Silica-coated Magnetic Particles Research Articles

(Deep eutectic solvent-ionic liquid)-based ferrofluid a new class of magnetic colloids for determination of tamoxifen and its metabolites in human plasma samples

In this research, a novel category of magnetic colloid was created by immobilizing an ionic liquid on the surface of silica-coated magnetic particles. This was followed by utilizing a natural hydrophobic deep eutectic solvent as a carrier. This magnetic colloid served as an adsorbent in dispersive solid-phase microextraction, combined with liquid chromatography-UV detection, for analyzing tamoxifen and its three active metabolites in plasma samples. After optimizing the effective variables in the analysis, the method underwent validation. Linearity was observed in the calibration model between 0.2 and 5.0 µg/L. The range of the detection limit was found to be 0.2–200.0 µg/L. Assessing the method's precision involved evaluating the intra- and inter-day precision at three analyte concentrations, which determined less than 7.5 % and 11.2 %, respectively. Moreover, the accuracy fell between 94.3 % and 105.1 %. The method's feasibility was evaluated using human plasma samples that ranged in concentrations of tamoxifen, N-desmethyltamoxifen, 4-hydroxytamoxifen, and endoxifen, respectively, from 28.3 to 39.7, 25.9–42.7, ≈1.3, and 3.4–7.2 μg L-1. Among the many benefits of this approach is the removal of the requirement for additional electronic accessories and dispersive solvents. It also minimizes the sample size, making it easily automatable. The method's applicability was demonstrated using human plasma samples, which provided quantification ranges for various tamoxifen metabolites. This technique has a lot of potential for studying the pharmacodynamics, therapeutic drug monitoring, andpharmaceutical kineticsof tamoxifen and its metabolites in bodily fluids, which will help with clinical research and drug analysis.

Silica-coated magnetic particles for efficient RNA extraction for SARS-CoV-2 detection

Molecular diagnostic methods to detect and quantify viral RNA in clinical samples rely on the purification of the genetic material prior to reverse transcription polymerase chain reaction (qRT-PCR). Due to the large number of samples processed in clinical laboratories, automation has become a necessity in order to increase method processivity and maximize throughput per unit of time. An attractive option for isolating viral RNA is based on the magnetic solid phase separation procedure (MSPS) using magnetic microparticles. This method offers the advantage over other alternative methods of making it possible to automate the process. In this study, we report the results of the MSPS method based on magnetic microparticles obtained by a simple synthesis process, to purify RNA from oro- and nasopharyngeal swab samples of patients suspected of COVID-19 provided by three diagnostic laboratories located in the Buenos Aires Province, Argentina. Magnetite nanoparticles of Fe3O4 (MNPs) were synthesized by the coprecipitation method and then coated with silica (SiO2) produced by hydrolysis of tetraethyl orthosilicate (TEOS). After preliminary tests on samples from the A549 human lung cell line and swabs, an extraction protocol was developed. The quantity and purity of the RNA obtained were determined by gel electrophoresis, spectrophotometry, and qRT-PCR. Tests on samples from naso- and oropharyngeal swabs were performed in order to validate the method for RNA purification in high-throughput SARS-CoV-2 diagnosis by qRT-PCR. The method was compared to the spin columns method and the automated method using commercial magnetic particles. The results show that the method developed is efficient for RNA extraction from nasal and oropharyngeal swab samples, and also comparable to other extraction methods in terms of sensitivity for SARS-CoV-2 detection. Of note, this procedure and reagents developed locally were intended to overcome the shortage of imported diagnostic supplies as the sudden spread of COVID-19 required unexpected quantities of nucleic acid isolation and diagnostic kits worldwide.

