Sericulture, the practice of rearing silkworms (Bombyxmori L.) for silk production, has been an integral part of global textile industries for millennia. With the advent of biotechnology, particularly advancements in molecular biology and genetics, new avenues have opened up to enhance various aspects of sericulture. This abstract clearly indicates the multifaceted applications of biotechnology in sericulture, focusing on genetic improvement of silkworms, disease management strategies, enhancement of silk production efficiency and promotion of environmental sustainability. Sericulture encompasses the entire process of silk production, from silkworm rearing to silk reeling and textile manufacturing. Historically significant and economically vital in many regions, sericulture faces contemporary challenges such as disease outbreaks, genetic limitations and environmental pressures. Biotechnology offers promising solutions by leveraging tools and techniques from molecular biology, genetics and bioprocess engineering to address these challenges and enhance the productivity, quality and sustainability of silk production.