Significant Increase In Mitotic Index Research Articles

Modulation of cytotoxic effects of wastewater on barely seedlings by Aloe vera extract

Aim: This study was conducted to assess the mitigation effect of A. vera leaf ethanolic and water extracts on plant growth, MDA and protein content, and mitotic index of barley seedlings irrigated with sewage water. Methodology: The experiment was conducted in a green house in Al-Ahsa, Saudi Arabia, in plastic bags containing air-dried loam soil and compost (1:1). Wastewater samples were collected from Al-Ahsa Sewage Treatment Plant/Al-Ahsa/Saudi Arabia. Seeds of barley were exposed to the following treatments for three weeks: 100% treated wastewater, treated wastewater mixed with: 0.05 g ml-1 A. vera alcoholic extract (Alc Ext) or 0.05 g ml-1 A. vera water extract (Wat Ext), 0.15 g ml-1 Alc Ext or 0.15 g ml-1 Wat Ext. Similar test doses were prepared for untreated wastewater. A separate set of seeds was prepared using distilled water as a control. Germination rate, fresh and dry weight, protein content, MDA content and mitotic index were determined. Results: Untreated wastewater caused significant reduction in fresh weight while both treated and untreated wastewater showed insignificant decrease in dry weight of barley. No significant change in MDA contents were observed in all treatments, except for addition of 0.15 A. vera water extract to untreated wastewater. Protein content showed an increase in both treated wastewater (insignificant) and untreated wastewater (significant). Addition of A. vera extracts increased accumulation of protein in both shoot and root. A. vera alcoholic extract promoted significant increase in both root and shoot protein of treated wastewater, but only shoot protein of untreated wastewater. A significant reduction in mitotic index was induced by wastewater, while addition of A. vera extracts stimulated significant increase in mitotic index. Interpretation: A. vera possess reactive oxygen species scavenging ability and enzyme conservation activity in addition to stimulatory effects on cell proliferation and cell-cycle process that add a modulation credit on protein content and mitotic index of barley.

Effect of transdermal immunomodulation on liver regeneration

Introduction. The use of immunomodulators to regulate reparative processes in affected organs and tissues remains a pressing issue. Of greatest interest is liver regeneration after extended hepatic resection (EHR) in donors in right lobe living related donor liver transplantation. We propose a transdermal therapeutic system (TTS) with an immunomodulator to enhance the natural process of liver tissue regeneration. Objective: to study the effect of transdermal administration of immunomodulator sodium aminodihydrophthalazinedione on early recovery processes in the liver after EHR in in vivo experiments.Materials and methods. Sodium aminodihydrophthalazinedione was used as an active substance in TTS in the form of powder for preparation of intramuscular injection solution (Galavit®, SELVIM LLC). An experimental EHR model was performed on 22 male Wistar rats weighing 350–380 g. After HER, all animals were divided into two groups. Group 1 (n = 10) consisted of untreated animals. In group 2 (n = 12), TTS was applied immediately after liver resection. The experiment lasted for 48 hours; the TTS was changed once after 24 hours from the beginning of application.Results. In either group, there was no significant difference in the weight of liver remnant gain and in biochemical blood parameters at 48 hours after EHR. Assessment of the mitotic index (MI) of hepatocytes 48 hours after EHR revealed a significant increase in MI in both groups in comparison with the baseline (before liver resection) equal to 0.14 ± 0.07‰. The MI in group 1 and group 2 animals was 12.70 ± 4.9‰ and 17.43 ± 4.90‰, respectively (p ≤ 0.05).Conclusion. Studies on the regenerative activity of sodium aminodihydrophthalazinedione TTS on an experimental EHR model in rats showed that this drug form had a pronounced stimulating effect on the mitotic activity of liver cells.

The Effect of Artemisia Dracunculus L. on Mitotic Index in Bone Marrow and Spleen Cells of Mice: In Vivo Study

Artemisia dracunculus L. (tarragon) is a rich source of herbal remedies with antioxidant and anti-inflammatoryproperties. In the present study, the proliferation of bone marrow and spleen cells of mice was evaluated afterextraction of Artemisia dracunculus with 80% of ethanol. Two doses of the extract (500mg/kg body weightand 1000mg/kg body weight) were given to the mice for seven successive days. On day eight, mice weresacrificed and cells from bone marrow and spleen were collected; mitotic index was calculated and resultswere compared with that of methotrexate at a dose 20mg/kg body weight ( positive control ) and distilledwater ( negative control). The results showed that the dose 500mg/kg body weight of Artemisia dracunculusextract caused significant increase in mitotic index in both bone marrow and spleen cells of mice whencompared with negative control. While, the dose 1000mg/kg body weight of Artemisia dracunculus extractcaused significant decrease in mitotic index in both bone marrow and spleen cells of mice when comparedwith negative control.

