(Backgrounds)To detect significant fibrosis in chronic hepatitis is important to determine the timing of treatment intervention. Liver biopsy is still a gold standard until now, though it is quite invasive. In this study, we evaluate the utility of some laboratory tests and indeces that are related to liver fibrosis and found new formulas that is considered to be more effective than previously reported indeces and proposed as one of the noninvasive and easy diagnostic tool. (Patients and Methods) 95 patients with chronic hepatitis who have also done liver biopsy were included in this study. We evaluated the relationship between significant fibrosis (Metavir score≧2) and age, sex, serum AST, ALT, γGTP, hyaruronan, Procollagen-III-peptide (P-III-P), type IV collagen, platelet counts (Plt) as single serum marker, and AST/ALT ratio (AAR), AP index, and AST to platelet ratio index (APRI) as fibrosis indeces. In univariate analysis, Students' t-test was applied in continuous values and Chi-square test was applied in categorical values. In multivariate analysis, logistic regression analysis was done. P<0.05 was considered as statistically significant. And to evaluate the utility of new diagnostic index, ROC analysis was done and AUROCwas evaluated. (Results)In univariate analysis, higher age (p=0.0067), higher AST (p=0.0073), higher hyaruronan (p= 0.0015), higher P-III-P (p<0.0001), higher type IV collagen (p<0.0001), lower Plt (p<0.0001),higher AP index (p<0.0001), and higher APRI (p<0.0001) was selected, and in multivariate analysis, Type IV collagen (p=0.0360) and AP index (p=0.0106) were selected as significant factors. Then we made new scoring system consisted of type IV collagen (≧150:1point,<150:0point) and AP index (≧6: 1 point, <6: 0 point). AUROC of this new score was 0.834. Score 2 points predicted 86.7% (26/30) of patients with significant fibrosis and Score 0 ruled out 96.9% (31/32) of patients with mild fibrosis. (Conclusions)This new diagnostic score consisted of routine laboratory test might be one of the effective and convenient tools to determine the indication of liver biopsy that is quite invasive for the patients, and the timing of treatment intervention in patients with chronic hepatitis.