Significant Reduction Research Articles

Effects of fat unsaturation degree on whipping performance and foam stability of fat‐reduced aerated emulsions

SummaryAerated emulsions formulated with vegetable oils have been widely consumed with health demands of tea‐based drinks and coffee. In comparison with its traditional counterparts consisting of 30%–40% of fats, that is, whipping creams, the aerated emulsions containing less than 20% vegetable oils are classified as low fat‐cream‐like products and popular with consumers preferring fat‐reduced foods. However, unstable whipping capabilities are the typical quality defects of these low fat‐emulsions, which is highly related with partial coalescence of fat globules. In the present study, effects of unsaturation degree and fatty acid species of fat matrix on whipping capabilities of the aerated emulsions were evaluated by analysing crystal networks, partial coalescence behaviours, whipping performance, and foam structure and stabilities. Increase of fat unsaturation from 10% to 30% improved fat crystal structure and partial coalescence significantly. In particular, 10%–15% of medium chain‐fatty acids present in the emulsions with 30% unsaturated fats tended to crystallise into well‐packed thicker crystals, and to exhibit the highest partial coalescence rate with nearly 60% of solid fat content at whipping temperature. Both the fat species and unsaturation contributed to forming smooth appearance and desirable foam networks after whipping. In contrast, further increase in unsaturation resulted in significant reduction of adsorbed proteins, contributing to excessive aggregation of fat globules. Therefore, it presented negative effects on partial coalescence as well as foam firmness and stabilities. The results figured out characteristics of fat matrix for the manufacture of low fat‐aerated creams with improved whipping capabilities.

Surgical and medical outcomes in robotic compared to laparoscopic colectomy global prospective cohort from the American college of surgeons national surgical quality improvement program.

Evidence regarding the outcomes benefits of robotic approach, when compared to a laparoscopic approach, in colectomy remain limited. This study aimed to analyze the value of robotic approach compared to laparoscopic approach in minimally invasive colectomy. Cohort study of the National Surgical Quality Improvement Program (NSQIP). This study included data from the NSQIP from 1/2016 to 12/2021. Adult patients undergoing minimally invasive (laparoscopic or robotic) colorectal surgery. Robotic versus laparoscopic colectomy. Risk ratios for the incidence of medical and surgical morbidity and overall mortality. Compared to laparoscopic, robotic colectomy was associated with a significant decrease in postoperative morbidity [RR 0.84 (95%CI 0.72-0.96), P < 0.001], a significant reduction in postoperative mortality [RR 0.83 (95%CI 0.79-0.90), P 0.010)], and in post operative ileus [RR: 0.80 (95%CI 0.75-0.84), P < 0.001]. Yet, robotic approach was associated with a significant increase in total operative time despite a significant decrease in total length of stay. No benefit was observed regarding anastomotic leak. Observational nature of the study cannot exclude residual bias. In this prospective cohort from the NSQIP, robotic colectomy was associated with a significant reduction in postoperative ileus, unplanned conversion to open surgery, morbidity, and overall mortality when compared to laparoscopic colectomy.

A Study to Assess the Effectiveness of Therapeutic Touch Massage on Reduction Level of Pain &amp; Anxiety among Stroke Patients Admitted in Shri Vinoba Bhave Civil Hospital Silvassa, Dadra and Nagar Haveli

