use were prepared by several methods: reaction with albumin, liposomal form, thickening with Aerosil and incorporation into microspheres. Results . Several methods of possible preparation of finished dosage forms based on colloidal silver nanoparticles have been demonstrated: reaction with albumin, liposomal form, thickening with Aerosil and incorporation into microspheres. Conclusion . Further investigation of both antimicrobial activity and cytotoxicity will reveal dosage forms with optimal efficiency/safety ratio.

Design of Nanosilver-Epoxy Films for Hygienic Pharmaceutical Packaging

Nanosilver-in-epoxy lining can serve as self-disinfecting coating in pharmaceutical packaging materials and surfaces. Silver nanoparticles were synthesized using antioxidant-rich fractions of the methanolic extracts of Alchornea cordifolia. The concentration ratio of extract to silver nitrate was varied (from 2:1, 1:1 to 1:4) to obtain several batches of silver nanoparticles (A to F). The resulting silver nanoparticles were characterized using UV-visible spectroscopy, photon correlation spectroscopy, and atomic force microscopy. Antimicrobial activities of the batches of silver nanoparticles were tested in vitro using selected bacteria and fungi. Different volumes of the optimized silver nanoparticles solution were mixed with epoxy resin in different ratios, resulting in film 1 (21% v/v) and film 2 (40% v/v). Square films were cut from the coated substrate and their antimicrobial inhibition, eye irritation, and other properties tested. The optimized silver nanoparticles (batch D) were obtained by reacting the extract and silver nitrate in a 1:2 ratio, and it exhibited an excellent activity against both gram-positive and gram-negative organisms as well as fungi. It also exhibited the lowest 2-month particle size and polydispersity. Films 1 and 2 differed significantly in bioactivity (P < 0.05), with film 2 demonstrating a good level of inhibition against Escherichia coli, Staphylococcus aureus, and Salmonella typhi, but not fungi. It did not elicit any irritation using the Draize eye irritation model. Silver nanoparticle-in-epoxy matrix synthesized with the extract maintains an adequate level of antibacterial activity and finds application in protecting surfaces and packages where bacterial contamination may pose a problem.

Silver nanoparticles-tragacanth gel as a green membrane for effective extraction and determination of capecitabine.

A novel eco-friendly and effective electromembrane extraction method combining high-performance liquid chromatography with UV detection was developed for the enrichment and determination of capecitabine. Tragacanth-silver nanoparticles conjugated gel was prepared by dissolving the tragacanth powder in synthesized silver nanoparticles solution and was used as a green membrane in electromembrane extraction. The porosity and presence of silver nanoparticles in the gel were characterized by field emission scanning electron microscopy. This new electromembrane extraction approachuses neither organic solvent nor carrier agents to extract the target analyte. The best electromembrane extraction efficiency was obtained by using 4.0mm membrane gel thickness containing 2.5% w/v of tragacanth gum, donor phase pH=5.0, acceptor phase pH=3.0, applied voltage 50 V, extraction time 20min, and agitation rate 500rpm. During method validation under the optimized conditions, good linearity dynamic range between 1 and 500ng/mL with the coefficient of determination (R2 )=0.998 was obtained. Limit of detection and Limit of quantitation were estimated to be 0.84 and 1.0ng/mL, respectively. Finally, the applicability of this method in real samples was confirmed by an acceptable performance in extraction and determination of capecitabine in human plasma samples.

INVESTIGATION OF ELECTROCHEMICAL PROPERTIES OF SILVER NANOPARTICLES CONJUGATED WITH ANTIBODIES TO TICK-BORNE ENCEPHALITIS FOR DEVELOPMENT OF ELECTROCHEMICAL IMMUNOSENSOR

