Significant Species Differences Research Articles

Preclinical Evidence of Mulberry Leaf Polysaccharides on Diabetic Kidney Disease: a Systematic Review and Meta-Analysis.

Mulberry leaf polysaccharides (MLPs) have a variety of biological activities. Preliminary scattered evidence of preclinical studies have reported their potenzial effects on diabetic kidney disease (DKD). Here, we intended to assess the preclinical evidence of MLPs and explore their potenzial mechanisms on DKD, offering a scientific reference for the therapeutic use of MLPs. The study has been registered under the CRD42022309117 registration number at PROSPERO. Comprehensive search was conducted across eight databases from their establishment till January 2024, and eight studies with 270 animals were included in the meta-analysis. The primary outcome measurements in the MLP group, including serum creatinine (Scr) (P = 0.0005), blood urea nitrogen (BUN) (P = 0.02), 24-hour urinary protein (UP) (P = 0.001), and urinary microalbumin (UAlb) (P < 0.0001), were significantly reduced compared to the control group. Additionally, MLP treatment was significantly correlated with fasting blood glucose (FBG), total cholesterol (TC), protein expression of TGF-β1, CTGF mRNA, and the kidney index (all P values < 0.05) and delayed the progression of local pathological changes in the kidney. Subgroup analysis revealed significant species differences in the efficacy of MLPs. Also, it showed that the dosage of streptozotocin potenzially affected the Scr and UAlb results, while the duration of MLP treatment influenced UAlb results. MLPs may exert potenzial renal protection by delaying renal fibrosis, inhibiting inflammatory reactions, suppressing the growth hormone-insulin-like growth factor-insulin-like growth factor binding protein axis, and regulating the insulin receptor pathway. In summary, MLPs have multifaceted renal protective effects, suggesting their potenzial for treating DKD.

Cervical cancer microbiome analysis: comparing HPV 16 and 18 with other HPV types

Differences in the cervicovaginal microbiome may influence the persistence of HPV and therefore, the progression to cervical cancer. We aimed to analyze and compare the metatranscriptome of cervical cancers positive for HPV 16 and 18 with those positive for other HPV types to understand the microbiome’s influence on oncogenicity. RNA sequencing data from a total of 222 invasive cervical cancer cases (HPV16/18 positive (n=42) and HPV “Other types” (n=180)) were subjected to taxonomy classification (Kraken 2) including bacteria, virus and fungi to the level of species. With a median depth of 288,080.5 reads per sample, up to 107 species (38 bacterial, 16 viral and 53 fungal) were identified. Diversity analyses revealed no significant differences in viral or fungal species between HPV16/18 and other HPV types. Bacterial alpha diversity was significantly higher in the "Other HPV types" group for the Observed index (p=0.0074) (but not for Shannon). Cumulative species curves revealed greater species diversity in the “Other HPV types” group compared to “HPV16/18 but no significant differences in species abundance were found between HPV groups. The study did not detect strong significant microbiome differences between HPV 16/18 and other HPV types in cervical cancers. Further research is necessary to explore potential factors influencing the oncogenicity of different HPV types and their interaction with the cervical microbiome.

Characterization of the vaginal microbiota ininfertile women with repeated implantation failure.

Although the relationship between vaginal microorganisms and fertility has been well established, only few studies have investigated vaginal microorganisms in women undergoing in vitro fertilization (IVF). Our aim was to study the differences in vaginal microbiota between infertile women with repeated implantation failure (RIF) and those who achieved clinical pregnancy in their first frozen embryo transfer cycle. We compared the vaginal microbiota of patients with a history of RIF (n = 37) with that of the control group (n = 43). Following DNA extraction, metagenomic sequencing was employed for the analysis of alpha and beta diversities, distinctions in bacterial species, and the functional annotation of microbial genes. Furthermore, disparities between the two groups were revealed. Alpha diversity analysis revealed that the Shannon index was higher in the RIF group (P < 0.05). There were differences in the beta diversity between groups (P = 0.16). At the bacterial family level, the relative abundance of Actinomycetaceae (P = 0.013) and Ruminococcaceae (P = 0.013) were significantly higher in the RIF group. At the genus level, the abundances of Actinomyces (P = 0.028) and Subdoligranulum (P = 0.013) weresignificantly higher in the RIF group. At the species level, the abundances of Prevotella timonensis (P = 0.028), Lactobacillus jensenii (P = 0.049), and Subdoligranulum (P = 0.013) were significantly higher in the RIF group. Significant differences in family, genus, species, alpha and beta diversity were observed in the vaginal microbiota between groups. Notably, among these findings, the Subdoligranulum genus emerged as the most prominent correlating factor.