Determination of remdesivir in human plasma using (deep eutectic solvent-ionic liquid) ferrofluid microextraction combined with liquid chromatography

A microextraction technique based on ferrofluids was developed for the preconcentration and quantification of Remdesivir in human plasma samples. This method utilized a new type of magnetic colloids created by combining silica-coated magnetic particles with modified ionic liquid and natural hydrophobic deep eutectic solvent as the carrier liquid. The efficiency of the sorption and desorption steps was optimized using a chemometrics approach. Under the optimized conditions, the calibration curve exhibited linearity in the concentration range of 0.5 to 500.0 μg L−1, with a limit of detection and quantification of 0.2 and 0.5 μg L−1, respectively. The method precision was evaluated by assessing intra- and interday precision at three different analyte concentrations, yielding values of 8.9% and 16.8%, respectively. Moreover, the method accuracy fell within the range of 90.9% to 107.5%. This proposed method offers a green and environmentally friendly sample preparation technique for conducting pharmacodynamic, pharmacokinetic, and therapeutic drug monitoring studies of Remdesivir in biological fluids. Importantly, this technique eliminates the need for external energy sources or the use of dispersive solvents, providing a more efficient and sustainable approach.

An efficient extraction method for short single-stranded DNA from agarose gels in aptamer screening

With the rapid advancements in aptamer screening, the efficient extraction of short single-stranded DNA (ssDNA) from agarose gel has become a new requirement. However, the currently available products are primarily designed for double-stranded DNA (dsDNA) and exhibit limited efficacy when applied to the extraction of short ssDNA. In this study, we successfully developed a novel method based on amino-modified silica-coated magnetic particles (ASMPs) for the extraction of short ssDNA from agarose gel. The gel slices containing short ssDNA were subjected to centrifugation in a spin column/centrifugation tube assembly with silica wool, followed by the adsorption using ASMPs. Subsequently, reagents containing phosphate groups were employed to desorb ssDNA from the surface of ASMPs. Through optimization of each step, we realized remarkable efficiency in the extraction of short ssDNA. To assess the efficacy of our method, we utilized it in aptamer screening. The results demonstrated that our method outperformed three commercially available DNA gel extraction products (Q-kit, S-kit, and V-kit). The relative recovery rates of all methods were as follows: M-dNTP (100.00 %) > M-BB (63.38 %) > Q-kit (46.64 %) > S-kit (15.98 %) > V-kit (0.38 %). The results strongly suggest that the developed method holds promise for short ssDNA extraction from agarose gel.

One-Step Aerosol Synthesis of SiO2-Coated FeNi Particles by Using Swirler Connector-Assisted Spray Pyrolysis

Silica-coated soft magnetic particles are essential for some powder magnetic cores consisting of primary (coarse particles) and secondary (fine particles) soft magnetic particles in the advancement of electric devices. Herein, we report the first investigation on the direct synthesis of submicron-sized silica-coated FeNi (FeNi@SiO2) particles as the secondary particle using a connector-assisted spray pyrolysis route. Provided by computational fluid dynamics calculation in applying different connector types, i.e., T-shaped and swirler, we found that the mixing performance between FeNi and HMDSO vapor in the swirler connector played an important role in resulting heterogeneous nucleation, which is crucial for obtaining the higher coating ratio (CR) and fewer undesired nanoparticles than that of the T-shaped connector. The as-prepared submicron-sized FeNi@SiO2 particles (353 nm) with the highest CR (95.9%) demonstrated a remarkable DC bias characteristic (Isat) and eddy current loss values on a powder magnetic core, promising the practical application in manufacturing soft magnetic components.

