PURPOSE This study aimed to identify ACTN3 gene polymorphisms amongprofessional ssireum players by weight class and between elite and non-elite players,and to select genotype that matches the characteristics of the sport. METHODS The subjects of this study were 148 male athletes currently working as professionalssireum players. Chi-square test cross-tabulation analysis was conducted to examinethe differences in ACTN3 genotypes between weight classes and between elite andnon-elite players. RESULTS There were no significant differences in allele or genotypebetween ssireum players, but there were significant differences in R-allele and XXgenotype. CONCLUSIONS Players with the R-allele type of the ACTN3 gene weremore often classified as elite. Using this marker as a basis for organizing a playerselection and training programs would be more effective in training those that matchthe characteristics of elite players of the game.