Significant Effect Of Time Research Articles

Heart Rate Variability in Concussed College Athletes: Follow-Up Study and Biological Sex Differences.

Finding reliable biomarkers to assess concussions could play a pivotal role in diagnosis, monitoring, and predicting associated risks. The present study aimed to explore the use of heart rate variability (HRV) in the follow-up of concussions among college athletes and to investigate the relationships between biological sex, symptomatology, and HRV values at baseline and after a concussion. Correlations between measures were also analyzed. A total of 169 (55 females) athletes aged 16 to 22 years old completed baseline testing, and 30 (8 females) concussion cases were followed. Baseline assessment (T1) included psychosocial and psychological questionnaires, symptoms report, and four minutes of HRV recording. In the event of a concussion, athletes underwent re-testing within 72 h (T2) and before returning to play (T3). Baseline findings revealed that girls had higher %VLF while sitting than boys, and a small negligible correlation was identified between %HF and total symptoms score as well as %HF and affective sx. Post-concussion analyses demonstrated a significant effect of time × position × biological sex for %HF, where girls exhibited higher %HF at T3. These findings suggest disruptions in HRV following a concussion and underscore biological sex as an important factor in the analysis of HRV variation in concussion recovery trajectory.

The Landing Biomechanics in Youth Female Handball Players Does Not Change When Applying a Specific Model of Game and Weekly Training Workload

This study aimed to explore the effects of competitive match play and subsequent training during typical competitive microcycle on landing biomechanics in female youth handball players. A group of 11 elite female youth players (age: 14.3 ± 0.6 years; stature: 165.9 ± 8.1 cm; body mass: 58.4 ± 10.6 kg; maturity offset: 0.4 ± 0.8 years) were tested prior to a competitive match, immediately after the match, 48 h after the match, 96 h after the match, and before the next match. The players performed two analyzed trials of a single leg (preferred) counter movement jump. The “Landing Error Scoring System” (LESS) was used to analyze the participants’ landing biomechanics. Results: There was no significant effect of a competitive handball match on LESS (Z = 0.28; p = 0.78). No statistically significant difference in LESS was found between the first and the last measurement (Z = 1.01; p = 0.31). No significant main effect of time was found for landing biomechanics in the observed eight-day period (χ2 = 4.02; p = 0.40). The results of the study indicate that a model of weekly loading during in-season, including competitive match play, does not decrease lower limb biomechanics during landing and does not contribute to an increased risk of anterior cruciate ligament injury in female youth handball players during a competitive microcycle.

Effects of Physical Exercise on Executive Functions among College Students in China: Exploring the Influence of Exercise Intensity and Duration.

This study investigates the effects of exercise intensity and duration on executive functions among college students in China. Participants in this study were Chinese college students divided into four groups based on exercise duration and intensity. Each group engaged in physical exercise twice a week for six weeks. Group 1 performed low-intensity exercises for 10 min per session; Group 2 performed low-intensity exercises for 20 min per session; Group 3 performed high-intensity exercises for 10 min per session; and Group 4 performed high-intensity exercise for 20 min per session. Executive functions were assessed in all subjects before the experiment (time 1), after one exercise session (time 2), at the end of the exercise program (time 3), and six weeks after the exercise program (time 4). A mixed ANOVA with a 2 (exercise intensity: low/high) × 2 (duration: short/long) × 4 (time of measurement: time 1/time 2/time 3/time 4) design was employed, with exercise intensity and exercise duration as independent variables and executive functions as the dependent variable. Mixed ANOVA showed that the results revealed a significant main effect of measurement time on working memory accuracy (p < 0.001) and reaction time (p < 0.001); inhibition control accuracy (p < 0.001) and reaction time (p < 0.001); cognitive flexibility accuracy (p < 0.001) and reaction time (p < 0.001). A single session of high-intensity exercise significantly improved executive functions in college students. Both low-intensity and high-intensity exercise were effective in enhancing executive functions, with high-intensity exercise demonstrating better maintenance of the effect. Both exercise intensity and exercise duration were found to enhance executive functions in college students, with exercise intensity showing greater effectiveness than exercise duration.

Time-Varying Determinants of Graft Failure in Pediatric Kidney Transplantation in Europe.