Magnetic deep eutectic solvent-based microextraction for determination of organophosphorus flame retardants in aqueous samples: One step closer to green chemistry

A ferrofluidic hydrophobic deep eutectic solvent-based on dispersive liquid–liquid microextraction combined with gas chromatography-flame ionization detection was developed for preconcentration, separation, and quantification of organophosphorus flame retardants in aqueous samples. The magnetic deep eutectic solvent was prepared by sonicating silica-coated magnetic particles and benzyltriphenylphosphonium bromide-1-undecanol as the carrier liquid. A multivariate strategy containing central composition design and a univariate methodology were employed for optimization of the independent variables effective in the efficiency of sorption and desorption steps, respectively. Method validation study performed under optimal conditions, demonstrates the proper linear range for the analytes calibration curves with the limit of detection the range of 3.0 to 23.0 ng/L and the limit of detection lower than 50.0 ng/L. Intra- and inter-day precision measured at three concentrations for the goal analytes were lower than 5.9 and 8.5 %, respectively. The method intra-day accuracy (96.8–102.0 %) and inter-day accuracy (96.5–103.1 %) illustrate the satisfactory applicability of the proposed method for the analysis of the goal analytes in aqueous samples.

Synthesis of porous magnetic zeolite-based material and its performance on removal of Cd2+ ion and methylene blue from aqueous solution

In this study, the natural clinoptilolite zeolite was hydrothermally treated and then combined with porous silica-coated magnetic particles to prepare a porous magnetic zeolite-based material. Process parameters such as NaOH concentration, hydrothermal temperature and time, pH value, and Fe3O4/Na2SiO3 ratio were optimized. XRD, XPS, N2 adsorption-desorption technique, SEM-EDX, TEM, FTIR, etc. were employed to investigate the composition and structure of the materials. Cd2+ ion and methylene blue were adopted to evaluate the contamination removal performance of the materials. The results showed that hydrothermal treatment under an alkaline environment could transform clinoptilolite to Na–P zeolite with a low Si/Al ratio and relatively high Cd2+ adsorption capacity. Porous silica-coated magnetite loading could significantly increase the specific surface area of the material and render it magnetic. The as-prepared porous magnetic zeolite-based material exhibited much higher Cd2+ ion and methylene blue removal performance than the raw natural clinoptilolite zeolite.

High-throughput sample processing for methylation analysis in an automated, enclosed environment.

Variation in methylcytosine is perhaps the most well-studied epigenetic mechanism of gene regulation. Methods that have been developed and implemented for assessing DNA methylation require sample DNA to be extracted, purified and chemically-processed through bisulfite conversion before downstream analysis. While some automated solutions exist for each of these individual process steps, a fully integrated solution for accomplishing the entire process in a high-throughput manner has yet to be demonstrated. Thus, sample processing methods still require numerous manual steps that may reduce sample throughput and precision, while increasing the risk of contamination and human error. In this work, we present an integrated, automated solution for performing the entire sample preparation process, including DNA extraction, purification, bisulfite conversion and PCR plate preparation within in an enclosed environment. The method employs silica-coated magnetic particles that eliminate the need for a centrifuge or vacuum manifold, thereby reducing the complexity and cost of the required automation platform. Toward this end, we also compare commercial DNA extraction and bisulfite conversion kits to identify a protocol suitable for automation to significantly improve genomic and bisulfite-treated DNA yields over manufacturer protocols. Overall, this research demonstrated development of an automated protocol that offers the ability to generate high-quality, bisulfite-treated DNA samples in a high-throughput and clean environment with minimal user intervention and comparable yields to manual processing.

Enzyme Encapsulation by Facile Self-Assembly Silica-Modified Magnetic Nanoparticles for Glucose Monitoring in Urine.

Silica nanoparticles hold tremendous potential for the encapsulation of enzymes. However, aqueous alcohol solutions and catalysts are prerequisites for the production of silica nanoparticles, which are too harsh for maintaining the enzyme activity. Herein, a procedure without any organic solvents and catalysts (acidic or alkaline) is developed for the synthesis of silica-encapsulated glucose-oxidase-coated magnetic nanoparticles by a facile self-assembly route, avoiding damage of the enzyme structure in the reaction system. The encapsulated enzyme was characterized by scanning electron microscopy, transmission electron microscopy, energy-dispersive spectrometry, and a vibrating sample magnetometer. Finally, a colorimetric sensing method was developed for the detection of glucose in urine samples based on the encapsulated glucose oxidase and a hydrogen peroxide test strip. The method exhibited a good linear performance in the concentration range of 20~160 μg mL−1 and good recoveries ranging from 94.3 to 118.0%. This work proves that the self-assembly method could be employed to encapsulate glucose oxidase into silica-coated magnetic particles. The developed colorimetric sensing method shows high sensitivity, which will provide a promising tool for the detection of glucose and the monitoring of diabetes.