BACKGROUND: Stroke is the leading cause of disability worldwide and the second leading cause of death. The Global Stroke Factsheet released in 2022 reveals that lifetime risk of developing a stroke has increased by 50% over the last 17 years and now 1 in 4 people is estimated to have a stroke in their lifetime. OBJECTIVE: This study aims to assess the effect of therapeutic touch massage on reduction level of pain and anxiety among the stroke patients admitted in Shri Vinoba Bhave Civil Hospital, Silvassa. METHODS: A quantitative quasi experimental research design was adopted and samples were allotted in experimental group (n=25) and control group (n=25) by purposive sampling technique. Sociodemographic and clinical variables data was collected using the self-administered questionaries and pain, anxiety and spasticity assessed by abbey pain scale, Hamilton anxiety scale and modified Ashworth scale. Respectively, The therapeutic touch massage was performed under the supervision of the investigator for 30 minutes daily for 6 days. RESULT: The results indicate a significant reduction in pain level after therapeutic touch massage (pretest mean = 9.88, posttest mean = 6.80;t= 6.959,df=48,p&lt;0.05) &amp; reduction in anxiety level non-significant change after therapeutic touch massage ( pretest = 21.04 , posttest = 18.28 ; df = 48, p&gt;0.05).Various Sociodemographic variables showed significant association with pretest pain level in experimental group &amp; no significant association were found with pretest anxiety level in experimental group. A positive correlation was found between pretest pain &amp; anxiety in experimental group &amp; control group. CONCLUSION: Therapeutic touch massage is useful in relieves pain and anxiety, aids in relaxation, relieves the effects of pain and improves sleep. KEYWORDS: Therapeutic touch massage; pain; anxiety; stroke patients; complementary therapy.

Development of TiO2/Nb2O5 Films and Evaluation of Corrosion Prevention in AISI 304

The combination of TiO2 and Nb2O5 in thin films has proven promising for preventing the corrosion of AISI 304. The thin films are developed using sol–gel and dip‐coating techniques, followed by heat treatment. Morphological and X‐ray diffraction analyses indicate a predominant anatase phase in TiO2, and isomorphic substitution of up to 20% Nb occurs in the thin‐film composition. Phase segregation above 30% is observed. Electrochemical tests are conducted in a 2 mol L−1 HCl solution. Scanning electron microscopy reveals satisfactory thin film deposition on AISI 304 but shows the presence of regions with discontinuities. Polarization curves show a corrosion current of 128.72 μA for pure AISI 304, an average corrosion current of 0.34 μA for thin films, and an average of 6.44 μA for films with discontinuities. This significant reduction in current demonstrates the efficiency of the corrosion protection of the film at the studied concentrations because of the high corrosion inhibition percentages. Electrochemical impedance spectroscopy results suggest a high charge transfer resistance, indicating a lower corrosion rate in xTiO2 the yNb2O5 films compared to uncoated AISI 304.

Tunable anomalous resistance and large magnetoresistance in HfTe5 by atom doping

The Dirac layered material HfTe5 renews significant interest due to its exotic band structure, leading to abundant transport properties, such as the anomaly resistance peak and its large magnetoresistance. Here, we prepared single crystals HfTe5 and Cr-doped CrxHf1−xTe5 and carried out their electrical transport measurements to explore the underlying physical origin of the anomaly resistance behavior and the large magnetoresistance. An anomalous resistance peak was observed in both intrinsic HfTe5 and the Cr-doped ones. Specifically, the peak temperature in the doped ones experiences an obvious shift from 52 to 34 K as the doping concentration x increases from 0 to 0.15, as well as the magnitude of the peak resistance is significantly enhanced. Furthermore, the magnetoresistance of CrxHf1−xTe5 is reduced by more than one order of magnitude compared with the intrinsic one. The significant reduction in magnetoresistance after Cr doping is attributed to the breaking of the balance between electron and hole carriers, which is confirmed by Kohler's plots. Meanwhile, in the sample where the magnetoresistance was minimized, we observed Shubnikov–de Haas oscillations. These observations illustrate that the large magnetoresistance is primarily contributed by the compensation of electrons and holes rather than the high mobility. Our findings provide valuable insight into how to engineer HfTe5 to achieve large magnetoresistance and its further applications in magnetic sensors and spintronics.

Effect of Gracilaria vermiculophylla Macroalga on Non-Alcoholic Fatty Liver Disease in Obese Rats.