Little is known about the time-varying determinants of kidney graft failure in children. We performed a retrospective study of primary pediatric kidney transplant recipients (younger than 18 years) from the Eurotransplant registry (1990-2020). Piece-wise exponential additive mixed models were applied to analyze time-varying recipient, donor, and transplant risk factors. Primary outcome was death-censored graft failure. We report on 4528 kidney transplantations, of which 68% with deceased and 32% with living donor. One thousand six hundred and thirty-eight recipients experienced graft failure, and 168 died with a functioning graft. Between 2011 and 2020, the 5-year graft failure risk was 10% for deceased donor and 4% for living donor kidney transplant recipients. Risk of graft failure decreased five-fold from 1990 to 2020. The association between living donor transplantation and the lower risk of graft failure was strongest in the first month post-transplant (adjusted hazard ratio, 0.58; 95% confidence interval, 0.46 to 0.73) and remained statistically significant until 12 years post-transplant. Risk factors for graft failure in the first 2 years were deceased donor younger than 12 years or older than 46 years, potentially recurrent kidney disease, and panel-reactive antibody >0%. Other determinants of graft failure included dialysis before transplantation (until 5 years post-transplant), human leukocyte antigen mismatch 2-4 (0-15 years post-transplant), human leukocyte antigen mismatch 5-6 (2-12 years post-transplant), and hemodialysis (8-14 years post-transplant). Recipients older than 11 years at transplantation had a higher risk of graft failure 1-8 years post-transplant compared with other age groups, whereas young recipients had a lower risk throughout follow-up. Analysis of the combined effect of post-transplant time and recipient age showed a higher rate of graft failure during the first 5 years post-transplant in adolescents compared with young transplant recipients. In contrast to deceased donor younger than 12 years, deceased donor older than 46 years was consistently associated with a higher graft failure risk. We report a long-term inverse association between living donor kidney transplantation and the risk of graft failure. The determinants of graft failure varied with time. There was a significant cumulative effect of adolescence and time post-transplant. The ideal donor age window was dependent on time post-transplant.

Discharge transitional care programme for older adults after hip fracture surgery: a quasi-experimental study.

Hip fractures require extended periods of recovery and rehabilitation, subjecting older adults to discontinuous care. Discharge transition is a critical point of heightened vulnerability for older adults. This study aimed to evaluate the effectiveness of a transitional care programme on the physical functions and quality of life (QOL) of older adults after hip fracture surgery. Seventy-five older adults were assessed from pre-discharge to 6 weeks after hip surgery, and their physical functions, including walking status and activities of daily living, were measured. The QOL was measured using the European Quality of life-5 Dimensions-5 Levels (EQ 5D 5L). There was a significant strong effect of time (B = 10.565; 95% CI = 2.584-18.547; p = 0.009) on the EuroQol Visual Analog Scale (EQ-VAS) for the experimental group. However, there were no significant effects of time on physical functions and EQ-5D-5L scores. The discharge transitional care programme improved the EQ-VAS of older adults following hip fracture surgery 6 weeks post-surgery. However, there were no significant differences in physical functions and EQ-5D between the groups.

Warming and salinization effects on the deep-water rose shrimp, Parapenaeus longirostris, distribution along the NW Mediterranean Sea: Implications for bottom trawl fisheries

The deep-water rose shrimp is a main resource for the GSA 6 bottom trawling fleet. In the last decade, landings have increased without a clear understanding of the causes. This study aims to analyze this trend, potentially related to changes in environmental conditions. Results showed an increase in the species' landings, which spread northwards along the GSA 6. GAM models detected a significant effect of location, time, and depth on the distribution of the deep-water rose shrimp, as did for temperature and salinity. Similar values between landings and LPUE were found throughout, suggesting no effects of fishing effort in time. ANOVA tests showed a significant increase of sea bottom temperature and salinity in time, which were correlated with increasing LPUE values. Then, the trend seems to be related to environmental changes rather than changes in fishing effort. Further research is needed to implement management plans that ensure the resource sustainability.

Effect of Personalized Blue-Enriched White Light Intervention on Rest-Activity and Light Exposure Rhythms in Mild and Moderate Alzheimer's Disease.

We aimed to examine the effectiveness of personalized light intervention using a blue-enriched light-emitting-diodes device on rest-activity rhythm (RAR) and light exposure rhythm (LER) in patients with mild and moderate Alzheimer's disease (AD). AD patients with poor sleep quality and/or insomnia symptoms were assigned into either an experimental group (EG) or control group (CG) in a single-blind design. Personalized light intervention was given at 9-10 h after individual dim light melatonin onset, lasting for 1 h every day for two weeks in the EG (77.36±5.79 years, n=14) and CG (78.10±7.98 years, n=10). Each patient of CG wore blue-attenuating sunglasses during the intervention. Actigraphy recording at home for 5 days was done at baseline (T0), immediate postintervention (T1), and at four weeks after intervention (T2). The variables of RAR and LER were derived using nonparametric analysis. We found a significant time effect on the intradaily variability (IV) of RAR at T2 with respect to T0 (p=0.039), indicating reduced IV of RAR at four weeks after personalized light intervention regardless of blue-enriched light intervention. There was a time effect on the IV of LER at T1 with respect to T0 (p=0.052), indicating a reduced tendency in the IV of LER immediately after intervention. Our personalized light intervention, regardless of blue-enriched light source, could be useful in alleviating fragmentation of RAR and LER in AD patients.