In the past decade, interest to electrochemical assays have grown for determination antibodies in clinical and biological samples, where instead of the enzyme label metal nanoparticle is used. Such types of electrochemical immunosensors represent an upcoming trend in analytical chemistry. In this work, the electrochemical behavior of silver nanoparticles conjugated with antibodies to tick-borne encephalitis virus at different stages of electrode modification is investigated. Silver nanoparticles were obtained by chemical reduction from silver nitrate. Passive adsorption was chosen as a strategy for binding silver nanoparticles to antibodies to tick-borne encephalitis virus. The optimal ratio of antibodies to tick-borne encephalitis virus (4.5 IU ml-1) in a solution of silver nanoparticles [1 mmol l-1 AgNO3] was found experimentally. The development of an electrochemical immunosensor was based on indirect, non-competitive format of enzyme-linked immunosorbent assay. The electrochemical situation on the electrode surface after modification steps was demonstrated by cyclic voltammetry using a standard redox pair [Fe(CN)6]3-/[Fe(CN)6]4-. Glassy carbon electrode with electrochemically deposited gold nanoparticles was used as a platform for immobilization of biological material. It has been established that after sensitization of the electrode with antigen, passivation of the surface occurs. The voltammetric signal was recorded through the detection of silver ions oxidation on unmodified glassy carbon electrode under the working conditions: scan rate 0.1 V s-1, potential accumulation –0.8 V, accumulation time 60 s. Thus, this work paves the way for a new alternative method for monitoring antibodies to tick-borne encephalitis virus in biological fluids.

The Effect of Silver, Zinc Oxide, and Titanium Dioxide Nanoparticles Used as Final Irrigation Solutions on the Fracture Resistance of Root-Filled Teeth.

AimThis study was conducted to evaluate the effect of three nanoparticle solutions used as final root canal irrigants on the fracture resistance of endodontically treated roots.Materials and MethodsSixty intact single-rooted premolar teeth were used after removing the crowns below the cementum–enamel junction to standardize the length of the remaining roots to 13 mm. After instrumenting the roots using ProTaper Universal rotary instruments up to size F4, the roots were randomly divided into six groups (n=10) according to the final irrigating solutions: Group 1: normal saline; Group 2: 2% chlorhexidine (CHX); Group 3: 17% EDTA+2.5% NaOCl; Group 4: 17% EDTA+0.1% silver nanoparticle (SNP) solution; Group 5: 17% EDTA+0.1% titanium dioxide nanoparticle (TNP) solution; Group 6: 17% EDTA+0.1% zinc oxide nanoparticle (ZNP) solution. After filling the root canals with gutta-percha and AH Plus sealer, the fracture resistance (FR) values were measured. The data were subjected to the one-way analysis of variance and the Tamhane post hoc test (p=0.05).ResultsEDTA + NaOCl had the lowest FR value, followed by normal saline and CHX (p values < 0.05). No significant difference was found between the FR of roots irrigated with CHX and normal saline (p > 0.05). The application of nanoparticles (SNP, TNP, and ZNP) was associated with a significantly greater FR than that of other irrigation solutions (p values < 0.05).ConclusionThe final irrigation of root canals with nanoparticles enhanced the fracture resistance of the endodontically treated roots. The lowest FR value was observed for NaOCl.

SERS substrates based on silver nanoparticles and graphene oxide

Simple work methods for preparing active coatings for surface-enhanced Raman scattering (SERS) are demonstrated in the article. SERS active films of colloidal silver nanoparticles and composites based on graphene oxide and silver nanoparticles were obtained. The prepared disperse solutions of silver nanoparticles and graphene oxide/Ag nanoparticles composites have different stability. Using a scanning electron microscope, the structure of the prepared films was studied. A comparative characteristic of the obtained SERS active substrates is given. The dye Rhodamine 6G was used as the test substance. It was shown that the smallest enhancement of the Raman signal is produced by a coating of colloidal silver nanoparticles, while the graphene oxide/Ag nanoparticles composite obtained by the 2-stage method provides the greatest gain. The different ability to amplify the Raman signal is explained by the difference in the structure and composition of the obtained SERS active films. All prepared coatings make it easy to detect low dye concentrations (from a 10–6 M solution) both with resonance (532 nm) and nonresonance (632.8 nm) excitation.