Marine algae are valuable sources of bioactive compounds that have the potential to be used in the management of various pathologies. Despite the increasing prevalence of NAFLD, the absence of an approved effective pharmacological treatment with demonstrable effectiveness persists. In this context, the aim of the present study is to assess the effect of Gracilaria vermiculophylla red seaweed dietary supplementation on hepatic lipid accumulation, as well as on oxidative stress, inflammation and fibrosis- related markers on obese fa/fa Zucker rats fed with a standard diet, supplemented or not with 2.5% or 5% dehydrated Gracilaria vermiculophylla. After a six-week supplementation with the macroalga, no significant reduction in hepatic total lipid content or hepatic triglyceride content was observed. However, both doses were able to diminish hepatic NEFA concentration by reducing de novo lipogenesis and increasing mitochondrial biogenesis. Moreover, supplementation with the dose of 2.5% improved some oxidative stress and inflammation-related markers. Supplementation with the dose of 5% did not exert these clear beneficial effects. Thus, this study demonstrates that while Gracilaria vermiculophylla may not mitigate hepatic steatosis, it could exert protective effects on the liver by reducing NEFA content and enhancing oxidative stress and inflammation parameters.

Enhancing saltiness perception in bone broth: the additive effect of oil and optimization of sodium-reduction formula for consumer acceptability.

Excessive NaCl intake in liquid and semi-solid food (e.g. soup, hot pot base, sauce) poses a high risk to human health, and reducing NaCl intake is a major concern for global health. Using the generalized Labeled Magnitude Scale (gLMS) method, the study verified the possibility of sodium reduction through oil addition. The compromised acceptance threshold (CAT) and hedonic rejection threshold (HRT) were determined. The gLMS results showed that the saltiness intensity of samples containing 0.36% NaCl and 2.29% sunflower seed oil was significantly higher than that of samples containing only 0.36% NaCl (P < 0.05). CAT and HRT results indicated that by adding 3.59% sunflower oil, the NaCl content could be reduced to a minimum of 0.14% without causing sensory rejection in bone broth samples. The quantitative descriptive analysis method was used to determine the effects of NaCl and oil concentrations on the sensory attributes of bone broth samples. Furthermore, it was used to analyze the consumer acceptability drivers in combination with the hedonic scale to optimize the formulation of reduced-salt bone broth products. Notably, sample E (0.36% NaCl, 2.29% fat) not only had a significant salt reduction effect with a 20% decrease in NaCl, but also had improved overall acceptability. This study provides theoretical guidance for designing salt-reduction cuisine within the catering and food industries, including bone broth and hot pot bases. © 2024 Society of Chemical Industry.

Wastewater Phytopurification Monitoring with Congolese Planted Filters, Republic of Congo

The present study aimed to evaluate the purifying power of two Congolese plant filters or emerging macrophytes EP-F (Echinochloa pyramidalis (Lam) Hutch) and CA-F (Cyperus alternifolius L.) on wastewater coming from the Total market, the largest national market of Brazzaville in Congo. To do this, we set up an experimental device composed of gravel, fine sand and two plants. These plants were cultivated in an experimental pilot consisting of 8 tanks of 42,000 cm3 of wastewater at treat each one, in addition to 2 tanks used as controls. The purifying performance study of the system after 90 days of treatment showed a significant reduction in physical, organic and mineral pollution. Using the planted filter EP-F (Echinochloa pyramidalis (Lam)Hutch), we obtain reduction rates of almost 100% for turbidity and for suspended matter (SM), 75.42% for COD, 76.5% for NH4+ ions, 99.5% for NO3- ions and 99.73% for PO43- ions. In the case of the CA-F (Cyperus alternifolius L) planted filter, we obtain reduction rates of almost 100% for turbidity and SM, 80.94% for COD, 93% for ions. NH4+, 89.11% for NO3- ions and 98.93% for PO43- ions. Furthermore, we also noted an increase in organic and mineral pollutants concentrations in the two plants compared to the raw plants before wastewater passage. This study showed that Echinochloa pyramidalis (Lam) Hutch and Cyperus alternifolius L. are effective macrophyte filters to eliminate physical, organic and mineral pollution.

