Objective To compare the differences in physical activity (PA), self-efficacy, stages of change and physical exercise behaviour in college students. Methods International physical activivty questionnaire( IPAQ), scales of self-efficacy, questionnaire of stage of change in physical exercise behaviour, and scales of physical behaviour were used to make a study on college students from Zhejiang province. Results The rate of males (40.43%) was signficant higher than that of females (26.09%) MET in the high activity tertile. There was significantly more energy expenditure in males (( 3462.79 ± 976.31 ) MET) than in females (( 3118.63 ±944.29 ) MET) (P < 0.05 ). The rate of males (44.68 % ) was more than females (25.00%) in maintainanee period and period of action. PA characteristics were shown significant defferences in different stages (F= 31. 380, F=6.012, P < 0.01 ). Characteristics in stages of change were shown high in behaviour score (F= 13.071, F= 7. 461,P <0. 01 ). Four factors as attitude,cognitive controls, behavior disposition and self-efficacy in males were entered into regression model of MET( t = 2.138 ~ 3. 789, P < 0.05 ), while in females only the factor of behaviour attitude was entered. Conclusion There is significant difference in PA, stages of change, behaviour characteristics, and self-efficacy between male and female college students. Key words: Male and female college student; Physical activity; Behavioral science; Comparative study