The occurrence and exposure of microplastics in bivalves from Qingdao, China

This study investigated the regional, seasonal, and species abundance and characteristics of microplastics (MPs) in bivalves from Qingdao, China and assessed the dietary exposure of MPs through bivalve consumption. The average abundance was 1.17 ± 1.07 items/individual or 0.17 ± 0.22 items/g wet weight. Fiber was the dominant shape (91.5 %). The average size of MPs was 995.63 ± 796.59 μm. Rayon, PE, and PET contributed mostly to the MPs composition. There were no significant regional or seasonal differences in MPs abundance (p > 0.05), while there were significant species differences (p < 0.05) when describing the abundance by wet weight. The estimated daily intakes of MPs through bivalve consumption by general population, consumer only population, and coastal residents in China were 3.32 × 10−3, 0.08, and 0.16 μg/kg BW, respectively. The exposure assessment method by converting the quantity of MPs into mass may facilitate the risk characterization in the future.

An Intelligent Fishery Detection Method Based on Cross-Domain Image Feature Fusion

Target detection technology plays a crucial role in fishery ecological monitoring, fishery diversity research, and intelligent aquaculture. Deep learning, with its distinct advantages, provides significant convenience to the fishery industry. However, it still faces various challenges in practical applications, such as significant differences in image species and image blurring. To address these issues, this study proposes a multi-scale, multi-level, and multi-stage cross-domain feature fusion model. In order to train the model more effectively, a new data set called Fish52 (multi-scene fish data set, a data set containing 52 fish species) was constructed, on which the model achieved an mAP (mean average precision is a key measure of model performance) of 82.57%. Furthermore, we compared prevalent one-stage and two-stage detection methods on the Lahatan (single-scene fish data set) and Fish30 data set (a data set containing 30 fish species) and tested them on the F4k (Fish4Knowledge (F4K) is a data set focused on fish detection and identification) and FishNet data set (it is a data set containing 94,532 images from 17,357 aquatic species). The mAP of our proposed model on the Fish30, Lahatan, F4k, and FishNet data sets reaches 91.72%, 98.7%, 88.6%, and 81.5%, respectively, outperforming existing mainstream models. Comprehensive empirical analysis indicates that our model possesses a high generalization ability and reaches advanced performance levels. In this study, the depth of the model backbone is deepened, a novel neck structure is proposed, and a new module is embedded therein. To enhance the fusion ability of the model, a new attention mechanism module is introduced. In addition, in the adaptive decoupling detection head module, introducing classes with independent parameters and regression adapters reduces interaction between different tasks. The proposed model can better monitor fishery resources and enhance aquaculture efficiency. It not only provides an effective approach for fish detection but also has certain reference significance for the identification of similar targets in other environments and offers assistance for the construction of smart fisheries and digital fisheries.

H3K9me3 Levels Affect the Proliferation of Bovine Spermatogonial Stem Cells.

Spermatogonial stem cells (SSCs) possess the characteristics of self-renewal and differentiation, as well as the ability to generate functional sperm. Their unique stemness has broad applications in male infertility treatment and species preservation. In rodents, research on SSCs has been widely reported, but progress is slow in large livestock such as cattle and pigs due to long growth cycles, difficult proliferation in vitro, and significant species differences. Previously, we showed that histone 3 (H3) lysine 9 (K9) trimethylation (H3K9me3) is associated with the proliferation of bovine SSCs. Here, we isolated and purified SSCs from calf testicular tissues and investigated the impact of different H3K9me3 levels on the in vitro proliferation of bovine SSCs. The enriched SSCs eventually formed classical stem cell clones in vitro in our feeder-free culture system. These clones expressed glial cell-derived neurotrophic factor family receptor alpha-1 (GFRα1, specific marker for SSCs), NANOG (pluripotency protein), C-KIT (germ cell marker), and strong alkaline phosphatase (AKP) positivity. qRT-PCR analysis further showed that these clones expressed the pluripotency genes NANOG and SOX2, and the SSC-specific marker gene GFRα1. To investigate the dynamic relationship between H3K9me3 levels and SSC proliferation, H3K9me3 levels in bovine SSCs were first downregulated using the methyltransferase inhibitor, chaetocin, or transfection with the siRNA of H3K9 methyltransferase suppressor of variegation 3-9 homologue 1 (SUV39H1). The EDU (5-Ethynyl-2'-deoxyuridine) assay revealed that SSC proliferation was inhibited. Conversely, when H3K9me3 levels in bovine SSCs were upregulated by transfecting lysine demethylase 4D (KDM4D) siRNA, the EDU assay showed a promotion of cell proliferation. In summary, this study established a feeder-free culture system to obtain bovine SSCs and explored its effects on the proliferation of bovine SSCs by regulating H3K9me3 levels, laying the foundation for elucidating the regulatory mechanism underlying histone methylation modification in the proliferation of bovine SSCs.