Genetic Study on the Effect of the Antidiabetic Drug ( Sitagliptin) on DNA and Chromosomes of Human Lymphocyte Culture

AbstractBackground: Diabetes mellitus is a worsening worldwide health problem. It constitutes a major global health concern. Sitagliptin selectively inhibits dipeptidyl peptidase-4 (DPP-4) to manage type 2 diabetes mellitus (T2DM). It regulates the blood glucose level without risk of hypoglycemia or increase in body weight. In our study we investigated the effects of Sitagliptin on DNA and chromosomes in cultured human lymphocytes. Aim: To assess the genotoxic and cytotoxic effects of different concentrations of Sitagliptin on cultured human lymphocytes.Material and methods: Cultures were divided into 6 groups: control, positive control (Cisplatin) at concentration of 10 μg/mL and 4 different concentrations of Sitagliptin (125,250,500,1000 μg/mL). Sitagliptin genotoxicity and cytotoxicity were determined by using chromosomal aberrations (CAs), mitotic index (MI), comet assay and nucleic acids electrophoresis.Results: There was high significant increase in total chromosomal aberrations (TCAs) at 500, 1000 μg/mL of Sitagliptin compared to control. Other studied concentrations of Sitagliptin exhibited an increase in TCAs without significant relation. Compared to control, there was a significant increase in mitotic index at 125 μg/mL of Sitagliptin but non-significant increase at 250 μg/mL of Sitagliptin. However, at 500, 1000 μg/mL of Sitagliptin, there was a significant decrease in MI. Regarding comet assay, there was significant and high significant increase in total DNA damage at 500,1000 μg/mL of Sitagliptin respectively. Nucleic acids electrophoresis not digested with RNase showed that optical density value of RNA was maximum at 125 μg/mL then gradually decreased till reach the minimum level at 1000 μg/mL of Sitagliptin indicating its toxicity. Genomic DNA fragmentation results indicated that Sitagliptin caused a slight damage of DNA in the form of necrosis in a concentration dependent manner. Conclusion: Sitagliptin induces significant genotoxic and cytotoxic effects on the cultured human lymphocytes at concentrations of (500, 1000 μg/mL).

Biomonitoring of urban wastewaters treated by an integrated system combining microalgae and constructed wetlands

The objectives of the present study were to apply different, toxicological assays for monitoring the toxicity of treated and untreated urban effluents produced at a university campus. The research was conducted at the wastewater treatment plant of the University of Santa Cruz do Sul, (UNISC), from october 2018 to april 2019. An integrated system with, anaerobic reactor (AR), microalgae (MA) and constructed wetlands (CWs) was, proposed for detoxification of the wastewaters produced at the university campus with a hydraulic detention time of 17 days. Daphnia, magna (ecotoxicity) and Allium cepa (phytotoxicity, cytotoxicity, and, genotoxicity) were used as tools to monitor the efficiency of the integrated system. Obtained results showed that the integrated system (MA, + CWs) presented good COD and BOD5 reductions, besides removal rates of, almost 98% for N-NH3, being much more efficient than the UNISC wastewater, treatment plant (UWTTP). The results of ecotoxicity presented the raw wastewaters (RW) as slightly toxic and an absence of ecotoxicity in all the treatments steps. Regarding phytotoxicity, the results showed no significant differences between the treatments. The cytogenetic assays indicated a significant increase in mitotic index (MI) (p < 0.001) after treatment by CWs compared to the final treatment UWTTP while the results, regarding binucleated cells (BNC) did not present significant differences, among the treatments. Micronucleus (MN) indexes were significantly different between the UWWPT and the integrated system (p < 0.01). In relation to chromosome aberrations (CA) the results indicate a significant difference between the CWs and UWWTP treatments (p < 0.01) and, RW and CWs (p < 0.001), confirming the detoxifying potential of the integrated system when compared to UWWPT. Thus, the results of the present research highlight the relevance in the proposition of the integrated system as an alternative of cleaner technology to the detriment of conventional technologies applied in wastewater treatment.