Do Adult Naïve Listeners Perceive Differences in Speech Before and After Therapy for Cleft Palate Speech Disorders? A Reliability Study of Perceptual Speech Ratings.

This study compared the interrater reliability of adult naïve listeners' perceptual assessments of different speech variables in children with a cleft palate with or without a cleft lip (CP ± L). In addition, the study investigated whether the listeners were able to perceive differences in these speech variables before and after speech therapy for cleft palate speech disorders. Thirty-four speech samples of 14 children with a CP ± L (14 samples collected immediately before 10 hr of speech intervention, 14 samples collected immediately after speech intervention, and six randomly selected samples that were duplicated to assess intrarater reliability) were perceptually assessed by 26 adult naïve listeners. The listening panel consisted of nine men and 17 women (age range: 18-51 years). The speech variables included speech understandability, speech acceptability, hypernasality, hyponasality, nasal airflow, and articulation, which were assessed on a visual analog scale. Furthermore, the need for speech therapy was assessed. Good to very good interrater reliability was observed for the naïve listeners' ratings of all speech variables. A significant time effect was found for the pre- and postevolution of the speech variables "speech understandability," "speech acceptability," "nasal airflow," and "articulation." This time effect indicates an improvement of these variables postintervention. According to the naïve listeners, children were less in need of additional speech therapy after the 10-hr intervention period compared to assessments before this intervention period. Adult naïve listeners perceptually identified an improvement in different speech variables after 10 hr of cleft palate speech therapy. These findings confirm previous assessments of expert speech-language pathologists and suggest that speech improvements after cleft palate speech therapy can also be perceived by communication partners outside the therapy room. Perceptual ratings of naïve listeners can, thus, be used to add life-situation significance to the assessments of experts. Future research could include both expert raters and caregivers or relatives of children with a CP ± L in listening panels, as previous knowledge on craniofacial anomalies may lead to different results.

Longitudinal change in sleep, functional, and behavioural characteristics in a cohort of children with Down syndrome.

This paper describes the longitudinal change in sleep, functional, and behavioural characteristics in a cohort of children with Down syndrome, including the effect of sleep interventions in a subset. A prospective longitudinal cohort study was undertaken in children with Down syndrome aged 3-16 years comparing (1) children referred to a tertiary sleep medicine clinic who received sleep hygiene advice and an additional sleep treatment (DSref_I) with (2) children attending the same clinic who only received sleep hygiene advice (DSref_N) and (3) children recruited from the community who, were not receiving any treatment (DScomm). Data collected included demographic and medical history information, Child Sleep Habits Questionnaire-Abbreviated (CSHQ-A), Life-Habits Questionnaire (Life-H) and Child Behaviour Checklist (CBCL) at baseline and then 6-monthly for a total of 18 months. Any sleep interventions during this time were recorded. A total of 57 children were included (DSref_I, n = 16; DSref_N, n = 25; DScomm, n = 16). At recruitment, the median CSHQ-A total score was high (>41) in all three subgroups, but highest in the DSref_I subgroup (median [interquartile range] Dsref_I score 58 [53-66] versus DSref_N score 49 [43-53], p = 0.019). Although improved, 80% of participants in the DSref_I subgroup still had a CSHQ-A total score >41 at the last assessment point. The median total Life-H and total CBCL scores were not significantly different between groups at baseline and there was no significant time, group, or interaction effect seen through the study. Over an 18-month period, sleep problems were seen to persist in children with Down syndrome. Treatment resulted in only modest improvements in sleep.

Energy and Macronutrient Intakes in Young Athletes: A Systematic Review and Meta-analysis.