Echolocating bats show species-specific variation in susceptibility to acoustic forward masking.

Echolocating bats rely on precise auditory temporal processing to detect echoes generated by calls that may be emitted at rates reaching 150-200 Hz. High call rates can introduce forward masking perceptual effects that interfere with echo detection; however, bats may have evolved specializations to prevent repetition suppression of auditory responses and facilitate detection of sounds separated by brief intervals. Recovery of the auditory brainstem response (ABR) was assessed in two species that differ in the temporal characteristics of their echolocation behaviors: Eptesicus fuscus, which uses high call rates to capture prey, and Carollia perspicillata, which uses lower call rates to avoid obstacles and forage for fruit. We observed significant species differences in the effects of forward masking on ABR wave 1, in which E. fuscus maintained comparable ABR wave 1 amplitudes when stimulated at intervals of <3 ms, whereas post-stimulus recovery in C. perspicillata required 12 ms. When the intensity of the second stimulus was reduced by 20-30 dB relative to the first, however, C. perspicillata showed greater recovery of wave 1 amplitudes. The results demonstrate that species differences in temporal resolution are established at early levels of the auditory pathway and that these differences reflect auditory processing requirements of species-specific echolocation behaviors.

Physiological and immunological assessment of two types of crayfish in aquaculture

Due to the need to develop domestic aquaculture of decapod crustaceans, one of the urgent issues is monitoring the health of these aquatic organisms through the most informative bioindicators — hemolymph indicators. The aim of the study was a comparative physiological and immunological assessment of long-toed (Pontastacus leptodactylus) and Australian red-clawed (Cherax quadricarinatus) river crayfish contained in a closed water use facility. The main hydrochemical parameters generally met the existing requirements. The physiological and immunological condition of crayfish was assessed by hematological and cytochemical analyses. Hemolymph samples were taken in vivo by puncture of the ventral sinus. The total number of hemocytes was determined in the native hemolymph in the Goryaev chamber. The number of cells in five large squares was counted. Lysosomal cationic protein was detected in hemocytes by the cytochemical reaction with bromophenol blue, which we previously modified. Red-clawed crayfish have a significantly 45% higher total number of hemocytes, which indicates its greater immune resistance to pathogens compared with long-toed crayfish. The most significant species differences in the hemogram were noted for agranulocytes (HC I) — in long-fingered crayfish, the indicator is higher with a high confidence coefficient (t). With a high degree of confidence, it is possible to judge a higher proportion of semigranulocytes (HC II) in red-lobed crayfish. The proportion of HC IV of long-fingered crayfish is greater than that of red-clawed crayfish. In red-clawed crayfish, the average cytochemical coefficient of ACC is lower than in long-fingered crayfish. This also confirms its high immune resistance due to the consumption of highly cytotoxic cationic protein in the process of immune protection.

Macrophage galactose-type lectin modulates host immunometabolism during Mycobacterium tuberculosis infection

Abstract Macrophages (Mφ) are the primary replicative niche for Mycobacterium tuberculosis (Mtb), a prominent bacterial pathogen responsible for nearly 1.5 million deaths annually. Appropriate metabolic reprogramming and activation of these cells is a major determinant of host-pathogen interactions and control of infection. Macrophage galactose-type lectin (MGL) is an immunomodulatory CLR primarily expressed on myeloid cells. We previously demonstrated a host-protective role for MGL in Mtb infection, with MGL-1 deficient (MGLKO) mice exhibiting increased bacterial burden and production of pro-inflammatory cytokines. Additionally, more lipid-laden cells were observed in the vicinity of granulomatous TB lesions in MGLKO mice, as compared to WT littermates. Extracellular flux analysis further revealed baseline differences in Mφ metabolism, with MGLKO BMDM exhibiting a glycolytic bias, consistent with the pro-inflammatory bias in vivo. LC-MS/MS lipidomic analysis of Mtb-infected whole-lung homogenate and BMDM revealed significant differences in lipid species between MGLKO and WT, with greater accumulation of DHA and other LCPUFA species observed in MGLKO samples. Importantly, these lipids were also observed to be concentrated within TB lesions of MGLKO and WT mice via mass spectrometry imaging (MSI), highlighting the relevance of these findings to disease outcomes. Taken together, we identify a novel role for MGL as a determinant of host immunometabolic response to Mtb infection.