Inhibition efficiency of silver nanoparticles solution on corrosion of mild steel, stainless steel and aluminum in 1.0 M HCl medium

Research work on corrosion behaviour of metals and their alloys continues to attract considerable attention because of the huge cost required to prevent corrosion and replace failed or contaminated components due to corrosion reactions. Different inhibitors have been used to minimize corrosion rate, but addition of nanoparticles has been reported to enhance inhibition efficiency. In this work, the influence of silver nanoparticles (AgNPs) on inhibition of corrosion of mild steel, stainless steel and aluminum in 1.0 M HCl were investigated. Biosynthesized AgNPs were acquired from Laboratory of Industrial Nanobiotechnology, Ladoke Akintola University of Technology Ogbomoso. The particles were hitherto characterized using FTIR, UV-Vis and TEM. Five concentrations of AgNPs solution (0, 5, 10, 15, and 20 μg/ml) were added to 1.0 M HCl. The corrosion inhibition effects of the AgNPs on the metal samples were analyzed using gravimetric (weight loss), potentiodynamic polarization and gasometric (hydrogen gasevolution) approaches. Gravimetric study was conducted within 2000 h of exposure, and the results showed that weight loss increased with exposure time but decreased with increased concentration of AgNPs solution. In addition, inhibition efficiency was enhanced by the addition of AgNPs solution; 52% for mild steel, 70% for stainless steel and 62% for aluminum. Gasometric results revealed that the volume of hydrogen gas evolution reduced with increased exposure time and concentration of AgNPs solution. Furthermore, potentiodynamic polarization results showed that the presence of AgNPs modified the mechanism of anodic dissolution and cathodic hydrogen gas evolution. These results indicated that AgNPs can be incorporated into the existing inhibitors towards minimizing corrosion rate.

Tuning of Citrate-Stabilized Laser Ablated Silver Nanoparticles for Glyphosate Detection

The recent advances in nanotechnology can help develop both fast and cost-effective detection techniques to verify if the presence of contaminants in water resources is under safe limits, increasing temporal and geographical test coverage. For a specific analyte, the detection performance of citrate-stabilized silver nanoparticles depends on their chemical affinity, as well as on the ensemble characteristic of the colloid upon its de-stabilization by a mediated aggregation process. This work presents the tuning of citrate-stabilized silver nanoparticles produced by pulsed laser ablation in liquids for glyphosate detection in water. For fixed geometry and laser pulse characteristics, the ablation time, surfactant concentration and interaction pH were studied. The resulting colloidal solutions of silver nanoparticles with glyphosate were interrogated employing optical fiber spectroscopy. Two interrogation techniques which are based on a controlled aggregation of the colloid were used: UV-Vis extinction and Surface Enhanced Raman scattering. Using a calibration curve for low concentrations, the obtained limits of detection for glyphosate in water were $6~\mu \text{M}$ (1.0 mg/L) and $7.5~\mu \text{M}$ (1.3 mg/L), respectively.

A One-Pot Synthesis and Characterization of Antibacterial Silver Nanoparticle-Cellulose Film.

Using N,N-dimethylacetamide (DMAc) as a reducing agent in the presence of PVP-K30, the stable silver nanoparticles (Ag-NPs) solution was prepared by a convenient method for the in situ reduction of silver nitrate. The cellulose–Ag-NPs composite film (CANF) was cast in the same container using lithium chloride (LiCl) giving the Ag-NPs-PVP/DMAc solution cellulose solubility as well as γ-mercaptopropyltrimethoxysilane (MPTS) to couple Ag-NPs and cellulose. The results showed that the Ag-NPs were uniformly dispersed in solution, and the solution had strong antibacterial activities. It was found that the one-pot synthesis allowed the growth of and cross-linking with cellulose processes of Ag-NPs conducted simultaneously. Approximately 61% of Ag-NPs was successfully loaded in CANF, and Ag-NPs were uniformly dispersed in the surface and internal of the composite film. The composite film exhibited good tensile properties (tensile strength could reach up to 86.4 MPa), transparency (light transmittance exceeds 70%), thermal stability, and remarkable antibacterial activities. The sterilization effect of CANF0.04 against Staphylococcus aureus and Escherichia coli exceed 99.9%. Due to low residual LiCl/DMAc and low diffusion of Ag-NPs, the composite film may have potential for applications in food packaging and bacterial barrier.