The aim of this study was to conduct a systematic review and meta-analysis of differences in energy and macronutrient intakes between young athletes and non-athletes, considering age, gender and sport characteristics. The study included original research articles that compared energy and macronutrient intakes of 8 to 18-year-old athletes to non-athletes. Mean difference (MD) meta-analyses were performed to quantify energy and macronutrient intake differences between athletes and non-athletes. Eighteen observational studies were included. Results revealed that the energy and carbohydrate consumption of athletes was higher than that of non-athletes (MD=4.65kcal/kg/d, p<0.01 and MD=1.65% of total energy intake, p<0.01, respectively). Subgroup analyses revealed a significant effect of total training time on the observed mean differences between athletes and non-athletes. As practice time increased, the differences between athletes and non-athletes increased for carbohydrate and decreased for protein. Sport type analysis revealed a higher protein intake by mixed sport athletes compared to endurance and power sports. Analyses also indicated an age effect: the older the athletes, the smaller the differences between athletes and non-athletes for energy intake. However, the methods used to match groups and estimate dietary intakes forced us to moderate the results. More rigorous research methods are needed to define the dietary intakes of athletes and non-athletes.

More than fat – Proteins in dairy and plant milks contribute to the reduction of oral burn from capsaicin

The effectiveness of cow’s milk in reducing capsaicin-induced burn is well-known, and typically attributed to capsaicin’s lipophilicity. Still, controlled laboratory studies show full-fat milk is not more efficacious than fat-free milk, suggesting other components may contribute to milk’s effectiveness. Recent research has shown milk proteins like micellar casein and whey reduce capsaicin burn in the absence of fat. This paper reports two experiments in moderate capsaicin users that investigate the efficacy of dairy and plant milks varying in fat and protein content. In Experiment One, participants were exposed to 5-ppm capsaicin solutions before rinsing with conventional full-fat milk, ultra-filtered full-fat milk, almond milk, soy milk, and pea protein-enriched flax milk. In Experiment Two, they rinsed with fat-free, conventional full-fat, and ultra-filtered full-fat milk, and three soy milks of varying protein content. Participants made burn ratings on a general Labelled Magnitude Scale (gLMS) once every 10 s for 2-min (Experiment One) or continuously for 2-min (Experiment Two). Experiment One showed significant main effects of time and sample, with planned comparisons revealing differences for water versus conventional and ultra-filtered full-fat milk. In Experiment Two, a main effect of time and a time-by-sample interaction were observed, with samples in both blocks (soy milk, dairy milk) significantly outperforming water. Some evidence of a concentration-dependent effect was observed for protein, with both experiments suggesting ultra-filtered high protein full-fat milk was most efficacious. These findings suggest proteins play a role in mitigating capsaicin burn, although further research is needed to quantify the relative contributions of fat and protein.

Abstract 13139: Preliminary Efficacy of a Cognitive Restorative Nature Intervention to Improve Attention, Self-Care, and Health-Related Quality of Life in Heart Failure

Background: Cognitive dysfunction is prevalent among patients with heart failure (HF) and decreases self-care behaviors and health-related quality of life (HRQL). Goal: The purpose was to test preliminary efficacy of a newly developed virtual reality-based cognitive restorative nature intervention (Nature-VR based on Attention Restoration Theory) on improving attention, HF self-care, and HRQL. Methods: In this 2-group randomized controlled trial, 73 patients with Stage C HF were enrolled. The Nature-VR intervention group (n=37) viewed 3-dimensional nature pictures delivered by a virtual reality headset for 10 minutes/day, 5 days/week for 4 weeks. The attention control group (n=36) viewed 3-dimensional urban pictures (Urban-VR) delivered by a virtual reality headset at the same frequency of Nature-VR. Outcomes were: attention measured by Oral Trail Making A and Digit Span Forward; HF self-care measured by Self-care of Heart Failure Index; and HRQL measured by Minnesota Living with Heart Failure Questionnaire. Outcomes were assessed at baseline, 4, 8, 26, and 52 weeks by telephone interviews due to COVID-19 pandemic. Linear mixed models with intention-to-treat analysis were used to test effects of group, time, and group-by-time interactions. Results: Of 73 patients, 41 were women. Self-reported race was 1 Asian, 12 African American, and 60 White. Mean age was 66 years. NYHA class was I/II=33, III=30, and 10 were missing. Sixty-eight patients (93%) completed the interventions; 56 (79% of 68) had ≥ 80% adherence; 51 (70%) completed the study. In mixed models, no significant group-by-time interactions were found in attention, HF self-care, and HRQL. HF self-care (maintenance, management, symptom perception, and confidence) significantly improved over time (p &lt; .001) in both groups. There was significant improvement on symptom perception (p= .004, better in Nature-VR). HRQL showed significant group (p = .041, worse in Nature-VR) and time effects (p = .047, both groups improved). Conclusions: Although preliminary efficacy of Nature-VR compared with Urban-VR was not supported, HF self-care and HRQL improved over 52 weeks. Future testing is indicated of multimodal interventions that include Nature-VR to improve improving self-care and HRQL.