Differential analysis of serum immunology and gut microbiota in patients with gastrointestinal diseases.

Gastric and intestinal diseases possess distinct characteristics although they are interconnected. The primary objective of this study was to investigate the pathogenesis of gastrointestinal diseases through different analyses of clinical characteristics, serum immunology, and gut microbiota in patients with gastrointestinal diseases. We collected serum samples from 89 patients with gastrointestinal diseases and 9 healthy controls for immunological assessment, stool samples for DNA extraction, library construction, sequencing, as well as clinical data for subsequent analysis. Regarding clinical characteristics, there were significant differences between the disease group and the healthy control (HC) group, particularly in terms of age, cancer antigen 125 (CA125), cancer antigen 199 (CA199), alpha-fetoprotein (AFP), total bilirubin (TBIL) and indirect bilirubin (IBIL). The intestinal disease (ID) group exhibited the highest IL-6 level, which significantly differed from the stomach disease (SD) group (p < 0.05). In comparing the HC with the ID groups, significant differences in abundance were detected across 46 species. The HC group displayed a greater abundance of Clostridiales, Clostridia, Firmicutes, Bifidobacterium, Bifidobacteriaceae, Bifidobacteriales, Actinobacteria, Veillonellaceae, Longum, Copri, Megamonas and Callidus than other species. Similarly, when comparing the HC with the SD groups, significant differences in abundance were identified among 49 species, with only one species that the Lachnospiraceae in the HC group exhibited a higher abundance than others. Furthermore, certain clinical characteristics, such as CA125, CA199, glucose (Glu), creatine kinase-MB (CKMB) and interleukin-22 (IL-22), displayed positive correlations with enriched gut species in the ID and SD groups, while exhibiting a negative correlation with the HC group. The disturbance in human gut microbiota is intimately associated with the development and progression of gastrointestinal diseases. Moreover, the gut microbiota in the HC group was found more diverse than that in the ID and SD groups, and there were significant differences in microbial species among the three groups at different classification levels. Notably, a correlation was identified between specific clinical characteristics (e.g., CA125, CA199, Glu, CKMB and IL-22) and gut microbiota among patients with gastrointestinal diseases.

Differences in diacylglycerol acyltransferases expression patterns and regulation cause distinct hepatic triglyceride deposition in fish

Triglyceride (TAG) deposition in the liver is associated with metabolic disorders. In lower vertebrate, the propensity to accumulate hepatic TAG varies widely among fish species. Diacylglycerol acyltransferases (DGAT1 and DGAT2) are major enzymes for TAG synthesis. Here we show that large yellow croaker (Larimichthys crocea) has significantly higher hepatic TAG level than that in rainbow trout (Oncorhynchus mykiss) fed with same diet. Hepatic expression of DGATs genes in croaker is markedly higher compared with trout under physiological condition. Meanwhile, DGAT1 and DGAT2 in both croaker and trout are required for TAG synthesis and lipid droplet formation in vitro. Furthermore, oleic acid treatment increases DGAT1 expression in croaker hepatocytes rather than in trout and has no significant difference in DGAT2 expression in two fish species. Finally, effects of various transcription factors on croaker and trout DGAT1 promoter are studied. We find that DGAT1 is a target gene of the transcription factor CREBH in croaker rather than in trout. Overall, hepatic expression and transcriptional regulation of DGATs display significant species differences between croaker and trout with distinct hepatic triglyceride deposition, which bring new perspectives on the use of fish models for studying hepatic TAG deposition.