Exposure to mobile phone radiations at 2350MHz incites cyto- and genotoxic effects in root meristems of Allium cepa.

The exponential increase of electromagnetic field radiations (EMF-r) in the natural environment has raked up the controversies regarding their biological effects. Concern regarding the putative capacity of EMF-r to affect living beings has been growing due to the ongoing elevation in the use of high frequency EMF-r in communication systems, e.g. Mobile phones. In the present study, we tried to examine the cyto- and genotoxic potential of mobile phone EMF-r at 2350MHz using onions (Allium cepa L.). Fresh adventitious onion roots were exposed to continuous EMF-r at 2350MHz for different time periods (1h, 2h and 4h). The evaluation of cytotoxicity was done in terms of mitotic index (MI), phase index and chromosomal aberrations. Genotoxicity was investigated employing comet assay in terms of changes in % HDNA (head DNA) and % TDNA (tail DNA), TM (tail moment) and OTM (olive tail moment). Data were analyzed using one-way ANOVA and mean values were separated using post hoc Tukey's test. The results manifested a significant increase of MI and chromosomal aberrations (%) upon 4h, and ≥ 2h of exposure, respectively, as compared to the control. No specific changes in phase index in response to EMF-r exposure were observed. The % HDNA and % TDNA values exhibited significant changes in contrast to that of control upon 2h and 4h of exposure, respectively. However, TM and OTM did not change significantly. Our results infer that continuous exposures of radiofrequency EMF-r (2350MHz) for long durations have a potential of inciting cyto- and genotoxic effects in onion root meristems.

Effect of GINGER AGAINST THE TOXICITY OF BRODIFACOUM ON ALBINO MALE MICE

Brodifacoum is a 4-hydroxycoumarin anticoagulant rodenticide of Rodents. This denotes a high possibility that contaminated the diet of man and his farm animals this fact alarmed some scientists to study their biological influence. Ginger (Zingiber officinale) has been used as an antioxidant and preventing agent against numbers of diseases. The study deals with effects of brodifacoum on the chromosome and on the liver and testis of male mice as well as and the protective effects of ginger extract. 130 Adult albino male mice were used in this study. Group (G1) served as control. Groups (G2&3) was received ginger extract doses 1.5% and 3%. Groups (G4&5) received single dose (1/10 LD50) of brodifacoum for group (G4) and (1/20 LD50) for group (G5). Groups (G6, 7, 8 & 9) received brodifacoum then ginger extract. Groups (G10, 11, 12&13) received ginger extract then brodifacoum. Animals from all the groups were sacrificed and their bone marrow cells were analyzed for chromosomal damage. Liver and testis samples are collected for histopathological examination. The animals of the groups that treated with brodifacoum showed significant increase of chromosomal aberrations and the value of mitotic index were reduced. Treated male mice with brodifacoum induced various histological changes in liver and testis. Treated animals with brodifacoum and ginger lead to reduction in chromosomal aberrations, significant increase in mitotic index and slight improvement in the histological change in liver and testis. Results obtained from this study demonstrated that brodifacoum has a mutagenic effect on chromosome and histopathological change in different organ (liver and testis) and the use of ginger may be a potent protective against the toxicity of brodifacoum anticoagulant rodenticide.

The effect of Larinus maculates F. Cocoon Aqueous Extract in Some Immunological Aspects of Male Albino Mice

Traditional folk medicine is applied in the treatment of diversity chronic and acute conditions all over the worlds. This study was carried out to assess the capability of aqueous beetle cocoon extract (Lorinus maculates F.) commonly known as (Tihan) in stimulating immune response. Adult mice (5 in each group) were divided into two groups: the first one orally administered with 150 mg/kg and the second group received normal saline as a control group for 14 days. Bone marrow and spleen were proceed for mitotic index and phagocytosis estimated. The results showed a significant increase in mitotic index and phagocytosis in treaded mice in comparing with the negative control. These results concluded that Tihan has a potential role in innate immune response.

