(Deep eutectic solvent-ionic liquid)-based ferrofluid a new class of magnetic colloids for determination of tamoxifen and its metabolites in human plasma samples

Abstract

In this research, a novel category of magnetic colloid was created by immobilizing an ionic liquid on the surface of silica-coated magnetic particles. This was followed by utilizing a natural hydrophobic deep eutectic solvent as a carrier. This magnetic colloid served as an adsorbent in dispersive solid-phase microextraction, combined with liquid chromatography-UV detection, for analyzing tamoxifen and its three active metabolites in plasma samples. After optimizing the effective variables in the analysis, the method underwent validation. Linearity was observed in the calibration model between 0.2 and 5.0 µg/L. The range of the detection limit was found to be 0.2–200.0 µg/L. Assessing the method's precision involved evaluating the intra- and inter-day precision at three analyte concentrations, which determined less than 7.5 % and 11.2 %, respectively. Moreover, the accuracy fell between 94.3 % and 105.1 %. The method's feasibility was evaluated using human plasma samples that ranged in concentrations of tamoxifen, N-desmethyltamoxifen, 4-hydroxytamoxifen, and endoxifen, respectively, from 28.3 to 39.7, 25.9–42.7, ≈1.3, and 3.4–7.2 μg L-1. Among the many benefits of this approach is the removal of the requirement for additional electronic accessories and dispersive solvents. It also minimizes the sample size, making it easily automatable. The method's applicability was demonstrated using human plasma samples, which provided quantification ranges for various tamoxifen metabolites. This technique has a lot of potential for studying the pharmacodynamics, therapeutic drug monitoring, andpharmaceutical kineticsof tamoxifen and its metabolites in bodily fluids, which will help with clinical research and drug analysis.