Observation of the complex spectra for the supramolecular system involving silver nanoparticles‐biaryl Schiff bases containing the nitro group

AbstractA series of biaryl Schiff bases containing the nitro groups, 4‐XArCH═NArNO2‐4′ (XBANO2‐4′) and 4‐NO2ArCH═NArY‐4′ (4‐NO2BAY), were synthesized. Also, the fish sperm DNA (fsDNA) and silver nanoparticles (AgNPs) solutions were prepared. By mixing these compounds with fsDNA or AgNPs solution and determining the ultraviolet absorption spectra of the mixture solutions, an interesting phenomenon was found: a new absorption peak λmax,lim appeared in the (XBANO2‐4′)‐AgNPs solution, which was longer than the wavelength of λmax of XBANO2‐4′ solution. The new absorption peak in the (XBANO2‐4′)‐AgNPs solution was the complex spectrum originating from the electron transfer between XBANO2‐4′ and AgNPs. Whereas this phenomenon was not observed in the (4‐NO2BAY)‐AgNPs solutions, a quantitative correlation analysis was carried out with the measured spectral data, and the results show that the wave number νmax,lim of the λmax,lim is mainly affected by the excited‐state substituent constant rather than the ground Hammett constant σ of the X group. The redshift magnitude Δνmax,WSL, namely, Δνmax,WSL = (1/λmax) − (1/λmax,lim), of the wavelength λmax,lim is related to the highest occupied molecular orbital and lowest unoccupied molecular orbital of XBANO2‐4′. The discovery of this new phenomenon is helpful to understanding the interaction between AgNPs‐organic compound supramolecular systems.

Green Synthesis of Silver Nanoparticles in the Presence of Polysaccharide: Optimization and Characterization

The process of obtaining aqueous solutions of silver nanoparticles with the use of a low-temperature nonequilibrium contact plasma and stabilizing agent—polysaccharide (sodium alginate)—has been examined. The synthesized Ag NPs were characterized by using UV-Vis spectroscopy, dynamic light scattering (DLS), scanning electron microscope (SEM), and XRD analysis. The effect of concentration of Ag+, sodium alginate, duration of processing by plasma discharge, and pH of liquid on the production of silver nanoparticles has been studied. The results demonstrated that synthesis provides the formation of silver nanoparticles for investigated concentrations of Ag+(0.3-3.0 mmol/l) and 5.0 g/l Na-Alg (pH=7–10) within 1–5 minutes. From the SEM images, the silver nanoparticles are found to be almost spherical. Powder XRD results reveal that Ag nanoparticles have a face-centered cubic crystal structure. Zeta potential of plasma-chemically obtained colloidal solutions at various concentrations of Ag+ions and stabilizing agent varies from −32.8 to −39.3 mV, indicating the moderate stability of synthesized nanoparticles.

МОРФОФУНКЦІОНАЛЬНИЙ СТАН ВІСЦЕРАЛЬНОЇ БІЛОЇ ЖИРОВОЇ ТКАНИНИ ЩУРІВ ЗА УМОВ РОЗВИТКУ ОЖИРІННЯ ТА ПРИ ВВЕДЕННІ НАНОЧАСТИНОК СРІБЛА

There was studied the morphofunctional state of the visceral white adipose tissue of rats with obesity and after introduction of silver salt solution and silver nanoparticles solution. In particular, we analyzed the morphofunctional changes in the visceral white adipose tissue of rats on a high-calorie diet, as well as the effects of the silver nitrate solution and the silver nanoparticles solution at a dose of 1 mg / 1 kg body weight and 0.5 mg / 1 kg body weight on the morphofunctional state of the visceral white adipose tissue of rats on a high-calorie diet. There were established morphofunctional changes in cells of the rats white adipose tissue on the high-calorie diet and with the introduction of silver preparations in different concentrations. The condition of the adipose tissue was evaluated on the basis of the following parameters: the relative mass of the visceral fat, the cross-sectional area of adipocytes, the relative number of adipocytes per unit area. There were compared effects of administration to animals with obesity of the silver nitrate solution at a dose of 1 mg / 1 kg body weight and 0.5 mg / 1 kg body weight, as well as a of the silver nanoparticles solution at a dose 1 mg / 1 kg body weight and 0.5 mg / 1 kg of body weight. All the above silver preparations reduced the manifestations of obesity in animals which were maintained in a high-calorie diet. In addition to the form of the silver preparation (silver nitrate solution or silver nanoparticle solution), its concentration is important. The highest efficiency was shown after the administration of the silver nanoparticles solution at a dose 0.5 mg / 1 kg of body weight. In rats of this experimental group, the studied parameters not only reached the control values, but they also exceeded them, which indicates expressed influence of the drug on the obesity manifestations. In addition, the rats showed signs of fat depletion.