Feulgen stain as a special stain for mitotic figures and apoptotic bodies in oral squamous cell carcinoma

Background: Oral cancer is one of the most prevalent cancers worldwide. The oral squamous cell carcinoma (OSCC) is graded for the proper treatment planning, and it has been a subjective phenomenon. This grading depends on several features, of which the presence of mitotic figures and apoptotic bodies is one of the important criteria. The routine hematoxylin and eosin (H and E) staining may help in identifying the mitotic figures and apoptotic bodies, but it is difficult to identify accurately. Thus, an attempt was made to evaluate the efficacy of crystal violet and Feulgen stain in identifying the mitotic figures and apoptotic bodies and to observe any variation in different grades of carcinoma. Aims: This study was aimed at using crystal violet and Feulgen stain in identifying mitotic figures and apoptotic bodies in different grades of OSCC. Materials and Methods: Fifteen diagnosed cases of OSCC were retrieved and stained with routine H and E, crystal violet stain, and Feulgen stain. All the sections were scanned for mitotic figures and apoptotic bodies. Apoptotic index (AI) and mitotic index (MI) were calculated. AI and MI were expressed as the average of a total number of apoptotic and mitotic cells counted in ten high-power fields. Results: A significant increase in MI and AI was found in Feulgen stain than crystal violet and H and E stain. Conclusion: Feulgen stain can be considered as the best, cost-effective, relatively cheap stain to visualize mitotic figures and apoptotic bodies.

Effect of Methotrexate and Omega-3 Combination on Cytogenetic Changes of Bone Marrow and Some Enzymatic Antioxidants: An Experimental Study

Effect of Methotrexate and Omega-3 Combination on Cytogenetic Changes of Bone Marrow and Some Enzymatic Antioxidants: An Experimental Study

Effect of Methotrexate and Omega-3 Combination on Cytogenetic Changes of Bone Marrow and Some Enzymatic Antioxidants: An Experimental Study

Effect of Methotrexate and Omega-3 Combination on Cytogenetic Changes of Bone Marrow and Some Enzymatic Antioxidants: An Experimental Study

Estimation of Mutagenic Effect and Modifications of Mitosis by Silver Nanoparticles

We analyzed mutagenic and mitosis-modifying effects of silver nanoparticles (Allium test). Chromosome aberrations and laggings and micronuclei were simultaneously registered in the same sample. Mitotic and phase indexes were calculated. No mutagenic effects were detected after treatment with silver nanoparticles in doses of 1.0, 2.5, 5.0, and 50 mg/liter. Silver nanoparticles in a concentration of 50 mg/liter significantly increased the mitotic index. Nanoparticles in a dose of 5 mg/liter induced slight, but significant increase in mitotic index, but did not affect the ratio of phase indexes. Exposure to silver nanoparticles in concentrations of 1.0 and 2.5 mg/liter was not followed by modification of mitosis.

FISIOLOGY ADAPTATION OF SANDY ANEMONE (Heteractis malu) EXPOSED TO ELEVATED TEMPERATURES: LABORATORY CONDITION

&lt;p&gt;Sandy anemone (Heteractis malu), belongs to Phylum Cnidaria, physiologically is very close to coral stone, which was a major component of coral reef ecosystems. As coral stone, Heteractis malu also has symbiotic algae (Zooxanthella). Physiologically, the alga symbiotic relationship of coral stone is almost similar with Heteractis malu. Maintaining Heteractis malu in the laboratory is relativly easier compared to that of coral stone. Advantages of the Heteractis malu vs. stone coral, its body is not covered by limestone makingit easier in processing analyses. The response of the anemone to stress is expected similar with coral stone. This research aims to analyze the response and adaptation of Heteractis malu to the temperature increase of 1 °C and 2 °C of the normal temperature (28 °C). The impact of temperature increases on Heteractis malu did not significantly affect the density of zooxanthellae, however, there was a significant increase of mitotic index. In addition, during a recovery process, Heteractis malu immune system did not show a significant increase based on its mitotic index results tended to decrease during the second phase of stress treatment.&lt;/p&gt;&lt;p&gt;Keywords: Adaptation, sandy anemone (Heteractis malu), temperature increase,, zooxanthellae&lt;/p&gt;

FISIOLOGY ADAPTATION OF SANDY ANEMONE (Heteractis malu) EXPOSED TO ELEVATED TEMPERATURES: LABORATORY CONDITION

Sandy anemone (Heteractis malu), belongs to Phylum Cnidaria, physiologically is very close to coral stone, which was a major component of coral reef ecosystems. As coral stone, Heteractis malu also has symbiotic algae (Zooxanthella). Physiologically, the alga symbiotic relationship of coral stone is almost similar with Heteractis malu. Maintaining Heteractis malu in the laboratory is relativly easier compared to that of coral stone. Advantages of the Heteractis malu vs. stone coral, its body is not covered by limestone makingit easier in processing analyses. The response of the anemone to stress is expected similar with coral stone. This research aims to analyze the response and adaptation of Heteractis malu to the temperature increase of 1 °C and 2 °C of the normal temperature (28 °C). The impact of temperature increases on Heteractis malu did not significantly affect the density of zooxanthellae, however, there was a significant increase of mitotic index. In addition, during a recovery process, Heteractis malu immune system did not show a significant increase based on its mitotic index results tended to decrease during the second phase of stress treatment.Keywords: Adaptation, sandy anemone (Heteractis malu), temperature increase,, zooxanthellae