Functional activity of the blood system of two migratory species of the Ural

The presented data on the study of the functional activity of the blood system of migratory species of the fauna of the Urals: Vespertilio murinus [Linnaeus, 1758], and Pipistrellus nathusii Keyserling et [Blasius, 1839]. Multivariate nonparametric analysis of variance of red blood parameters (p 0.05) of migrating bats and the sedentary pond bat showed significant species differences. In bats, a certain genetically determined multidirectionality in the mobilization of emergency regulation mechanisms of the lymphoid blood system of sedentary and migratory species has been noted.

Distribution and influencing factors of macrobenthos on three seagrass beds in the intertidal zone of Shandong province, China

IntroductionThe macrobenthos plays a vital role within the ecosystem of seagrass beds, with its characteristics and spatial distribution serving as indicators of the well-being of the seagrass beds.MethodsIn August 2018, three seagrass beds located in the Yellow River Estuary of Dongying, the west coast of Yantai, and Swan Lake of Weihai, were investigated to compare the ecological influences of seagrass habitat on the benthic environment and macrobenthic community. Within each seagrass bed, porewater, sediment, and macrobenthos were sampled from three separate stations (center of seagrass bed, edge of seagrass bed and bare area).Results and discussionOne-way ANOVA showed significant differences (p &amp;lt; 0.05) in environmental factors and macrobenthos species, abundance, biomass and diversity indices among the three seagrass beds. The present data did not show significant impacts on habitat and macrobenthos in the different coverage areas of seagrass beds at the investigated spatial scales, though crustacea and some carnivores were relatively more inclined to inhabit areas with higher seagrass densities. Aquaculture and eutrophication may trigger the loss of seagrass bed habitats, that affects macrobenthic biodiversity, and conservation measures are needed to protect seagrass bed habitats.

Isolated Cardiac Ryanodine Receptor Function Varies Between Mammals.

Concerted robust opening of cardiac ryanodine receptors' (RyR2) Ca2+ release 1oplasmic reticulum (SR) is fundamental for normal systolic cardiac function. During diastole, infrequent spontaneous RyR2 openings mediate the SR Ca2+ leak that normally constrains SR Ca2+ load. Abnormal large diastolic RyR2-mediated Ca2+ leak events can cause delayed after depolarizations (DADs) and arrhythmias. The RyR2-associated mechanisms underlying these processes are being extensively studied at multiple levels utilizing various model animals. Since there are well-described species-specific differences in cardiac intracellular Ca2+ handing in situ, we tested whether or not single RyR2 function in vitro retains this species specificity. We isolated RyR2-rich heavy SR microsomes from mouse, rat, rabbit, and human ventricular muscle and quantified RyR2 function using identical solutions and methods. The single RyR2 cytosolic Ca2+ sensitivity was similar across these species. However, there were significant species differences in single RyR2 mean open times in both systole and diastole-like solutions. In diastole-like solutions, single rat/mouse RyR2 open probability and frequency of long openings (> 6ms) were similar, but these values were significantly greater than those of either single rabbit or human RyR2s. We propose these in vitro single RyR2 functional differences across species stem from the species-specific RyR2 regulatory environment present in the source tissue. Our results show the single rabbit RyR2 functional attributes, particularly in diastole-like conditions, replicate those of single human RyR2 best among the species tested.

Quantitative, compositional, and immunohistochemical analyses of chondroitin sulfate, dermatan sulfate, and hyaluronan in internal organs of deer (Cervus nippon centralis and C. n. yesoensis) and cattle (Bos taurus)

Chondroitin sulfate (CS) + dermatan sulfate (DS) and hyaluronan (HA) concentrations and the sulfation patterns of CS-DS in the cartilaginous tissues and alimentary canals of Honshu Sika deer, Hokkaido Sika deer, and cattle were investigated in the present study. CS + DS concentrations were high in cartilaginous tissues, namely, the trachea and scapular cartilage region (5– 12 g*), and low in the alimentary canal (~0.3 g*). HA concentrations were low in cartilaginous tissues and the alimentary canal (~0.2 g*). All tissues mainly contained A-type [HexAGalNAc(4-sulfate)] and C-type [HexAGalNAc(6-sulfate)] CS + DS. The ratios of A-type/C-type CS + DS were 1.2– 3.1 and 0.9– 16.4 in cartilaginous tissues and the alimentary canal, respectively. CS + DS predominantly comprised β-D-GlcA and α-L-IdoA in cartilaginous tissues and the alimentary canal, respectively. The alimentary canal characteristically contained up to 14 % highly sulfated E-type [HexAGalNAc(4,6-disulfate)] and D-type [HexA(2-sulfate)GalNAc(6-sulfate)] CS + DS. The specific distributions of CS and DS were immunohistochemically confirmed using CS + DS-specific antibodies. Although the omasum of cattle is more likely to have higher concentrations of CS + DS and HA, no significant species differences were observed in the concentrations or sulfation patterns of CS + DS among species for Honshu Sika deer, Hokkaido Sika deer, and cattle. (*per 100 g of defatted dry tissue).