One-dimensional nanochains consisting of magnetic core and mesoporous aluminosilicate for use as efficient nanocatalysts

Magnetic assembly at the nanoscale level brings potential possibilities in obtaining novel delicate nanostructures with unique physical, photonic or electronic properties. Interface surfactant micelle-directed assembly strategy holds great promising in fabricating ordered mesoporous materials with multifunctionality and pore parameter tunability. Combing these, herein, one-dimensional (1D) nanochains with well-aligned silica-coated magnetic particles as core and mesoporous aluminosilicate as shell are rational fabricated for the first time through magnetic field induced interface coassembly in biliquid system followed by the incorporation of Al species via in-situ chemical modification and transformation strategy. The obtained magnetic mesoporous aluminosilicate nanochains (MMAS-NCs) possess well-defined core-shell-shell sandwich nanostructure, tunable perpendicular mesopore channels in the shell (2.7–7.6 nm), high surface area (359 m2·g−1), abundant acidic sites, and superparamagnetism with a magnetization saturation of 13.8 emu·g−1. Thanks to the unique properties, the MMAS-NCs exhibit excellent performance in acting as magnetically recyclable superior solid acid catalysts and nanostirrers with high conversion of over 96.8%, selectivity of 95.0% in the deprotection reaction of benzaldehyde dimethylacetal to benzaldehyde. Moreover, MMAS-NCs exhibit an interesting pore size effect on the catalytic activity, namely, in the pore size range of 2–8 nm, the catalysts with larger pores show significantly enhanced catalytic activity due to the balanced mass transport and density of surface active sites.

Modification of phage display technique for improved screening of high-affinity binding peptides

The phage display technique is a combinatorial technology in which random peptides are displayed on the surface of the phage; it is widely used to identify high-affinity peptides that bind to a target protein. However, this technique presents several problems due to non-specific binding of the phages and steric hindrance caused by blocking agents. To overcome these problems, we tested two modified methods and compared their screening performance with that of the conventional method. We used poly-His-tagged human epidermal growth factor receptor 2 (HER2) as a target protein and silica-coated magnetic particles (MPs) with an immobilized nickel-nitrilotriacetic acid ligand as a solid matrix. Modified method #1 (#M1) included two negative selection steps against a blocking agent (bovine serum albumin) and nude Ni-NTA MPs, after the positive selection step using immobilized HER2 on MPs in the absence of BSA. Modified method #2 (#M2) allowed the binding of phages and HER2 in solution prior to immobilizing HER2 on the MP surface. The negative selection procedure was the same between them. The binding affinity of the phages screened by #M1 and #M2 was evaluated by phage ELISA. Two phages from #M2 (#M2-4 and 5) showed the highest binding, and between them #M2-5 was selected for affinity maturation by inserting a hairpin-structured peptide between the two #M2-5 peptides. SPR study showed the HER2-binding affinity was indeed improved by ca. 2000-fold to become comparable with that of the intact antibody (trastuzumab). The modified biopanning methods combined with the dimerization maturation can be an effective way to develop antibody-mimicking peptides for affinity binding.

Extraction of highly degraded DNA from ancient bones, teeth and sediments for high-throughput sequencing.

