Al-B4C neutron absorber in spent fuel dry storage could suffer significant radiation damage at elevated temperature (∼150–300 °C) mainly via 10B(n, α)7Li reactions. Several recent studies reported significant bubble formation in the absorber, however only from wet storage environment at near ambient temperature (< ∼90 °C). In this study, we irradiated a commercially widely used Al-B4C absorber of interest utilizing 120-keV helium ion-beam accelerator with twelve different conditions; three different doses (0.01, 0.1, and 1 dpa) combined with four different temperatures (room temperature, 150, 270, and 400 °C). This was to investigate the irradiation-induced microstructural evolution of the absorber in dry storage environment in expedited manner instead of time-consuming neutron irradiation test. The effects of irradiation dose and temperature on helium bubble nucleation and growth were investigated by image analysis on BF-TEM images, which showed a typical tendency of increasing bubble size with increasing temperature at the expense of decreasing bubble number density. Helium bubble morphology at grain boundaries was quite similar with the ones reported from the previous studies on the surveillance coupons used in the wet environment, elliptic helium bubbles around B4C particles. Notable difference was the prominent formation of numerous helium bubbles even from the lowest dose (0.01 dpa) at slightly elevated temperature (150 °C), perhaps due to higher irradiation temperature than that of spent fuel pool. This study may experimentally confirm that significant bubble formation and growth in the absorber could be the case from the early stage of the dry storage, even without the participation of hydrogen produced from the absorber corrosion. Particularly in the case of non-clad Al-B4C absorber, possible B4C particle detachment may need to be concerned since the bubble coalescence was concentrated at the interface between Al alloy matrix and B4C particle which would be microcracked under neutron irradiation.