Comparative Analysis of Microbial Species and Multidrug Resistance Patterns Associated with Lower Urinary Tract Infections in Preterm and Full-Term Births.

The rise of multidrug-resistant organisms has significantly complicated the clinical management of urinary tract infections (UTIs), particularly in the context of pregnancy. This study aimed to identify and analyze the significant differences in microbial species and multidrug resistance patterns associated with UTIs in preterm versus full-term births, determine the bacterial species significantly associated with preterm birth, and describe the antibiotic resistance patterns affecting pregnant women with UTIs. This case-control study was conducted in western Romania and focused on pregnant women with UTIs admitted from 2019 to 2023. Data were retrospectively collected from 308 patients with positive cultures. Statistical analyses, including the Chi-square test, Fisher's exact test, and logistic regression models, were employed to compare the proportions of microbial species and resistance patterns between preterm (n = 126) and full-term (n = 182) birth groups and identify factors independently associated with preterm birth. The study found no significant differences in demographic or lifestyle factors between the groups. However, significant differences were observed in several infection and inflammation markers. The median white blood cell count was higher in the preterm group (12.3 vs. 9.1, p = 0.032), and the median C-reactive protein level was significantly higher in the preterm group (18 vs. 7, p < 0.001). The preterm group exhibited a higher incidence of multidrug-resistant organisms, notably ESBL-producing organisms (19.8% vs. 4.4%, p < 0.001) and carbapenem-resistant Enterobacteriaceae (4.8% with p = 0.003). Notably, the resistance to amoxicillin was significantly higher in the preterm group (20.6% vs. 6.6%, p < 0.001). Significant bacterial associations with preterm births included Group B Streptococcus (OR 2.5, p = 0.001) and Enterobacter spp. (OR 1.8, p = 0.022). The study confirmed significant differences in microbial species and multidrug resistance patterns between UTIs associated with preterm and full-term births. The higher prevalence of certain bacteria and increased resistance to commonly used antibiotics in the preterm group underscore the need for tailored antimicrobial therapies and robust microbial identification in managing UTIs during pregnancy.

Az évjárat hatása magyar szürke szarvasmarha legelő vegetációjára 2022- és 2023-ban ― Esettanulmány

The "Szűzföld" area, managed by the Pro Vértes Public Foundation, has been managed for nature conservation and economic grazing since 1998 with Hungarian grey cattle. In the present work the vegetation data of the pasture were analysed for the years 2022-2023, in order to investigate the effect of the weather conditions in an extremely dry year (2022) and an extremely wet year (2023) using 2 × 2 m coenological records. To analyse the ecological indicators of species, we used Borhidi's relative plant ecological indicators: relative nitrogen demand (NB) and relative soil water and soil moisture (WB) scores. We also assessed the data according to Simon's conservation value categories (TVK) and Borhidi's social behaviour types (SBT). The grassland management assessment of the vegetation was based on the forage value scores of the species used by Klapp et al. The results showed no significant differences in species in the two years, but significant differences in cover values. In the year with rainfall, the proportion of accompanying species decreased, while the proportion of weeds and natural disturbance tolerant species increased. The amount of grasses and legumes increased, which are important for grassland management.

Phenolic compounds and antimicrobial activity of leaves of the genus Sorbus species and natural hybrids