DNA preserved in ancient bones, teeth and sediments is typically highly fragmented and present only in minute amounts. Here, we provide a highly versatile silica-based DNA extraction protocol that enables the retrieval of short (≥35 bp) or even ultrashort (≥25 bp) DNA fragments from such material with minimal carryover of substances that inhibit library preparation for high-throughput sequencing. DNA extraction can be performed with either silica spin columns, which offer the most convenient choice for manual DNA extraction, or silica-coated magnetic particles. The latter allow a substantial cost reduction as well as automation on liquid-handling systems. This protocol update replaces a now-outdated version that was published 11 years ago, before high-throughput sequencing technologies became widely available. It has been thoroughly optimized to provide the highest DNA yields from highly degraded samples, as well as fast and easy handling, requiring not more than ~15 min of hands-on time per sample.

Rapid detection of Cronobacter spp. in powdered infant formula by thermophilic helicase-dependent isothermal amplification combined with silica-coated magnetic particles separation

Cronobacter spp. (previously Enterobacter sakazakii), an opportunistic food-borne pathogen, involved in fatal infections in neonates and infants. Contaminated powdered infant formula (PIF) was considered to be the uppermost infective resource. In the present study, a thermophilic helicase-dependent isothermal amplification (tHDA) based method combined with silica-coated magnetic nanoparticles (Si-MNPs) as a DNA separator for rapid and sensitive detection of Cronobacter has been developed. The whole detection process can be completed in <3 h, with a detection limit of 100 and 101 CFU/ml Cronobacter in pure culture and artificially contaminated PIF, respectively, with a great sensitivity (94%) and specificity (100%). Hence, this protocol might be useful for screening and monitoring the contamination of Cronobacter spp. in food industry, and helpful for avoiding the economic loss by retard of feedback of the contamination of pathogens in dairy products.

Preparation of iron oxide silica particles for Zika viral RNA extraction

In this work, a robust synthetic pathway for magnetic core preparation and silica surface coating of magnetic microparticles is presented. Silica-coated magnetic particles are widely used to extract DNA and RNA from various biological samples. We present a novel route for the synthesis of iron oxide silica particles (Fe3O4@Silica) and demonstrate their performance for extracting ZIKA viral RNA from serum. The iron (II, III) oxide (Fe3O4), magnetite core is first prepared by ammonia neutralization of ferrous and ferric chloride aqueous solution under argon, followed by the addition of citrate salt to stabilize the surface of the resultant magnetic nanospheres. After this one-pot, two-step synthesis, the magnetic nanospheres are consumed during silica coating by hydrolysis of tetraethoxysilane (TEOS) under alkaline condition. The final product is a sphere-like magnetic aggregate with a size range of 1–2 micron. By simply suspending the magnetic aggregates in guanidinium chloride solution, the silica surface can be prepared for RNA binding. The RNA extraction efficiency was evaluated by extracting ZIKA viral RNA from serum followed by a PCR-based assay. The data indicate excellent recovery of target RNA and removal of PCR inhibitors. This manufacturing procedure for the silica coated microparticles provides a low-cost, effective and ready for scale-up method whose performance is equivalent to commercial alternatives such as magnetic silica surface particles for DNA and RNA sample preparations. The cost of the clinical assays could be largely decreased due to the 100 fold reduction in cost by replacing the commercially available magnetic particles with the developed material for RNA extraction.

Use of Dual-Force Aggregation as a Multiplexed, Rapid Point-of-Care Screening Method for White Blood Cell Counting from Whole Blood Samples.