Efficacy of spironolactone as adjunctive therapy to sodium valproate in bipolar-I disorder: A double-blind, randomized, placebo-controlled clinical trial.

Treatment of mood and cognitive symptoms of patients with bipolar disorder is associated with many complications and is generally not associated with therapeutic satisfaction. In this clinical trial, we evaluated the effectiveness of spironolactone in controlling mood and cognitive symptoms, sleep quality, appetite, and body mass index in patients with bipolar disorder in manic episodes. Sixty inpatients with bipolar disorder in manic episodes were treated with spironolactone/placebo in an 8-week randomized, double-blind, placebo-controlled clinical trial. They were evaluated using the Young Mania Rating Scale (YMRS), mini-mental state examination (MMSE), Pittsburgh sleep quality index, Simplified Nutritional Appetite Questionnaire, and body mass index in weeks 1, 4, and 8. For cognitive impairment (MMSE), there were significant interaction effects of group and time at week 8 (B=-1.60, SE=0.69, t=-2.33, p=.021) such that individuals in the spironolactone group experienced more improvement in their cognitive performance. For manic symptoms (YMRS), there were no significant interaction effects of group and time at week 8 (B=-2.53, SE=1.46, t=-1.73, p=.085). Considering the promising findings in this clinical trial, further study of spironolactone as adjunctive therapy in bipolar disorder in manic episodes with larger sample sizes, multicenter settings, and longer follow-ups are recommended.

432 Fluctuations in Equine Cutaneous Ph and Trans-Epidermal Water Loss Associated with Anatomical Location, Time, and Weather

Abstract Although skin health is of critical importance for all horse owners, understanding of cutaneous pH and trans-epidermal water loss (TEWL) and the normal ranges of these variables is lacking. Cutaneous pH and TEWL measures provide insight into the barrier function of the epidermis and have the potential to determine the resident dermal microbiota. However, these measures have been recorded to fluctuate due to a variety of factors such as environment and anatomical locations in other species. Accordingly, the objectives of this study were to identify the impacts of anatomical location, weather, and time of day on cutaneous pH and TEWL in equines. This study utilized nine (n = 9) stock type mares, group-housed in a pasture setting at Southern Illinois University Equine Center. Researchers measured cutaneous pH and TEWL three times (6:00 AM, 12:00 PM, 6:00 PM) daily across five anatomical locations (nose, whither, heart-girth, mid-back, and base of tail) for five consecutive days. All statistical analysis was conducted using SAS On Demand for Academics (P &amp;lt; 0.05). Analysis of changes in variables associated with time, anatomical location, and day were conducted utilizing a PROC GLM three-way repeated measures ANOVA utilizing a Greenhouse-Geiser correction. Cutaneous pH results displayed significant main effects of day (P = 0.0052) and time (P = 0.0073) as well as a significant interaction of day and location (P = 0.0004). Mean cutaneous pH fluctuated daily, however, certain anatomical locations fluctuated more than others each day. Additionally, pH was highest in the morning (mean of 7.28) and lowest in the evening (mean 7.011). However, the mean overall pH for the study is 7.14 which is congruent with other work. TEWL measurements had significant main effects of day (P &amp;lt; 0.0001), time (P &amp;lt; 0.0001), and location (P = 0.0231), as well as a significant interaction of day, time, and location (P &amp;lt; 0.0001). TEWL measurements fluctuated with day, likely a result of weather changes. Mean TEWL measurements were less in the morning (mean 24.68) and greatest in the afternoons (mean 38.52). Overall mean TEWL measurement was 30.25. The results of this work indicate fluctuations in dermal barrier variables are associated with day, anatomical location, and time of day. These findings may have implications for applications of dermal treatments for horses as well as catalog the natural fluctuations which may affect future research.

PSIII-4 Effect of Photobiomodulation Treatment on Atp Concentrations in Bovine Oocytes During Maturation