Effectiveness of betahistine as an add‐on therapy to epley maneuver for benign paroxysmal positional vertigo: A systematic review and meta‐analysis

AbstractObjectiveEpley maneuver is the most effective treatment for benign paroxysmal positional vertigo (BPPV). Betahistine has been shown to be effective in treating BPPV. This systematic review aims to compare the effectiveness of Epley maneuver with betahistine to Epley maneuver alone in reducing residual dizziness in BPPV.Data SourcesMedline, Embase, and CENTRAL.MethodsWe included randomized controlled trials (RCTs) that compared Epley maneuver with betahistine to Epley maneuver alone for treating BPPV. We evaluated the effectiveness of this intervention using Dizziness Handicap Inventory (DHI), Visual Analog Scale (VAS) for vertigo, and provocation maneuvers. We used the standardized mean difference (SMD) for continuous outcomes and the odds ratio (OR) for the dichotomous outcomes.ResultsA total of eight RCTs that enrolled 516 participants were deemed eligible. Administration of betahistine with Epley maneuver showed no clinically significant difference over Epley maneuver alone in DHI score (SMD: −0.11, 95% confidence interval [CI], −0.57 to 0.34, p = 0.63, I2 = 79%), VAS scores (SMD: −0.57, 95% CI, −1.57 to 0.43, p = 0.26, I2 = 89%), or on provocation maneuvers (OR: 1.84, 95% CI, 0.92 to 3.68, p = 0.08, I2 = 0%) after 1 week of betahistine administration. However, participants who received betahistine combined with Epley maneuver showed a statistically significant reduction in VAS scores after 4 weeks of betahistine administration (SMD: −0.89, 95% CI, −1.30 to −0.49, p &lt; 0.0001, I2 = 33%).ConclusionCombining betahistine with Epley maneuver could improve the outcomes of BPPV in the long term. However, clinical trials with longer follow‐up periods are needed to unravel its efficacy.

Effectiveness of Yoga in Reducing Fatigue Among Cancer Patients at HCG Cancer Centre, Gulbarga

Cancer, a global health challenge, demands holistic care, especially for enduring issues like fatigue. Yoga emerges as a key intervention, notably in breast cancer cases. This study, conducted at HCG Cancer Centre, Gulbarga, with 50 participants, evaluates yoga's effectiveness in reducing fatigue using a randomized control design. Demographics and fatigue levels, measured by the BFI scale, are gathered. Results spotlight a significant reduction in fatigue post-yoga intervention for the experimental group, in contrast to the control. This research pioneers’ evidence-based nursing practices, showcasing yoga's potential in alleviating cancer-related fatigue for enhanced patient well-being. In simpler terms, the data strongly indicates a substantial decrease in fatigue levels for the experimental group post-yoga intervention. The mean pre-test fatigue score of 3.6 significantly dropped to 2.04 in the post-test, with a highly significant p-value of 2.8250. This underscores yoga's effectiveness in reducing fatigue among experimental subjects. In the control group, minimal changes in fatigue levels were noted without yoga intervention, as evident from the post-test data. Statistical analysis, with a p-value of 0.432, confirms nonsignificant changes in fatigue scores for the control group. This emphasizes the potential positive impact of yoga in mitigating fatigue levels, distinct from the minimal changes observed in the control group. The findings stress the need for critical interpretation of fatigue score changes and highlight yoga's positive role in addressing cancer-related fatigue, evident through significant outcomes in the experimental group. Keywords: Cancer, Yoga, Fatigue

Symptomatic rib fracture nonunion: a systematic review of the literature.

