Event Abstract Back to Event Long term implantation of silicon-based micro probe arrays Domonkos Horváth1, László Grand1, Lucia Wittner2, Balázs Dombóvári2, Sebastien Kisban3, Stanislav Herwik3, Patrick Ruther3, Hercules Neves4, György Karmos1 and István Ulbert2* 1 Péter Pázmány Catholic University, Department of Information Technology, Hungary 2 Hungarian Academy of Sciences, Institute for Psychology, Hungary 3 University of Freiburg, Department of Microsystems Engineering (IMTEK), Germany 4 Interuniversity Microelectronics Center (IMEC), Belgium Long term (1-5 weeks) neural recording ability and biocompatibility of silicon-based electrode arrays developed in the framework of the NeuroProbes project was tested in vivo in the sensory-motor cortex of rats (n = 4). One of the implanted probes had one shank, while the other comb-like probes had four 4mm long shanks, both types with nine contacts per shank. The implantation was performed under ketamine/xylazine anesthesia. One craniotomy was made for each probe with one probe per rat. The dura was not perforated prior to probe insertion. After insertion, the probe connector was placed in the midline of the implant and the entire area was sealed by dental acrylic. Local field potentials (LFP), multiple unit activity (MUA) and impedance values were measured in situ. Tissue reaction was explored with neuronal (NeuN) and glial (GFAP) staining. A complex scenario was observed in the long term behavior of the implanted silicon probes. We were able to record good quality LFP and MUA for up to 5 weeks in one rat. Corresponding impedance values were high, but the histology showed low grade tissue reaction. One device failed due to a possible structural damage reflected in the relatively intact histology pattern, extremely low impedances, low electrode noise and lack of MUA modulation. Bleeding was a major issue in the remaining two cases. If signs of bleeding occurred early after the implantation, the device was not functional due to massive cell los and gliosis. Possible later and smaller bleeding was confined to a limited extent of the cortex, and allowed to record modulated MUA from the shank distal from the bleeding. Based on our results, the most serious complication of implantation is the bleeding and the consequential tissue damage that may also cause glial shielding on the long term, as reported frequently by others. Support: NeuroProbes EU IP IST-027017. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Abstracts Citation: Horváth D, Grand L, Wittner L, Dombóvári B, Kisban S, Herwik S, Ruther P, Neves H, Karmos G and Ulbert I (2010). Long term implantation of silicon-based micro probe arrays. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00264 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 05 May 2010; Published Online: 05 May 2010. * Correspondence: István Ulbert, Hungarian Academy of Sciences, Institute for Psychology, Budapest, Hungary, ulbert@cogpsyphy.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Domonkos Horváth László Grand Lucia Wittner Balázs Dombóvári Sebastien Kisban Stanislav Herwik Patrick Ruther Hercules Neves György Karmos István Ulbert Google Domonkos Horváth László Grand Lucia Wittner Balázs Dombóvári Sebastien Kisban Stanislav Herwik Patrick Ruther Hercules Neves György Karmos István Ulbert Google Scholar Domonkos Horváth László Grand Lucia Wittner Balázs Dombóvári Sebastien Kisban Stanislav Herwik Patrick Ruther Hercules Neves György Karmos István Ulbert PubMed Domonkos Horváth László Grand Lucia Wittner Balázs Dombóvári Sebastien Kisban Stanislav Herwik Patrick Ruther Hercules Neves György Karmos István Ulbert Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.