Rapid changes in vertical illuminance trigger visual fatigue. Therefore, controlling the illuminance ratio of adjacent spaces can ensure the satisfaction and comfort of users. This study takes reaction time as the measure of adaptation and explores the correlation between visual adaptation and comfort in different light environments. The Landolt C ring was selected as the visual standard for the experimental test, the degree of visual comfort was assessed using a Likert scale, and experimental parameters were formulated according to relevant criteria. By analyzing the subjective visual comfort, visual task performance and physiological evaluations of the participants under different changing illuminance levels, we have concluded that there is a significant correlation between reaction time and visual comfort, and no significant effect of gender on visual comfort. Therefore, under the condition of meeting the required value of illumination standard, the smaller the illuminance ratio of adjacent rooms, the more the comfort and visual acuity of users can be guaranteed, and visual fatigue can also be avoided. The study is a useful resource for improving comfort and pleasure in a light environment as well as for lighting design.