Rib fractures commonly occur in trauma patients with varying presentations. Though the literature in recent years has moved toward favoring more early intervention of acute rib fractures, little has been reported on the matter of surgical fixation for symptomatic rib fracture nonunions. We performed a review of PubMed and Cochrane databases for articles published since 2000. Inclusion criteria were studies with greater than six months of follow-up, while case studies were excluded. A thorough analysis was performed on patient outcomes, complications reported, operative techniques utilized, and fixation systems used, among other parameters reported by the articles. One hundred and thirty-nine studies resulted from our review, and a total of nine studies met our inclusion criteria with a combined total of 182 patients who underwent open reduction and internal fixation for symptomatic rib fracture nonunions. All studies reported a significant reduction of pain with increased satisfaction in the majority of patients. There were a total of 71 postoperative complications, the most common of which included surgical site infections, hardware failure, and hematoma. The most serious complications were insulting injury to the lung parenchyma or pleura; however, these were extremely rare based off the current literature. The use of bone grafting was common with eight of the nine studies mentioning the benefits of grafting. Surgical stabilization of rib fracture nonunions appears to be an appropriate treatment alternative, and various techniques and approaches may be used with similar success. Further studies with higher level of evidence are recommended on the subject.

Amorphous carbon nitride (C3N4)

This detailed investigation employs an ab initio approach to explore the atomic structure and electronic properties of an amorphous carbon nitride (C3N4) model. The model, designed with an exact 3:4 ratio, is based on an amorphous boron nitride configuration. The study reveals crucial insights into the mean coordination number for C and N atoms within the amorphous structure. With values of 2.95 for C atoms and 2.21 for N atoms, these coordination numbers closely resemble those observed in graphite-like crystals. The local structure of the amorphous network exhibits similarities to the triazine-based graphitic C3N4 crystal and is notably devoid of homopolar bonds. The estimated band gap for the amorphous C3N4 model is 1.2 eV, representing a significant reduction compared to the crystal structure, which exhibits a band gap of about 2.93 eV as determined through GGA+U calculations.

A nanoscale perspective of the coexistence of multidimensional defects in the AgCuTe system

AgCuTe, a p-type medium-temperature thermoelectric material, exhibits great potential as a candidate for converting waste heat into electricity owing to its low thermal conductivity and high thermoelectric performance. However, an unstable phase structure of the material system has hindered the direct observation of various point defects contacting with high performance, despite their predicted possibilities solely based on first-principles calculations. Here, we present the novel observation of Te interstitial atoms together with other point defects, planar defects, and nanoscale atomic aggregation for the first time in the Zn-doped and Te non-stoichiometric AgCuTe system, employing a nanoscale perspective. Through our investigation, we disclose the remarkable coexistence of multidimensional defects, thereby resolving the previously conflicting opinions regarding point defects in this system. The effective control of these defects is crucial for achieving high thermoelectric performance. The ZnCu point defects regulate carrier concentration and Seebeck coefficient by influencing phase proportion and carrier effective mass. While maintaining the material's inherently large power factor (PF), thermal conductivity is effectively reduced through grain refinement and multiscale defect scattering. As a result, the dimensionless thermoelectric figure of merit (ZT) of approximately ∼ 1.16 is achieved at 773 K. By altering Te content, a coexistence of diverse defects is introduced, leading to intricate microstructures and phase structures. Consequently, a significant reduction in thermal conductivity is observed. The coexistence of multidimensional defects, as well as their impact on microstructure and thermoelectric transport properties, holds significant implications for the performance control of functional materials.

The Design of Low Power Op-amp for Biomedical Application

This work presents a low-power operational amplifier (op-amp) circuit design which optimized and tailored specifically for biomedical circuit application in low power environment. The proposed op-amp design incorporates the folded cascode differential amplifier technique with a low voltage supply 1.2 V, utilizing current biasing, current mirrors, and adopted low-power circuit topologies. AC and DC analysis are carried out to analyse the performance of the proposed design. Extensive simulations executed using industrial standard EDA tool have validated the circuit design, and demonstrated a gain of 70.94 dB, UGBW of 4.90 MHz, GM of 52.70 dB, PM of 82.02 degrees, PSRR of 82.77 dB, CMRR of 100.78 dB, and total power dissipation of only 28.07 mW. Low power consumption is essential for biomedical circuit applications, and the result shows a significant power reduction without compromising other performance parameters. The proposed op-amp circuit design is suitable to be implemented in low-power and high-performance biomedical devices.