Cytotoxic effects of infusions (tea) of Solidago microglossa DC. (Asteraceae) on the cell cycle of Allium cepa

The medicinal specie Solidago microglossa DC., which is native from South America, is used as infusion (tea) for stomach disorders in popular medicine. This study aimed to evaluate the potential cytotoxic effect of these infusions on the cell cycle of Allium cepa. In natura leaves of three populations of this species were used in preparing infusions at two concentrations: 1.75 mg/mL and 14 mg/mL. Onion (Allium cepa) root-tip cells were used as test system in in vivo assays. Slides were done through smashing technique. Cells were analyzed in all cell cycles of A. cepa, totalizing 6000 cells for each group of bulbs. The mitotic index (MI) was calculated, and the statistical analysis was carried out through the Chi-square test (χ2 = 0.05). The results show that the infusions of S. microglossa at a highest concentration (14 mg/mL) caused a significant reduction in the MI compared with control in the studied populations. In concentrations commonly used by people there was a significant increase of MI in relation to controlled populations 1 and 3 and a decrease in population 2. In conclusion, infusions of S. microglossa presented antiproliferative effect at a highest concentration.

Protective effects of lupeol against benzo[a]pyrene induced clastogenicity in mouse bone marrow cells

Fruits and vegetables contain a variety of ingredients that exhibit chemopreventive effects against an array of xenobiotics. In the present study, the antigenotoxic potential of lupeol, a triterpene, and mango pulp extract (MPE) was evaluated in Swiss albino mice. Benzo[a]pyrene (B[a]P), a well-known mutagen, was given at a single dose of 100 mg/kg body weight intraperitoneally. Pretreatment with lupeol (1 mg/animal) and MPE (1 mL, 20%) was given through oral intubation for 7 days prior to B[a]P administration. Animals from all the groups were killed at sampling time of 24 h and their bone marrow tissue was analyzed for chromosomal damage and micronuclei induction. In B[a]P-treated animals a significant induction of chromosomal aberration and micronuclei was recorded, with a decrease in mitotic index. In lupeol- or MPE-supplemented groups, a significant decrease in B[a]P-induced clastogenicity was recorded. The incidence of aberrant cells and micronuclei was found to be reduced by both lupeol and MPE when compared to the B[a]P-treated group. The anti-cytotoxic effects of lupeol or MPE were also evident, as observed by significant increase in mitotic index. Thus, results of the present investigation revealed that lupeol and MPE have protective effects against B[a]P-induced clastogenic changes in Swiss albino mice.

THE PROTECTIVE POTENCY OF GREEN TEA AND GINGER EXTRACTS ON THE GENOTOXIC EFFECT OF MALATHION INSECTICIDE IN BONE MARROW CELLS OF MICE (MUS MUSCULUS)

In present set of investigations the chemoprotective effect of green tea and ginger extracts has been evaluated using in vivo chromosomal aberrations assay in albino mice (Mus musculus). The organophosphate agropesticide malathion, 80% technical grade consider as a potent genotoxic agent, was given at a single dose 230 mg/kg b.w. (1/12 LD50) intraperitoneally. Pretreatment with 4 and 3% of freshly prepared green tea (GTI), ginger (GI) extracts, respectively and the mixture of both extracts (GTI+GI) were given through oral incubation for 6 days prior to malathion administration. Animals from all the groups were sacrified at sampling times of 24 and 48 hours and their bone marrow cells were analyzed for chromosomal damages. The animals of the positive control group (Malathion alone) showed a significant increase in chromosomal aberrations both at 24 and 48 h sampling time. The green tea and ginger extracts, alone did not significantly induced aberrations at either sampling time, conforming their non-mutagenicity. However, significant suppressions in the chromosomal aberrations were recorded following pretreatment with green tea and ginger extracts administration. The antigenotoxic effects of both extracts separately and in mixture were also evident, as observed by significant increase in mitotic index, when compared to positive control group. Reduction in malathion induced clastogenicity by both extracts, was evident at 24 h and to a much greater extent at 48 h of cell cycle. Thus results of the present investigations revealed that green tea and ginger extracts have chemoprotective potential against malathion induced chromosomal mutations in albino mice.