Abstract Oocyte cytoplasmic maturation is critical in determining the success of subsequent embryo development. It has been proposed that light in the red-to-near infrared optical region (~600–1000 nm) enhances cellular metabolic activity through activation of the mitochondrial respiratory chain, increasing intracellular ATP concentrations, and has the potential to modify the oocyte metabolome. Previous research indicated a greater cleavage rate and similar blastocyst rates for bovine oocytes treated with photobiomodulation at hours 16 and 20 from the start of maturation compared with control. For the present study, two separate experiments were conducted to determine ATP concentration in oocytes during maturation after photobiomodulation treatment. A standardized in vitro maturation (IVM) protocol and commercial media (IVF Bioscience) were used. Briefly, bovine ovaries were obtained at a local slaughterhouse, cumulus oocyte complexes (COCs) were collected by aspiration of small (2 to 6 mm) follicles. For experiment 1, compact COCs were cultured in in vitro maturation media (n = 30/well) and treated with no light (control); light (660-665nm) for 10 minutes at hour 16 from start of maturation (L-16) and light for 10 minutes at hour 20 from the start of maturation (L-20). At hour 24 of in vitro maturation, COCs were vortexed to remove cumulus, stained with BioTracker ATP-Red Live Cell Dye (Sigma-Aldrich SCT045), imaged with Cytation-1 (Agilent Technologies Inc.) in 6 replicates. For experiment 2, COCs were cultured in in vitro maturation media (n = 40/well) and treated with no light (control) and light (660-665nm) for 10 minutes at hour 16 from start of maturation (L-16). At hours 18, 20, 22 and 24 of in vitro maturation, COCs were vortexed to remove cumulus, stained with BioTracker ATP-Red Live Cell Dye, and imaged with Cytation-1 in 4 replicates. For experiment 1, one-way ANOVA and Student’s T-Test were performed (JMP software 16.0); in experiment 2, each well was considered as an experimental unit in a model of repeated measures, main effects of time and treatment, blocked by replicate (Mixed procedure, SAS Institute Inc.). In experiment 1, there was a significant (P &amp;lt; 0.05) effect of treatment; ATP concentrations were greater in L-16 than control, and L-20 was intermediate. ATP concentrations were 19,579.3a ± 596.5, 21,652.0b ± 602.7 and 19,710.9ab ± 570.6 relative fluorescence units (RFU) for control, L-16, and L-20 respectively. In experiment 2, there was a significant (P &amp;lt; 0.05) effect of treatment and no significant effect of time. Overall ATP concentrations were 25,668 ± 894.6 and 28,332 ± 894.6 RFU for control and L-16 respectively. In conclusion, photobiomodulation treatment with LED 660-665nm for 10 minutes at hour 16 from start of oocyte maturation increases intracellular ATP concentrations from hour 18 to 24 of maturation.

347 Effects of Inoculation Period on the Nutritive Value of Pleurotus Ostreatus Treated Corn Stover

Abstract The objective of the study was to optimize and improve the feeding value of corn stover. Despite the documented ability of Pleurotus ostreatus to selectively delignify crop residues, it is not widely used for ruminant fiber digestion partly due to extended pretreatment time. Optimization of crop residues is influenced by enzymatic activities of fiber degrading enzymes and dependent on the stage of P. ostreatus growth. Thus, optimizing the inoculation period for lignin removal by P. ostreatus is important to improve and increase success on lignocellulose feed resources utilization. Therefore, we hypothesized a difference in nutritive value for different inoculation periods for P. ostreatus spent substrates. Two ruminally cannulated dairy cows were used in an in vitro study arranged in a 3 x 5 factorial design with 4 replicates to evaluate the effects of inoculation period [0 (control), 2, 4, 6 and 8 wk] on P. ostreatus spent substrates at 6, 24 and 48 h of fermentation on chemical composition, dry matter disappearance (DMd) and volatile fatty acid (VFA) production. Significant (P &amp;lt; 0.001) non-linear treatment effect across the inoculation period was observed in the treated samples (Table 1). An increase of 44.4 to 59.1% and 20.6 to 78.6% was noted for the crude protein and ash contents, respectively. Also, significant (P &amp;lt; 0.001) incubation time effect across the inoculation period resulted in a wide variation (22.6 - 755%) in the microbial mass with greater values for 2- and 4-wk treatments. Neutral detergent fiber and hemicellulose were significantly (P &amp;lt; 0.001) reduced with a range between 7.29 to 18.7% and 26.6 to 40.8%, respectively (Table 3). Inconsistent positive and negative outcomes across the periods in acid detergent fiber, lignin and cellulose resulted in significant (P &amp;lt; 0.001) 116 and 121% increment in non-fiber carbohydrates for 2 and 4-wk treatments, respectively. Consequently, both DMd and total VFA were significantly (P &amp;lt; 0.001) greater, ranging between 238 to 242% and 13.9 to 15.1% respectively for these treatments (Table 2). Additionally, the rumen fermentation pathway favored production of propionate with significant (P &amp;lt; 0.001) treatment and time effect that resulted in 7.46 and 8.30% increment in propionate production after 2 and 4 wk, respectively. Consequently, significant (P &amp;lt; 0.001) reduction was observed in acetate with 4.44 and 3.58%, and 11.7 and 11.1% in A:P ratio for 2 and 4 wk, respectively. High butyrate production was observed for 2 wk in comparison with 4 wk resulting in 18.3 and 9.86% increment, respectively, when compared with control (Table 4). The study showed that 2 wk of inoculation is sufficient to provide bio-transformed cow-calf feed resource from P. ostreatus treated corn stover, which is contrary to 30 days (4 wk) previously recommended.