Ostwald ripening for designing time-dependent crystal hydrogels.

Ostwald ripening (OR), a classic solution theory describing molecular transfer from metastable crystal to stable one, is applied to design time-dependent crystal hydrogels that can automatically change their mechanical properties. Using a system made from crosslinked polyacrylamide (PAM) and sodium acetate (NaAc), we demonstrate that metastable fibrous crystal networks of NaAc preferably form in PAM hydrogels via a polymer-involving mismatch nucleation. These fibrous crystals would undergo OR and evolve into isolated bulk crystals, leading to a significant reduction in material rigidity (179 folds) and interfacial adhesion (20 folds). This transformation can be applied to program time-dependent self-recovery in shape and self-delamination. Since OR is a ubiquitous, robust feature of various crystals, the approach reported here represents a new direction for designing advanced transient soft materials.

An Intercomparison of Snow Mass Budget over Arctic Sea Ice Simulated by CMIP6 Models

Abstract The Arctic has experienced rapid changes in recent decades. For the first time, we intercompare five snow mass budget processes over Arctic sea ice simulated by 22 models from the Coupled Model Intercomparison Project phase 6 (CMIP6) using new diagnostics that have not been available for previous CMIPs. Our analysis suggests that snowfall accumulation (melt) is the dominant process contributing to nearly 100% (70.4% ± 10.1%) of the annual snow growth (loss). Snow mass change through sea ice dynamics, snow–ice conversion, and sublimation contribute 10.9% ± 4.9%, 9.7% ± 5.9%, and 9.0% ± 7.7% to the total snow mass loss. The seasonal cycle of various snow processes simulated by most of the CMIP6 models generally follows similar variations. There is reduced snowfall accumulation, melt, and sea ice dynamics during 1993–2014. However, substantial temporal and spatial discrepancies are noteworthy between the CMIP6 models. There is a large spread of snowfall accumulation and snowmelt in summer and fall, snow–ice conversion from autumn to spring, sublimation in late spring and summer, and snow mass change due to sea ice dynamics from winter to midspring. About half the models show decreasing trends of snowfall accumulation during 1993–2014, with no trends in others. Divergent trends in snow–ice conversion and sublimation occur in the Greenland and Barents Seas. The discrepancies are attributed equally to internal variability and model structural differences. Future projections that remove the identified outlier models suggest a significant reduction in snowfall accumulation, snowmelt, and snow mass change due to sea ice dynamics in the Arctic Ocean from 2015 to 2099. Snow–ice conversion and sublimation are also projected to be reduced but with less confidence.

Ostwald ripening for designing time‐dependent crystal hydrogels

AbstractOstwald ripening (OR), a classic solution theory describing molecular transfer from metastable crystal to stable one, is applied to design time‐dependent crystal hydrogels that can automatically change their mechanical properties. Using a system made from crosslinked polyacrylamide (PAM) and sodium acetate (NaAc), we demonstrate that metastable fibrous crystal networks of NaAc preferably form in PAM hydrogels via a polymer‐involving mismatch nucleation. These fibrous crystals would undergo OR and evolve into isolated bulk crystals, leading to a significant reduction in material rigidity (179 folds) and interfacial adhesion (20 folds). This transformation can be applied to program time‐dependent self‐recovery in shape and self‐delamination. Since OR is a ubiquitous, robust feature of various crystals, the approach reported here represents a new direction for designing advanced transient soft materials.

Safety and efficacy of early corticosteroid withdrawal in liver transplant recipients: A randomized controlled trial.