The identification of new sources of biologically active substances is an actual direction of research in recent years, in particular, the demand for antimicrobial compounds and natural preservatives of plant origin is growing. Plants of the genus Sorbus L. are known in folk medicine, but for the rational use of plant material, there is a lack of systematic study of the features of the secondary metabolites’ accumulation and the spectrum of biological activity of plants during vegetation in different environmental conditions. In the present work, the seasonal dynamics of the total content of polyphenols, flavonoids and free phenolic acids in the leaves of rowan plants natural to the flora of Ukraine and introduced in the steppe region were investigated. The highest accumulation of phenolic compounds was found in the leaves of S. domestica, S. latifolia, S. hybrida, S. torminalis and S. aucuparia in the first half of June, and in S. aria and S. intermedia in May, which will serve as a criterion for selecting the best plant material to obtain biologically active compounds. The seasonal dynamics of the content of flavonoids and phenolic acids in the leaves of plants had significant species differences, which require more in-depth studies of the phytocompounds component composition. Antimicrobial activity of leaf ethanol extracts of rowan plant was found to be high against gram-negative bacterial strain Klebsiella pneumoniae B920 (especially the activity of S. aria, S. intermedia and S. aucuparia extracts) and Pseudomonas aeruginosa B907 (especially the activity S. torminalis and S. domestica), clinical gram-positive strains of Staphylococcus epidermidis (the highest activity of S. aria, S. domestica and S. intermedia extracts), as well as against clinical strains of fluconazole-resistant fungus Candida albicans (especially the activity of extracts of S. latifolia S. torminalis and S. intermedia). A conclusion was made about the prospects of using leaf extracts of the genus Sorbus plants as an economical and affordable natural source of biologically active polyphenols with a wide spectrum of antimicrobial activity. Further research may be aimed at elucidating the component composition of rowan leaf extracts and establishing the relationship between individual phytocompounds and the antimicrobial effects of plant extracts.

The potential pathogenic role of gut microbiota in rheumatic diseases: a human-centred narrative review.

A growing amount of evidence suggests that gut microbiota plays an important role in human health, including a possible role in the pathogenesis of rheumatic and musculoskeletal diseases (RMD). We analysed the current evidence about the role of microbiota in rheumatoid arthritis (RA), spondyloarthritis (SpA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). In RA, we found a general consensus regarding a reduction of diversity and a specific bacterial signature, with consistent changes according to the different ethnic and geographical areas. The major pathogenetic role in RA is recognised for P. copri, L. salivarius and Collinsella, even if findings become more heterogeneous when considering established disease. In SpA, we found a relative gut abundance of Akkermansia, Coprococcus, Ruminoccocus and a relative reduction in Bacterioides and Firmicutes spp. Human and preclinical data suggest loss of mucosal barrier, increased permeability and Th1- and Th17-mediated inflammation. Additionally, HLA-B27 seems to play a role in shaping the intestinal microbiota and the consequent inflammation. In SLE, the typical gut microbiota signature was characterised by a reduction in the Firmicutes/Bacteroidetes ratio and by enrichment of Rhodococcus, Eggerthella, Klebsiella, Prevotella, Eubacterium and Flavonifractor, even if their real pathogenic impact remains unclear. In SSc, gastrointestinal dysbiosis is well documented with an increase of pro-inflammatory species (Fusobacterium, Prevotella, Ruminococcus, Akkermansia, γ-Proteobacteria, Erwinia, Trabsulsiella, Bifidobacterium, Lactobacillus, Firmicutes and Actinobacteria) and a reduction of species as Faecalibacterium, Clostridium, Bacteroidetes and Rikenella. In conclusion, seems possible to recognise a distinct gut microbiota profile for each RMD, even if significant differences in bacterial species do exist between different studies and there is a high risk of bias due to the cross-sectional nature of such studies. Therefore longitudinal studies are needed, especially on patients with preclinical and early disease, to investigate the real role of gut microbiota in the pathogenesis of RMD.

Deep octopod habitat in the western North Atlantic characterized by Standard Ecological Classification from videos

AbstractHabitat characterization is important to assess fully the niches of different organisms. There is a large knowledge gap regarding habitat use by deep‐sea benthic incirrate octopods, partly due to their assumed preference for hard‐to‐sample rocky substrata. This study uses observations from in situ videos recorded by remotely operated vehicles (ROVs) deployed from the NOAA Ship Okeanos Explorer and implements the Coastal and Marine Ecological Classification Standard (CMECS) to describe the habitat of three common species of bathyal incirrate octopods living in the western North Atlantic Ocean: Bathypolypus bairdii (Verrill 1873), Graneledone verrucosa (Verrill 1881), and Muusoctopus johnsonianus (Allcock, Strugnell, Ruggiero, &amp; Collins 2006). Significant differences in species' preferences for geoform setting, depth, and substrate type were found. All three species are most likely to be observed by ROV in a submarine canyon and least likely to be seen on a seamount. B. bairdii was found shallower than G. verrucosa and M. johnsonianus. This is the first study of its kind using CMECS to classify the habitat of specific organisms as opposed to the habitat types in a specific area.