345 Bioconversion Treatment of Corn Stover Can Create Cheap Feed Resource for Limited Resource Farmers

Abstract The study aimed to improve the nutritive value of corn stover to provide a viable and sustainable source of cheap feed resource for ruminant production since feed is the single largest cost in any livestock enterprise. Corn stover is the largest non-food biomass available in the United States. It could be a low-cost feed, but high lignocellulose content limits both digestibility and nutritive value. Some fungi, especially white rot fungi (WRF), are known to selectively degrade fiber lignin and secrete bioactive compounds that could benefit digestibility and feed value. Pleurotus ostreatus is a mushroom-forming WRF. We tested corn stover fermented with P. ostreatus for improvement in nutrients and digestibility (Table 1). Two ruminally cannulated dairy cows were used in an in vitro study to test the effect of P. ostreatus use in solid state fermentation on the chemical composition in corn stover. The experiment was 2 x 3 factorial design with 4 replicates sampled at 6, 24 and 48 h on treated and untreated (control) corn stover. The dry matter disappearance (DMd) and volatile fatty acid (VFA) production (Table 2). Treatments were 1) untreated corn stover as control, and 2) corn stover treated with P. ostreatus. Crude protein (CP) and ash were significantly (P &amp;lt; 0.001) impacted with 52.9 and 31.2% increment in CP and ash respectively for treated samples. We noted an increase (P &amp;lt; 0.001) of 106 to 681% in microbial yield in treated vs untreated samples which demonstrated a greater potential for increased microbial protein synthesis. Acid detergent fiber, neutral detergent fiber and hemicellulose were significantly reduced (P &amp;lt; 0.001) by 6.71, 18.2 and 37.8%, respectively for the treated samples. Non-fiber carbohydrate was significantly (P &amp;lt; 0.001) impacted with a 118% increment and both DMd and total VFA were significantly (P &amp;lt; 0.001) greater, ranging between 40.9 to 240% and 5.85 to 11.2%, respectively (Table 3). Additionally, the rumen fermentation pathway favored the production of propionate with a significant (P &amp;lt; 0.001) treatment and time effect that resulted in 9.37 - 14.0% increment in propionate production for the treated samples. Consequently, a significant (P &amp;lt; 0.001) effect was observed in acetate production with 1.76 - 4.01% reduction, which resulted in 7.09 - 11.42% reduction in A:P ratio for the treated samples (Table 4). The study showed that bioconversion can improve the feeding value of corn stover to provide an economical novel feed resource, and that corn stover spent mushroom substrate could potentially substitute conventional feeds to support animal requirements. Although actual cow performance remains to be determined experimentally via feeding studies, we are convinced that bioconversion of agricultural waste from crop farming could be a functional system approach to alleviate the feed cost challenges faced by socially disadvantaged and limited-resource farmers.

Positive imagery in depressive suicidal patients: A randomized controlled trial of the effect of viewing loved ones’ photos on mood states and suicidal ideation

According to research, it has been suggested that individuals who are affected by depression could potentially engage in the creation and experience emotional advantages relating to positive events directed towards the past or future, with the condition that they are provided with suitable mental imagery techniques. The main aim of this study was to assess the impact of utilizing positive imagery, specifically through the utilization of photographs featuring loved ones, on mood states and suicidal ideation among individuals diagnosed with depression and exhibiting suicidal tendencies. This randomized, double-blind, controlled crossover trial was conducted among 78 hospitalized depressive patients at three psychiatric services between April and August 2019. The patients participated in four individual picture-viewing sessions on four consecutive days. The four categories of pictures were included: loved ones, neutral faces of strangers, natural landscapes, and optical illusions. Directly prior to and immediately following the observation of the visual stimuli (photographs), the Brunel Mood Scale (BRUMS) and the Beck Scale for Suicide Ideation (BSSI) were completed by the patients. Repeated measures ANOVAs conducted in this study revealed a significant main effect of time on ratings of tension, depression, fatigue, vigor, calmness, and happiness (P values < 0.001). Additionally, statistically significant interactions were identified between picture category and time in relation to the variables of tension, depression, fatigue, vigor, calmness, and happiness (P values < 0.001). The analysis did not reveal a significant main effect of time on ratings of anger, confusion, and suicidal ideation (P values > 0.05). Likewise, the interaction between picture category and time did not yield significant results for the variables of anger, confusion, and suicidal ideation (P values > 0.05). The positive imagery procedure using the presentation of loved ones’ photos showed beneficial effects on the mood states of depressed patients. The findings of this study suggest that incorporating a greater emphasis on positive imagery within the context of clinical depression may offer potential advantages. This highlights the potential for novel opportunities in the treatment of depression. Trial registrationThe study has been registered in the Iranian Registry of Clinical Trials (registration number: IRCT20180808040744N1; first registration date: December 22, 2018; website: https://en.irct.ir/trial/33186).