Effects of Power Frequency Electromagnetic Fields on Growth of Germinating Vicia faba L., the Broad Bean

Experimental investigations were carried out to evaluate the effect of continuous and delayed exposure of power frequency electromagnetic fields at 5, 50 and 100 μT on germinating Vicia faba seedlings as a model system. These studies included physical parameters (length and girth of primary roots, number as well as length of lateral roots and imbibition), major biochemical constituents (total sugar, protein, and fat) and activities of important housekeeping enzymes (amylases, proteases, and lipase) at 2, 4, and 8 days of growth. Also, mitotic index and rate of DNA synthesis were studied at day 8 of growth. There was no significant change in physical parameters and major biochemical constituents between control and experimental groups. Also, the comparison between the control and experimental group of seeds showed that α-amylase activity significantly decreased at 5, 50 and 100 μT on day 2 and 4 of growth. β-amylase and protease (37○C & 50○C) showed a significant decrease in activity on day 2 and 4 of growth at 100 μT, whereas activity of lipase significantly decreased only on day 2 of growth at 100 μT. At day 8 of growth, all enzyme activities reverted back to the same as control. Also, there was a significant increase in mitotic index as well as 3H-thymidine uptake at 100 μT delayed exposure on day 8. The present study suggests that exposure to power frequency electromagnetic fields up to 100 μT on germinating seedlings does not cause any permanent damage since the initial alteration under the magnetic fields in some important housekeeping enzymes involved in the onset of seed germination were returened to control values on day 8 of growth. Also, the growth of the germinated seedlings was found to be enhanced by the application of power frequency magnetic fields (100 μT) as evidenced by mitotic index and 3H-thymidine uptake.

Cytogenetic effects of osmotic stress on the root meristem cells of Centaurea ragusina L.

Centaurea ragusina L. (Asteraceae), an endemic Croatian species developed xeromorphic characteristics which have arisen as a consequence of arid- and salt-affected environment. Cytogenetic effects of mannitol (300 mM) and different NaCl concentrations (150, 300, 450 and 600 mM) on root meristem cells of in vitro cultured C. ragusina were analysed after 10 days of stress conditions. Plants grown on media containing 150 mM NaCl showed significant increase of mitotic index and higher number of mitotic abnormalities as compared to those of control conditions. Although the mitotic index in control and mannitol-treated samples was almost the same, mitotic and chromosomal abnormalities doubled in the latter. Higher concentrations of salt (300, 450 and 600 mM NaCl) caused total inhibiton of mitotic activity in root-tip cells. Comparison of isoosmotic concentrations of salt (150 mM) and mannitol (300 mM) revealed more detrimental cytotoxic effect of mannitol and a certain tolerance of C. ragusina towards moderate salinity.

Antimutagenic effects of garlic extract on chromosomal aberrations

Garlic ( Allium sativum) has been used since ancient times, as a spice and also for its medicinal properties. In present set of investigations antimutagenic effect of garlic extract (GE) has been evaluated using ‘in vivo chromosomal aberration assay’ in Swiss albino mice. Cyclophosphamide (CP), a well-known mutagen, was given at a single dose of 25 mg/kg b.w. intraperitoneally. Pretreatment with 1, 2.5 and 5% of freshly prepared GE was given through oral intubation for 5 days prior to CP administration. Animals from all the groups were sacrificed at sampling times of 24 and 48 h and their bone marrow tissue was analyzed for chromosomal damage. The animals of the positive control group (CP alone) shows a significant increase in chromosomal aberrations both at 24 and 48 h sampling time. GE, alone did not significantly induced aberrations at either sampling time, confirming its non-mutagenicity. However in the GE pre-treated and CP post-treated groups, a dose dependent decrease in cytogenetic damage was recorded. A significant suppression in the chromosomal aberrations was recorded following pretreatment with 2.5 and 5% GE administration. The anticytotoxic effects of GE were also evident, as observed by significant increase in mitotic index, when compared to positive control group. Reduction in CP induced clastogenicity by GE was evident at 24 h and to a much greater extent at 48 h of cell cycle. Thus results of the present investigations revealed that GE has chemopreventive potential against CP induced chromosomal mutations in Swiss albino mice.