Enumeration of blood cells is an integral metric for evaluating patient health and can be used to screen for a wide range of diseases and conditions. Conventional methods rely on large, expensive, and complicated instrumentation that requires trained technicians and is not amenable to point-of-care analysis. This work demonstrates the use of a multiplexed, bead-based assay for both rapid white blood cell (WBC) count screening and accurate, multiplexed WBC counts for point-of-care analysis. Blood samples were lysed and diluted before being incubated with silica-coated magnetic particles under chaotropic conditions, a rotating magnetic field, and a source of agitation. The resulting bead aggregation was imaged and correlated to a known WBC count. After establishing standard curves, the WBC count for 18 whole blood samples were determined by this method and compared to values obtained conventionally. When the optimal dilution factor for lysis of whole blood samples was established, 17 of 18 samples (94.4%) were correctly screened and categorized as having high, typical, or low WBC count, while 14 of 18 samples were within 16% of the reported clinical values. The developed system provides analysis of 13 samples in <3 min with a total analysis time of approximately 10 min (including incubation and dilution) and represents comparable throughput to conventional instrumentation, while providing point-of-care capability with reduced size (14 × 21 × 14 cm) and simplicity. This work demonstrates the potential for a multiplexed, bead-based assay to be used as a rapid, point-of-care screening method for WBC counting from whole blood samples.

A Recoverable Ruthenium Aqua Complex Supported on Silica Particles: An Efficient Epoxidation Catalyst.

The preparation and characterization of complexes with a phosphonated terpyridine (trpy) ligand (trpy-P-Et) and a bidentate pyridylpyrazole (pypz-Me) ligand, with formula [RuII (trpy-P-Et)(pypz-Me)X]n+ (2: X=Cl, n=1; 3: X=H2 O, n=2), is described, together with the anchoring of 3 on two types of supports: mesoporous silica particles (SP) and silica-coated magnetic particles (MSP). Aqua complex 3 is easily obtained by heating 2 in refluxing water and exhibits a two-electron RuIV/II redox process. It was anchored on SP and MSP supports by two different synthetic strategies, yielding the heterogeneous systems SP@3 and MSP@3, which were fully characterized by IR and UV/Vis spectroscopy, SEM, cyclic voltammetry, and differential pulse voltammetry. Catalytic olefin epoxidation was tested with molecular complex 3 and its SP@3 and MSP@3 heterogeneous counterparts, including reuse of the heterogeneous systems. The MSP@3 material can be easily recovered by a magnet, which facilitates its reusability.

Coating of Sulfonic Silica onto Magnetite from Marina Beach Iron sand, Semarang, Indonesia

The mineral iron oxide is the main component of sand iron that are abundant in nature. Mineral iron oxide not yet widely applied into more useful products. The main component of iron ore is magnetite. Magnetite can be used as a basic ingredient in the manufacture of magnetite-modified silica adsorbent sulfonate. In this research, the adsorbent made from sulfonic functionalized silica-coated magnetic particle has been successfully produced, with the magnetite was obtained from iron sand at Marina Beach, Semarang Indonesia. This adsorbent was then used as a metal ion preconcentration media. From the research that it was found that the sulfonic has been bound to the silica marked by the emergence of element S on EDX. Whilst, the evidence that silica has coated on the magnetite could be seen from the SEM images which showed the morphology of sulfonic functionalized silica-coated magnetic particles were larger than the sulfonic functionalized silica without magnetite. From the DSC results showed that the addition of magnetite on sulfonic functionalized silica did not change the heat resistance of the sulfonic functionalized silica. Based on the XRD patterns show that magnetite sulfonate silica was formed.

Facile TEMPO Immobilization onto Poly(acrylic acid)-Modified Magnetic Nanoparticles: Preparation and Property

Adding catalysts to magnetic polyvalent supports facilitating catalyst recycling and recovery seems feasible. Polymer-modified magnetic nanocomposites for organocatalyst immobilization are a plausible approach to this technology. Here, we present facile and efficient method for 2,2′,6,6′-tetramethylpiperidinyl-1-oxy (TEMPO) immobilization onto polymer-modified magnetic nanoparticles under mild reaction conditions. Poly(acrylic acid) was chosen to graft from magnetic nanoparticle through a simple inverse emulsion polymerization technique. The resulting poly(acrylic acid) magnetic nanocomposite is an ideal material to immobilize the organocatalyst 4-hydroxy-2,2′,6,6′-tetramethylpiperidinyl-1-oxy (H-TEMPO) via an esterification reaction with pendant carboxyl group on the polymer chain. Instrumental analysis confirmed that poly(acrylic acid) chain was grafted on the silica-coated magnetic particles by this simple method while maintaining their magnetic properties; elemental analysis indicated that TEMPO was efficiently immobilized onto the polymer chain. The catalysis tests under both Anelli and Minisci system showed that the nanocomposite catalyst exhibits proper selectivity and activity for the alcohol/aldehyde transformation. Recycling experiments showed that stability and reusability of the nanocomposite catalyst were satisfying.