Hypothalamic-pituitary-adrenocortical response in alcohol dependent patients during baclofen treatment and association with clinical outcome: preliminary results.

Baclofen has been shown to reduce alcohol consumption in some individuals with alcohol use disorder. This preliminary study aimed to evaluate i) the effect of baclofen versus placebo on hypothalamic-pituitary-adrenocortical activity (HPA-axis), as measured by cortisol, and ii) the relationship between clinical outcomes such as alcohol consumption on a randomised controlled trial of baclofen (BAC) versus placebo (PL) (Kirsten C. Morley et al., 2018; K. C. Morley, Leung, Baillie, & Haber, 2013). We hypothesised that baclofen will reduce HPA axis activity following a mild stressor in patients with alcohol dependence. Plasma cortisol levels were taken from N = 25 alcohol dependent patients at two time points, approximately 60 (pre MRI scan: PreCortisol) and 180 minutes (post MRI scan: PostCortisol) following administration of PL, BAC 10 mg or BAC 25 mg. Participants were followed-up for the remaining 10 weeks as part of the trial for clinical outcome (percentage days abstinent). Mixed models revealed a significant main effect of medication on cortisol levels (F = 3.88, p = 0.037), no significant effect of time (F = 0.04, p = 0.84) and a significant time x medication (F = 3.54, p = 0.049). Linear regression (F = 6.98, p = 0.01, R2=0.66) revealed that abstinence at follow-up, weighted by gender, was predicted by blunted cortisol response (ß= -0.48 p = 0.023), in addition to medication (ß= 0.73 p = 0.003). In conclusion, our preliminary data suggest that baclofen moderates HPA axis activity, as measured by blood cortisol, and that these alterations may play a role in long term treatment response.

5 Meta-Analysis of Cognitive Functioning Following Non-Severe COVID-19 Infection

Objective:To effectively diagnose and treat cognitive post-COVID-19 symptoms, it is important to understand objective cognitive difficulties across the range of acute COVID-19 severity. The aim of this meta-analysis is to describe objective neuropsychological test performance in individuals with non-severe (mild/moderate) COVID-19 cases in the post-acute stage of infection (&gt;28 days after initial infection).Participants and Methods:This meta-analysis was pre-registered with Prospero (CRD42021293124) and utilized the PRISMA reporting guidelines, with screening conducted by at least two independent reviewers for all aspects of the screening and data extraction process. Inclusion criteria were established before the article search and were as follows: (1) Studies using adult participants with a probable or formal and documented diagnosis of COVID-19 in the post-acute stage of infection; (2) Studies comparing cognitive functioning using objective neuropsychological tests in one or more COVID-19 groups and a comparison group, or one group designs using tests with normative data; (3) Asymptomatic, mild, or moderate cases of COVID-19. Twenty-seven articles (n=18,202) with three types of study designs and three articles with additional longitudinal data met our full criteria.Results:Individuals with non-severe initial COVID-19 infection demonstrated worse cognitive performance compared to healthy comparison participants (d=-0.412 [95% CI, -0.718, -0.176)], p=0.001). We used metaregression to examine the relationship between both average age of the sample and time since initial COVID-19 infection (as covariates in two independent models) and effect size in studies with comparison groups. There was no significant effect for age (b=-0.027 [95% CI (0.091, 0.038)], p=0.42). There was a significant effect for time since diagnosis, with a small improvement in cognitive performance for every day following initial acute COVID-19 infection (b=0.011 [95% CI (0.0039, 0.0174)], p=0.002). However, those with mild (non-hospitalized) initial COVID-19 infections performed better than did those who were hospitalized for initial COVID-19 infections (d=0.253 [95% CI (0.372, 0.134)], p&lt;0.001). For studies that used normative data comparisons, there was a small, non-significant effect compared to normative data (d=-0.165 [95% CI (-0.333, 0.003)], p=0.055).Conclusions:Individuals who have recovered from non-severe cases of COVID-19 may be at risk for cognitive decline or impairment and may benefit from cognitive health interventions.