Prolonged use of steroids after liver transplantation (LT) significantly increases the risk of diabetes or cardiovascular disease, which can adversely affect patient outcomes. Our study evaluated the effectiveness and safety of early steroid withdrawal within the first year following LT. This study was conducted as an open-label, multicenter, randomized controlled trial. Liver transplant recipients were randomly assigned to one of the following two groups: Group 1, in which steroids were withdrawn two weeks posttransplantation, and Group 2, in which steroids were withdrawn three months posttransplantation. This study included participants aged 20 to 70 years who were scheduled to undergo a single-organ liver transplant from a living or deceased donor at one of the four participating centers. Between November 2012 and August 2020, 115 patients were selected and randomized into two groups, with 60 in Group 1 and 55 in Group 2. The incidence of new-onset diabetes after transplantation (NODAT) was notably higher in Group 1 (32.4%) than in Group 2 (10.0%) in the per-protocol set. Although biopsy-proven acute rejection, graft failure, and mortality did not occur, the median tacrolimus trough level/dose/weight in Group 1 exceeded that in Group 2. No significant differences in safety parameters, such as infection and recurrence of hepatocellular carcinoma, were observed between the two groups. The present study did not find a significant reduction in the incidence of NODAT in the early steroid withdrawal group. Our study suggests that steroid withdrawal three months posttransplantation is a standard and safe immunosuppressive strategy for LT patients.

Influence of propiconazole and metconazole formulations on Bacillus subtilis vegetative cell growth and disease control of fruit crops.

Biological control agent (BCA) Bacillus subtilis formulated as Theia® is registered for control of fungal and bacterial diseases of fruit crops. Combinations of Theia® and strategic concentrations of two demethylation inhibitor (DMI) fungicides were investigated to explore potential synergisms. Bacteria were cultured in nutrient broth and combined with technical grades and two formulations of propiconazole (EC and WP) and metconazole (EC and WDG) at 0, 10, 50, 100, and 150 µg/ml active ingredient. After co-cultivation, optical density (OD600) and colony forming units (CFU/ml) were evaluated. In contrast to EC formulations, the WP or WDG formulations at 10 or 50 µg/ml of both DMIs did not affect vegetative cell growth. The mixture of Theia® and each formulated DMI at 50 µg/ml active ingredient resulted in a significant reduction of Monilinia fructicola lesion development on apple, Colletotrichum siamense lesion development on cherry, and Botrytis cinerea lesion development on cherry. The combination of Theia® with EC formulations showed weaker disease reduction due to antagonism. Only Theia® + non-EC formulated propiconazole and metconazole significantly reduced brown rot disease incidence of apple compared to the respective solo treatments and anthracnose disease incidence of cherry compared to the untreated control. Our results indicated that at least some DMI fungicides possess bactericidal effects depending on the formulation and concentration. The combination of Theia® with a lower than label rate concentration (50 µg/ml) of the DMI fungicides propiconazole and metconazole showed potential for synergistic effects especially when non-EC formulations were used.

Sirt5-mediated lysine desuccinylation regulates oxidative stress adaptation in Magnaporthe oryzae during host intracellular infection.

Plant pathogenic fungi elaborate numerous detoxification strategies to suppress host reactive oxygen species (ROS), but their coordination is not well-understood. Here, we show that Sirt5-mediated protein desuccinylation in Magnaporthe oryzae is central to host ROS detoxification. SIRT5 encodes a desuccinylase important for virulence via adaptation to host oxidative stress. Quantitative proteomics analysis identified a large number of succinylated proteins targeted by Sirt5, most of which were mitochondrial proteins involved in oxidative phosphorylation, TCA cycle, and fatty acid oxidation. Deletion of SIRT5 resulted in hypersuccinylation of detoxification-related enzymes, and significant reduction in NADPH : NADP+ and GSH : GSSG ratios, disrupting redox balance and impeding invasive growth. Sirt5 desuccinylated thioredoxin Trx2 and glutathione peroxidase Hyr1 to activate their enzyme activity, likely by affecting proper folding. Altogether, this work demonstrates the importance of Sirt5-mediated desuccinylation in controlling fungal process required for detoxifying host ROS during M. oryzae infection.