Autonomous magnetic labelling of functional mesenchymal stem cells for improved traceability and spatial control in cell therapy applications.

Mesenchymal stem cells (MSCs) represent a valuable resource for regenerative medicine treatments for orthopaedic repair and beyond. Following developments in isolation, expansion and differentiation protocols, efforts to promote clinical translation of emerging cellular strategies now seek to improve cell delivery and targeting. This study shows efficient live MSC labelling using silica‐coated magnetic particles (MPs), which enables 3D tracking and guidance of stem cells. A procedure developed for the efficient and unassisted particle uptake was shown to support MSC viability and integrity, while surface marker expression and MSC differentiation capability were also maintained. In vitro, MSCs showed a progressive decrease in labelling over increasing culture time, which appeared to be linked to the dilution effect of cell division, rather than to particle release, and did not lead to detectable secondary particle uptake. Labelled MSC populations demonstrated magnetic responsiveness in vitro through directed migration in culture and, when seeded onto a scaffold, supporting MP‐based approaches to cell targeting. The potential of these silica‐coated MPs for MRI cell tracking of MSC populations was validated in 2D and in a cartilage repair model following cell delivery. These results highlight silica‐coated magnetic particles as a simple, safe and effective resource to enhance MSC targeting for therapeutic applications and improve patient outcomes. © 2016 The Authors Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd.

Co nanoparticle decorated magnetic core, polymeric ionic liquid shell composites for H2 production

Here, firstly iron oxide (Fe3O4) nanoparticles (NPs) were prepared and coated with silica-containing vinyl groups, and then a poly(4-vinyl pyridine) p(4-VP) polymeric shell was synthesized around the silica-coated magnetic particles by micro emulsion polymerization technique (M@p(4-VP)). The magnetic polymeric ionic liquid (PIL) particles: M@p(4-VP)+C2, M@p(4-VP)+C4 and M@p(4-VP)+C6, were then prepared by modifying M@p(4-VP) with 1,2-dibromoethane (DBE), 1,4-dibromobutane (DBB) and 1,6-dibromohexane, respectively. The magnetic PIL particles were used as template for Co metal nanoparticles (MNPs) and, M@p(4-VP)+C2@Co, M@p(4-VP)+C4@Co and M@p(4-VP)+C6@Co were created as magnetic PIL-Co composite catalyst systems. The prepared magnetic PIL-Co composite catalyst systems were used as catalysts in H2 generation from hydrolysis of NaBH4. Various parameters such as the type of PIL template, the concentration of NaOH, the number of metal ion loading reducing processes (Co amounts), the reusability, and the hydrolysis reaction temperature were investigated for their effect on H2 production. Among the prepared PIL-metal composites, M@p(4-VP)+C4 particles contained 504.6mgg−1 Co after 4 metal ion loading-reducing cycles. M@p(4-VP)+C4@Co particles exhibited excellent catalytic activity in H2 generation from the hydrolysis of NaBH4 and their hydrogen generation rate (HGR) and Turn Over Frequency (TOF) were found as 8.46±0.17 (L H2) (g of Co min)−1 and 20.79±0.10 and 10.43±0.23 (mol H2) (mol of Co min)−1, higher than most published data. Also, a very low activation energy (Ea=22.8±0.8kJmol−1) was calculated for H2 generation for the hydrolysis of NaBH4 catalyzed by M@p(4-VP)+C4@Co in comparison to similar studies.